##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l02n02 tsrexp_lib#32.3420000000d0e8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9726134 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.548960871811964 33.0 33.0 33.0 33.0 33.0 2 32.48394675623429 33.0 33.0 33.0 33.0 33.0 3 32.46901379314741 33.0 33.0 33.0 33.0 33.0 4 36.34194573095538 37.0 37.0 37.0 37.0 37.0 5 36.330652343469666 37.0 37.0 37.0 37.0 37.0 6 36.36431515337954 37.0 37.0 37.0 37.0 37.0 7 36.3686100767273 37.0 37.0 37.0 37.0 37.0 8 36.39522054703338 37.0 37.0 37.0 37.0 37.0 9 36.34413056616329 37.0 37.0 37.0 37.0 37.0 10-11 36.329338049424365 37.0 37.0 37.0 37.0 37.0 12-13 36.33029135728543 37.0 37.0 37.0 37.0 37.0 14-15 38.676294918412594 40.0 40.0 40.0 37.0 40.0 16-17 38.651757831014876 40.0 40.0 40.0 37.0 40.0 18-19 38.65507893475455 40.0 40.0 40.0 37.0 40.0 20-21 38.601207735776626 40.0 40.0 40.0 37.0 40.0 22-23 38.64550555236027 40.0 40.0 40.0 37.0 40.0 24-25 38.6035671521696 40.0 40.0 40.0 37.0 40.0 26-27 38.5850308046342 40.0 40.0 40.0 37.0 40.0 28-29 38.52864951274576 40.0 40.0 40.0 37.0 40.0 30-31 38.50883506231767 40.0 40.0 40.0 37.0 40.0 32-33 38.42951531410117 40.0 40.0 40.0 37.0 40.0 34-35 38.418126564984604 40.0 40.0 40.0 37.0 40.0 36-37 38.38493007601993 40.0 40.0 40.0 37.0 40.0 38-39 38.36737310014442 40.0 40.0 40.0 37.0 40.0 40-41 38.32500271947724 40.0 37.0 40.0 37.0 40.0 42-43 38.263981505909754 40.0 37.0 40.0 37.0 40.0 44-45 38.22312092348306 40.0 37.0 40.0 37.0 40.0 46-47 38.1269242743314 40.0 37.0 40.0 37.0 40.0 48-49 38.03375955955367 40.0 37.0 40.0 37.0 40.0 50-51 36.663194235242905 38.5 37.0 38.5 35.0 40.0 52-53 36.9854233963875 38.5 37.0 40.0 33.0 40.0 54-55 37.65021333245049 40.0 37.0 40.0 33.0 40.0 56-57 37.61271441458651 40.0 37.0 40.0 33.0 40.0 58-59 37.40735501896231 40.0 37.0 40.0 33.0 40.0 60-61 37.23056231797752 37.0 37.0 40.0 33.0 40.0 62-63 37.00211718242829 37.0 37.0 40.0 33.0 40.0 64-65 36.719170895650834 37.0 37.0 40.0 33.0 40.0 66-67 36.46149245938828 37.0 37.0 40.0 33.0 40.0 68-69 36.17155135843285 37.0 37.0 37.0 33.0 40.0 70-71 35.90739439740395 37.0 37.0 37.0 33.0 40.0 72-73 35.61222809597318 37.0 37.0 37.0 33.0 38.5 74-75 35.45817778163452 37.0 37.0 37.0 33.0 37.0 76-77 35.247690809112854 37.0 37.0 37.0 33.0 37.0 78-79 35.148352521155886 37.0 37.0 37.0 33.0 37.0 80-81 35.04777586860308 37.0 37.0 37.0 33.0 37.0 82-83 34.97265347156434 37.0 37.0 37.0 33.0 37.0 84-85 34.92562419970771 37.0 33.0 37.0 33.0 37.0 86-87 34.86418740477974 37.0 33.0 37.0 33.0 37.0 88-89 34.78081697208778 37.0 33.0 37.0 33.0 37.0 90-91 34.72013813504934 37.0 33.0 37.0 33.0 37.0 92-93 34.69375540168375 37.0 33.0 37.0 33.0 37.0 94-95 34.63210099716907 37.0 33.0 37.0 33.0 37.0 96-97 34.555813645997475 37.0 33.0 37.0 33.0 37.0 98-99 34.492279049414705 37.0 33.0 37.0 33.0 37.0 100-101 32.657658993799586 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1587344149685137 2306 2 0.17303934092497286 2306 3 0.16024644860505077 2306 4 0.19802541072863988 2306 5 0.2174929156072949 2306 6 0.2980997398046128 2306 7 0.23567139220176614 2306 8 0.270439100747744 2306 9 0.20840833021716776 2306 10-11 0.20750147874800007 2306 12-13 0.23299819001676525 2306 14-15 0.43531331403215745 2306 16-17 0.45347990511634606 2306 18-19 0.45352035824659964 2306 20-21 0.4892838818230274 2306 22-23 0.5194559015901987 2306 24-25 0.48734701744725584 2306 26-27 0.5277633470027894 