##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l02n02 tsrexp_lib#35.3420000000d0f5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8299067 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.600817537682246 33.0 33.0 33.0 33.0 33.0 2 32.55078504607807 33.0 33.0 33.0 33.0 33.0 3 32.53989972607764 33.0 33.0 33.0 33.0 33.0 4 36.427551072909765 37.0 37.0 37.0 37.0 37.0 5 36.41506521154727 37.0 37.0 37.0 37.0 37.0 6 36.43367296588882 37.0 37.0 37.0 37.0 37.0 7 36.456061747663924 37.0 37.0 37.0 37.0 37.0 8 36.46755882317856 37.0 37.0 37.0 37.0 37.0 9 36.4324165595964 37.0 37.0 37.0 37.0 37.0 10-11 36.41540934661691 37.0 37.0 37.0 37.0 37.0 12-13 36.41767996330189 37.0 37.0 37.0 37.0 37.0 14-15 38.779968459105106 40.0 40.0 40.0 37.0 40.0 16-17 38.756984188704585 40.0 40.0 40.0 37.0 40.0 18-19 38.75996675288921 40.0 40.0 40.0 37.0 40.0 20-21 38.706396273219625 40.0 40.0 40.0 37.0 40.0 22-23 38.72829855452426 40.0 40.0 40.0 37.0 40.0 24-25 38.70021539770676 40.0 40.0 40.0 37.0 40.0 26-27 38.67961181660541 40.0 40.0 40.0 37.0 40.0 28-29 38.635714412234535 40.0 40.0 40.0 37.0 40.0 30-31 38.62032424849685 40.0 40.0 40.0 37.0 40.0 32-33 38.541502255615 40.0 40.0 40.0 37.0 40.0 34-35 38.52867635602894 40.0 40.0 40.0 37.0 40.0 36-37 38.49791639228843 40.0 40.0 40.0 37.0 40.0 38-39 38.47687824426529 40.0 40.0 40.0 37.0 40.0 40-41 38.43065334934637 40.0 38.5 40.0 37.0 40.0 42-43 38.37711510221571 40.0 37.0 40.0 37.0 40.0 44-45 38.33068542524117 40.0 37.0 40.0 37.0 40.0 46-47 38.247334128041146 40.0 37.0 40.0 37.0 40.0 48-49 38.14731975293127 40.0 37.0 40.0 37.0 40.0 50-51 36.77554711873033 38.5 37.0 38.5 35.0 40.0 52-53 37.09719502204284 38.5 37.0 40.0 33.0 40.0 54-55 37.768446742266335 40.0 37.0 40.0 35.0 40.0 56-57 37.72015914560034 40.0 37.0 40.0 35.0 40.0 58-59 37.519775897700306 40.0 37.0 40.0 33.0 40.0 60-61 37.324432915169865 37.0 37.0 40.0 33.0 40.0 62-63 37.094712453821614 37.0 37.0 40.0 33.0 40.0 64-65 36.813564103049174 37.0 37.0 40.0 33.0 40.0 66-67 36.56015429204271 37.0 37.0 40.0 33.0 40.0 68-69 36.281099851344734 37.0 37.0 37.0 33.0 40.0 70-71 36.02159513834506 37.0 37.0 37.0 33.0 40.0 72-73 35.72744749500155 37.0 37.0 37.0 33.0 38.5 74-75 35.57241139275054 37.0 37.0 37.0 33.0 37.0 76-77 35.36015885882112 37.0 37.0 37.0 33.0 37.0 78-79 35.259880417883124 37.0 37.0 37.0 33.0 37.0 80-81 35.16007727133665 37.0 37.0 37.0 33.0 37.0 82-83 35.08337232366 37.0 37.0 37.0 33.0 37.0 84-85 35.03232965826159 37.0 35.0 37.0 33.0 37.0 86-87 34.96938113645787 37.0 33.0 37.0 33.0 37.0 88-89 34.887052544581216 37.0 33.0 37.0 33.0 37.0 90-91 34.82516106931057 37.0 33.0 37.0 33.0 37.0 92-93 34.797318843190446 37.0 33.0 37.0 33.0 37.0 94-95 34.73437712938093 37.0 33.0 37.0 33.0 37.0 96-97 34.657553493663805 37.0 33.0 37.0 33.0 37.0 98-99 34.590261351065124 37.0 33.0 37.0 33.0 37.0 100-101 32.71917487833271 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12276950127520792 2306 2 0.15560837329419996 2306 3 0.19602094911896017 2306 4 0.23406057733487984 2306 5 0.2666610042105759 2306 6 0.28946545574350324 2306 7 0.26108247316875577 2306 8 0.25894585944746495 2306 9 0.2382390955581144 2306 10-11 0.2474632428396859 2306 12-13 0.26431040212906254 2306 14-15 0.4836823037680489 2306 16-17 0.4784657170228428 2306 18-19 0.49018953053555236 2306 20-21 0.5325056999366566 2306 22-23 0.5337519124604668 2306 24-25 0.5473992089039044 2306 26-27 