FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n02 tsrexp_lib#7.3420000000d07e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n02 tsrexp_lib#7.3420000000d07e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14352504
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC5150.019.83598144-45
GGGGCGC14400.017.65265342-43
TCGGGGG24300.016.12887838-39
GGGGGGC19450.013.06733140-41
GGGGGCG20950.012.47186540-41
GGGCCCG4550.012.00905744-45
CGAAAAT82600.011.842981
GGGGCCC7350.010.989655544-45
CGGCAAC39250.010.8887171
CGGATAT54600.010.4366821
GGGCGCC27050.010.27560342-43
CAAAAAT117850.010.2750611
CGGGGGG24900.010.20730238-39
CTCGGGG26050.09.93921636-37
CGGGAAT44050.09.9178091
CCGAAAT48000.09.2995181
CGGAAAT68150.09.1977561
CACGAAC27550.09.1354111
AGGGTAG26800.09.12717728-29
GCCGTAT223450.09.04218494-95
Produced by FastQC (version 0.11.2)