2306 28-29 0.5335652056419136 2306 30-31 0.5527353845210214 2306 32-33 0.5513682190282125 2306 34-35 0.5718798870920523 2306 36-37 0.5752229904206914 2306 38-39 0.5261136111550826 2306 40-41 0.6203122524637621 2306 42-43 0.5766248456029146 2306 44-45 0.5916262790345499 2306 46-47 0.5634591023764415 2306 48-49 0.5923859227153656 2306 50-51 0.4929741226315798 2306 52-53 0.5985304418123931 2306 54-55 0.621309457906051 2306 56-57 0.5830803470695898 2306 58-59 0.5973791855503521 2306 60-61 0.6439877674969168 2306 62-63 0.6218719733949385 2306 64-65 0.6401869551365209 2306 66-67 0.6577694301528041 2306 68-69 0.6159386131304032 2306 70-71 0.638296133153581 2306 72-73 0.7166818960106767 2306 74-75 0.6416326732115962 2306 76-77 0.7343326603655811 2306 78-79 0.643030122210348 2306 80-81 0.6400126036321367 2306 82-83 0.6731579303428887 2306 84-85 0.6970135346090487 2306 86-87 0.6696969442247038 2306 88-89 0.7181724284493001 2306 90-91 0.7560067647659778 2306 92-93 0.7414392058581427 2306 94-95 0.7476944664201568 2306 96-97 0.7780827062810971 2306 98-99 0.7154748460247404 2306 100-101 0.9082983636896813 2307 1 0.19637627407175273 2307 2 0.23322725687343393 2307 3 0.25871381890626566 2307 4 0.2859137085171852 2307 5 0.30533954724977264 2307 6 0.28382949220340237 2307 7 0.29216384150297614 2307 8 0.3106174883493793 2307 9 0.34340599158927887 2307 10-11 0.32532022069113964 2307 12-13 0.31387810079580447 2307 14-15 0.5753116462998378 2307 16-17 0.5909216906655317 2307 18-19 0.5625089465030513 2307 20-21 0.6368731666157501 2307 22-23 0.616032677304112 2307 24-25 0.5530782148518938 2307 26-27 0.6254393049846243 2307 28-29 0.6167115483375483 2307 30-31 0.5885285596388954 2307 32-33 0.6809320608533156 2307 34-35 0.6509567456493457 2307 36-37 0.6846358997552571 2307 38-39 0.6224198285371969 2307 40-41 0.6777685912861244 2307 42-43 0.6055819207807005 2307 44-45 0.6292924750783939 2307 46-47 0.7093017770931311 2307 48-49 0.6364307059454717 2307 50-51 0.610813154412476 2307 52-53 0.6885327125358671 2307 54-55 0.6599874568228472 2307 56-57 0.644054052852276 2307 58-59 0.648266669029212 2307 60-61 0.6334890666440955 2307 62-63 0.6324103847468123 2307 64-65 0.7229950795460951 2307 66-67 0.6464990907448396 2307 68-69 0.6759733095678087 2307 70-71 0.6874533579383595 2307 72-73 0.7544029316561449 2307 74-75 0.7084202731057729 2307 76-77 0.7829869036035291 2307 78-79 0.7846905099825605 2307 80-81 0.7575094786711745 2307 82-83 0.7738967303252977 2307 84-85 0.8308193920977942 2307 86-87 0.8677399391242631 2307 88-89 0.8640938179582278 2307 90-91 0.8400427910433521 2307 92-93 0.8359821086793247 2307 94-95 0.8222599460996278 2307 96-97 0.7950414524506115 2307 98-99 0.9029056038977075 2307 100-101 0.8722165676669249 2201 1 0.21056340018387232 2201 2 0.23766122122074762 2201 3 0.24836088613126606 2201 4 0.28145399808546756 2201 5 0.32427193981742164 2201 6 0.2925150371394807 2201 7 0.2542171199238581 2201 8 0.22873089233410582 2201 9 0.2726773633058954 2201 10-11 0.27730407864792994 2201 12-13 0.2826721081597512 2201 14-15 0.5016570788833974 2201 16-17 0.5156035879264422 2201 18-19 0.5121546324600175 2201 20-21 0.5971980042110516 2201 22-23 0.5280114894042001 2201 24-25 0.5549688841984093 2201 26-27 0.5759646801468818 2201 28-29 0.6197931620156751 2201 30-31 0.5628920840147558 2201 32-33 0.6598020426572191 2201 34-35 0.6408872312214342 2201 36-37 0.6306079295474305 2201 38-39 