0.5377595240558009 2306 28-29 0.5805027243546661 2306 30-31 0.5759598566964641 2306 32-33 0.6390499198920025 2306 34-35 0.62017735902959 2306 36-37 0.5603457901193281 2306 38-39 0.5532652446692694 2306 40-41 0.6186762825839978 2306 42-43 0.5965740915255182 2306 44-45 0.6159543759740416 2306 46-47 0.6256720492697454 2306 48-49 0.6239980234467808 2306 50-51 0.5390405546645454 2306 52-53 0.6386826566927439 2306 54-55 0.6550751495757012 2306 56-57 0.5925724678501112 2306 58-59 0.6372478526485921 2306 60-61 0.5828961679331073 2306 62-63 0.6032579456490481 2306 64-65 0.5917109344622773 2306 66-67 0.6015134310954764 2306 68-69 0.5995828439448729 2306 70-71 0.6120745356193495 2306 72-73 0.6881919394539509 2306 74-75 0.6758919964826902 2306 76-77 0.718727474507169 2306 78-79 0.7096634489160536 2306 80-81 0.6974640905268217 2306 82-83 0.697869349657033 2306 84-85 0.7016063606031153 2306 86-87 0.691043354858536 2306 88-89 0.719250093138939 2306 90-91 0.751755253993224 2306 92-93 0.7948793018987104 2306 94-95 0.7795195769598493 2306 96-97 0.7911824233641269 2306 98-99 0.7554824236423201 2306 100-101 0.7956551375500283 2307 1 0.14286426158736276 2307 2 0.17011947037517672 2307 3 0.1928975843884544 2307 4 0.250578221181712 2307 5 0.1797866725381141 2307 6 0.2616062936840251 2307 7 0.23626377478639426 2307 8 0.27849458194649657 2307 9 0.2971117834292514 2307 10-11 0.2904968526046616 2307 12-13 0.26976075534727784 2307 14-15 0.45352373950622393 2307 16-17 0.4502977023253223 2307 18-19 0.4534360666081767 2307 20-21 0.5304515170594826 2307 22-23 0.5278802996012715 2307 24-25 0.5140866363832544 2307 26-27 0.56042179966839 2307 28-29 0.5968952345359995 2307 30-31 0.5207555759875291 2307 32-33 0.6319156656886449 2307 34-35 0.6347978058705408 2307 36-37 0.6196480561762527 2307 38-39 0.5663000488919607 2307 40-41 0.5377029275229077 2307 42-43 0.5243591687671199 2307 44-45 0.5639545835821735 2307 46-47 0.6322938953325874 2307 48-49 0.58270611788857 2307 50-51 0.5356119633283498 2307 52-53 0.5890262054834992 2307 54-55 0.6474811002502818 2307 56-57 0.6062503595753626 2307 58-59 0.5984102022038016 2307 60-61 0.5635210683949623 2307 62-63 0.5908512271087858 2307 64-65 0.6211280817559768 2307 66-67 0.5300103482569867 2307 68-69 0.5653067421998301 2307 70-71 0.5708240805878404 2307 72-73 0.6745271298846376 2307 74-75 0.6042066166451292 2307 76-77 0.592410727072405 2307 78-79 0.6378809482903804 2307 80-81 0.6495442955185169 2307 82-83 0.7472342252059079 2307 84-85 0.7362540951149796 2307 86-87 0.7802618808977115 2307 88-89 0.8029805125642326 2307 90-91 0.8185525289232629 2307 92-93 0.7655412163300284 2307 94-95 0.7491695439700692 2307 96-97 0.7616847212732623 2307 98-99 0.8103420890804074 2307 100-101 0.8894303775791528 2201 1 0.1844668709068742 2201 2 0.22217180933423464 2201 3 0.22784407397200823 2201 4 0.2700198490510033 2201 5 0.2789268983620232 2201 6 0.3118411572943245 2201 7 0.23797020714681594 2201 8 0.22413334858556766 2201 9 0.2296379418967618 2201 10-11 0.2792732056281082 2201 12-13 0.3065445603703054 2201 14-15 0.46654040776624583 2201 16-17 0.508715911259138 2201 18-19 0.5014370898031828 2201 20-21 0.585027577077085 2201 22-23 0.4989225337099228 2201 24-25 0.48330570457447664 2201 26-27 0.5350067357426838 2201 28-29 0.5758998353453748 2201 30-31 0.5864160947483725 2201 32-33 0.6582119372403739 2201 34-35 0.6397424486338608 2201 36-37 0.6764458064760674 2201 