##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l02n02 tsrexp_lib#4.3420000000d061.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12330407 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.586674632881135 33.0 33.0 33.0 33.0 33.0 2 32.54245678995024 33.0 33.0 33.0 33.0 33.0 3 32.53083600565659 33.0 33.0 33.0 33.0 33.0 4 36.41486813857807 37.0 37.0 37.0 37.0 37.0 5 36.40130938094744 37.0 37.0 37.0 37.0 37.0 6 36.41611757016617 37.0 37.0 37.0 37.0 37.0 7 36.43944534839766 37.0 37.0 37.0 37.0 37.0 8 36.4472448476356 37.0 37.0 37.0 37.0 37.0 9 36.41812893929617 37.0 37.0 37.0 37.0 37.0 10-11 36.40207115628868 37.0 37.0 37.0 37.0 37.0 12-13 36.402668257422484 37.0 37.0 37.0 37.0 37.0 14-15 38.76678523263668 40.0 40.0 40.0 37.0 40.0 16-17 38.74153468737893 40.0 40.0 40.0 37.0 40.0 18-19 38.74471909159203 40.0 40.0 40.0 37.0 40.0 20-21 38.69333640000691 40.0 40.0 40.0 37.0 40.0 22-23 38.71041661479625 40.0 40.0 40.0 37.0 40.0 24-25 38.68347123497221 40.0 40.0 40.0 37.0 40.0 26-27 38.66248170072569 40.0 40.0 40.0 37.0 40.0 28-29 38.61830246154892 40.0 40.0 40.0 37.0 40.0 30-31 38.59821885846915 40.0 40.0 40.0 37.0 40.0 32-33 38.520273053436114 40.0 40.0 40.0 37.0 40.0 34-35 38.50641519781139 40.0 40.0 40.0 37.0 40.0 36-37 38.469215979650954 40.0 40.0 40.0 37.0 40.0 38-39 38.44532682497828 40.0 40.0 40.0 37.0 40.0 40-41 38.395859925791584 40.0 38.5 40.0 37.0 40.0 42-43 38.339030495911445 40.0 37.0 40.0 37.0 40.0 44-45 38.28654731348284 40.0 37.0 40.0 37.0 40.0 46-47 38.19653146891258 40.0 37.0 40.0 37.0 40.0 48-49 38.08878770181714 40.0 37.0 40.0 37.0 40.0 50-51 36.717573191217454 38.5 37.0 38.5 35.0 40.0 52-53 37.040420969072635 38.5 37.0 40.0 33.0 40.0 54-55 37.69140268443694 40.0 37.0 40.0 33.0 40.0 56-57 37.64055845845154 40.0 37.0 40.0 33.0 40.0 58-59 37.440366810276416 40.0 37.0 40.0 33.0 40.0 60-61 37.24969009538776 37.0 37.0 40.0 33.0 40.0 62-63 37.02616592461222 37.0 37.0 40.0 33.0 40.0 64-65 36.75116664843261 37.0 37.0 40.0 33.0 40.0 66-67 36.512611465298754 37.0 37.0 40.0 33.0 40.0 68-69 36.24814841878293 37.0 37.0 37.0 33.0 40.0 70-71 36.00310602075017 37.0 37.0 37.0 33.0 40.0 72-73 35.723498908024695 37.0 37.0 37.0 33.0 40.0 74-75 35.57506933874932 37.0 37.0 37.0 33.0 37.0 76-77 35.366257496609805 37.0 37.0 37.0 33.0 37.0 78-79 35.27171394261357 37.0 37.0 37.0 33.0 37.0 80-81 35.176442594311766 37.0 37.0 37.0 33.0 37.0 82-83 35.10043966918529 37.0 37.0 37.0 33.0 37.0 84-85 35.05416191858063 37.0 37.0 37.0 33.0 37.0 86-87 34.998984502295826 37.0 33.0 37.0 33.0 37.0 88-89 34.92277501464469 37.0 33.0 37.0 33.0 37.0 90-91 34.865512144084136 37.0 33.0 37.0 33.0 37.0 92-93 34.84357775051545 37.0 33.0 37.0 33.0 37.0 94-95 34.787849379181075 37.0 33.0 37.0 33.0 37.0 96-97 34.72184154180799 37.0 33.0 37.0 33.0 37.0 98-99 34.6653636007311 37.0 33.0 37.0 33.0 37.0 100-101 32.897261339386446 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17959717914396833 2306 2 0.24283905663106964 2306 3 0.22219177248766897 2306 4 0.26606918050242 2306 5 0.2972128834299639 2306 6 0.3058221589847676 2306 7 0.3067054582788842 2306 8 0.30565530806926944 2306 9 0.2933318460888259 2306 10-11 0.2979682450532266 2306 12-13 0.3064837737786732 2306 14-15 0.5413124432272767 2306 16-17 0.5715126558128603 2306 18-19 0.5419543126528552 2306 20-21 0.6140166826823048 2306 22-23 0.612982710012048 2306 24-25 