##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l02n02 tsrexp_lib#7.3420000000d07e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14352504 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.58673329754863 33.0 33.0 33.0 33.0 33.0 2 32.54554306342642 33.0 33.0 33.0 33.0 33.0 3 32.533904397448694 33.0 33.0 33.0 33.0 33.0 4 36.41829948279408 37.0 37.0 37.0 37.0 37.0 5 36.40379448770751 37.0 37.0 37.0 37.0 37.0 6 36.41177386189894 37.0 37.0 37.0 37.0 37.0 7 36.4465024360906 37.0 37.0 37.0 37.0 37.0 8 36.4480157608735 37.0 37.0 37.0 37.0 37.0 9 36.42548944769498 37.0 37.0 37.0 37.0 37.0 10-11 36.406478374783944 37.0 37.0 37.0 37.0 37.0 12-13 36.408985707302364 37.0 37.0 37.0 37.0 37.0 14-15 38.7683803118954 40.0 40.0 40.0 37.0 40.0 16-17 38.74280038521501 40.0 40.0 40.0 37.0 40.0 18-19 38.7451501842466 40.0 40.0 40.0 37.0 40.0 20-21 38.69339249095489 40.0 40.0 40.0 37.0 40.0 22-23 38.70055859242402 40.0 40.0 40.0 37.0 40.0 24-25 38.681168108366315 40.0 40.0 40.0 37.0 40.0 26-27 38.65732024878725 40.0 40.0 40.0 37.0 40.0 28-29 38.61910956443558 40.0 40.0 40.0 37.0 40.0 30-31 38.60063832763956 40.0 40.0 40.0 37.0 40.0 32-33 38.521437618132694 40.0 40.0 40.0 37.0 40.0 34-35 38.507631420970164 40.0 40.0 40.0 37.0 40.0 36-37 38.469947125602616 40.0 40.0 40.0 37.0 40.0 38-39 38.44249947604961 40.0 40.0 40.0 37.0 40.0 40-41 38.39167667188945 40.0 37.0 40.0 37.0 40.0 42-43 38.33771573935809 40.0 37.0 40.0 37.0 40.0 44-45 38.27937877599616 40.0 37.0 40.0 37.0 40.0 46-47 38.19331536155642 40.0 37.0 40.0 37.0 40.0 48-49 38.0797764975366 40.0 37.0 40.0 37.0 40.0 50-51 36.703844151515305 38.5 37.0 38.5 35.0 40.0 52-53 37.01849112879536 38.5 37.0 40.0 33.0 40.0 54-55 37.664959821645056 40.0 37.0 40.0 33.0 40.0 56-57 37.60735039683668 40.0 37.0 40.0 33.0 40.0 58-59 37.40403730248046 38.5 37.0 40.0 33.0 40.0 60-61 37.20234901171252 37.0 37.0 40.0 33.0 40.0 62-63 36.97180112264731 37.0 37.0 40.0 33.0 40.0 64-65 36.69695998691239 37.0 37.0 40.0 33.0 40.0 66-67 36.45960506264272 37.0 37.0 40.0 33.0 40.0 68-69 36.200562250322314 37.0 37.0 37.0 33.0 40.0 70-71 35.959100098491525 37.0 37.0 37.0 33.0 40.0 72-73 35.67865443549084 37.0 37.0 37.0 33.0 38.5 74-75 35.53143343489052 37.0 37.0 37.0 33.0 37.0 76-77 35.32407522757005 37.0 37.0 37.0 33.0 37.0 78-79 35.22808344104973 37.0 37.0 37.0 33.0 37.0 80-81 35.130548753025955 37.0 37.0 37.0 33.0 37.0 82-83 35.0520578848123 37.0 37.0 37.0 33.0 37.0 84-85 35.00168465377192 37.0 33.0 37.0 33.0 37.0 86-87 34.940739817943964 37.0 33.0 37.0 33.0 37.0 88-89 34.858307268195155 37.0 33.0 37.0 33.0 37.0 90-91 34.79728913505267 37.0 33.0 37.0 33.0 37.0 92-93 34.768959061080906 37.0 33.0 37.0 33.0 37.0 94-95 34.70729044910909 37.0 33.0 37.0 33.0 37.0 96-97 34.632688728043554 37.0 33.0 37.0 33.0 37.0 98-99 34.56826056275616 37.0 33.0 37.0 33.0 37.0 100-101 32.71104564750513 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17154687508899968 2306 2 0.22468273995739452 2306 3 0.23040843935640964 2306 4 0.26466860568120865 2306 5 0.2928149725510423 2306 6 0.3339954644534515 2306 7 0.31633587040295197 2306 8 0.3137407053185015 2306 9 0.3175843031797356 2306 10-11 0.28957361569120366 2306 12-13 0.30739379609105555 2306 14-15 0.5391168154324362 2306 16-17 0.5568914097006328 2306 18-19 0.5522117895774912 2306 20-21 0.6059293003439805 2306 22-23 0.5731265930735745 2306 24-25 0.5597440152712991 