##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l02n02 tsrexp_lib#38.3420000000d103.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6584223 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.60236188841113 33.0 33.0 33.0 33.0 33.0 2 32.56444670844229 33.0 33.0 33.0 33.0 33.0 3 32.55605771554214 33.0 33.0 33.0 33.0 33.0 4 36.44457546471315 37.0 37.0 37.0 37.0 37.0 5 36.42760170790084 37.0 37.0 37.0 37.0 37.0 6 36.43572400266516 37.0 37.0 37.0 37.0 37.0 7 36.475413727633466 37.0 37.0 37.0 37.0 37.0 8 36.474068390453965 37.0 37.0 37.0 37.0 37.0 9 36.455393901452 37.0 37.0 37.0 37.0 37.0 10-11 36.43482731371644 37.0 37.0 37.0 37.0 37.0 12-13 36.43674287763339 37.0 37.0 37.0 37.0 37.0 14-15 38.798124774935474 40.0 40.0 40.0 37.0 40.0 16-17 38.77653619872838 40.0 40.0 40.0 37.0 40.0 18-19 38.775681048469956 40.0 40.0 40.0 37.0 40.0 20-21 38.71717034796664 40.0 40.0 40.0 37.0 40.0 22-23 38.72380613475576 40.0 40.0 40.0 37.0 40.0 24-25 38.70778252802191 40.0 40.0 40.0 37.0 40.0 26-27 38.68502350239352 40.0 40.0 40.0 37.0 40.0 28-29 38.65001193003336 40.0 40.0 40.0 37.0 40.0 30-31 38.6349584453625 40.0 40.0 40.0 37.0 40.0 32-33 38.55188562112796 40.0 40.0 40.0 37.0 40.0 34-35 38.53757603592709 40.0 40.0 40.0 37.0 40.0 36-37 38.504495670939455 40.0 40.0 40.0 37.0 40.0 38-39 38.4800918802416 40.0 40.0 40.0 37.0 40.0 40-41 38.43126850351211 40.0 38.5 40.0 37.0 40.0 42-43 38.38640390825159 40.0 37.0 40.0 37.0 40.0 44-45 38.333967576736086 40.0 37.0 40.0 37.0 40.0 46-47 38.25611586970854 40.0 37.0 40.0 37.0 40.0 48-49 38.14915207155043 40.0 37.0 40.0 37.0 40.0 50-51 36.78451170320325 38.5 37.0 38.5 35.0 40.0 52-53 37.10282792669689 38.5 37.0 40.0 33.0 40.0 54-55 37.773745892263975 40.0 37.0 40.0 35.0 40.0 56-57 37.715637820893974 40.0 37.0 40.0 35.0 40.0 58-59 37.51341487066887 40.0 37.0 40.0 33.0 40.0 60-61 37.29895000518664 37.0 37.0 40.0 33.0 40.0 62-63 37.065951821498146 37.0 37.0 40.0 33.0 40.0 64-65 36.78343845583602 37.0 37.0 40.0 33.0 40.0 66-67 36.53843809664406 37.0 37.0 40.0 33.0 40.0 68-69 36.27305560580193 37.0 37.0 37.0 33.0 40.0 70-71 36.02075924524428 37.0 37.0 37.0 33.0 40.0 72-73 35.72327023249365 37.0 37.0 37.0 33.0 38.5 74-75 35.57279385889573 37.0 37.0 37.0 33.0 37.0 76-77 35.357957423374025 37.0 37.0 37.0 33.0 37.0 78-79 35.26083328283383 37.0 37.0 37.0 33.0 37.0 80-81 35.16126861134563 37.0 37.0 37.0 33.0 37.0 82-83 35.086823380678325 37.0 37.0 37.0 33.0 37.0 84-85 35.03673539003767 37.0 35.0 37.0 33.0 37.0 86-87 34.97340248044454 37.0 33.0 37.0 33.0 37.0 88-89 34.88809461647942 37.0 33.0 37.0 33.0 37.0 90-91 34.827324347914704 37.0 33.0 37.0 33.0 37.0 92-93 34.80020945523869 37.0 33.0 37.0 33.0 37.0 94-95 34.73765355456521 37.0 33.0 37.0 33.0 37.0 96-97 34.66077158990514 37.0 33.0 37.0 33.0 37.0 98-99 34.59643043681844 37.0 33.0 37.0 33.0 37.0 100-101 32.73346072877544 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.20414735633375614 2306 2 0.2161089229120492 2306 3 0.24235413226065106 2306 4 0.3198920265769729 2306 5 0.3390226119332951 2306 6 0.352729977339294 2306 7 0.3631882769468362 2306 8 0.3441842382574265 2306 9 0.3328346863808136 2306 10-11 0.34227962123733846 2306 12-13 0.31661394562847534 2306 14-15 0.5820181595306835 2306 16-17 0.6038506720496599 2306 18-19 0.566558690975377 2306 20-21 