0.5620562132423075 2306 26-27 0.5395238620468703 2306 28-29 0.6032204549697866 2306 30-31 0.6549592228135594 2306 32-33 0.7243849817826202 2306 34-35 0.7071559382752284 2306 36-37 0.6704958207378979 2306 38-39 0.6190552852858175 2306 40-41 0.6743162994365122 2306 42-43 0.6296521850444492 2306 44-45 0.7052870764365764 2306 46-47 0.6405447773091382 2306 48-49 0.693494264674186 2306 50-51 0.6600802872963953 2306 52-53 0.7437759947387903 2306 54-55 0.778754717882201 2306 56-57 0.7144657068913816 2306 58-59 0.740994592176186 2306 60-61 0.736822214612701 2306 62-63 0.6501264586090301 2306 64-65 0.7135867482695275 2306 66-67 0.6972769097009177 2306 68-69 0.721791817491038 2306 70-71 0.7130792206866516 2306 72-73 0.779860417684425 2306 74-75 0.7560984954045011 2306 76-77 0.7677321417941272 2306 78-79 0.7977621758731175 2306 80-81 0.7576377052440151 2306 82-83 0.763696708167295 2306 84-85 0.7602568346975644 2306 86-87 0.7387480243663447 2306 88-89 0.8649623514841593 2306 90-91 0.8881488899731025 2306 92-93 0.8519984983911755 2306 94-95 0.8493380773270545 2306 96-97 0.8804541078079566 2306 98-99 0.78468284151689 2306 100-101 0.8821486055828345 2307 1 0.1674149965557703 2307 2 0.23340447240522622 2307 3 0.24803011640874928 2307 4 0.2843129992752722 2307 5 0.2583419895205097 2307 6 0.29502935620394766 2307 7 0.2811849720088304 2307 8 0.2786730757775828 2307 9 0.3176278367816536 2307 10-11 0.3033626827696736 2307 12-13 0.30053551562185987 2307 14-15 0.5535315815156352 2307 16-17 0.5085275237219875 2307 18-19 0.5099511269942454 2307 20-21 0.5847051145824196 2307 22-23 0.5694797667544975 2307 24-25 0.6001730563499095 2307 26-27 0.581065191589353 2307 28-29 0.5947799082572018 2307 30-31 0.5375497401354181 2307 32-33 0.639683321802103 2307 34-35 0.6154703591748998 2307 36-37 0.6348260373021617 2307 38-39 0.5707775239256492 2307 40-41 0.6388644516193125 2307 42-43 0.5985815191787083 2307 44-45 0.6080962569020727 2307 46-47 0.6563786208757918 2307 48-49 0.6039881061419621 2307 50-51 0.5710148930904566 2307 52-53 0.653402568333739 2307 54-55 0.6259803041032654 2307 56-57 0.588433120204833 2307 58-59 0.5870131627374278 2307 60-61 0.601530003831293 2307 62-63 0.6132484684325519 2307 64-65 0.625285899254159 2307 66-67 0.5878199524684504 2307 68-69 0.5899493311836395 2307 70-71 0.5514203411139462 2307 72-73 0.6910567393689817 2307 74-75 0.6273572941124712 2307 76-77 0.6892393291627457 2307 78-79 0.7122325214990468 2307 80-81 0.7176064409792176 2307 82-83 0.7202037764278231 2307 84-85 0.709681510417731 2307 86-87 0.8000131712137488 2307 88-89 0.7866971962669922 2307 90-91 0.825160249022197 2307 92-93 0.7980899180373129 2307 94-95 0.7672365470133968 2307 96-97 0.7657033431018121 2307 98-99 0.8284726099385651 2307 100-101 0.802755818640172 2201 1 0.1734213959766322 2201 2 0.199459257022518 2201 3 0.23906219887740576 2201 4 0.23903609062944753 2201 5 0.25287965765251386 2201 6 0.2700005217475834 2201 7 0.21206895691562266 2201 8 0.21923571734219394 2201 9 0.2625038180536947 2201 10-11 0.2536581355469849 2201 12-13 0.2719502031047085 2201 14-15 0.48246966209399744 2201 16-17 0.45836197060235406 2201 18-19 0.5062118584642477 2201 20-21 0.5484978671431691 2201 22-23 0.5415193903991238 2201 24-25 0.5046753435060651 2201 26-27 0.5468980398480952 2201 28-29 0.5856660982727746 2201 30-31 0.5565279578603537 2201 32-33 