FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n02 tsrexp_lib#35.3420000000d0f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n02 tsrexp_lib#35.3420000000d0f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8299067
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC3750.028.50693544-45
GGGCGCC11900.027.34884342-43
GGGGCGC11400.025.4226242-43
TCGGGGG19400.023.75434738-39
GGGGGCG12850.023.47691540-41
GGGGGGC16550.018.5153640-41
GCGCCCG5650.017.23870744-45
CGGGTAG9650.016.74027814-15
CGGGGGG20650.016.67980638-39
CTCGGGG16500.016.5561636-37
GGGGCCC5350.015.54116444-45
GGGCCCG3100.015.32630944-45
GGGTAGA22650.014.46935128-29
GTAGGGG14050.014.20368818-19
GAGCGGC17200.014.0853489
AGGGTAG21900.013.880465528-29
GGGCGGG10750.013.47823212-13
CGGGGGT7450.011.7974638-39
CGAAAAT54850.011.1677551
GATCGGG29550.011.0877741
Produced by FastQC (version 0.11.2)