##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l02n02 tsrexp_lib#29.3420000000d0db.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5909014 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.534867069192934 33.0 33.0 33.0 33.0 33.0 2 32.4584324897521 33.0 33.0 33.0 33.0 33.0 3 32.43759212619906 33.0 33.0 33.0 33.0 33.0 4 36.30508084766765 37.0 37.0 37.0 37.0 37.0 5 36.296641030127866 37.0 37.0 37.0 37.0 37.0 6 36.35032511346225 37.0 37.0 37.0 37.0 37.0 7 36.32867835479828 37.0 37.0 37.0 37.0 37.0 8 36.375128743983346 37.0 37.0 37.0 37.0 37.0 9 36.30095494781363 37.0 37.0 37.0 37.0 37.0 10-11 36.286858518189334 37.0 37.0 37.0 37.0 37.0 12-13 36.28975248662467 37.0 37.0 37.0 37.0 37.0 14-15 38.62741237370567 40.0 40.0 40.0 37.0 40.0 16-17 38.60136758856892 40.0 40.0 40.0 37.0 40.0 18-19 38.60695075354366 40.0 40.0 40.0 37.0 40.0 20-21 38.554512309498676 40.0 40.0 40.0 37.0 40.0 22-23 38.62361461658409 40.0 40.0 40.0 37.0 40.0 24-25 38.562069323240735 40.0 40.0 40.0 37.0 40.0 26-27 38.55134460673135 40.0 40.0 40.0 37.0 40.0 28-29 38.48136101894495 40.0 40.0 40.0 37.0 40.0 30-31 38.45637470143073 40.0 40.0 40.0 37.0 40.0 32-33 38.37985457472262 40.0 40.0 40.0 37.0 40.0 34-35 38.37107646047208 40.0 40.0 40.0 37.0 40.0 36-37 38.33958956604266 40.0 40.0 40.0 37.0 40.0 38-39 38.32593568063979 40.0 40.0 40.0 37.0 40.0 40-41 38.28872101166117 40.0 37.0 40.0 37.0 40.0 42-43 38.214866473492876 40.0 37.0 40.0 37.0 40.0 44-45 38.182153486182294 40.0 37.0 40.0 37.0 40.0 46-47 38.07284260961304 40.0 37.0 40.0 37.0 40.0 48-49 37.98932469951839 40.0 37.0 40.0 37.0 40.0 50-51 36.60692900710677 38.5 37.0 38.5 35.0 40.0 52-53 36.930883646577925 38.5 37.0 40.0 33.0 40.0 54-55 37.591970758573254 40.0 37.0 40.0 33.0 40.0 56-57 37.56826088074931 40.0 37.0 40.0 33.0 40.0 58-59 37.353332552605224 40.0 37.0 40.0 33.0 40.0 60-61 37.19119898852837 37.0 37.0 40.0 33.0 40.0 62-63 36.95973896829488 37.0 37.0 40.0 33.0 40.0 64-65 36.66949680606612 37.0 37.0 40.0 33.0 40.0 66-67 36.400621998864786 37.0 37.0 40.0 33.0 40.0 68-69 36.09512788427985 37.0 37.0 37.0 33.0 40.0 70-71 35.818485114437024 37.0 37.0 37.0 33.0 40.0 72-73 35.52089206422595 37.0 37.0 37.0 33.0 38.5 74-75 35.36835917125937 37.0 37.0 37.0 33.0 37.0 76-77 35.16101781109336 37.0 37.0 37.0 33.0 37.0 78-79 35.06199604536391 37.0 37.0 37.0 33.0 37.0 80-81 34.95998655613271 37.0 37.0 37.0 33.0 37.0 82-83 34.8826563111883 37.0 33.0 37.0 33.0 37.0 84-85 34.83543658891314 37.0 33.0 37.0 33.0 37.0 86-87 34.77579694344945 37.0 33.0 37.0 33.0 37.0 88-89 34.69226710243029 37.0 33.0 37.0 33.0 37.0 90-91 34.63050282162134 37.0 33.0 37.0 33.0 37.0 92-93 34.60108480027294 37.0 33.0 37.0 33.0 37.0 94-95 34.536012437946496 37.0 33.0 37.0 33.0 37.0 96-97 34.45487673578029 37.0 33.0 37.0 33.0 37.0 98-99 34.383811918536665 37.0 33.0 37.0 33.0 37.0 100-101 32.51463695973643 35.0 30.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15036434545260846 2306 2 0.20072724060358382 2306 3 0.2091548285911955 2306 4 0.24187825298540844 2306 5 0.27619940529442744 2306 6 0.304093096487037 2306 7 0.23266936247731707 2306 8 0.24725870954346618 2306 9 0.2143388204778276 2306 10-11 0.22593178601514552 2306 12-13 0.24931848422264835 2306 14-15 0.5006508450714975 2306 16-17 0.47671882210015326 2306 18-19 0.44715945481984676 2306 20-21 0.5591477558895903 2306 22-23 0.5482240925427817 2306 24-25 0.5011889761123811 2306 26-27 0.4948331004123503 2306 28-29 0.5421170025605875 2306 30-31 0.5566601404212506 2306 32-33 