0.6223517497848192 2306 22-23 0.5967065319189118 2306 24-25 0.6063473549461733 2306 26-27 0.6173259884295916 2306 28-29 0.7345146346125659 2306 30-31 0.7094737696534992 2306 32-33 0.7164903983852682 2306 34-35 0.7433812446752839 2306 36-37 0.7405909548761329 2306 38-39 0.6793604410326282 2306 40-41 0.8006366325710133 2306 42-43 0.7249096483967676 2306 44-45 0.7395403093692039 2306 46-47 0.7315870834065663 2306 48-49 0.7349931833347014 2306 50-51 0.6694632107403677 2306 52-53 0.7711191893842226 2306 54-55 0.8002861805295396 2306 56-57 0.7737028651971016 2306 58-59 0.7704427077979901 2306 60-61 0.757624608965088 2306 62-63 0.7027078357792576 2306 64-65 0.6633339122369222 2306 66-67 0.6576482731021329 2306 68-69 0.6799095436993099 2306 70-71 0.7182355498982105 2306 72-73 0.8285238333947831 2306 74-75 0.8221903480802126 2306 76-77 0.8585605972769628 2306 78-79 0.8542255892420911 2306 80-81 0.8433871669739403 2306 82-83 0.8571245075532445 2306 84-85 0.8668959468569 2306 86-87 0.8191168342850759 2306 88-89 0.8733115711233594 2306 90-91 0.9416434848334987 2306 92-93 0.8895977312876369 2306 94-95 0.9037399619452628 2306 96-97 0.9285917845517844 2306 98-99 0.874264939352905 2306 100-101 1.0024022856315824 2307 1 0.1053806175966514 2307 2 0.17620810796171327 2307 3 0.2012538233153478 2307 4 0.21698249765555744 2307 5 0.2071085118309881 2307 6 0.24381289866155953 2307 7 0.23544216648251393 2307 8 0.24323522683199172 2307 9 0.26510863468161006 2307 10-11 0.29870472594107156 2307 12-13 0.2542805770159049 2307 14-15 0.4916554385944778 2307 16-17 0.4916475846125934 2307 18-19 0.5064831934121656 2307 20-21 0.5493968114478776 2307 22-23 0.5214130842808586 2307 24-25 0.5579316445987743 2307 26-27 0.5131413671521798 2307 28-29 0.5409903902181838 2307 30-31 0.5464862232235106 2307 32-33 0.6910439900633989 2307 34-35 0.5716872870002803 2307 36-37 0.5754967341188433 2307 38-39 0.5581334292342461 2307 40-41 0.6394231276797981 2307 42-43 0.5958808857403426 2307 44-45 0.5979748185090443 2307 46-47 0.6765741560868364 2307 48-49 0.6246674551964801 2307 50-51 0.5852390767195885 2307 52-53 0.6878828739475438 2307 54-55 0.6569970470379545 2307 56-57 0.5476310064350045 2307 58-59 0.5738491699458947 2307 60-61 0.5911948171629717 2307 62-63 0.6253177935953786 2307 64-65 0.6917387364203336 2307 66-67 0.6070628897932409 2307 68-69 0.6125262045701021 2307 70-71 0.6303549751490678 2307 72-73 0.6678949558288494 2307 74-75 0.6370096427978993 2307 76-77 0.7230454952443779 2307 78-79 0.776386996782783 2307 80-81 0.7521223055961386 2307 82-83 0.7612355464259224 2307 84-85 0.7133052767026271 2307 86-87 0.8213117067002429 2307 88-89 0.8291092094305625 2307 90-91 0.8624138825312784 2307 92-93 0.7771426493138165 2307 94-95 0.7786397941168985 2307 96-97 0.7476244279240802 2307 98-99 0.8730049666691073 2307 100-101 0.8277801514394554 2201 1 0.21436229839199683 2201 2 0.23346487536339566 2201 3 0.22708793793899673 2201 4 0.245141699637621 2201 5 0.27540275693782945 2201 6 0.24143446151831682 2201 7 0.2412792257178893 2201 8 0.22225831372908544 2201 9 0.27175357267248756 2201 10-11 0.26344292632093413 2201 12-13 0.286337647651834 2201 14-15 0.5337979896641087 2201 16-17 0.5425294973090757 2201 18-19 0.5377138240132879 2201 20-21 0.5654965539092061 2201 22-23 0.5639938445552986 