0.6223291064769825 2201 40-41 0.5983094563827507 2201 42-43 0.5730617267830169 2201 44-45 0.628278417622333 2201 46-47 0.655772519815983 2201 48-49 0.6488534500740286 2201 50-51 0.5640716079842534 2201 52-53 0.704931394411517 2201 54-55 0.6613773930167213 2201 56-57 0.6620444112255583 2201 58-59 0.6765485783174867 2201 60-61 0.6747065275633659 2201 62-63 0.629101660535774 2201 64-65 0.5894878661791765 2201 66-67 0.6128868728183292 2201 68-69 0.6403491619957649 2201 70-71 0.6346723579848756 2201 72-73 0.7267224847266718 2201 74-75 0.7101136419386407 2201 76-77 0.7827544371333346 2201 78-79 0.743714606532258 2201 80-81 0.7556956952136034 2201 82-83 0.7429225464127427 2201 84-85 0.7160535428693109 2201 86-87 0.7830932031217017 2201 88-89 0.8469913001509113 2201 90-91 0.7508642743654761 2201 92-93 0.7995569196123924 2201 94-95 0.7624400935194515 2201 96-97 0.7300841989376536 2201 98-99 0.7535925028523138 2201 100-101 0.8288055685450217 1101 1 0.13477695830744807 1101 2 0.1682821972008739 1101 3 0.1607218871287941 1101 4 0.21499713339288462 1101 5 0.24576589117617686 1101 6 0.2676025341515995 1101 7 0.20592164745076502 1101 8 0.27779794231148713 1101 9 0.2835026126217741 1101 10-11 0.2744385601587709 1101 12-13 0.25750445204843686 1101 14-15 0.44159073984087627 1101 16-17 0.4317950410394431 1101 18-19 0.4776606313866054 1101 20-21 0.5073107256355556 1101 22-23 0.47194279299463204 1101 24-25 0.47839412652653834 1101 26-27 0.48473718391358034 1101 28-29 0.5308667383105004 1101 30-31 0.49208080097509566 1101 32-33 0.5859361946353658 1101 34-35 0.5635961516455268 1101 36-37 0.5466078735222482 1101 38-39 0.5249265275642117 1101 40-41 0.5463714624011544 1101 42-43 0.5354732966592692 1101 44-45 0.5085585019494658 1101 46-47 0.5032639848266456 1101 48-49 0.4981789315711751 1101 50-51 0.46489388519529484 1101 52-53 0.5216274819917999 1101 54-55 0.6126329495522 1101 56-57 0.6055552951166092 1101 58-59 0.6136563924598093 1101 60-61 0.6201737521422857 1101 62-63 0.5590785393762445 1101 64-65 0.573486213744296 1101 66-67 0.5829045915500828 1101 68-69 0.5977134252285197 1101 70-71 0.6078465336413501 1101 72-73 0.6956467890178786 1101 74-75 0.6046293543072281 1101 76-77 0.6386051201864404 1101 78-79 0.6122340211799795 1101 80-81 0.642317052288071 1101 82-83 0.6714225307903305 1101 84-85 0.6448134902241165 1101 86-87 0.6700613314144306 1101 88-89 0.6679693504408988 1101 90-91 0.7286162379786632 1101 92-93 0.6656386618845715 1101 94-95 0.6899370502287709 1101 96-97 0.6408914632482805 1101 98-99 0.6674496377278487 1101 100-101 0.6953586764878352 2304 1 0.17171483920860453 2304 2 0.23126618118008935 2304 3 0.23868574204806947 2304 4 0.25267372557893 2304 5 0.3039707133002665 2304 6 0.2722279573023556 2304 7 0.274286888979681 2304 8 0.3003874930083583 2304 9 0.2922845877709719 2304 10-11 0.29509754689615164 2304 12-13 0.30447380859068573 2304 14-15 0.4898683022964292 2304 16-17 0.4943936038662642 2304 18-19 0.5158342135642258 2304 20-21 0.5416167167579928 2304 22-23 0.4701569193488453 2304 24-25 0.4736961148021024 2304 26-27 0.5463918602595328 2304 28-29 0.5050599391449495 2304 30-31 0.4906333606422635 2304 32-33 0.572869730966012 2304 34-35 0.5666491021561626 2304 36-37 0.6067204455744744 2304 38-39 0.5973004076034343 2304 40-41 0.5857829785750113 2304 42-43 0.5817799886878348 2304 44-45 0.5793233128891373 2304 46-47 0.6120262448923697 2304 48-49 0.5539392445038089 