0.6266742491117512 2201 34-35 0.6007523820063838 2201 36-37 0.5960857657272811 2201 38-39 0.6243912597028398 2201 40-41 0.5995947784935112 2201 42-43 0.5702520530096677 2201 44-45 0.60233542392141 2201 46-47 0.6371120037610183 2201 48-49 0.6066801261866246 2201 50-51 0.47558893400383084 2201 52-53 0.5686217965533444 2201 54-55 0.6336441947561795 2201 56-57 0.6962780167349507 2201 58-59 0.737205153038893 2201 60-61 0.714328787984094 2201 62-63 0.6855686485825956 2201 64-65 0.6633464496113746 2201 66-67 0.6645451279848515 2201 68-69 0.6494129102374728 2201 70-71 0.6443592889327547 2201 72-73 0.7625015236808963 2201 74-75 0.7058114404872882 2201 76-77 0.736919214255856 2201 78-79 0.740082409404188 2201 80-81 0.74993594267562 2201 82-83 0.719215096004497 2201 84-85 0.7311964667517046 2201 86-87 0.7719074643735837 2201 88-89 0.7945792265658085 2201 90-91 0.7473300793510802 2201 92-93 0.7275936950947823 2201 94-95 0.7251373635012541 2201 96-97 0.6889094840940047 2201 98-99 0.7190067364092769 2201 100-101 0.7628519163158458 1101 1 0.1726984228608046 1101 2 0.23463008660861107 1101 3 0.22026809506877498 1101 4 0.3142594732236432 1101 5 0.31202498274646473 1101 6 0.3561385018084309 1101 7 0.29461737883372763 1101 8 0.3394265867478765 1101 9 0.32839046780235037 1101 10-11 0.3182237438712221 1101 12-13 0.3314018292347072 1101 14-15 0.5519166898808194 1101 16-17 0.5365664971525703 1101 18-19 0.5319508384210039 1101 20-21 0.5991090023296124 1101 22-23 0.5891130978806913 1101 24-25 0.6155456349466348 1101 26-27 0.6019082559242932 1101 28-29 0.6423871443308897 1101 30-31 0.6047491163947143 1101 32-33 0.6907359490018905 1101 34-35 0.6578259309328089 1101 36-37 0.6684398501752682 1101 38-39 0.6511899998158057 1101 40-41 0.6760420593321896 1101 42-43 0.6617215263760983 1101 44-45 0.6582736731462049 1101 46-47 0.6955463227341951 1101 48-49 0.6462237047702573 1101 50-51 0.6160144947577422 1101 52-53 0.6124211447877883 1101 54-55 0.7051248912051236 1101 56-57 0.6880174691121965 1101 58-59 0.7042913685173531 1101 60-61 0.7176792764119924 1101 62-63 0.6717212817391598 1101 64-65 0.7094288145543786 1101 66-67 0.7004475428704211 1101 68-69 0.7204258018770915 1101 70-71 0.703973150590194 1101 72-73 0.7919562837451153 1101 74-75 0.736708566816624 1101 76-77 0.7643548394214648 1101 78-79 0.7487615992140846 1101 80-81 0.7955638770238949 1101 82-83 0.7934482688615176 1101 84-85 0.7685375103272563 1101 86-87 0.8299643783213 1101 88-89 0.8477784411436673 1101 90-91 0.8291479854565722 1101 92-93 0.7870636375944429 1101 94-95 0.8133735641994022 1101 96-97 0.8182098597901089 1101 98-99 0.7996032660077361 1101 100-101 0.8372353014164773 2304 1 0.16878635630003203 2304 2 0.18785610259295993 2304 3 0.2097205544762133 2304 4 0.2695889372706546 2304 5 0.2991283187382976 2304 6 0.25989465113333665 2304 7 0.24795814540169658 2304 8 0.2746248553711652 2304 9 0.27989960833235017 2304 10-11 0.2882888768384362 2304 12-13 0.2617518330619575 2304 14-15 0.42381266027481246 2304 16-17 0.4494576447106553 2304 18-19 0.4735314407143747 2304 20-21 0.4988841773204271 2304 22-23 0.47095038316095383 2304 24-25 0.47091986607705394 2304 26-27 0.5076402208028483 2304 28-29 0.5024927458763244 2304 30-31 0.4915936328758619 2304 32-33 0.5910588833674453 2304 34-35 0.5633645616545735 2304 36-37 0.5756736599733188 2304 38-39 0.558700014938573 2304 40-41 