2201 24-25 0.5057846380811313 2201 26-27 0.5839442747450931 2201 28-29 0.5963614279685743 2201 30-31 0.5533228499551512 2201 32-33 0.6557178743137868 2201 34-35 0.6346092458235901 2201 36-37 0.6782432699580099 2201 38-39 0.6129529635839859 2201 40-41 0.6374563730427951 2201 42-43 0.6373754962635658 2201 44-45 0.6506309449871566 2201 46-47 0.6713448027684734 2201 48-49 0.6583098665563725 2201 50-51 0.5588725386530768 2201 52-53 0.6455060654546756 2201 54-55 0.6932298127419259 2201 56-57 0.7085632628041338 2201 58-59 0.756687440740059 2201 60-61 0.7208128986498821 2201 62-63 0.6730853014572418 2201 64-65 0.6945542713145443 2201 66-67 0.7190401239581163 2201 68-69 0.7042260005886902 2201 70-71 0.6889976884539166 2201 72-73 0.7429219808292231 2201 74-75 0.6895313945054795 2201 76-77 0.8113171230063259 2201 78-79 0.7869351013575425 2201 80-81 0.7416415551247084 2201 82-83 0.7642499945109122 2201 84-85 0.7418808663455678 2201 86-87 0.8182209387841795 2201 88-89 0.8204960348642132 2201 90-91 0.7526272127604443 2201 92-93 0.812914606251347 2201 94-95 0.8426769486853729 2201 96-97 0.8426570294589055 2201 98-99 0.8383875879915195 2201 100-101 0.8516832261431588 1101 1 0.16221671199537013 1101 2 0.1917714512854971 1101 3 0.19013117736714236 1101 4 0.24025459138729843 1101 5 0.2889761754659048 1101 6 0.2554993694377856 1101 7 0.2128335916895665 1101 8 0.23300161618072934 1101 9 0.26273511403464767 1101 10-11 0.26979173718288507 1101 12-13 0.276589967678575 1101 14-15 0.5085422229354819 1101 16-17 0.48339139884411964 1101 18-19 0.49709270718926035 1101 20-21 0.5505033549987672 1101 22-23 0.5086074809424801 1101 24-25 0.5436768797937432 1101 26-27 0.49667989458261275 1101 28-29 0.5703177305035041 1101 30-31 0.5359904150249406 1101 32-33 0.6035547390769977 1101 34-35 0.57082061331635 1101 36-37 0.5741687461271923 1101 38-39 0.5945658921070134 1101 40-41 0.5858147064660031 1101 42-43 0.571298720453477 1101 44-45 0.5988822792321713 1101 46-47 0.5371082708482646 1101 48-49 0.5663441947687957 1101 50-51 0.5826668465039688 1101 52-53 0.5972018165818938 1101 54-55 0.6709976563325242 1101 56-57 0.6376074524288597 1101 58-59 0.6978900213213777 1101 60-61 0.6911120350824262 1101 62-63 0.6390986211487899 1101 64-65 0.6606415033085611 1101 66-67 0.6090244341982114 1101 68-69 0.6441184810386389 1101 70-71 0.6850986469313511 1101 72-73 0.7896330021893903 1101 74-75 0.6851352272452971 1101 76-77 0.7285161501912469 1101 78-79 0.7476380384301535 1101 80-81 0.7963117371652899 1101 82-83 0.7484408354665675 1101 84-85 0.7423604432430295 1101 86-87 0.7799333911669564 1101 88-89 0.7302397297883516 1101 90-91 0.7415540469539437 1101 92-93 0.7320766357284043 1101 94-95 0.743645736062355 1101 96-97 0.7866276958413749 1101 98-99 0.7301715378832441 1101 100-101 0.7928284073931238 2304 1 0.15405774192540633 2304 2 0.16796214780572427 2304 3 0.17599656066317948 2304 4 0.25308159662997554 2304 5 0.26643119245097324 2304 6 0.2504332591933789 2304 7 0.19432392519298247 2304 8 0.2594835270857274 2304 9 0.27815824948770995 2304 10-11 0.27382240018502557 2304 12-13 0.24572651864978923 2304 14-15 0.47277436670243134 2304 16-17 0.5125463958217296 2304 18-19 0.4861160197631236 2304 20-21 0.5253841000038406 2304 22-23 0.5296814210071048 2304 24-25 0.5189785951702248 2304 26-27 