2304 50-51 0.6030972246591517 2304 52-53 0.6365387698238862 2304 54-55 0.6505281300402288 2304 56-57 0.6170246758662401 2304 58-59 0.6099169561621309 2304 60-61 0.6254334187948771 2304 62-63 0.5722973148237926 2304 64-65 0.5999440773531077 2304 66-67 0.6076268261017006 2304 68-69 0.603936737092603 2304 70-71 0.6025089736072076 2304 72-73 0.6608154540414901 2304 74-75 0.6559916289712433 2304 76-77 0.7003867258121161 2304 78-79 0.6898778417402127 2304 80-81 0.7235318479210164 2304 82-83 0.7497046573951494 2304 84-85 0.7656222135576343 2304 86-87 0.7570822728486135 2304 88-89 0.8060805272614928 2304 90-91 0.8015485329842704 2304 92-93 0.7953894446962693 2304 94-95 0.7174469109600992 2304 96-97 0.7450445167594921 2304 98-99 0.7727982874964425 2304 100-101 0.7540068865415748 2202 1 0.15998614892978225 2202 2 0.16121338745433178 2202 3 0.1773516212662969 2202 4 0.1952076549652304 2202 5 0.20695810966178385 2202 6 0.2279365299639693 2202 7 0.17143175836162783 2202 8 0.219157343386442 2202 9 0.19351832546555414 2202 10-11 0.22758253250653127 2202 12-13 0.22683208712631853 2202 14-15 0.3393882761181928 2202 16-17 0.3436945221782466 2202 18-19 0.3752655123410662 2202 20-21 0.44524797842174735 2202 22-23 0.4386041572023984 2202 24-25 0.42732938498078 2202 26-27 0.4356556933283571 2202 28-29 0.4600594586418225 2202 30-31 0.4371339884957379 2202 32-33 0.471502441841416 2202 34-35 0.474981444069158 2202 36-37 0.4306541167095972 2202 38-39 0.4627529704506941 2202 40-41 0.49011456910197637 2202 42-43 0.45924972143448883 2202 44-45 0.4570123529505352 2202 46-47 0.4830703888508694 2202 48-49 0.5178743447061862 2202 50-51 0.4203375938893572 2202 52-53 0.5401256249409983 2202 54-55 0.5055431844224003 2202 56-57 0.5006309401102556 2202 58-59 0.49082284912119434 2202 60-61 0.517608724583873 2202 62-63 0.5538280277423127 2202 64-65 0.5677802734225637 2202 66-67 0.536278925367121 2202 68-69 0.5138491996136523 2202 70-71 0.5201425910989883 2202 72-73 0.5973785692232383 2202 74-75 0.5560420758746929 2202 76-77 0.5593966956298857 2202 78-79 0.5556550404389853 2202 80-81 0.5713306607287265 2202 82-83 0.5861270106375898 2202 84-85 0.5653205304695277 2202 86-87 0.5661513486983125 2202 88-89 0.5819089033943641 2202 90-91 0.6183139091731888 2202 92-93 0.612270828914987 2202 94-95 0.5746879212561353 2202 96-97 0.6426412131257138 2202 98-99 0.602725790772574 2202 100-101 0.7406670592075031 1102 1 0.07783580133074253 1102 2 0.15833057525154004 1102 3 0.13433524329364133 1102 4 0.1387450951741087 1102 5 0.12775405270776474 1102 6 0.1810780802972971 1102 7 0.16371685577052375 1102 8 0.1707120217565432 1102 9 0.1615080402651543 1102 10-11 0.16074904300880632 1102 12-13 0.16845526252202347 1102 14-15 0.15563539185824027 1102 16-17 0.20075917833489854 1102 18-19 0.2418085467602893 1102 20-21 0.26316900954685707 1102 22-23 0.21738285513793443 1102 24-25 0.21348495723425032 1102 26-27 0.29975114379054446 1102 28-29 0.2564223755426198 1102 30-31 0.3014203754302045 1102 32-33 0.36150946946180085 1102 34-35 0.2555242524605674 1102 36-37 0.2686052579985372 1102 38-39 0.29327295778529816 1102 40-41 0.327560444263888 1102 42-43 0.2401107015968691 1102 44-45 0.2787412665034097 1102 46-47 0.3152885024711338 1102 48-49 0.3435938360981936 1102 50-51 0.29281773360073515 1102 52-53 0.3719103701224924 1102 54-55 0.38109401025243983 1102 56-57 0.3714188559174332 1102 58-59 0.381521001065515 1102 60-61 