2306 26-27 0.601886967645143 2306 28-29 0.636214898847804 2306 30-31 0.6275169627263821 2306 32-33 0.6910390815265046 2306 34-35 0.6719916493743412 2306 36-37 0.6734462127500009 2306 38-39 0.6156061235708066 2306 40-41 0.7079822162071494 2306 42-43 0.655791090996253 2306 44-45 0.7129185865787377 2306 46-47 0.6679295434841421 2306 48-49 0.6587747908026316 2306 50-51 0.6202493679767898 2306 52-53 0.701909986673968 2306 54-55 0.7260305628370105 2306 56-57 0.6526755806250648 2306 58-59 0.7096477473730474 2306 60-61 0.7054190288758093 2306 62-63 0.7090428478425181 2306 64-65 0.6920516260704161 2306 66-67 0.6781146976051886 2306 68-69 0.6914315883967603 2306 70-71 0.6838591520192949 2306 72-73 0.7697086776630542 2306 74-75 0.7607392455246398 2306 76-77 0.771402638462952 2306 78-79 0.8056567395485956 2306 80-81 0.7887954389157841 2306 82-83 0.7827315665355528 2306 84-85 0.7890814174702143 2306 86-87 0.8255316170750078 2306 88-89 0.9093862995772426 2306 90-91 0.9264265435976142 2306 92-93 0.8945324381322877 2306 94-95 0.869254287105079 2306 96-97 0.8622122447490597 2306 98-99 0.7902893987753004 2306 100-101 0.8452427564569547 2307 1 0.1518005938757696 2307 2 0.1851814133804197 2307 3 0.2137095784816907 2307 4 0.2595587447670695 2307 5 0.26378170145200386 2307 6 0.27238820544465625 2307 7 0.24401060908499517 2307 8 0.2592051526871657 2307 9 0.2729587415186714 2307 10-11 0.2972722597976656 2307 12-13 0.2893370817802392 2307 14-15 0.48146165860747914 2307 16-17 0.45679876733260727 2307 18-19 0.49082552440265914 2307 20-21 0.5372312175524669 2307 22-23 0.5266510131390163 2307 24-25 0.5041720055314372 2307 26-27 0.5338551047572935 2307 28-29 0.5619389639507091 2307 30-31 0.5447250183386672 2307 32-33 0.634654630217824 2307 34-35 0.5903560551927498 2307 36-37 0.6127529775931393 2307 38-39 0.5980317264667221 2307 40-41 0.6169505016116119 2307 42-43 0.5604439472076237 2307 44-45 0.5488044386225894 2307 46-47 0.6242298396859454 2307 48-49 0.627645587552756 2307 50-51 0.6034237519635468 2307 52-53 0.6419931974677056 2307 54-55 0.5978668640110669 2307 56-57 0.5566936739730366 2307 58-59 0.6378119060761165 2307 60-61 0.5819975300341582 2307 62-63 0.6227587987866698 2307 64-65 0.6509936394890516 2307 66-67 0.5611047888421652 2307 68-69 0.6007512945675373 2307 70-71 0.6340084351177993 2307 72-73 0.6744432282775037 2307 74-75 0.6349063051133825 2307 76-77 0.6670576856397261 2307 78-79 0.7027674423027293 2307 80-81 0.7238145598122543 2307 82-83 0.7675292084397256 2307 84-85 0.7458670887004928 2307 86-87 0.7942733231849672 2307 88-89 0.8491688927848955 2307 90-91 0.8348269048250785 2307 92-93 0.7618057830543705 2307 94-95 0.7521595659238898 2307 96-97 0.7658510718909497 2307 98-99 0.8556458482137543 2307 100-101 0.7750892468227804 2201 1 0.1871317223914417 2201 2 0.18662578830609533 2201 3 0.21385547325172638 2201 4 0.2657568297792139 2201 5 0.24538760284951877 2201 6 0.26369400038158375 2201 7 0.22849010297796468 2201 8 0.24328734012996023 2201 9 0.2776676267523541 2201 10-11 0.28070916352482556 2201 12-13 0.28840930962928013 2201 14-15 0.48966333311532395 2201 16-17 0.4841783803465205 2201 18-19 0.48835621157621034 2201 20-21 0.5553441117105322 2201 22-23 0.5487079097610064 2201 24-25 0.5372232359746931 2201 26-27 0.5522596005543221 2201 28-29 0.5451415028036521 2201 30-31 0.5343977856392641 2201 32-33 0.6183553784149751 2201 34-35 