0.5727818660493256 2304 42-43 0.5778056113161796 2304 44-45 0.5950011870994061 2304 46-47 0.5764166022622064 2304 48-49 0.5753751727269147 2304 50-51 0.6265549627330174 2304 52-53 0.6139698757047185 2304 54-55 0.5966336600159678 2304 56-57 0.6128033329428249 2304 58-59 0.632299110994758 2304 60-61 0.6524321998386284 2304 62-63 0.6021733839172185 2304 64-65 0.6703608825342684 2304 66-67 0.6055419194352822 2304 68-69 0.5532970415966503 2304 70-71 0.6222459856740059 2304 72-73 0.6663710055233807 2304 74-75 0.5988202172012436 2304 76-77 0.6808011495566362 2304 78-79 0.6754428861961443 2304 80-81 0.677944710648319 2304 82-83 0.7083540117367946 2304 84-85 0.7258405887073778 2304 86-87 0.7648421109523653 2304 88-89 0.7469011265148069 2304 90-91 0.7806579299105891 2304 92-93 0.6979433576788168 2304 94-95 0.6809358960177789 2304 96-97 0.700107707346703 2304 98-99 0.7032378218243096 2304 100-101 0.8068525242865903 2202 1 0.13624403674626961 2202 2 0.17261144393391703 2202 3 0.1636777133022349 2202 4 0.22707520747016474 2202 5 0.21205035951188478 2202 6 0.22915402865427836 2202 7 0.21261237200712202 2202 8 0.21772961884615682 2202 9 0.2056340854944736 2202 10-11 0.25991400829591527 2202 12-13 0.27021136838988014 2202 14-15 0.3912040878435974 2202 16-17 0.3815663779221623 2202 18-19 0.41082660165060503 2202 20-21 0.42907382280530015 2202 22-23 0.4043124020428337 2202 24-25 0.40724926805827977 2202 26-27 0.40968897523051595 2202 28-29 0.46977694239640044 2202 30-31 0.4744675416590667 2202 32-33 0.5366595199386879 2202 34-35 0.519170519663561 2202 36-37 0.4953629577610954 2202 38-39 0.5512913438879323 2202 40-41 0.5355965000942859 2202 42-43 0.4811514636672882 2202 44-45 0.558892863387868 2202 46-47 0.5381983212147006 2202 48-49 0.5252665677286004 2202 50-51 0.4767439396397606 2202 52-53 0.5711580618197658 2202 54-55 0.5641589523730559 2202 56-57 0.5456174790616473 2202 58-59 0.5064525533550537 2202 60-61 0.5231567761110796 2202 62-63 0.5125328180833364 2202 64-65 0.5494362631771494 2202 66-67 0.5352549336815002 2202 68-69 0.5172413721419389 2202 70-71 0.5163733571333964 2202 72-73 0.6082900040841608 2202 74-75 0.5181108790077431 2202 76-77 0.6189629014448101 2202 78-79 0.5954511590632592 2202 80-81 0.6524944073667598 2202 82-83 0.6716841917812033 2202 84-85 0.673451741147467 2202 86-87 0.6061260713320991 2202 88-89 0.6453478292528771 2202 90-91 0.6779458329456176 2202 92-93 0.6473162317238348 2202 94-95 0.6590711288554942 2202 96-97 0.6798471299318649 2202 98-99 0.6465488434756637 2202 100-101 0.7456093753479038 1102 1 0.036295796471051744 1102 2 0.10468325747712015 1102 3 0.09782591448040989 1102 4 0.14974737333033517 1102 5 0.1208367744290868 1102 6 0.10626952089238983 1102 7 0.1071141513862841 1102 8 0.12744690731003772 1102 9 0.13657676779955352 1102 10-11 0.10383424186192514 1102 12-13 0.11177504237862479 1102 14-15 0.1618228751517492 1102 16-17 0.1912024477762131 1102 18-19 0.22385535813985769 1102 20-21 0.26310956453858836 1102 22-23 0.20004572059631442 1102 24-25 0.21411929529615037 1102 26-27 0.2385108748971021 1102 28-29 0.2284797014397597 1102 30-31 0.26713991369111056 1102 32-33 0.30050940504197854 1102 34-35 0.22568087024747996 1102 36-37 0.22385865219684575 1102 38-39 0.24831996870946682 1102 40-41 0.2581013768907212 1102 42-43 0.27519114837454595 1102 44-45 0.27882638883910005 1102 46-47 