0.39844019952084153 1102 62-63 0.39742098820134686 1102 64-65 0.4116510244540521 1102 66-67 0.3747792999567494 1102 68-69 0.38906275445720695 1102 70-71 0.33647346497307495 1102 72-73 0.4785882086273787 1102 74-75 0.4260376804937209 1102 76-77 0.480077453144645 1102 78-79 0.4148086050809283 1102 80-81 0.4144902750221249 1102 82-83 0.4016962434340101 1102 84-85 0.37311409955541563 1102 86-87 0.38389753569305896 1102 88-89 0.4248442383284541 1102 90-91 0.4187267729614561 1102 92-93 0.39613461780911763 1102 94-95 0.4831965761074102 1102 96-97 0.4339897340067296 1102 98-99 0.4322824203586251 1102 100-101 0.4844244332865344 2305 1 0.13468298122925404 2305 2 0.13332589935898653 2305 3 0.1519817115744928 2305 4 0.15533485065022035 2305 5 0.1720415647152791 2305 6 0.1860683796037037 2305 7 0.1592238483376036 2305 8 0.15299745795311281 2305 9 0.17454697092938432 2305 10-11 0.18017371236937407 2305 12-13 0.16822620215463502 2305 14-15 0.3036416587216024 2305 16-17 0.320477527471148 2305 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1213 64-65 -0.9143244630971523 1213 66-67 -1.0884882584548876 1213 68-69 -1.161092668742242 1213 70-71 -1.1108559378815954 1213 72-73 -0.9617081143175454 1213 74-75 -1.087777818876269 1213 76-77 -0.9837449170313022 1213 78-79 -1.0955053147659441 1213 80-81 -1.1271208279563822 1213 82-83 -1.0960563057449306 1213 84-85 -1.0783601011499186 1213 86-87 -1.1309462135296329 1213 88-89 -1.2356912443493968 1213 90-91 -1.3451919690341683 1213 92-93 -1.1941453172646206 1213 94-95 -1.1431789651841981 1213 96-97 -1.1952499857574566 1213 98-99 -1.297216652872926 1213 100-101 -1.40459518831668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12982.0 3 1039.0 4 662.0 5 514.0 6 538.0 7 1501.0 8 2777.0 9 5176.0 10 11554.0 11 17682.0 12 15117.0 13 11959.0 14 10544.0 15 10405.0 16 10244.0 17 10954.0 18 12320.0 19 13418.0 20 15052.0 21 16827.0 22 19735.0 23 21915.0 24 25167.0 25 28471.0 26 33307.0 27 39640.0 28 48241.0 29 59660.0 30 75855.0 31 98198.0 32 131131.0 33 180670.0 34 257023.0 35 389034.0 36 790158.0 37 3573439.0 38 3771788.0 39 1437.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.978557774279505 14.18808043429858 19.566514997755966 38.26684679366595 2 25.511411774930217 24.94538460809749 30.17941115051895 19.36379246645334 3 23.55707322116425 27.645399657073295 29.417987412977276 19.37953970878518 4 25.686814841631865 31.56044591494329 21.185438848867527 21.567300394557314 5 27.161625734778998 34.14749775011427 21.435700027046007 17.255176488060723 6 19.170182377612583 37.59107296031271 22.623175168388567 20.615569493686138 7 18.089928171271865 16.0268074909147 43.52726393126487 22.356000406548564 8 22.08490934370974 21.61594672645622 27.904835606192897 28.394308323641145 9 24.259912234094323 23.28625632138936 26.962943112619524 25.490888331896794 10-11 24.352271162958452 31.53433681765676 21.722498107823238 22.390893911561545 12-13 22.977938148490402 25.606450230034444 28.02635203917751 23.38925958229764 14-15 23.074167103653117 26.935570127092877 27.80037837421056 22.189884395043443 16-17 24.026509392958552 27.268997029844442 26.817879736875806 21.886613840321196 18-19 23.875936462104573 27.5320425266997 26.90463918269102 21.687381828504705 20-21 23.940106752845995 27.777877954884033 26.681750934618055 21.600264357651916 22-23 24.146956736833225 27.558887685465855 26.71541783396011 21.57873774374081 24-25 