0.6084043844429274 2201 36-37 0.6423217533230599 2201 38-39 0.596356796883704 2201 40-41 0.6019058341782042 2201 42-43 0.6124494512068352 2201 44-45 0.6354443755913763 2201 46-47 0.6176212011956395 2201 48-49 0.5837781952927514 2201 50-51 0.4773548034481152 2201 52-53 0.6141393429395663 2201 54-55 0.6106133756479153 2201 56-57 0.651606771381644 2201 58-59 0.6677287059716264 2201 60-61 0.6414664868506392 2201 62-63 0.6581542207975204 2201 64-65 0.6517382185003839 2201 66-67 0.6571699392499042 2201 68-69 0.6562397334717858 2201 70-71 0.6609627652946202 2201 72-73 0.7589844455960773 2201 74-75 0.672011199193939 2201 76-77 0.7546737479774563 2201 78-79 0.7467205174671818 2201 80-81 0.7439159904996302 2201 82-83 0.747850492607661 2201 84-85 0.7443355798562763 2201 86-87 0.7588161130270166 2201 88-89 0.7855076535094483 2201 90-91 0.7526828776250198 2201 92-93 0.7587282865418103 2201 94-95 0.7787330623141813 2201 96-97 0.7556943594454495 2201 98-99 0.7455438588337557 2201 100-101 0.7898307880160473 1101 1 0.1929920785202981 1101 2 0.2122893529804486 1101 3 0.2116277315817925 1101 4 0.27607657647473616 1101 5 0.2721257586274035 1101 6 0.29967475303656244 1101 7 0.265066794164035 1101 8 0.282878819858297 1101 9 0.31153533040392745 1101 10-11 0.29856662498642805 1101 12-13 0.3047366077148652 1101 14-15 0.5506334982229717 1101 16-17 0.5266477919472052 1101 18-19 0.5381707254923 1101 20-21 0.5838807328680389 1101 22-23 0.5786189065920695 1101 24-25 0.5708898211774098 1101 26-27 0.5452779740636302 1101 28-29 0.615260698808406 1101 30-31 0.5606878551146011 1101 32-33 0.6668018788675241 1101 34-35 0.6227407084298946 1101 36-37 0.6280305871963492 1101 38-39 0.6169714984020018 1101 40-41 0.6191444669835207 1101 42-43 0.5973702008909356 1101 44-45 0.6470302539470296 1101 46-47 0.609126042975781 1101 48-49 0.6292498784790297 1101 50-51 0.6276537166719933 1101 52-53 0.6182322840398911 1101 54-55 0.7096924007968965 1101 56-57 0.6929608771923839 1101 58-59 0.697419307549012 1101 60-61 0.708132472345909 1101 62-63 0.6806916063883932 1101 64-65 0.6821075537822097 1101 66-67 0.6433200995776005 1101 68-69 0.6699345461406665 1101 70-71 0.6681098092458697 1101 72-73 0.7718730383194767 1101 74-75 0.7144504248742507 1101 76-77 0.7393534943797633 1101 78-79 0.744526523172155 1101 80-81 0.7675720358715878 1101 82-83 0.7627243023211534 1101 84-85 0.7794609091697993 1101 86-87 0.8174428064813526 1101 88-89 0.8629988356246727 1101 90-91 0.8567653314519674 1101 92-93 0.7371624864911368 1101 94-95 0.7926340417072595 1101 96-97 0.796899402824657 1101 98-99 0.7802980534664812 1101 100-101 0.7813971426854707 2304 1 0.15412878144528008 2304 2 0.18434293076566632 2304 3 0.19748338179113034 2304 4 0.24957413596565203 2304 5 0.25675205689753255 2304 6 0.2275729508312594 2304 7 0.24426878705732946 2304 8 0.2625984598005715 2304 9 0.25141987406166777 2304 10-11 0.25843304890437224 2304 12-13 0.2676993754903947 2304 14-15 0.45580938414710914 2304 16-17 0.45517063023779514 2304 18-19 0.48642414063372286 2304 20-21 0.5138347776389409 2304 22-23 0.47555536269581467 2304 24-25 0.46484964976901466 2304 26-27 0.517730102999252 2304 28-29 0.5115027776827716 2304 30-31 0.45318693498440865 2304 32-33 0.5965698045270429 2304 34-35 0.5934907871369361 2304 36-37 0.5831553916546639 2304 38-39 0.6043305287610465 2304 40-41 0.6164626264887261 2304 42-43 0.5578743993428006 