0.5577629321384592 2304 28-29 0.5312252161549864 2304 30-31 0.5196475320381637 2304 32-33 0.62414444754139 2304 34-35 0.6076786421421971 2304 36-37 0.6498923306685 2304 38-39 0.6156121769896288 2304 40-41 0.6268214646479251 2304 42-43 0.609895409298538 2304 44-45 0.6123551925237152 2304 46-47 0.6087953954358127 2304 48-49 0.6384058609318188 2304 50-51 0.664515708168814 2304 52-53 0.6833628390918349 2304 54-55 0.6674405351391215 2304 56-57 0.6500847525768236 2304 58-59 0.6353595806775374 2304 60-61 0.6872397777718078 2304 62-63 0.6311787088540086 2304 64-65 0.6976204437811049 2304 66-67 0.6347530870834532 2304 68-69 0.5837020131435935 2304 70-71 0.6003921008540445 2304 72-73 0.6622958335077769 2304 74-75 0.6302788868746703 2304 76-77 0.6871477207301027 2304 78-79 0.7100849604243464 2304 80-81 0.7225772781809567 2304 82-83 0.7206806967311579 2304 84-85 0.6852030853638098 2304 86-87 0.7243100183686124 2304 88-89 0.771058565690268 2304 90-91 0.8133881428789138 2304 92-93 0.7801070921676114 2304 94-95 0.7238314895378934 2304 96-97 0.8142017737032603 2304 98-99 0.758413062926131 2304 100-101 0.874584305100008 2202 1 0.12593456733351616 2202 2 0.20058571865713049 2202 3 0.18970538148901284 2202 4 0.24386986280971712 2202 5 0.24355819651152188 2202 6 0.24677757363982522 2202 7 0.2449033188279799 2202 8 0.22275771049895354 2202 9 0.24737282737535082 2202 10-11 0.28772660881855217 2202 12-13 0.2825422051185882 2202 14-15 0.4515384586875939 2202 16-17 0.4652357414044701 2202 18-19 0.46980257971045347 2202 20-21 0.5269404421322861 2202 22-23 0.4286467307598869 2202 24-25 0.4941791879616133 2202 26-27 0.45365087210140587 2202 28-29 0.48844067562787785 2202 30-31 0.48418093385099326 2202 32-33 0.5981667568295563 2202 34-35 0.6084150456941799 2202 36-37 0.5450493037109467 2202 38-39 0.5776918142802714 2202 40-41 0.5574023937767478 2202 42-43 0.4914492685410252 2202 44-45 0.5636430690624437 2202 46-47 0.5721878137648417 2202 48-49 0.548542077388035 2202 50-51 0.4925645096421363 2202 52-53 0.5938104563439026 2202 54-55 0.5613154559297584 2202 56-57 0.5303246016294523 2202 58-59 0.5214622458372062 2202 60-61 0.5418466185480924 2202 62-63 0.5148546035486348 2202 64-65 0.5469164992827729 2202 66-67 0.5431708079681172 2202 68-69 0.571145238164732 2202 70-71 0.5352877770963076 2202 72-73 0.6595303845394014 2202 74-75 0.6044406575789196 2202 76-77 0.6475413876879159 2202 78-79 0.6749298575831375 2202 80-81 0.6963029364908451 2202 82-83 0.7325979347483909 2202 84-85 0.7101231857911614 2202 86-87 0.6883060918915334 2202 88-89 0.708563777765228 2202 90-91 0.783820526216843 2202 92-93 0.773213538008072 2202 94-95 0.7176219581679604 2202 96-97 0.7541326689169665 2202 98-99 0.7144599775574036 2202 100-101 0.7916116174128334 1102 1 0.10908586503687445 1102 2 0.13503352689591708 1102 3 0.1537123811526655 1102 4 0.15558135508759108 1102 5 0.18149597001411877 1102 6 0.16005733399131827 1102 7 0.14194190680797192 1102 8 0.1256108701258043 1102 9 0.1872618241255708 1102 10-11 0.19367926816648406 1102 12-13 0.2088796618897959 1102 14-15 0.2500845617487002 1102 16-17 0.27113839789551974 1102 18-19 0.28546024797363856 1102 20-21 0.34848086440939596 1102 22-23 0.2576872502300134 1102 24-25 0.25236272870875354 1102 26-27 0.3109655000023963 1102 28-29 0.31233449403482183 