38-39 0.6162949858238491 2201 40-41 0.5886392690692404 2201 42-43 0.5757658896424473 2201 44-45 0.6038885643730225 2201 46-47 0.6321958329927426 2201 48-49 0.5779708265320664 2201 50-51 0.5619454542750475 2201 52-53 0.6567859904009197 2201 54-55 0.6488765199638706 2201 56-57 0.6720220443638709 2201 58-59 0.686588024292142 2201 60-61 0.6387745632346267 2201 62-63 0.6412803390491035 2201 64-65 0.6496985506206912 2201 66-67 0.6762706357285211 2201 68-69 0.6806906808681745 2201 70-71 0.6839190264646859 2201 72-73 0.7608291252256407 2201 74-75 0.6441945501787956 2201 76-77 0.7231693518520572 2201 78-79 0.7065130512427942 2201 80-81 0.7401280285143343 2201 82-83 0.7389230112667349 2201 84-85 0.7538813851382287 2201 86-87 0.7978951998477513 2201 88-89 0.8274804407707848 2201 90-91 0.7630561962948192 2201 92-93 0.7703736779912802 2201 94-95 0.8341160766173843 2201 96-97 0.8000044569158931 2201 98-99 0.6844033451913063 2201 100-101 0.7765763734551143 1101 1 0.11746050133994856 1101 2 0.17496011852879434 1101 3 0.10930402718745569 1101 4 0.20609705431017744 1101 5 0.21981840365380378 1101 6 0.2687165748393454 1101 7 0.22366750364330557 1101 8 0.2310468462865316 1101 9 0.25004457404095604 1101 10-11 0.24264020565055233 1101 12-13 0.25267923322883945 1101 14-15 0.40395455735886543 1101 16-17 0.41148869779474495 1101 18-19 0.4337336885777816 1101 20-21 0.48225124436290656 1101 22-23 0.47362525115806164 1101 24-25 0.4969838254979706 1101 26-27 0.4799626168297664 1101 28-29 0.5020018503500623 1101 30-31 0.46653742807350795 1101 32-33 0.5676563160796704 1101 34-35 0.5018189847706438 1101 36-37 0.506642022188359 1101 38-39 0.5185696318024426 1101 40-41 0.5373476973153686 1101 42-43 0.5264600778559014 1101 44-45 0.5163893840204352 1101 46-47 0.4780905911156097 1101 48-49 0.49201931054572867 1101 50-51 0.5579850380307079 1101 52-53 0.5702832412122234 1101 54-55 0.591343623905864 1101 56-57 0.5892778693373018 1101 58-59 0.6223241661989007 1101 60-61 0.5840380965699339 1101 62-63 0.5896097163176393 1101 64-65 0.5708521960347426 1101 66-67 0.5123508569900537 1101 68-69 0.5590141166620555 1101 70-71 0.6144824992348603 1101 72-73 0.6380989608555012 1101 74-75 0.5928161245590431 1101 76-77 0.6432624948081198 1101 78-79 0.6057274801582437 1101 80-81 0.6646435412449847 1101 82-83 0.6579687561200345 1101 84-85 0.7218942792235765 1101 86-87 0.6942724608304403 1101 88-89 0.7149492910431761 1101 90-91 0.7300517022512238 1101 92-93 0.6914118100144648 1101 94-95 0.7002050462864489 1101 96-97 0.6926960929811443 1101 98-99 0.6200322014416813 1101 100-101 0.6204078208366539 2304 1 0.09120415305663698 2304 2 0.13793442754391094 2304 3 0.13693744861745216 2304 4 0.14174449866408168 2304 5 0.15928398183911696 2304 6 0.20029877090637882 2304 7 0.11492361616295454 2304 8 0.2094528150970234 2304 9 0.18157317388003946 2304 10-11 0.19837112954327552 2304 12-13 0.237426625800083 2304 14-15 0.3849195753654513 2304 16-17 0.3661938470651407 2304 18-19 0.4043635744062968 2304 20-21 0.42910582640300987 2304 22-23 0.44818761086001047 2304 24-25 0.41788185248682197 2304 26-27 0.4584269611496907 2304 28-29 0.41806977332768014 2304 30-31 0.3536175580614227 2304 32-33 0.4333015606839439 2304 34-35 0.38574525837982065 2304 36-37 0.4458713886540622 2304 38-39 0.45848770553011065 2304 40-41 0.46485762552586607 2304 42-43 0.44609929757846345 2304 44-45 0.4626862836536034 2304 46-47 