2304 44-45 0.5808435305951392 2304 46-47 0.6132653281258982 2304 48-49 0.5921579871276208 2304 50-51 0.6214946302664188 2304 52-53 0.6405364147180279 2304 54-55 0.6601591966181815 2304 56-57 0.6344807020248382 2304 58-59 0.6549736548827028 2304 60-61 0.6658121200469012 2304 62-63 0.6133994410655887 2304 64-65 0.664286817060308 2304 66-67 0.6504586323872914 2304 68-69 0.6058903902972048 2304 70-71 0.617578485454743 2304 72-73 0.7027915939179579 2304 74-75 0.6557267878487707 2304 76-77 0.7250219714655586 2304 78-79 0.7242117769021803 2304 80-81 0.7104815271449283 2304 82-83 0.7416015772419442 2304 84-85 0.748915935846469 2304 86-87 0.7361255143459289 2304 88-89 0.8075833091973266 2304 90-91 0.765861357700544 2304 92-93 0.7234970917854042 2304 94-95 0.7251800219784883 2304 96-97 0.7465728414521635 2304 98-99 0.7452847039913664 2304 100-101 0.8806343608145895 2202 1 0.13085132277556255 2202 2 0.15483123017890676 2202 3 0.1751198728129566 2202 4 0.20636894775899606 2202 5 0.21474644908894902 2202 6 0.20696986640466974 2202 7 0.2050063997094398 2202 8 0.2126266681569433 2202 9 0.20705902937013576 2202 10-11 0.23066211853339524 2202 12-13 0.23340588906255277 2202 14-15 0.4007930082151603 2202 16-17 0.40912646190141544 2202 18-19 0.4184762534622948 2202 20-21 0.4652066278618463 2202 22-23 0.403795246442364 2202 24-25 0.4383755363424129 2202 26-27 0.43593678786280066 2202 28-29 0.486489823359058 2202 30-31 0.4637060528709185 2202 32-33 0.5167180687663446 2202 34-35 0.5357819649945839 2202 36-37 0.502481720932586 2202 38-39 0.5205171355832405 2202 40-41 0.5244344198202171 2202 42-43 0.5191598675400613 2202 44-45 0.5541706914604632 2202 46-47 0.5296965031911824 2202 48-49 0.5314232741658458 2202 50-51 0.4874847557065678 2202 52-53 0.5992098012467153 2202 54-55 0.5905466709369946 2202 56-57 0.5628610275718415 2202 58-59 0.5298373536132388 2202 60-61 0.5845970946770578 2202 62-63 0.5747680561360298 2202 64-65 0.5659963275857223 2202 66-67 0.5720854156309159 2202 68-69 0.5463543017191697 2202 70-71 0.5469589602191007 2202 72-73 0.6551751248689044 2202 74-75 0.5357796268262618 2202 76-77 0.5977961459557619 2202 78-79 0.6377949853031168 2202 80-81 0.6738043146073522 2202 82-83 0.6691097539994715 2202 84-85 0.6082517664562488 2202 86-87 0.6281702870943349 2202 88-89 0.6327602272297455 2202 90-91 0.7004649804489134 2202 92-93 0.6957248691467868 2202 94-95 0.6601491257054306 2202 96-97 0.7319026038895942 2202 98-99 0.6692938881110706 2202 100-101 0.7019382340677112 1102 1 0.017448942054983263 1102 2 0.07498145652404986 1102 3 0.07391380718788554 1102 4 0.10937870309631137 1102 5 0.07927775098516321 1102 6 0.09050352289932562 1102 7 0.09821588525151981 1102 8 0.09935644062588977 1102 9 0.13487808707402138 1102 10-11 0.12253750190183865 1102 12-13 0.12346216785149267 1102 14-15 0.15455586846130842 1102 16-17 0.1549891075213381 1102 18-19 0.21978615659802614 1102 20-21 0.21692155438131522 1102 22-23 0.143512888943242 1102 24-25 0.19516373706782275 1102 26-27 0.23065238306097768 1102 28-29 0.2176315912590283 1102 30-31 0.2611793267563556 1102 32-33 0.3189592642629222 1102 34-35 0.20101906548509163 1102 36-37 0.22619681812738435 1102 38-39 0.2219401111273882 1102 40-41 0.25755168459173916 1102 42-43 0.2513049039127395 1102 44-45 0.29301511528497315 1102 46-47 0.2755554228003021 1102 48-49 0.30005814594098723 1102 50-51 