1102 30-31 0.3902516974287096 1102 32-33 0.408744249697655 1102 34-35 0.28768309252118485 1102 36-37 0.32648700770581485 1102 38-39 0.32968022121620777 1102 40-41 0.36136306771895477 1102 42-43 0.38540867897413733 1102 44-45 0.35391565421382865 1102 46-47 0.3377599742711439 1102 48-49 0.36301340191666753 1102 50-51 0.38347636707118227 1102 52-53 0.4645175443059273 1102 54-55 0.44431808514785587 1102 56-57 0.4048495708074924 1102 58-59 0.4527495510167654 1102 60-61 0.39402551641473593 1102 62-63 0.4748873664277369 1102 64-65 0.48112979257651034 1102 66-67 0.40891656107549323 1102 68-69 0.42415347954268157 1102 70-71 0.39761509752044333 1102 72-73 0.492243916103277 1102 74-75 0.4667655849934178 1102 76-77 0.5132648305823153 1102 78-79 0.4388988120750099 1102 80-81 0.47658573721631825 1102 82-83 0.4843692036708447 1102 84-85 0.44110406056356055 1102 86-87 0.4141401919697074 1102 88-89 0.5056354923617903 1102 90-91 0.4699269478471777 1102 92-93 0.4764222841417052 1102 94-95 0.501992608412742 1102 96-97 0.503726704034861 1102 98-99 0.4886783396137204 1102 100-101 0.5893670700877962 2305 1 0.1325976793408259 2305 2 0.11818639556183541 2305 3 0.13724166817848982 2305 4 0.14746927029318613 2305 5 0.15815033308721382 2305 6 0.18079645841742575 2305 7 0.16016007974059931 2305 8 0.19047331156001235 2305 9 0.16560011795270668 2305 10-11 0.14162950933961582 2305 12-13 0.17143770817961013 2305 14-15 0.2993288020595912 2305 16-17 0.28894306768636824 2305 18-19 0.3109251628282266 2305 20-21 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-1.091732405564514 1213 82-83 -1.1909415032362602 1213 84-85 -1.1790936398839733 1213 86-87 -1.2191329532105044 1213 88-89 -1.2999192169308955 1213 90-91 -1.4373717125481775 1213 92-93 -1.2989165856927656 1213 94-95 -1.2911186475257992 1213 96-97 -1.2756829788170663 1213 98-99 -1.4099181079574024 1213 100-101 -1.4422971127097384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8329.0 3 673.0 4 416.0 5 381.0 6 325.0 7 695.0 8 1314.0 9 2075.0 10 3650.0 11 5318.0 12 5247.0 13 5237.0 14 5206.0 15 5319.0 16 5718.0 17 6463.0 18 7657.0 19 8530.0 20 9524.0 21 11038.0 22 12484.0 23 14392.0 24 16614.0 25 19247.0 26 22365.0 27 27041.0 28 32926.0 29 40668.0 30 51736.0 31 67068.0 32 89733.0 33 123550.0 34 174979.0 35 265293.0 36 542835.0 37 2461180.0 38 2528080.0 39 917.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.26947401246611 15.085448730381476 20.60549223625515 39.03958502089727 2 25.44686734288804 25.40151214415105 30.666730547228266 18.48488996573264 3 23.0388432551534 27.995517190870288 29.581368720025054 19.384270833951255 4 25.190873247388158 31.670130268329764 21.321591155891884 21.817405328390198 5 26.88790494279514 34.25237380587087 21.49708556046007 17.36263569087392 6 18.642307546641472 37.919987734553466 22.454528184137448 20.983176534667606 7 17.78179800478574 15.823179505400537 43.77510064594712 22.619921843866607 8 21.94626901990008 21.412350673348186 28.094200308728812 28.54717999802292 9 24.096207207097567 23.16763317066047 26.895392422933224 25.84076719930874 10-11 24.019140677010693 31.565068728304148 21.834900140606976 22.580890454078183 12-13 22.566877032603237 25.676586532053552 28.1659797199867 23.590556715356513 14-15 22.91380438269802 27.005198956298322 27.791025951873383 