FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n02 tsrexp_lib#38.3420000000d103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n02 tsrexp_lib#38.3420000000d103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6584223
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC5000.019.48037742-43
GGCGCCC1852.743036E-916.69392644-45
GGGGCGC5150.016.60652742-43
GGGGGCG6700.014.5348340-41
CGAAAAT41500.012.4727041
TCGGGGG10250.012.04986138-39
CAAAAAT74700.010.616421
GGGGCCC2807.4807904E-610.1814644-45
GGGGGGC8300.010.01592140-41
AAAAATC55000.09.7565152
CGCGAAT9852.0008883E-109.6421981
CTTGAAT43500.09.6067321
CGGCAAC16400.09.5554761
CGGGTAG6550.09.42983414-15
CACGAAT15750.09.3468091
CGAAAAC41150.09.2321411
CGGGGGG10850.09.19437438-39
GCGCCCG3254.3600226E-58.77171944-45
CACATAG16950.08.6875339
CGAACAT21000.08.5930351
Produced by FastQC (version 0.11.2)