0.5650516790197244 2306 34-35 0.5263881193809326 2306 36-37 0.5701678219274484 2306 38-39 0.5670294469500021 2306 40-41 0.6019039041693119 2306 42-43 0.551738149050685 2306 44-45 0.5699041603360442 2306 46-47 0.626169352904391 2306 48-49 0.6124027648155561 2306 50-51 0.5611570361094209 2306 52-53 0.629471230136808 2306 54-55 0.6850435833840933 2306 56-57 0.590187915684723 2306 58-59 0.6473570223298921 2306 60-61 0.5409213016445662 2306 62-63 0.5606965980083771 2306 64-65 0.6420717228816173 2306 66-67 0.5848590334283799 2306 68-69 0.567150089654497 2306 70-71 0.648950012463203 2306 72-73 0.6595847134325368 2306 74-75 0.6613265388760468 2306 76-77 0.730461134240386 2306 78-79 0.7683352973055619 2306 80-81 0.7516144930813624 2306 82-83 0.7345795873052836 2306 84-85 0.7308968468042067 2306 86-87 0.6952010321722355 2306 88-89 0.7804580438775659 2306 90-91 0.8052440319054952 2306 92-93 0.786863218592849 2306 94-95 0.7673357634753728 2306 96-97 0.7336924141286758 2306 98-99 0.6221936462267408 2306 100-101 0.8320520317851887 2307 1 0.16269764694344957 2307 2 0.20914774275968284 2307 3 0.23685274498995312 2307 4 0.2752406119752209 2307 5 0.25850125620053177 2307 6 0.3066336068731985 2307 7 0.22835103063201245 2307 8 0.234983275658891 2307 9 0.27577125499557553 2307 10-11 0.32657350408906183 2307 12-13 0.27782983852059573 2307 14-15 0.48792724341618054 2307 16-17 0.45703434445787394 2307 18-19 0.4863352884501779 2307 20-21 0.5926785892198652 2307 22-23 0.5674358316449926 2307 24-25 0.5218973123544046 2307 26-27 0.5705240059515972 2307 28-29 0.5650565330552837 2307 30-31 0.5279489285572225 2307 32-33 0.6223250706983023 2307 34-35 0.5157932955005364 2307 36-37 0.5969433776707902 2307 38-39 0.5876528188494916 2307 40-41 0.6135349868507944 2307 42-43 0.5244736273432906 2307 44-45 0.5521693464492898 2307 46-47 0.5483211950538092 2307 48-49 0.5394458740389823 2307 50-51 0.4770575693486947 2307 52-53 0.5737535279155495 2307 54-55 0.6257684011776732 2307 56-57 0.6320075181679456 2307 58-59 0.6375590262024247 2307 60-61 0.5490265937443226 2307 62-63 0.6041958919623482 2307 64-65 0.6121935908357017 2307 66-67 0.5636934971701848 2307 68-69 0.5835674204208416 2307 70-71 0.5052333628812562 2307 72-73 0.6510180113717183 2307 74-75 0.6171234952236659 2307 76-77 0.6677236933480089 2307 78-79 0.6073672120558697 2307 80-81 0.6527136254959132 2307 82-83 0.6623403007730602 2307 84-85 0.65643838349839 2307 86-87 0.7637031888887336 2307 88-89 0.7335258843074897 2307 90-91 0.7131331930885167 2307 92-93 0.7465508529283582 2307 94-95 0.7338706052855457 2307 96-97 0.7596732853637675 2307 98-99 0.8449827821807077 2307 100-101 0.874382874668207 2201 1 0.2038607242297914 2201 2 0.22449624855148897 2201 3 0.21931474854433475 2201 4 0.2507026447837504 2201 5 0.28846913409368824 2201 6 0.32000628182088064 2201 7 0.25253481359125374 2201 8 0.2684081065855466 2201 9 0.2502895801116267 2201 10-11 0.262483266513982 2201 12-13 0.3008894913991469 2201 14-15 0.5295156160388927 2201 16-17 0.5378429397464899 2201 18-19 0.5201602091244055 2201 20-21 0.620890107729906 2201 22-23 0.5857920302347353 2201 24-25 0.5309564360461962 2201 26-27 0.6138802959605698 2201 28-29 0.6233606514134138 2201 30-31 0.626009639286913 2201 32-33 0.6789139030291551 2201 34-35 0.6483534049528714 2201 36-37 0.6470434686721163 2201 38-39 0.6511070397538816 2201 40-41 0.6232599037637669 2201 42-43 0.6329443768451881 2201 44-45 0.6318641289707756 2201 46-47 0.566628549652826 2201 48-49 0.5659912117102905 