0.26950304434503636 1102 48-49 0.31190343474694515 1102 50-51 0.28632831530043035 1102 52-53 0.31562285124368117 1102 54-55 0.31149705427518626 1102 56-57 0.3454296358220432 1102 58-59 0.3433840268669073 1102 60-61 0.31731852565430074 1102 62-63 0.31776569450149594 1102 64-65 0.3417144260275151 1102 66-67 0.31446048945380767 1102 68-69 0.32936779905038804 1102 70-71 0.36014530070097805 1102 72-73 0.43052640125244324 1102 74-75 0.41379770555288076 1102 76-77 0.43883599686285635 1102 78-79 0.3699080196917919 1102 80-81 0.385721053059342 1102 82-83 0.39538172746784284 1102 84-85 0.3828547611771924 1102 86-87 0.37571524691583136 1102 88-89 0.3797624962288495 1102 90-91 0.3897693308726602 1102 92-93 0.3784280026857445 1102 94-95 0.40500873650972835 1102 96-97 0.3745212493335046 1102 98-99 0.43575804481020697 1102 100-101 0.4574339948700654 2305 1 0.12217956643340955 2305 2 0.1251465116454753 2305 3 0.15122144853694408 2305 4 0.15149345005339399 2305 5 0.15795409336877242 2305 6 0.16642777712657164 2305 7 0.16359755584370106 2305 8 0.16475222395639122 2305 9 0.18625837629114983 2305 10-11 0.17396706900385794 2305 12-13 0.17899032876918852 2305 14-15 0.3024277363516248 2305 16-17 0.2849867336458445 2305 18-19 0.329861837496388 2305 20-21 0.3585969542933469 2305 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-1.0954712536535425 1213 82-83 -1.1423373380726574 1213 84-85 -1.089149529337071 1213 86-87 -1.1213490612672317 1213 88-89 -1.1888217895737867 1213 90-91 -1.301149059289422 1213 92-93 -1.2128810904772962 1213 94-95 -1.1682517265209 1213 96-97 -1.1879313410755756 1213 98-99 -1.3304282866202115 1213 100-101 -1.3870267329886552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16457.0 3 1198.0 4 864.0 5 709.0 6 681.0 7 1715.0 8 2882.0 9 4559.0 10 8509.0 11 13182.0 12 12634.0 13 11452.0 14 11165.0 15 11438.0 16 12095.0 17 13439.0 18 15147.0 19 17349.0 20 19010.0 21 21300.0 22 24400.0 23 27287.0 24 31466.0 25 35526.0 26 41655.0 27 49804.0 28 60078.0 29 73513.0 30 93609.0 31 121347.0 32 161798.0 33 224106.0 34 319174.0 35 480898.0 36 989576.0 37 4596071.0 38 4801800.0 39 2514.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.820559163082315 14.752621379034766 20.39088012975236 41.03593932813055 2 26.011276981132937 24.963046164050095 30.246752290025093 18.778924564791875 3 23.42066956486918 26.86312235360188 30.009416545389282 19.706791536139658 4 25.21702928298367 30.33098290527138 22.08999806619431 22.36198974555064 5 28.131519047418653 33.35386553159518 21.55429119964852 16.96032422133764 6 19.45904201102001 37.57617103150581 22.18338261881901 20.781404338655168 7 18.483626397905013 16.162299939567614 43.12560513610283 22.228468526424546 8 22.37008960513802 21.639238815194783 28.566431493448746 27.42424008621845 9 24.48097972272017 22.78266553431098 27.09198353589058 25.644371207078276 10-11 24.392524263924216 31.061952785359093 22.082157761708647 22.463365189008037 12-13 22.96701092971324 25.14934394638158 27.948668194080927 23.934976929824252 14-15 23.252771497282346 26.648695902087947 27.87932857340599 22.21920402722372 16-17 24.113705824656524 26.638480747680564 26.952125787942098 22.295687639720814 18-19 23.886140881399136 26.880732159706934 26.8578726843 22.375254274593935 20-21 23.863954546396627 27.228837078124297 26.713891576689136 22.19331679878994 22-23 24.04309248561607 27.008176393513576 