23.519860560416095 28.10025928592841 26.72170698114743 21.658173172508068 26-27 23.78717865975392 28.213788707165406 26.51942886212127 21.4796037709594 28-29 23.93732027437897 27.60566996024995 26.801747741202934 21.655262024168145 30-31 23.762236422281173 27.605199807315394 26.861005198129025 21.77155857227441 32-33 23.805439247591174 27.82987790968025 26.7077324899082 21.656950352820374 34-35 23.914743674285063 27.697702554118948 26.715854183621097 21.671699587974896 36-37 23.461908739096252 27.66000136163263 27.075972200034755 21.802117699236362 38-39 23.771089987913818 27.638019715789042 26.806823613154386 21.784066683142754 40-41 24.290293350480987 27.4083274273218 26.69304791048257 21.608331311714643 42-43 24.05000560933616 27.398743240852948 26.922969546507066 21.62828160330383 44-45 24.27935486286533 27.518523510305183 26.654727350624945 21.547394276204543 46-47 24.2151074621065 27.395586766159564 26.682058630134136 21.707247141599797 48-49 23.815381086399317 27.42460918796451 26.84408000845403 21.91592971718215 50-51 24.383430957773804 27.582372070429713 26.58384171705001 21.450355254746476 52-53 24.330202188723586 27.507070294700277 26.74764795970098 21.41507955687516 54-55 24.11895985580349 27.5998301753276 26.62064553970306 21.660564429165856 56-57 24.353984484208276 27.54470830959237 26.748376257048346 21.352930949151013 58-59 24.57304259007784 27.77620667630474 26.439959127102885 21.210791606514533 60-61 24.373122869925602 27.967120584802124 26.391240119724408 21.268516425547865 62-63 24.72691935917749 28.007784394913887 26.063276524926664 21.20201972098196 64-65 24.885510240275554 27.949255798538395 26.117223960364182 21.04801000082187 66-67 24.7305799997765 28.035185876870955 26.175271351151043 21.0589627722015 68-69 25.170825965794013 27.979540026571552 25.903516291403715 20.94611771623071 70-71 25.39946285827042 27.9238878943727 25.803915462073096 20.872733785283785 72-73 25.35264172758153 28.026347006762357 25.87858074125328 20.742430524402828 74-75 25.74698138954346 28.065933405862992 25.597325218556172 20.58975998603738 76-77 26.025692845466352 27.893351358916384 25.56813977508145 20.51281602053581 78-79 25.99792601281528 27.991578136498607 25.556575192648555 20.453920658037557 80-81 26.484227444472737 28.004925718900154 25.165091573550136 20.345755263076974 82-83 26.726916535087952 27.87346384513573 25.234488507236247 20.165131112540067 84-85 26.70496172991269 27.969341496808408 25.221705211746766 20.103991561532133 86-87 27.079555584299175 28.054178791164247 24.91112592231314 19.955139702223438 88-89 27.4701651251505 27.957630198632778 24.90170729665174 19.67049737956498 90-91 27.470259481099603 28.05040553111158 24.878620820853207 19.60071416693562 92-93 27.869994469315618 28.088024232646507 24.60734895673147 19.434632341306408 94-95 28.223598405086932 28.02630151510151 24.531995908351142 19.218104171460414 96-97 28.145523889203933 28.179240822163827 24.513811661681988 19.161423626950253 98-99 28.59914617226618 28.222565891509188 24.305885894027927 18.8724020421967 100-101 28.818197644449413 28.214838628920585 24.150502852386325 18.816460874243678 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11200.0 1 6134.5 2 837.0 3 527.5 4 423.0 5 379.0 6 356.5 7 366.5 8 382.5 9 372.5 10 381.0 11 395.0 12 396.5 13 412.5 14 462.0 15 523.5 16 585.0 17 665.0 18 743.5 19 853.5 20 1005.5 21 1219.0 22 1557.0 23 2136.5 24 3128.0 25 4552.5 