22.289970709130273 16-17 23.633960325280746 27.427030689790055 26.790725963806004 22.14828302112319 18-19 23.522834655492783 27.67024302163477 26.840843506336125 21.96607881653632 20-21 23.742119555374135 27.74430938372191 26.68164601793078 21.831925042973175 22-23 23.700298329668854 27.705033073894285 26.756965749343582 21.837702847093283 24-25 23.302380868692744 28.030156057208046 26.736328732155584 21.931134341943633 26-27 23.60316861550197 28.114293129871566 26.557978563458228 21.72455969116823 28-29 23.633627588196575 27.54298102179385 26.87737737326483 21.94601401674474 30-31 23.333927727499297 27.739681209552842 26.871107842999336 22.055283219948528 32-33 23.669736689380365 27.820032867999977 26.612058567289264 21.898171875330394 34-35 23.666133980558747 27.629088905942755 26.753218460863472 21.951558652635033 36-37 23.3540197950452 27.74886852529393 26.940112662916917 21.956999016743957 38-39 23.731264490923586 27.777274884833293 26.59554577154089 21.895914852702234 40-41 23.884219665626965 27.516065383160814 26.733391114178552 21.866323837033665 42-43 23.64228771765379 27.54401007944563 26.90290620972368 21.910795993176897 44-45 23.96908451665289 27.651771544738086 26.542826423288744 21.83631751532028 46-47 23.93175969465677 27.528749168129572 26.721724545373494 21.81776659184017 48-49 23.683256201525936 27.553175207610504 26.85562887658396 21.907939714279603 50-51 24.13951759937048 27.71660222271155 26.494454786487324 21.64942539143064 52-53 24.192927509493728 27.494220704182275 26.596542591849026 21.716309194474967 54-55 23.967842621067767 27.61610853157217 26.603210870679188 21.812837976680875 56-57 24.299030452839634 27.685943436947795 26.390851341678907 21.624174768533656 58-59 24.48155556490225 27.665409096444048 26.366115927322216 21.48691941133149 60-61 24.258972364000872 27.804658046626102 26.374129839075845 21.56223975029718 62-63 24.641031709119183 27.828202296168154 26.06660822265829 21.464157772054374 64-65 24.80667254678666 27.754929519186096 26.116160462166476 21.322237471860767 66-67 24.641764763647238 27.888186138483423 26.147028596521427 21.323020501347916 68-69 25.158315454488328 27.846638992684497 25.816719992562927 21.17832556026425 70-71 25.355951045730983 27.813567554600304 25.707745393138477 21.12273600653024 72-73 25.30519987053475 27.982970223373194 25.74807447066247 20.963755435429587 74-75 25.747615048472884 28.015716829232122 25.44475608942291 20.79191203287209 76-77 26.016594999579475 27.827636691062274 25.420497865210063 20.735270444148192 78-79 25.99721036410083 27.964361027801772 25.38900459736684 20.649424010730563 80-81 26.52422149869846 27.99292909581212 24.97458727381799 20.508262131671433 82-83 26.766093959194542 27.877670797440718 25.064757494714556 20.291477748650184 84-85 26.72784496865337 27.9903510452692 25.05974689178866 20.22205709428877 86-87 27.218986756036724 28.02697104082833 24.69072168084826 20.063320522286688 88-89 27.545380062587732 27.977480950170158 24.69838656779354 19.778752419448566 90-91 27.603265965343386 28.084309409260882 24.655648263054193 19.656776362341535 92-93 28.057511879472173 28.120334639960554 24.348961116133804 19.473192364433466 94-95 28.389034634574646 28.039734258230087 24.322361148556283 19.248869958638984 96-97 28.340076845463162 28.19246443512926 