FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n02 tsrexp_lib#4.3420000000d061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n02 tsrexp_lib#4.3420000000d061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12330407
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC5950.024.35609244-45
GGGGCGC15400.022.6769842-43
TCGGGGG25250.022.20197138-39
GGGGCCC6700.017.37464744-45
GGGGGGC21450.016.72229440-41
GGGCGCC24250.016.55630942-43
GGGGGCG22400.015.80099540-41
CGGGGGG23700.015.23477838-39
GTAGGGG15800.015.18657718-19
AGGGTAG26200.014.14026228-29
CTCGGGG26550.013.867936-37
GGGCCCG4500.013.72636644-45
GGGCGGG17150.013.2958812-13
CGGGTAG13600.012.5770214-15
CGGGGGT11050.012.46828138-39
GGGTAGG15050.011.99717216-17
GGGTAGA32700.011.91050628-29
GCGCCCG9600.011.87858644-45
AGGGGCG14350.011.75169710-11
AAGGGGC21050.011.73469459
Produced by FastQC (version 0.11.2)