26 6693.0 27 9659.5 28 13743.5 29 19652.0 30 26379.5 31 34115.5 32 43493.0 33 54396.5 34 68027.0 35 86573.5 36 113370.0 37 146495.0 38 187433.5 39 238364.5 40 298957.0 41 367906.0 42 442353.0 43 515750.0 44 582493.5 45 637830.0 46 675543.0 47 691881.0 48 682114.0 49 650289.0 50 597394.5 51 526171.0 52 445983.5 53 368980.0 54 293726.0 55 224738.5 56 170671.0 57 127842.5 58 95169.5 59 69863.5 60 49802.5 61 34312.5 62 24465.5 63 17842.0 64 12651.0 65 9495.0 66 7277.5 67 5271.0 68 4204.0 69 3368.0 70 2691.0 71 1968.5 72 1148.0 73 713.0 74 647.5 75 564.5 76 483.0 77 260.0 78 101.5 79 60.5 80 50.0 81 47.0 82 46.0 83 36.5 84 31.5 85 27.5 86 23.5 87 22.0 88 16.5 89 12.0 90 9.0 91 8.0 92 6.5 93 7.0 94 6.5 95 4.5 96 2.0 97 0.5 98 0.5 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16397059715607454 2 0.14996708867058586 3 0.1544395748608851 4 0.1463788181408975 5 0.17223698542504143 6 0.15385352494629417 7 0.15542660629598565 8 0.14235872135835265 9 0.1690394148384137 10-11 0.14162872935947624 12-13 0.13166588081143032 14-15 0.1907078393121049 16-17 0.19776614223081854 18-19 0.18286813650727 20-21 0.216941284173136 22-23 0.20113849963407865 24-25 0.19230662460541875 26-27 0.17792269775431843 28-29 0.1951032136715369 30-31 0.1428059699773826 32-33 0.16984137787943288 34-35 0.17371239178896775 36-37 0.17685341369962618 38-39 0.17595377567284184 40-41 0.2120832388285006 42-43 0.1964089740075553 44-45 0.18088893284834448 46-47 0.1854539532356844 48-49 0.2250071816818481 50-51 0.20274242571611703 52-53 0.1677902031783646 54-55 0.15362733024241698 56-57 0.14954040320645387 58-59 0.16483424966178753 60-61 0.15935416888148982 62-63 0.14801872974400723 64-65 0.1699750383862694 66-67 0.17373295494386568 68-69 0.18515064670093997 70-71 0.1667671862221927 72-73 0.17502843370243512 74-75 0.2078883552293234 76-77 0.23532474465188324 78-79 0.217517052510278 80-81 0.19245570747842874 82-83 0.1629630025660761 84-85 0.1726122630019286 86-87 0.16209935006036316 88-89 0.16558480481556187 90-91 0.17683799133345274 92-93 0.16206336453929177 94-95 0.15560653390134252 96-97 0.16326630910082052 98-99 0.1534474026370601 100-101 0.16019211744358036 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9726134.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.191851930951124 #Duplication Level Percentage of deduplicated Percentage of total 1 70.45066661529518 33.951480939611066 2 14.760592077263949 14.22680535601349 3 5.4204754082270314 7.836682448059169 4 2.6941741016963214 5.193489575406095 5 1.635229420910006 3.9402367062814982 6 1.161133061491136 3.357429154290767 7 0.7338453247330966 2.47557556608307 8 0.4971188397162371 1.91656620125529 9 0.4076198187566461 1.7679558554677282 >10 2.0906548940670713 18.103758104180862 >50 0.10626351912196443 3.4732549301703544 >100 0.04115409961314636 3.301238514793217 >500 9.650911569397124E-4 0.33038730154738843 >1k 8.606639430340892E-5 0.07139883173886194 >5k 2.166155704593205E-5 0.05374051510120249 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.7478681663238445E-4 0.0 0.0 2 1.7478681663238445E-4 0.0 0.0 3 2.0563154897927584E-4 0.0 0.0 4 3.0844732346891376E-4 0.0 0.0 5 3.2901047836684133E-4 0.0 1.0281577448963792E-5 6 3.701367881626965E-4 0.0 1.0281577448963792E-5 7 4.112630979585517E-4 0.0 1.0281577448963792E-5 8 4.729525626523344E-4 0.0 1.0281577448963792E-5 9 5.140788724481896E-4 0.0 1.0281577448963792E-5 10-11 