0.2887537328204459 1102 52-53 0.333517065725772 1102 54-55 0.3281151263692976 1102 56-57 0.30677522996314366 1102 58-59 0.31435157195026164 1102 60-61 0.3049273113826487 1102 62-63 0.2904191745202027 1102 64-65 0.33360223944273315 1102 66-67 0.3273544255977967 1102 68-69 0.34060067997657484 1102 70-71 0.3232100284423609 1102 72-73 0.38735296415924836 1102 74-75 0.30847525942076715 1102 76-77 0.4210113066529999 1102 78-79 0.35932480721182714 1102 80-81 0.38206094420221604 1102 82-83 0.3748311437454106 1102 84-85 0.3379629665642625 1102 86-87 0.3595384802635664 1102 88-89 0.42403054450556965 1102 90-91 0.4034721716497529 1102 92-93 0.37546054874512436 1102 94-95 0.47100891471706063 1102 96-97 0.43942110273192725 1102 98-99 0.43743945053236644 1102 100-101 0.5291024954541825 2305 1 0.11417348569492702 2305 2 0.14523459057095778 2305 3 0.15606044481134518 2305 4 0.15760625611279977 2305 5 0.1916059718359051 2305 6 0.17836942212843354 2305 7 0.18535053457204498 2305 8 0.1698897837998672 2305 9 0.20713354364684733 2305 10-11 0.16562756344578844 2305 12-13 0.1731755672900519 2305 14-15 0.3037513622424157 2305 16-17 0.3130724933824496 2305 18-19 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-0.9718282947637604 1213 74-75 -1.1225098882302547 1213 76-77 -1.0172954293673087 1213 78-79 -1.133294673149777 1213 80-81 -1.1185829072288342 1213 82-83 -1.082938109231442 1213 84-85 -1.0751882052911412 1213 86-87 -1.1251052927608356 1213 88-89 -1.2111058122275367 1213 90-91 -1.3322660274638096 1213 92-93 -1.208255027964853 1213 94-95 -1.1242203154559647 1213 96-97 -1.171253566683589 1213 98-99 -1.3192034042338179 1213 100-101 -1.3677321327418355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19420.0 3 1486.0 4 1004.0 5 801.0 6 816.0 7 2404.0 8 4066.0 9 6237.0 10 10334.0 11 14228.0 12 13716.0 13 12350.0 14 12155.0 15 12505.0 16 13475.0 17 15081.0 18 17041.0 19 19319.0 20 21573.0 21 24391.0 22 27839.0 23 32015.0 24 36242.0 25 42100.0 26 49255.0 27 59188.0 28 71884.0 29 88595.0 30 112379.0 31 146490.0 32 196509.0 33 271710.0 34 383763.0 35 578845.0 36 1202170.0 37 5459179.0 38 5369253.0 39 2686.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.533200145554616 14.260185996691103 21.725575947811386 40.4810379099429 2 25.69468683146829 24.100998643066852 30.80404325048221 19.40027127498265 3 24.036816199183466 26.225551033994517 30.422345125873118 19.315287640948895 4 25.554356161439536 29.96055489693545 21.894139475037523 22.590949466587492 5 28.058040571977127 33.20490365820071 21.598959924832275 17.13809584498988 6 19.11867306899201 37.4173651328208 22.203331284049572 21.260630514137617 7 18.27384809168163 15.575192646209619 43.58112424786419 22.569835014244568 8 22.31433958435757 21.30799971364454 28.40559598845591 27.97206471354198 9 24.55657268246517 22.584774922284712 26.761914134202126 26.096738261047996 10-11 24.276900128774987 30.85662088744776 22.014975464653304 22.851503519123952 12-13 22.80950216186552 24.88574631069385 28.107244771567274 24.19750675587336 14-15 23.209418305366817 26.395450709151287 27.975691926000028 22.419439059481867 16-17 24.064952440879267 26.430050509978027 26.955895478460036 22.549101570682677 18-19 23.857524798890747 26.66794824865818 26.859920296210177 22.6146066562409 20-21 23.806949698030934 27.021355589058405 26.70617880425823 22.465515908652428 22-23 23.96534096803782 26.830526427936004 