0.46475669450980917 2304 48-49 0.42809686889616927 2304 50-51 0.4545570770578138 2304 52-53 0.5107750207414128 2304 54-55 0.47480417360430494 2304 56-57 0.5095365394126148 2304 58-59 0.5440620452263261 2304 60-61 0.5706123054761676 2304 62-63 0.5492966051056811 2304 64-65 0.5833473927501842 2304 66-67 0.535972242311729 2304 68-69 0.5011435041864729 2304 70-71 0.5242112174038382 2304 72-73 0.5826872460258414 2304 74-75 0.5553297663139176 2304 76-77 0.5833561431217049 2304 78-79 0.6135133137278714 2304 80-81 0.6182086611953537 2304 82-83 0.6367404466436355 2304 84-85 0.6306376119659021 2304 86-87 0.6454398323344392 2304 88-89 0.6299456308389892 2304 90-91 0.6641063017015654 2304 92-93 0.6002632526640497 2304 94-95 0.5732300562237285 2304 96-97 0.6437471828600039 2304 98-99 0.5302258114143399 2304 100-101 0.6852198915947696 2202 1 0.10608550456388599 2202 2 0.12706447991831027 2202 3 0.14558296246712388 2202 4 0.14718466045460588 2202 5 0.21590069887937346 2202 6 0.18108889805460393 2202 7 0.20249493226179283 2202 8 0.191416317766695 2202 9 0.20354948471837275 2202 10-11 0.24690213226593727 2202 12-13 0.22631795681946443 2202 14-15 0.36950486627711854 2202 16-17 0.3540080869669069 2202 18-19 0.38818448467138467 2202 20-21 0.38861373462898285 2202 22-23 0.37078863603417034 2202 24-25 0.42393573902593573 2202 26-27 0.4280164117409839 2202 28-29 0.4679622204130922 2202 30-31 0.4443279911776088 2202 32-33 0.48998125865810493 2202 34-35 0.5507368776573784 2202 36-37 0.45850296153362535 2202 38-39 0.4819651084470564 2202 40-41 0.539251831503087 2202 42-43 0.47949654159081234 2202 44-45 0.4937621958544227 2202 46-47 0.4620135559357621 2202 48-49 0.47452119935882564 2202 50-51 0.4127729928809174 2202 52-53 0.5533728922201391 2202 54-55 0.542853538606991 2202 56-57 0.5138564327225126 2202 58-59 0.49800749298970715 2202 60-61 0.5035663771833185 2202 62-63 0.5038409202907559 2202 64-65 0.4806564314718358 2202 66-67 0.49356861318184997 2202 68-69 0.4750723169503104 2202 70-71 0.5310534534185152 2202 72-73 0.57703960231607 2202 74-75 0.5458318249555418 2202 76-77 0.6126192383026918 2202 78-79 0.6033030202594105 2202 80-81 0.6419026575336417 2202 82-83 0.6552094799950865 2202 84-85 0.57004275732411 2202 86-87 0.6145425427645819 2202 88-89 0.6182449001709074 2202 90-91 0.6634761576542942 2202 92-93 0.6398986772916615 2202 94-95 0.6066249758732951 2202 96-97 0.6717125212184314 2202 98-99 0.6308246416462282 2202 100-101 0.6608482970186813 1102 1 0.045232329452147724 1102 2 0.05374194470855542 1102 3 0.05783519450275065 1102 4 0.08724763404305946 1102 5 0.0726913487590437 1102 6 0.09650633147914078 1102 7 0.08503005940539765 1102 8 0.07741715371030011 1102 9 0.06660691083024517 1102 10-11 0.10755217489204227 1102 12-13 0.07831686863296028 1102 14-15 0.11584055438675023 1102 16-17 0.16189759190258712 1102 18-19 0.20273887733323903 1102 20-21 0.18817875708273135 1102 22-23 0.1388748564821256 1102 24-25 0.15905193682804963 1102 26-27 0.22016336829574357 1102 28-29 0.20242000087588252 1102 30-31 0.23769423491022934 1102 32-33 0.2407870723080876 1102 34-35 0.1253597680158478 1102 36-37 0.17113830798369634 1102 38-39 0.20940601822800886 1102 40-41 0.25547470796499994 1102 42-43 0.19984781613887748 1102 44-45 0.2590753287072687 1102 46-47 0.20162896246582562 1102 48-49 0.2212143871766301 1102 50-51 0.20997021101179314 1102 52-53 0.2730476007276721 1102 54-55 