26.80343032353902 22.145300797331334 24-25 23.541074857496536 27.412519502836822 26.867585803746834 22.178819835919803 26-27 23.92547600575004 27.548669150843143 26.48420837460414 22.041646468802682 28-29 24.047730760242207 26.920798528485886 26.809956350585185 22.22151436068673 30-31 23.619747697653988 27.083282742213832 26.963723913560617 22.333245646571562 32-33 23.774483979037704 27.240705148019877 26.82698907488662 22.1578217980558 34-35 24.090328250780953 26.823054206065773 26.89395776560725 22.192659777546023 36-37 23.60807580986536 26.99881315032848 27.116929411062664 22.276181628743494 38-39 23.98940582435929 27.10001096789656 26.87873568587944 22.031847521864705 40-41 24.20129620927382 26.626686235074793 26.87283417204519 22.2991833836062 42-43 23.837514455268284 26.74875734058656 27.144387398508396 22.269340805636766 44-45 24.195354902061865 26.85277412535803 26.86817867703744 22.08369229554266 46-47 24.112846690704018 26.759791093439517 26.882687581373066 22.244674634483395 48-49 23.880921182232175 26.695599059815084 27.056502092851492 22.366977665101253 50-51 24.161292316216475 26.93967157446286 26.6305726917104 22.268463417610263 52-53 24.23186387601664 26.737418721430416 26.901646284649928 22.129071117903017 54-55 23.77751133300673 26.864114669425387 27.06536562234585 22.293008375222033 56-57 24.120429367271036 26.917760784955696 27.041031461313075 21.920778386460192 58-59 24.406958389555715 26.921641737242414 26.848701740767154 21.822698132434713 60-61 23.84451316034499 27.022206806610725 26.822316507459636 22.310963525584647 62-63 24.46443654468965 27.058218247247762 26.581670459856614 21.895674748205977 64-65 24.454954965750133 26.99162502154338 26.746667168177783 21.806752844528695 66-67 24.249269522867596 27.113687962656503 26.83172939516672 21.80531311930918 68-69 24.66680381837506 27.107567971143542 26.61368775674812 21.61194045373328 70-71 24.699095260999652 27.11778325175575 26.52725325892637 21.655868228318226 72-73 24.606909721955645 27.14483661363692 26.494913759377354 21.753339905030085 74-75 24.87312266341815 27.20195239225468 26.35385772728542 21.571067217041747 76-77 25.130007874042633 27.101152426214792 26.42290031084828 21.3459393888943 78-79 24.974704358587957 27.094470574762326 26.5191263008592 21.411698765790526 80-81 25.350347600058555 27.254441601447176 26.043816918379388 21.35139388011488 82-83 25.569077845785237 27.191995017193886 26.066237447282596 21.172689689738274 84-85 25.40304714249759 27.187989932006435 26.222391780524106 21.18657114497186 86-87 25.694266073416134 27.36896898106833 25.94237171217179 20.994393233343747 88-89 25.97673899459661 27.245333160466362 25.882173938614677 20.89575390632235 90-91 25.959724990628448 27.216634225739135 25.948976343714918 20.874664439917503 92-93 26.435493676995886 27.426321514338355 25.423257398147747 20.714927410518015 94-95 26.62315116695545 27.357829067741356 25.529411301236742 20.48960846406645 96-97 26.50576449435723 27.568316379193014 25.57502887923312 20.350890247216633 98-99 27.15017870512345 27.56565263362881 25.217442334828483 20.06672632641926 100-101 27.389576227549284 27.47551781634932 25.034435527440507 20.100470428660884 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14107.0 1 7728.5 2 1083.5 3 700.5 4 508.0 5 430.0 6 431.5 7 414.0 8 416.0 9 445.0 10 457.5 11 470.0 12 501.0 13 