26.843068870822684 22.361063733203494 24-25 23.565824341833373 27.19915474315585 26.8781344592255 22.356886455785276 26-27 23.905458009290253 27.346667867684708 26.457285154547954 22.290588968477078 28-29 24.006982542596337 26.69444115748803 26.84091957056176 22.45765672935387 30-31 23.58549404140637 26.95755959683862 26.936864330071504 22.520082031683504 32-33 23.75096686410789 27.040539981717476 26.836598613508585 22.371894540666045 34-35 24.09722482505312 26.642043130239934 26.865207234080778 22.395524810626167 36-37 23.659016052915405 26.88236884711997 27.03917213448187 22.419442965482755 38-39 24.08170021523788 26.960909095794992 26.77314393535144 22.18424675361569 40-41 24.182375683689045 26.513800405653086 26.80466379904235 22.499160111615517 42-43 23.88800092767309 26.686811861328092 27.004390494939212 22.420796716059606 44-45 24.255375213091593 26.789558429021326 26.71205756827874 22.243008789608336 46-47 24.210427156418096 26.701309095848725 26.73313653535268 22.355127212380502 48-49 24.048266833714855 26.640211628203442 26.89510304088622 22.41641849719548 50-51 24.29542378076803 26.892995344290664 26.422924707326413 22.388656167614894 52-53 24.443002142387364 26.722667985373853 26.632963606075567 22.201366266163216 54-55 24.073669010729283 26.871268691542728 26.80394572921375 22.25111656851424 56-57 24.462032776942184 26.93365756793832 26.674760204009896 21.929549451109605 58-59 24.77409674290061 26.926298268510052 26.50212510555738 21.797479883031954 60-61 24.269117251686094 27.042585794523035 26.42898229806724 22.25931465572364 62-63 25.018456474887262 27.077609442011568 26.15166658828189 21.75226749481928 64-65 25.021124916918897 27.054714424694197 26.267563064341676 21.656597594045227 66-67 24.955199118325627 27.20711216395849 26.271216074783183 21.5664726429327 68-69 25.40489067970844 27.23872629367016 26.00541462077446 21.35096840584694 70-71 25.540800707799054 27.30398219670701 25.837750243651392 21.31746685184254 72-73 25.576049016606206 27.34260131179664 25.723349827315555 21.3579998442816 74-75 25.944194778912472 27.421587509718105 25.540186957649485 21.094030753719935 76-77 26.281672580578814 27.336654119493126 25.570725055849085 20.81094824407897 78-79 26.32327532271363 27.3305369380582 25.54502265754704 20.801165081681127 80-81 26.774189021994143 27.48658652427466 25.042160028767324 20.697064424963877 82-83 27.095664290608536 27.400993971989507 25.05386713475975 20.44947460264221 84-85 27.09304270947108 27.380062064162097 25.135099237260327 20.39179598910649 86-87 27.476725880695046 27.498723301650973 24.85803131806041 20.16651949959357 88-89 27.876221535199182 27.369273348209994 24.766569894404753 19.98793522218607 90-91 27.975660107572143 27.350215596931093 24.802597053692992 19.871527241803772 92-93 28.439862957370565 27.528775267339483 24.325160939668248 19.706200835621704 94-95 28.68989324725777 27.470452580949768 24.43783673279546 19.401817438997 96-97 28.62277519384119 27.656295401336518 24.4860448755512 19.234884529271092 98-99 29.194542801155237 27.65609350149743 24.202340663698624 18.94702303364871 100-101 29.42891682675624 27.61855628194631 24.040921983353243 18.911604907944206 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 16627.0 1 9123.5 2 1299.0 3 821.0 4 587.0 5 513.0 6 524.5 7 518.0 8 508.5 9 510.0 10 497.5 11 506.0 12 558.5 13 601.5 14 623.0 15 664.0 16 