0.26872725216396276 1102 56-57 0.258597425718726 1102 58-59 0.2784761068832964 1102 60-61 0.2942536858969973 1102 62-63 0.3124688779137301 1102 64-65 0.3124394300401363 1102 66-67 0.31865708916114244 1102 68-69 0.3300424243561082 1102 70-71 0.3078901345153042 1102 72-73 0.4301213536286781 1102 74-75 0.37815672422105706 1102 76-77 0.47332709366600056 1102 78-79 0.36329176551847553 1102 80-81 0.385061332745785 1102 82-83 0.38147437374157533 1102 84-85 0.35918886927819926 1102 86-87 0.3376561118765551 1102 88-89 0.3932148678189691 1102 90-91 0.39420915413189306 1102 92-93 0.36781766318693343 1102 94-95 0.4587045171465505 1102 96-97 0.3697373003205868 1102 98-99 0.37718441957515125 1102 100-101 0.39012263584154994 2305 1 0.0996635542487212 2305 2 0.11307609776530114 2305 3 0.08603531693505317 2305 4 0.12954283620544516 2305 5 0.13572272335100877 2305 6 0.1857904182622434 2305 7 0.13985496192437807 2305 8 0.15708928773332076 2305 9 0.15649226064223853 2305 10-11 0.1410079288300281 2305 12-13 0.14215283507926557 2305 14-15 0.30427065542555454 2305 16-17 0.28913600687791785 2305 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-0.968207025039419 1213 74-75 -1.1069617060501358 1213 76-77 -1.0016252982082747 1213 78-79 -1.134041454940835 1213 80-81 -1.1014439164406085 1213 82-83 -1.1170995092090905 1213 84-85 -1.0525172632671556 1213 86-87 -1.1204394636659032 1213 88-89 -1.21738730752557 1213 90-91 -1.3238490885638967 1213 92-93 -1.1953670299717132 1213 94-95 -1.162982995534911 1213 96-97 -1.2231223521779597 1213 98-99 -1.3298715965781014 1213 100-101 -1.4333044510367756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11258.0 3 844.0 4 585.0 5 480.0 6 419.0 7 794.0 8 1397.0 9 2655.0 10 5934.0 11 9641.0 12 8721.0 13 7760.0 14 7271.0 15 7018.0 16 7318.0 17 8327.0 18 9322.0 19 10712.0 20 11658.0 21 13130.0 22 15091.0 23 17265.0 24 20364.0 25 23348.0 26 27226.0 27 33022.0 28 39831.0 29 50060.0 30 63650.0 31 82683.0 32 111792.0 33 154916.0 34 218876.0 35 331168.0 36 676495.0 37 3068174.0 38 3238534.0 39 1328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.27741271425254 14.527943170785521 19.140344194476107 38.05429992048583 2 25.505424973559375 25.070150312098292 30.328768796252987 19.095655918089342 3 23.54256214784177 27.74020860125358 29.32031047852969 19.39691877237496 4 25.731194779969417 31.660180082945892 21.425021914242905 21.183603222841786 5 27.169077064117197 34.15693826916101 21.616059686683535 17.05792498003825 6 19.2427655362135 37.751129739126654 22.72691781098826 20.27918691367158 7 18.189610050751707 16.051782114637085 43.51926441948596 22.239343415125244 8 22.363309767480473 21.64204697113549 28.013503344703572 27.981139916680466 9 24.355480601139035 23.356776839922176 27.03544432721417 25.25229823172462 10-11 24.456140683244108 31.495624358308905 21.863752399865117 22.18448255858187 12-13 23.038470518041972 25.634520124492717 28.045254393834 23.281754963631307 14-15 23.187601902440466 26.982993573015722 27.810551554410246 22.01885297013357 16-17 24.02845908806871 27.34204338613983 26.836769849796777 21.79272767599468 18-19 23.88214006291661 27.535004743674385 26.930368847694552 21.65248634571445 20-21 23.9695586813061 27.740397060206075 26.760586512062957 21.52945774642487 22-23 24.112591662789843 27.58057324190911 26.793909001351462 21.512926093949584 24-25 23.59934587969402 28.00161900208623 26.805941894606704 