24.297072713867244 19.170386005540333 98-99 28.816252162312335 28.24156722907194 24.06728725074944 18.874893357866284 100-101 29.046684646286717 28.186621459026394 23.93752918646022 18.829164708226664 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7092.0 1 3891.5 2 560.0 3 353.0 4 254.5 5 232.0 6 225.0 7 233.0 8 227.0 9 212.5 10 212.5 11 216.0 12 230.5 13 255.0 14 283.5 15 307.5 16 331.5 17 374.0 18 447.0 19 521.5 20 594.0 21 709.5 22 900.5 23 1197.0 24 1692.0 25 2493.0 26 3814.0 27 5556.5 28 8221.5 29 12168.0 30 16271.5 31 20945.0 32 27195.0 33 34568.5 34 43913.5 35 56575.0 36 74508.0 37 98097.0 38 126792.0 39 161562.0 40 203636.5 41 251300.0 42 302661.5 43 352819.0 44 397742.0 45 435244.0 46 460125.5 47 469720.0 48 462502.5 49 440032.5 50 403664.0 51 356443.5 52 302687.5 53 250581.0 54 200394.0 55 154043.0 56 117307.5 57 88038.0 58 65088.0 59 47422.5 60 33460.0 61 22471.0 62 15827.5 63 11462.5 64 7883.5 65 5604.0 66 4076.5 67 3049.5 68 2458.0 69 1813.5 70 1269.0 71 810.5 72 480.5 73 312.5 74 284.0 75 253.0 76 182.0 77 101.0 78 57.5 79 30.0 80 26.0 81 31.0 82 29.0 83 23.0 84 17.0 85 11.5 86 9.5 87 8.5 88 8.0 89 7.5 90 6.5 91 5.0 92 4.0 93 3.5 94 1.5 95 0.5 96 1.5 97 1.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15792296220829702 2 0.1435704714132556 3 0.14844576193728554 4 0.13940900847374094 5 0.16582062910080658 6 0.14665359906552375 7 0.14718517279867344 8 0.13476153526391801 9 0.16255524759717283 10-11 0.13369079388714505 12-13 0.12366683206203677 14-15 0.18283858247206997 16-17 0.1914804525909891 18-19 0.17490294602719259 20-21 0.21027538101306714 22-23 0.19442688985473305 24-25 0.18544329376450341 26-27 0.17002006159268906 28-29 0.1878581572950977 30-31 0.13517920033996417 32-33 0.16184142001265753 34-35 0.16605604032548715 36-37 0.16955683305380148 38-39 0.16923788881391166 40-41 0.20379018146864103 42-43 0.18883017783571426 44-45 0.17314115879732506 46-47 0.1774696877672582 48-49 0.21883371811677704 50-51 0.19561913379908305 52-53 0.1601024145142107 54-55 0.14610683751142695 56-57 0.14278070472400464 58-59 0.15647252530784575 60-61 0.15199971203891485 62-63 0.14077591235898299 64-65 0.16191735911739322 66-67 0.16590416211601583 68-69 0.1783657692031391 70-71 0.15941136866111613 72-73 0.16752925895735912 74-75 0.20013751053085538 76-77 0.22896399468851528 78-79 0.21007034543028083 80-81 0.18560276588444832 82-83 0.15603207850037887 84-85 0.16506883196392347 86-87 0.15505246404928874 88-89 0.15812040388060974 90-91 0.170407351026841 92-93 0.15449810858471835 94-95 0.1487723000876489 96-97 0.1556751647081212 98-99 0.1467751016331008 100-101 0.1535944332383639 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6584223.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.18814390999413 #Duplication Level Percentage of deduplicated Percentage of total 1 71.85908864616954 38.2205154815349 2 15.312695701782605 16.289077252727235 3 5.072294984390538 8.093578667511162 4 2.432449687082161 5.175099360413847 5 1.4884469014534687 3.958386399844597 6 0.8040033352190628 2.5658067058648055 7 0.6226190148319349 2.318116483338581 8 0.4103090211346647 1.7458860210943494 9 0.26588642582842603 1.272780493260867 >10 1.6318984552973357 15.305409185075902 >50 0.0738531643777481 2.6407593739824553 >100 0.026073150309980985 