735.0 17 834.5 18 978.5 19 1183.0 20 1409.0 21 1651.5 22 2077.0 23 2769.5 24 3905.5 25 5548.0 26 8153.0 27 12161.5 28 18033.5 29 25723.5 30 35933.5 31 48568.0 32 62774.0 33 79696.0 34 99932.0 35 124596.0 36 158999.0 37 202871.5 38 254539.5 39 319876.5 40 400844.0 41 493073.0 42 591573.5 43 693703.5 44 791583.5 45 873873.0 46 932364.5 47 959217.5 48 955632.0 49 924593.0 50 864326.0 51 783400.5 52 687216.0 53 586771.0 54 488025.0 55 400940.5 56 328730.5 57 271149.0 58 224052.0 59 178819.5 60 139418.5 61 99282.5 62 63519.0 63 40723.5 64 26247.5 65 16265.5 66 10828.5 67 6824.0 68 4671.0 69 3744.5 70 3238.5 71 2546.5 72 1536.0 73 1075.0 74 984.0 75 843.0 76 638.0 77 321.5 78 154.0 79 116.5 80 94.0 81 73.0 82 63.5 83 48.0 84 38.0 85 42.5 86 34.5 87 22.5 88 20.5 89 16.5 90 13.0 91 10.0 92 8.5 93 7.5 94 5.5 95 4.0 96 4.0 97 2.5 98 2.5 99 1.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16625670335991544 2 0.1509004979200842 3 0.15606336009382055 4 0.14766761256433023 5 0.17472909256809824 6 0.1553317804335745 7 0.1566207541206747 8 0.14398881198709299 9 0.17114783594556043 10-11 0.1430273072907696 12-13 0.13272945264463956 14-15 0.19253783172608765 16-17 0.19996859084658677 18-19 0.18435807438200333 20-21 0.21832427289342682 22-23 0.20301683943094528 24-25 0.19414033955329327 26-27 0.1795470671877186 28-29 0.19654410129410171 30-31 0.14439640636923007 32-33 0.1714787886490051 34-35 0.17526558431894532 36-37 0.17861691590540577 38-39 0.17812222870657274 40-41 0.2136247445045129 42-43 0.19851936637676604 44-45 0.18262666918608766 46-47 0.18683151037616852 48-49 0.2263333283167871 50-51 0.20440684078541277 52-53 0.16951397470434426 54-55 0.155558221756984 56-57 0.15139170140624938 58-59 0.16585259269044622 60-61 0.16120183627888207 62-63 0.14972997046369052 64-65 0.17165645799506482 66-67 0.17571149954042864 68-69 0.18705446798691017 70-71 0.1682180335919084 72-73 0.1766904228000912 74-75 0.2090854668983196 76-77 0.23694123339035475 78-79 0.2191115919563583 80-81 0.19450961309608414 82-83 0.16533700321560613 84-85 0.17469425544141984 86-87 0.16409331779318787 88-89 0.167343621712281 90-91 0.1787388458487801 92-93 0.16395745299914216 94-95 0.15756484025365888 96-97 0.165124496742868 98-99 0.1553387478589102 100-101 0.16224695007923357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4352504E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.18979398100434 #Duplication Level Percentage of deduplicated Percentage of total 1 69.22320563736422 28.5128957890773 2 14.730926349630852 12.135276429812864 3 5.784337996894061 7.1476707122568515 4 2.865555302838594 4.721265302603848 5 1.7422724654482313 3.588192195529458 6 1.092971954361631 2.7011573776302757 7 0.8215658824955921 2.3688090609270827 8 0.5655216155465134 1.86349750689325 9 0.35630879914251334 1.3208657427269261 >10 2.506997993980348 18.999612797539157 >50 0.1913268356991791 5.505156709022834 >100 0.11148313434204064 8.387273352349329 >500 0.005985851236849602 1.6891608818417294 >1k 0.0015401810192263797 1.0591661417891907 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.9263186409841794E-4 0.0 0.0 2 5.504266015184528E-4 0.0 0.0 3 7.66416786924428E-4 0.0 0.0 4 0.0016025078272056221 0.0 0.0 5 0.0017070192072407714 0.0 0.0 6 0.0021389995780527215 0.0 0.0 7 0.0026963936049068512 0.0 0.0 8 0.004027171844021085 0.0 0.0 9 0.004417347662818975 0.0 0.0 10-11 