21.593093223613042 26-27 23.866041293462672 28.11495970603999 26.6075274138955 21.411471586601834 28-29 23.959868037801797 27.592263369020277 26.876136030932035 21.57173256224589 30-31 23.70457177168944 27.65607284058893 26.937974627333222 21.701380760388407 32-33 23.863788682495606 27.819645933666383 26.74284912128574 21.573716262552274 34-35 23.94445201214585 27.656937647176623 26.79067289284966 21.607937447827872 36-37 23.557706526107047 27.7035529842946 27.05288211236328 21.68585837723508 38-39 23.880918971278177 27.707237892569886 26.781685444587776 21.630157691564158 40-41 24.22213911697549 27.455422937345958 26.79028243399945 21.5321555116791 42-43 23.969369724287315 27.454155490864302 26.982080905977533 21.594393878870854 44-45 24.23638749764678 27.586062036625535 26.689029015382857 21.488521450344823 46-47 24.21082163956089 27.480977405155027 26.755034659545363 21.55316629573872 48-49 23.8959995468674 27.477878687272927 26.92524290358964 21.70087886227003 50-51 24.38272585047821 27.6411591524142 26.60094757110496 21.375167426002626 52-53 24.43237206890552 27.486655453145488 26.74059680708553 21.34037567086346 54-55 24.21317423717817 27.593468709563513 26.677330656414043 21.516026396844275 56-57 24.46349025713959 27.631329570766262 26.643421898574815 21.261758273519337 58-59 24.693081086241694 27.718291251416154 26.453265907156602 21.13536175518555 60-61 24.51773815046845 27.880802023355095 26.39821358484627 21.203246241330184 62-63 24.874629070271194 27.955852875904625 26.08262606783715 21.086891985987034 64-65 25.087802647097273 27.88669043798786 26.08942936638095 20.93607754853392 66-67 24.946915831219442 27.993961000593277 26.109933206469684 20.949189961717597 68-69 25.426152662801112 27.975478253195856 25.814376028889708 20.783993055113328 70-71 25.64775333602692 27.925222545123834 25.702628840206547 20.7243952786427 72-73 25.662979687433424 28.031268245936115 25.712476457197337 20.593275609433125 74-75 26.08297101576149 28.07402616800853 25.429120974724928 20.413881841505045 76-77 26.392098190684525 27.873107679508426 25.39922063638326 20.335573493423794 78-79 26.420783773308592 27.980193520561446 25.35260524448921 20.24641746164075 80-81 26.94701301426371 28.03073112247821 24.94044488304693 20.081810980211152 82-83 27.229517248870742 27.938065193580215 24.95589491057985 19.87652264696919 84-85 27.272820546308964 28.02842215023928 24.930863563744666 19.767893739707088 86-87 27.677997381682374 28.09512504596901 24.61625908382056 19.610618488528054 88-89 28.105404386653415 28.040167397751432 24.539276589987153 19.315151625608 90-91 28.14475528881938 28.135056071975313 24.531365468663502 19.18882317054181 92-93 28.583688407502983 28.201260144936764 24.220378178657338 18.994673268902915 94-95 28.970236640183565 28.142199051614497 24.135055174245306 18.752509133956632 96-97 28.96088978482327 28.25107488187856 24.113315234675653 18.674720098622515 98-99 29.406702387777468 28.299485532250163 23.91156602356237 18.382246056410004 100-101 29.68211339292913 28.26847941898205 23.741689795157768 18.307717392931057 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9667.0 1 5303.5 2 728.5 3 448.5 4 337.0 5 282.0 6 273.5 7 290.5 8 285.5 9 281.5 10 299.0 11 305.0 12 312.5 13 329.0 14 347.5 15 398.0 16 454.0 17 488.0 18 535.5 19 631.0 20 773.5 21 946.0 22 1232.5 23 1670.5 24 2492.5 25 3677.0 26 5422.5 27 8034.5 28 11747.0 29 16955.0 30 22631.5 