2201 50-51 0.5059859452520499 2201 52-53 0.6852781369285381 2201 54-55 0.688151515157081 2201 56-57 0.6422417708131007 2201 58-59 0.6466792701820765 2201 60-61 0.631987328399731 2201 62-63 0.6295728265239404 2201 64-65 0.555707409244711 2201 66-67 0.5875141498721703 2201 68-69 0.6170420748300174 2201 70-71 0.5654719775298673 2201 72-73 0.6919133609014025 2201 74-75 0.5903469321898456 2201 76-77 0.6845194309131699 2201 78-79 0.7045542161065228 2201 80-81 0.6995532495173435 2201 82-83 0.6785322142977819 2201 84-85 0.7039818703220106 2201 86-87 0.781949749697155 2201 88-89 0.8274343334298138 2201 90-91 0.816954360350195 2201 92-93 0.7658431191745976 2201 94-95 0.6995137552684625 2201 96-97 0.7208142339715238 2201 98-99 0.7415835146838461 2201 100-101 0.8027540111607792 1101 1 0.12634529726949495 1101 2 0.1478890966524844 1101 3 0.1838817207920158 1101 4 0.2018334998211202 1101 5 0.23214209348630277 1101 6 0.23678477969635026 1101 7 0.17718723330345654 1101 8 0.2219403554461934 1101 9 0.22407440034916704 1101 10-11 0.21167272020122851 1101 12-13 0.2140983717856031 1101 14-15 0.43695797440106077 1101 16-17 0.4432774865311089 1101 18-19 0.40844031651271706 1101 20-21 0.47323984252811613 1101 22-23 0.475569213892463 1101 24-25 0.481209049708788 1101 26-27 0.44988763935955944 1101 28-29 0.5035189235201329 1101 30-31 0.44993937477253354 1101 32-33 0.4840716086159702 1101 34-35 0.4820596475250909 1101 36-37 0.46607744219280534 1101 38-39 0.5210877412128525 1101 40-41 0.5089514218061524 1101 42-43 0.49879273578612526 1101 44-45 0.4788303525664688 1101 46-47 0.45085589837113815 1101 48-49 0.48614481287399514 1101 50-51 0.42901611219271985 1101 52-53 0.4675180796281708 1101 54-55 0.5364745674643885 1101 56-57 0.5093723079487731 1101 58-59 0.5880400151996596 1101 60-61 0.6223401721724073 1101 62-63 0.5819433572105055 1101 64-65 0.5871953984265161 1101 66-67 0.5422639014979254 1101 68-69 0.594637215748179 1101 70-71 0.6006349731656542 1101 72-73 0.6378923544519637 1101 74-75 0.58495116897803 1101 76-77 0.5919637409228358 1101 78-79 0.602269563331177 1101 80-81 0.6617045700725086 1101 82-83 0.6708714015255097 1101 84-85 0.6897366325858627 1101 86-87 0.7403217590819722 1101 88-89 0.6718596204060177 1101 90-91 0.7142441942075237 1101 92-93 0.639215079226112 1101 94-95 0.6451487709472445 1101 96-97 0.6825154829283449 1101 98-99 0.6147607978972829 1101 100-101 0.6890210483657953 2304 1 0.12926449480072932 2304 2 0.11766014871410846 2304 3 0.08180188590596771 2304 4 0.11666466632693329 2304 5 0.13018421089194732 2304 6 0.16889970564530898 2304 7 0.06683449528070895 2304 8 0.19239084248488325 2304 9 0.0771495880966171 2304 10-11 0.13685593461216428 2304 12-13 0.13370831919768733 2304 14-15 0.295549376272902 2304 16-17 0.3268292137300719 2304 18-19 0.319023147312798 2304 20-21 0.33348190175590275 2304 22-23 0.4123655007193392 2304 24-25 0.31330059674167643 2304 26-27 0.40186386679283714 2304 28-29 0.3704161745547623 2304 30-31 0.3035984682018906 2304 32-33 0.4165707315521061 2304 34-35 0.4125915098301718 2304 36-37 0.4681511511032781 2304 38-39 0.4611879897780966 2304 40-41 0.5000325969384392 2304 42-43 0.42614424303609155 2304 44-45 0.4573672691795565 2304 46-47 0.46261502992712167 2304 48-49 0.3945912569578809 2304 50-51 0.4226489735895953 2304 52-53 0.44588279771465267 2304 54-55 0.4411334947928225 2304 56-57 0.4639511769064981 2304 58-59 0.4847214664546158 2304 60-61 0.49850263039784437 2304 62-63 0.46424185917312855 2304 64-65 