FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n02 tsrexp_lib#32.3420000000d0e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n02 tsrexp_lib#32.3420000000d0e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9726134
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC18500.031.3325142-43
GGCGCCC5750.030.57343944-45
GGGGCGC18150.028.14095742-43
TCGGGGG30250.027.17168838-39
GGGGGCG22000.023.9711840-41
CGGGTAG12650.022.7232114-15
CTCGGGG26250.022.17228536-37
GGGCCCG4900.021.81709344-45
GTAGGGG20200.020.58166518-19
GAGCGGC23050.020.1968089
GGGGGGC26850.019.8181440-41
GATCGGG33800.018.9658531
GGGCGGG16450.018.33786812-13
GGGTAGA30050.018.25525528-29
GGGGCCC9050.017.58760344-45
AGGGTAG29700.017.51088328-29
CGGGGGT13600.017.46734838-39
GGGGGTC14150.017.45967140-41
GCGCCCG8250.017.27737444-45
CGGGGGG32300.016.69510738-39
Produced by FastQC (version 0.11.2)