536.5 14 569.0 15 626.0 16 674.5 17 748.0 18 870.0 19 1014.0 20 1242.5 21 1552.0 22 1936.5 23 2647.5 24 3838.0 25 5461.0 26 8055.5 27 11976.5 28 17596.5 29 25168.5 30 35120.0 31 47431.5 32 61387.5 33 77627.0 34 97031.5 35 120755.5 36 153159.0 37 194212.5 38 242750.0 39 303074.5 40 377993.0 41 462940.0 42 550347.5 43 637953.5 44 715816.0 45 777168.0 46 815123.5 47 822072.5 48 805558.5 49 768144.5 50 705500.0 51 629314.5 52 544196.0 53 459374.0 54 380064.0 55 310821.5 56 255707.0 57 211469.5 58 176108.5 59 142993.5 60 113278.5 61 81152.0 62 51239.0 63 32823.5 64 21379.5 65 13177.0 66 8888.5 67 5658.5 68 3781.0 69 2957.5 70 2666.0 71 2118.0 72 1229.5 73 888.5 74 857.0 75 705.5 76 504.5 77 234.0 78 109.0 79 77.5 80 60.5 81 42.5 82 34.0 83 33.0 84 24.0 85 16.0 86 15.5 87 14.0 88 14.5 89 14.5 90 8.5 91 5.5 92 7.5 93 7.0 94 4.0 95 2.0 96 1.0 97 3.0 98 2.5 99 0.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16372533364064948 2 0.14941923652641798 3 0.15488539834897583 4 0.1451533594957571 5 0.17262203915896696 6 0.15250104882993726 7 0.1551205892879286 8 0.14190123651230654 9 0.1700268287981086 10-11 0.14132136919730226 12-13 0.1308269873005814 14-15 0.191125078028649 16-17 0.1996446670414042 18-19 0.18329078675180793 20-21 0.21793279005307772 22-23 0.2021060618680308 24-25 0.19353781266100947 26-27 0.17816524628911276 28-29 0.19602759260095795 30-31 0.14306097114231509 32-33 0.16932125598125025 34-35 0.17383043398324158 36-37 0.17656756991070935 38-39 0.17644997444123295 40-41 0.2126531589752066 42-43 0.19739818807278622 44-45 0.18131193885165348 46-47 0.18567919128703536 48-49 0.22654158942198746 50-51 0.20373617837594493 52-53 0.16754110387434898 54-55 0.15338098734291578 56-57 0.1498450132262463 58-59 0.16454039189460656 60-61 0.159398631367156 62-63 0.1476877446137828 64-65 0.16984435306961074 66-67 0.17297888058358496 68-69 0.18546022041283794 70-71 0.16583394205884688 72-73 0.17486446311139608 74-75 0.20848865734926672 76-77 0.23704813636727484 78-79 0.2181923110891636 80-81 0.19423933046167902 82-83 0.16280484496578254 84-85 0.17275179967700985 86-87 0.16159239512531906 88-89 0.1651445893067439 90-91 0.17745561845606556 92-93 0.16092331745416028 94-95 0.15540038540495865 96-97 0.16293460548382546 98-99 0.15347830773144797 100-101 0.1599298384878942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2330407E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.07966525667491 #Duplication Level Percentage of deduplicated Percentage of total 1 70.28893671481741 30.28023864921941 2 14.308525415794785 12.32812970458129 3 5.610789664995405 7.251328217808398 4 2.7132521885819765 4.675439841642085 5 1.6614296152725603 3.578691583673404 6 1.0936571105356514 2.82686293364749 7 0.6756563028066199 2.0374933147430236 8 0.566304472679796 1.951696569312273 9 0.47167737568931806 1.8287733108458442 >10 2.3301249464870635 18.484455534216227 >50 0.1777047759644309 5.233627465338266 >100 0.09563126487163073 7.1841699791825935 >500 0.004924313309137908 1.3891609076560727 >1k 0.0013858381941043768 0.9499319881335699 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.162912627296082E-4 0.0 0.0 2 6.163624607038519E-4 0.0 0.0 3 8.596634320343198E-4 0.0 0.0 4 0.0018166472526008266 0.0 0.0 5 0.0019139676411330138 0.0 0.0 6 0.0023762394866609026 0.0 0.0 7 0.0030007119797424366 0.0 0.0 8 0.004379417483948421 