31 29499.0 32 38338.5 33 47951.0 34 59129.5 35 74348.0 36 96315.5 37 123958.5 38 157384.0 39 198646.0 40 249440.5 41 307626.5 42 369556.0 43 432716.5 44 492621.5 45 541814.5 46 576670.5 47 592282.5 48 585451.5 49 561126.5 50 517983.5 51 457168.5 52 386518.5 53 319004.5 54 253912.5 55 193706.5 56 146214.5 57 109249.5 58 80785.5 59 58733.0 60 41633.5 61 28709.0 62 20521.5 63 14737.0 64 10455.5 65 7955.0 66 6131.0 67 4440.5 68 3480.5 69 2815.0 70 2251.5 71 1606.0 72 904.5 73 588.0 74 548.5 75 465.5 76 379.0 77 194.0 78 74.0 79 45.5 80 32.5 81 27.0 82 23.5 83 22.0 84 25.5 85 23.5 86 16.5 87 11.5 88 10.5 89 10.5 90 9.0 91 5.5 92 3.0 93 2.5 94 2.0 95 2.5 96 1.5 97 0.0 98 0.0 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1656933243218786 2 0.15152305674842728 3 0.15687305573024052 4 0.14723341792517158 5 0.17438104789369696 6 0.15498127681099574 7 0.15712609622262358 8 0.1438113465043721 9 0.17264591308878455 10-11 0.14317874527341448 12-13 0.13293060533190057 14-15 0.193057846141018 16-17 0.20148650444682517 18-19 0.18561122593660226 20-21 0.2202958477139659 22-23 0.20416752871135996 24-25 0.19504602143831348 26-27 0.18010458284045666 28-29 0.19751617862586243 30-31 0.1450644994190311 32-33 0.17186871729075087 34-35 0.17580891924357278 36-37 0.17891167766207935 38-39 0.1786405628488118 40-41 0.21368667104386552 42-43 0.19854641491627917 44-45 0.18318324216445053 46-47 0.18752107917673155 48-49 0.22809792956244357 50-51 0.2054447807205316 52-53 0.1702420284111455 54-55 0.15574039828814493 56-57 0.15199901386505255 58-59 0.1669766011046784 60-61 0.16174107282180034 62-63 0.15010121017217962 64-65 0.17223622848206913 66-67 0.17570649809189395 68-69 0.18752107917673155 70-71 0.16808515945226132 72-73 0.17698375010106557 74-75 0.20999950958342667 76-77 0.23795445921812658 78-79 0.21980181627645612 80-81 0.19512434349547966 82-83 0.16558487839657157 84-85 0.17523054097526866 86-87 0.1638015454026338 88-89 0.1673682113905093 90-91 0.1794539072886145 92-93 0.16377142153449298 94-95 0.15773459835906856 96-97 0.16553668020754622 98-99 0.15580667079805477 100-101 0.16231945109010446 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8299067.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.84492973252087 #Duplication Level Percentage of deduplicated Percentage of total 1 71.3554729536968 35.5670853540781 2 14.390166004347735 14.345536266520467 3 5.6342144483193195 8.425110698232908 4 2.6064674665454035 5.196767508802293 5 1.3774826765335215 3.433026360978907 6 0.9653722670094308 2.887134768888567 7 0.7101075036024366 2.477668103371948 8 0.5029396807550909 2.005519443754719 9 0.3528191402795754 1.5827620729971472 >10 1.9564209210341366 17.221420587785737 >50 0.1129551796503811 3.7729164110045676 >100 0.03491290394521796 2.8259931922979287 >500 5.709642794521654E-4 0.18477476829503325 >1k 9.789000159094949E-5 0.07428446299167582 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.4459456707603398E-4 0.0 0.0 2 1.5664411433237012E-4 0.0 0.0 3 1.8074320884504247E-4 0.0 1.2049547256336164E-5 4 2.4099094512672328E-4 0.0 1.2049547256336164E-5 5 2.4099094512672328E-4 0.0 1.2049547256336164E-5 6 2.7713958689573175E-4 0.0 1.2049547256336164E-5 7 3.2533777592107645E-4 0.0 1.2049547256336164E-5 8 4.096846067154296E-4 0.0 1.2049547256336164E-5 9 4.699323429971104E-4 0.0 1.2049547256336164E-5 10-11 5.422296265351274E-4 3.6148641769008494E-5 