6.42598590560237E-4 0.0 1.0281577448963792E-5 12-13 7.608367312233206E-4 0.0 1.0281577448963792E-5 14-15 8.585117169884766E-4 1.0281577448963792E-5 1.0281577448963792E-5 16-17 0.0010127353787229335 2.0563154897927583E-5 1.0281577448963792E-5 18-19 0.001285197181120474 3.084473234689137E-5 1.0281577448963792E-5 20-21 0.0022927917711189254 3.084473234689137E-5 1.0281577448963792E-5 22-23 0.007896251480804192 3.084473234689137E-5 1.0281577448963792E-5 24-25 0.015057370174007472 3.084473234689137E-5 1.0281577448963792E-5 26-27 0.028515955054701076 4.112630979585517E-5 1.0281577448963792E-5 28-29 0.05940181371138831 6.168946469378275E-5 1.0281577448963792E-5 30-31 0.10809022372095634 7.711183086722844E-5 1.0281577448963792E-5 32-33 0.174067106210957 8.739340831619223E-5 1.0281577448963792E-5 34-35 0.245904487846867 1.0281577448963792E-4 1.0281577448963792E-5 36-37 0.33826389807090873 1.182381406630836E-4 1.0281577448963792E-5 38-39 0.45568465332679975 1.3366050683652928E-4 1.0281577448963792E-5 40-41 0.6027934634665737 1.6964602790790257E-4 1.0281577448963792E-5 42-43 0.7883759364203702 1.7478681663238445E-4 2.0563154897927583E-5 44-45 0.9984902531673941 1.7992760535686633E-4 2.0563154897927583E-5 46-47 1.2395572588245236 1.8506839408134824E-4 2.0563154897927583E-5 48-49 1.513437918910021 1.8506839408134824E-4 2.0563154897927583E-5 50-51 1.8385979465222255 1.8506839408134824E-4 2.0563154897927583E-5 52-53 2.2170473900524095 1.9020918280583015E-4 2.0563154897927583E-5 54-55 2.641691961060787 1.9534997153031205E-4 2.0563154897927583E-5 56-57 3.1208083293937756 1.9534997153031205E-4 2.0563154897927583E-5 58-59 3.661269729576006 2.0563154897927584E-4 2.0563154897927583E-5 60-61 4.269923692188489 2.0563154897927584E-4 2.0563154897927583E-5 62-63 4.9527438137290725 2.0563154897927584E-4 2.0563154897927583E-5 64-65 5.70348403589751 2.0563154897927584E-4 2.0563154897927583E-5 66-67 6.522884632270129 2.0563154897927584E-4 2.0563154897927583E-5 68-69 7.4147703496579425 2.0563154897927584E-4 2.0563154897927583E-5 70-71 8.38241072968972 2.0563154897927584E-4 2.0563154897927583E-5 72-73 9.428720599572245 2.1591312642823963E-4 2.0563154897927583E-5 74-75 10.54974669277639 2.2105391515272153E-4 2.0563154897927583E-5 76-77 11.731865919182276 2.261947038772034E-4 2.0563154897927583E-5 78-79 12.987945672967285 2.261947038772034E-4 2.0563154897927583E-5 80-81 14.328082463186298 2.261947038772034E-4 2.0563154897927583E-5 82-83 15.739496289070251 2.261947038772034E-4 2.0563154897927583E-5 84-85 17.211807898184418 2.364762813261672E-4 2.0563154897927583E-5 86-87 18.748363943988434 2.364762813261672E-4 2.0563154897927583E-5 88-89 20.347077266260158 2.416170700506491E-4 2.0563154897927583E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGCC 1850 0.0 31.33251 42-43 GGCGCCC 575 0.0 30.573439 44-45 GGGGCGC 1815 0.0 28.140957 42-43 TCGGGGG 3025 0.0 27.171688 38-39 GGGGGCG 2200 0.0 23.97118 40-41 CGGGTAG 1265 0.0 22.72321 14-15 CTCGGGG 2625 0.0 22.172285 36-37 GGGCCCG 490 0.0 21.817093 44-45 GTAGGGG 2020 0.0 20.581665 18-19 GAGCGGC 2305 0.0 20.196808 9 GGGGGGC 2685 0.0 19.81814 40-41 GATCGGG 3380 0.0 18.965853 1 GGGCGGG 1645 0.0 18.337868 12-13 GGGTAGA 3005 0.0 18.255255 28-29 GGGGCCC 905 0.0 17.587603 44-45 AGGGTAG 2970 0.0 17.510883 28-29 CGGGGGT 1360 0.0 17.467348 38-39 GGGGGTC 1415 0.0 17.459671 40-41 GCGCCCG 825 0.0 17.277374 44-45 CGGGGGG 3230 0.0 16.695107 38-39 >>END_MODULE