0.005054867081033386 3.483712667838309E-6 0.0 12-13 0.005782963028611593 6.967425335676618E-6 3.483712667838309E-6 14-15 0.006775821138945511 6.967425335676618E-6 6.967425335676618E-6 16-17 0.007935897457335667 2.0902276007029854E-5 6.967425335676618E-6 18-19 0.010503393693532502 2.0902276007029854E-5 6.967425335676618E-6 20-21 0.013300814965806663 2.0902276007029854E-5 6.967425335676618E-6 22-23 0.016718337092956045 2.0902276007029854E-5 6.967425335676618E-6 24-25 0.021724432196639692 2.0902276007029854E-5 6.967425335676618E-6 26-27 0.02997386379408081 2.0902276007029854E-5 6.967425335676618E-6 28-29 0.048089169666840015 2.0902276007029854E-5 6.967425335676618E-6 30-31 0.07931020259600693 3.83208393462214E-5 6.967425335676618E-6 32-33 0.12454272787521954 5.2255690017574634E-5 6.967425335676618E-6 34-35 0.181111254175578 6.967425335676617E-5 6.967425335676618E-6 36-37 0.25957839830596807 6.967425335676617E-5 6.967425335676618E-6 38-39 0.3621040621204495 6.967425335676617E-5 6.967425335676618E-6 40-41 0.4955407084366603 7.315796602460448E-5 6.967425335676618E-6 42-43 0.66627049886208 7.66416786924428E-5 6.967425335676618E-6 44-45 0.8728093718002099 8.01253913602811E-5 6.967425335676618E-6 46-47 1.1149448207783115 8.360910402811942E-5 6.967425335676618E-6 48-49 1.4045841756950566 8.360910402811942E-5 6.967425335676618E-6 50-51 1.7320218130578469 8.709281669595773E-5 6.967425335676618E-6 52-53 2.1139516839709644 1.1496251803866419E-4 6.967425335676618E-6 54-55 2.5473150887120464 1.3934850671353235E-4 6.967425335676618E-6 56-57 3.0341709014677853 1.4631593204920897E-4 6.967425335676618E-6 58-59 3.589157682868439 1.532833573848856E-4 6.967425335676618E-6 60-61 4.221162383929661 1.7418563339191545E-4 1.3934850671353236E-5 62-63 4.9334945316859 1.7418563339191545E-4 1.3934850671353236E-5 64-65 5.728003977563775 1.7418563339191545E-4 1.7418563339191547E-5 66-67 6.608467066095226 1.7766934605975375E-4 2.0902276007029854E-5 68-69 7.585408093249791 1.881204840632687E-4 2.0902276007029854E-5 70-71 8.654949686828166 1.985716220667836E-4 2.0902276007029854E-5 72-73 9.829800430642624 2.0553904740246024E-4 2.0902276007029854E-5 74-75 11.094816625726075 2.0902276007029853E-4 2.0902276007029854E-5 76-77 12.433802491885736 2.1599018540597516E-4 2.0902276007029854E-5 78-79 13.855171195214439 2.1599018540597516E-4 2.0902276007029854E-5 80-81 15.3426294115647 2.1599018540597516E-4 2.438598867486816E-5 82-83 16.898026992363146 2.2295761074165178E-4 2.7869701342706472E-5 84-85 18.519183133479707 2.299250360773284E-4 2.7869701342706472E-5 86-87 20.18116838706333 2.299250360773284E-4 2.7869701342706472E-5 88-89 21.885881376517993 2.299250360773284E-4 3.483712667838309E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCCC 515 0.0 19.835981 44-45 GGGGCGC 1440 0.0 17.652653 42-43 TCGGGGG 2430 0.0 16.128878 38-39 GGGGGGC 1945 0.0 13.067331 40-41 GGGGGCG 2095 0.0 12.471865 40-41 GGGCCCG 455 0.0 12.009057 44-45 CGAAAAT 8260 0.0 11.84298 1 GGGGCCC 735 0.0 10.9896555 44-45 CGGCAAC 3925 0.0 10.888717 1 CGGATAT 5460 0.0 10.436682 1 GGGCGCC 2705 0.0 10.275603 42-43 CAAAAAT 11785 0.0 10.275061 1 CGGGGGG 2490 0.0 10.207302 38-39 CTCGGGG 2605 0.0 9.939216 36-37 CGGGAAT 4405 0.0 9.917809 1 CCGAAAT 4800 0.0 9.299518 1 CGGAAAT 6815 0.0 9.197756 1 CACGAAC 2755 0.0 9.135411 1 AGGGTAG 2680 0.0 9.127177 28-29 GCCGTAT 22345 0.0 9.042184 94-95 >>END_MODULE