1.2049547256336164E-5 12-13 6.62725099098489E-4 6.62725099098489E-5 1.2049547256336164E-5 14-15 7.048985144956656E-4 7.229728353801699E-5 1.2049547256336164E-5 16-17 8.314187606871953E-4 7.229728353801699E-5 1.2049547256336164E-5 18-19 0.0010904840266984228 8.434683079435315E-5 1.2049547256336164E-5 20-21 0.002235191016050358 8.434683079435315E-5 1.2049547256336164E-5 22-23 0.007404446789018573 1.0844592530702548E-4 1.2049547256336164E-5 24-25 0.013622013173288034 1.0844592530702548E-4 1.2049547256336164E-5 26-27 0.026286087339697343 1.0844592530702548E-4 1.2049547256336164E-5 28-29 0.05607256815736034 1.2652024619152973E-4 1.2049547256336164E-5 30-31 0.10480093726198378 1.686936615887063E-4 1.2049547256336164E-5 32-33 0.1737544714363675 1.686936615887063E-4 1.2049547256336164E-5 34-35 0.24829297076406298 1.8074320884504247E-4 1.2049547256336164E-5 36-37 0.34314700676594123 1.8074320884504247E-4 1.2049547256336164E-5 38-39 0.4662210824421589 1.8074320884504247E-4 1.2049547256336164E-5 40-41 0.6247148022783766 1.9279275610137862E-4 1.2049547256336164E-5 42-43 0.8190438756549381 1.9279275610137862E-4 1.2049547256336164E-5 44-45 1.0424003083720135 1.9279275610137862E-4 1.8074320884504247E-5 46-47 1.2942960937657209 1.9279275610137862E-4 2.4099094512672327E-5 48-49 1.5856842702920702 1.9279275610137862E-4 2.4099094512672327E-5 50-51 1.928451716319437 1.9279275610137862E-4 2.4099094512672327E-5 52-53 2.3324368871826193 1.9279275610137862E-4 2.4099094512672327E-5 54-55 2.8004955255813693 2.289413978703871E-4 2.4099094512672327E-5 56-57 3.343297505611173 2.289413978703871E-4 2.4099094512672327E-5 58-59 3.9668133779375436 2.4099094512672328E-4 2.4099094512672327E-5 60-61 4.682634807021078 2.4099094512672328E-4 2.4099094512672327E-5 62-63 5.497087805171352 2.4099094512672328E-4 2.4099094512672327E-5 64-65 6.403394502056677 2.4099094512672328E-4 2.4099094512672327E-5 66-67 7.383474552018919 2.4099094512672328E-4 2.4099094512672327E-5 68-69 8.45713138597387 2.470157187548914E-4 2.4099094512672327E-5 70-71 9.617526885853554 2.5304049238305946E-4 2.4099094512672327E-5 72-73 10.873945227818982 2.5304049238305946E-4 2.4099094512672327E-5 74-75 12.210372563566484 2.5906526601122753E-4 3.012386814084041E-5 76-77 13.613126632186486 2.650900396393956E-4 3.6148641769008494E-5 78-79 15.095998140513867 2.650900396393956E-4 3.6148641769008494E-5 80-81 16.664138270000713 2.650900396393956E-4 3.6148641769008494E-5 82-83 18.314582832021962 2.650900396393956E-4 4.8198189025344654E-5 84-85 20.038409136834296 2.650900396393956E-4 4.8198189025344654E-5 86-87 21.83573767991028 2.711148132675637E-4 4.8198189025344654E-5 88-89 23.688897800198504 2.831643605238998E-4 4.8198189025344654E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCCC 375 0.0 28.506935 44-45 GGGCGCC 1190 0.0 27.348843 42-43 GGGGCGC 1140 0.0 25.42262 42-43 TCGGGGG 1940 0.0 23.754347 38-39 GGGGGCG 1285 0.0 23.476915 40-41 GGGGGGC 1655 0.0 18.51536 40-41 GCGCCCG 565 0.0 17.238707 44-45 CGGGTAG 965 0.0 16.740278 14-15 CGGGGGG 2065 0.0 16.679806 38-39 CTCGGGG 1650 0.0 16.55616 36-37 GGGGCCC 535 0.0 15.541164 44-45 GGGCCCG 310 0.0 15.326309 44-45 GGGTAGA 2265 0.0 14.469351 28-29 GTAGGGG 1405 0.0 14.203688 18-19 GAGCGGC 1720 0.0 14.085348 9 AGGGTAG 2190 0.0 13.8804655 28-29 GGGCGGG 1075 0.0 13.478232 12-13 CGGGGGT 745 0.0 11.79746 38-39 CGAAAAT 5485 0.0 11.167755 1 GATCGGG 2955 0.0 11.087774 1 >>END_MODULE