0.0 0.0 9 0.004817359232343263 0.0 0.0 10-11 0.0055756472596565545 8.110032377682262E-6 0.0 12-13 0.0062163398174934536 8.110032377682262E-6 0.0 14-15 0.007274699042780988 8.110032377682262E-6 0.0 16-17 0.008637184482231608 1.2165048566523392E-5 0.0 18-19 0.010993148887948305 2.4330097133046783E-5 0.0 20-21 0.013341003261287319 2.4330097133046783E-5 0.0 22-23 0.015615867343227193 3.2440129510729047E-5 0.0 24-25 0.018815275116222847 3.2440129510729047E-5 4.055016188841131E-6 26-27 0.022833796159364407 3.2440129510729047E-5 8.110032377682262E-6 28-29 0.03128850491309816 3.6495145699570175E-5 8.110032377682262E-6 30-31 0.04699763762866871 4.8660194266093566E-5 8.110032377682262E-6 32-33 0.0671186279576984 4.8660194266093566E-5 8.110032377682262E-6 34-35 0.09169608107826449 9.3265372343346E-5 8.110032377682262E-6 36-37 0.1262732041205128 9.732038853218713E-5 8.110032377682262E-6 38-39 0.17220842750770515 1.054304209098694E-4 8.110032377682262E-6 40-41 0.23320803603644225 1.054304209098694E-4 8.110032377682262E-6 42-43 0.3105088096443207 1.0948543709871054E-4 8.110032377682262E-6 44-45 0.402873968393744 1.1354045328755166E-4 8.110032377682262E-6 46-47 0.5139814119679911 1.1759546947639278E-4 8.110032377682262E-6 48-49 0.6472454639980659 1.2165048566523392E-4 8.110032377682262E-6 50-51 0.8027999400182005 1.419255666094396E-4 8.110032377682262E-6 52-53 0.9831995002273648 1.5409061517596297E-4 8.110032377682262E-6 54-55 1.1869600087004426 1.6220064755364524E-4 8.110032377682262E-6 56-57 1.4189272097830996 1.6220064755364524E-4 8.110032377682262E-6 58-59 1.6803054432834212 1.6220064755364524E-4 8.110032377682262E-6 60-61 1.9763054050040685 1.6220064755364524E-4 8.110032377682262E-6 62-63 2.3142139590363886 1.6220064755364524E-4 8.110032377682262E-6 64-65 2.6884757332016695 1.6220064755364524E-4 8.110032377682262E-6 66-67 3.1017427080874134 1.6220064755364524E-4 8.110032377682262E-6 68-69 3.561800514776195 1.6220064755364524E-4 8.110032377682262E-6 70-71 4.071041612819431 1.6220064755364524E-4 8.110032377682262E-6 72-73 4.635779662423147 1.6220064755364524E-4 8.110032377682262E-6 74-75 5.257077077828818 1.7031067993132748E-4 8.110032377682262E-6 76-77 5.932760370359226 1.7031067993132748E-4 8.110032377682262E-6 78-79 6.669885268182956 1.7031067993132748E-4 8.110032377682262E-6 80-81 7.4739828133815855 1.7031067993132748E-4 8.110032377682262E-6 82-83 8.350093391077845 1.7031067993132748E-4 8.110032377682262E-6 84-85 9.3019354511169 1.824757284978509E-4 1.2165048566523392E-5 86-87 10.329508993498754 1.9464077706437427E-4 1.6220064755364523E-5 88-89 11.439375034416951 2.0275080944205654E-4 1.6220064755364523E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCCC 595 0.0 24.356092 44-45 GGGGCGC 1540 0.0 22.67698 42-43 TCGGGGG 2525 0.0 22.201971 38-39 GGGGCCC 670 0.0 17.374647 44-45 GGGGGGC 2145 0.0 16.722294 40-41 GGGCGCC 2425 0.0 16.556309 42-43 GGGGGCG 2240 0.0 15.800995 40-41 CGGGGGG 2370 0.0 15.234778 38-39 GTAGGGG 1580 0.0 15.186577 18-19 AGGGTAG 2620 0.0 14.140262 28-29 CTCGGGG 2655 0.0 13.8679 36-37 GGGCCCG 450 0.0 13.726366 44-45 GGGCGGG 1715 0.0 13.29588 12-13 CGGGTAG 1360 0.0 12.57702 14-15 CGGGGGT 1105 0.0 12.468281 38-39 GGGTAGG 1505 0.0 11.997172 16-17 GGGTAGA 3270 0.0 11.910506 28-29 GCGCCCG 960 0.0 11.878586 44-45 AGGGGCG 1435 0.0 11.751697 10-11 AAGGGGC 2105 0.0 11.7346945 9 >>END_MODULE