2.2866856612579363 >500 3.4539519271478014E-4 0.10332942052889381 >1k 3.611692980558925E-5 0.02456949356451065 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.2781731420700666E-4 0.0 0.0 2 2.4300513515414042E-4 0.0 0.0 3 2.4300513515414042E-4 0.0 0.0 4 3.0375641894267555E-4 0.0 0.0 5 3.341320608369431E-4 0.0 0.0 6 3.4931988178407686E-4 0.0 0.0 7 3.4931988178407686E-4 0.0 0.0 8 4.708224493611471E-4 0.0 0.0 9 5.163859122025484E-4 0.0 0.0 10-11 5.46761554096816E-4 0.0 0.0 12-13 5.771371959910835E-4 1.5187820947133777E-5 0.0 14-15 7.290154054624213E-4 1.5187820947133777E-5 0.0 16-17 9.188631673015936E-4 3.7969552367834444E-5 0.0 18-19 0.001093523108193632 6.0751283788535106E-5 0.0 20-21 0.0019136654393388558 6.0751283788535106E-5 0.0 22-23 0.00561189983996593 6.0751283788535106E-5 0.0 24-25 0.010532753826837275 7.593910473566889E-5 0.0 26-27 0.020412431352947798 7.593910473566889E-5 0.0 28-29 0.045593838483295596 8.353301520923578E-5 0.0 30-31 0.0876109451335412 9.112692568280266E-5 0.0 32-33 0.14742817793382756 9.112692568280266E-5 0.0 34-35 0.2104956044168006 1.1390865710350333E-4 0.0 36-37 0.29242630451611373 1.2150256757707021E-4 0.0 38-39 0.39786775144159 1.2150256757707021E-4 0.0 40-41 0.5330545456920277 1.2150256757707021E-4 0.0 42-43 0.7042668512290668 1.2150256757707021E-4 0.0 44-45 0.9014579244961782 1.36690388524204E-4 0.0 46-47 1.1260402328414454 1.36690388524204E-4 0.0 48-49 1.3863974534276862 1.4428429899777088E-4 0.0 50-51 1.6935863199044139 1.6706603041847156E-4 0.0 52-53 2.0593773935056574 1.8225385136560533E-4 0.0 54-55 2.4777866727782456 2.1262949325987287E-4 0.0 56-57 2.9454880249347566 2.2022340373343977E-4 0.0 58-59 3.4771149154577543 2.3541122468057356E-4 0.0 60-61 4.094590963884425 2.505990456277073E-4 0.0 62-63 4.8007714805528305 2.581929561012742E-4 0.0 64-65 5.59065207846089 2.581929561012742E-4 0.0 66-67 6.463739153427823 2.581929561012742E-4 0.0 68-69 7.420382025335412 2.581929561012742E-4 0.0 70-71 8.462577892638205 2.581929561012742E-4 0.0 72-73 9.58793467353703 2.657868665748411E-4 0.0 74-75 10.79361224551477 2.73380777048408E-4 7.593910473566888E-6 76-77 12.066959153722467 2.73380777048408E-4 1.5187820947133777E-5 78-79 13.408241185026693 2.73380777048408E-4 1.5187820947133777E-5 80-81 14.825530666260848 2.73380777048408E-4 1.5187820947133777E-5 82-83 16.31517492648715 2.8856859799554176E-4 1.5187820947133777E-5 84-85 17.869185171887402 3.0375641894267555E-4 1.5187820947133777E-5 86-87 19.48333918823831 3.0375641894267555E-4 1.5187820947133777E-5 88-89 21.157515472972285 3.0375641894267555E-4 1.5187820947133777E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGCC 500 0.0 19.480377 42-43 GGCGCCC 185 2.743036E-9 16.693926 44-45 GGGGCGC 515 0.0 16.606527 42-43 GGGGGCG 670 0.0 14.53483 40-41 CGAAAAT 4150 0.0 12.472704 1 TCGGGGG 1025 0.0 12.049861 38-39 CAAAAAT 7470 0.0 10.61642 1 GGGGCCC 280 7.4807904E-6 10.18146 44-45 GGGGGGC 830 0.0 10.015921 40-41 AAAAATC 5500 0.0 9.756515 2 CGCGAAT 985 2.0008883E-10 9.642198 1 CTTGAAT 4350 0.0 9.606732 1 CGGCAAC 1640 0.0 9.555476 1 CGGGTAG 655 0.0 9.429834 14-15 CACGAAT 1575 0.0 9.346809 1 CGAAAAC 4115 0.0 9.232141 1 CGGGGGG 1085 0.0 9.194374 38-39 GCGCCCG 325 4.3600226E-5 8.771719 44-45 CACATAG 1695 0.0 8.687533 9 CGAACAT 2100 0.0 8.593035 1 >>END_MODULE