0.5425552820027306 2304 66-67 0.5395933353830955 2304 68-69 0.43446247607614197 2304 70-71 0.4241420242297238 2304 72-73 0.4797372014524157 2304 74-75 0.4179501261441345 2304 76-77 0.49782999593907107 2304 78-79 0.5315541089411937 2304 80-81 0.53303644130186 2304 82-83 0.5730957262430536 2304 84-85 0.5554246179311733 2304 86-87 0.5700377889166148 2304 88-89 0.6623463962871128 2304 90-91 0.6286137854697671 2304 92-93 0.5603062742990161 2304 94-95 0.5445088239331426 2304 96-97 0.5850808959160005 2304 98-99 0.5636319214569809 2304 100-101 0.6184653136632647 2202 1 0.15067948499631 2202 2 0.1676664800105172 2202 3 0.17118284711909126 2202 4 0.20408284299953294 2202 5 0.22890069345508834 2202 6 0.2577535822997561 2202 7 0.2080176939045515 2202 8 0.21935885902362173 2202 9 0.2664212972523927 2202 10-11 0.2766726973062461 2202 12-13 0.23563008255796802 2202 14-15 0.4202478131535159 2202 16-17 0.4012727374089522 2202 18-19 0.4208832507889966 2202 20-21 0.44825636213273157 2202 22-23 0.4054135967513872 2202 24-25 0.4111373514546912 2202 26-27 0.49618361114731613 2202 28-29 0.53831488174702 2202 30-31 0.45704826432753265 2202 32-33 0.5059849610002729 2202 34-35 0.5047136083028292 2202 36-37 0.5129698280640014 2202 38-39 0.5430107799324873 2202 40-41 0.5395329435893075 2202 42-43 0.5018259236365736 2202 44-45 0.5121672845201388 2202 46-47 0.5044012556049466 2202 48-49 0.4976767473126529 2202 50-51 0.4197510003648617 2202 52-53 0.5421637975106606 2202 54-55 0.5519358518865332 2202 56-57 0.5256293688469427 2202 58-59 0.515721923696546 2202 60-61 0.5391823669762346 2202 62-63 0.5605126925271264 2202 64-65 0.569407500287312 2202 66-67 0.5248369514360931 2202 68-69 0.5168654973112794 2202 70-71 0.5018672423288351 2202 72-73 0.5987188330886681 2202 74-75 0.5015574574878201 2202 76-77 0.5963370332659395 2202 78-79 0.5881738760751176 2202 80-81 0.6127556871535376 2202 82-83 0.6606406941518088 2202 84-85 0.5960043431694331 2202 86-87 0.6422141960265719 2202 88-89 0.6694028575651956 2202 90-91 0.7058332850692324 2202 92-93 0.6564757325218764 2202 94-95 0.6236458550080783 2202 96-97 0.6301480721280441 2202 98-99 0.58083374797814 2202 100-101 0.6887131549887471 1102 1 0.04526782726661338 1102 2 0.0783948541062287 1102 3 0.10338334723275011 1102 4 0.14176921557083944 1102 5 0.08945055104304345 1102 6 0.12740978758705523 1102 7 0.07728945352906891 1102 8 0.12985990872742548 1102 9 0.10851107701517293 1102 10-11 0.09990458155801463 1102 12-13 0.10657501656703516 1102 14-15 0.1341868099843424 1102 16-17 0.14618301113426924 1102 18-19 0.21314720115204722 1102 20-21 0.1966600768807396 1102 22-23 0.13556450003153486 1102 24-25 0.17591845143730467 1102 26-27 0.25272420192330003 1102 28-29 0.16395172434494043 1102 30-31 0.24921359602056725 1102 32-33 0.2748411850084054 1102 34-35 0.18110062139404448 1102 36-37 0.21742304604135398 1102 38-39 0.2762238764918621 1102 40-41 0.31794514957195474 1102 42-43 0.2771575498082939 1102 44-45 0.3138167235637752 1102 46-47 0.2827399154607946 1102 48-49 0.33319752075045983 1102 50-51 0.2979201698285081 1102 52-53 0.3403799696353431 1102 54-55 0.3587920224065755 1102 56-57 0.34160860209514254 1102 58-59 0.3499389248866507 1102 60-61 0.3662185917866907 1102 62-63 0.3862317730668252 1102 64-65 0.38674232237254813 1102 66-67 0.3997817918409936 1102 68-69 0.3788712339091731 1102 70-71 0.3579324576852656 1102 72-73 0.43818268182434394 1102 74-75 0.36425352477583317 1102 76-77 0.4184824406446239 1102 78-79 0.414610031351053 1102 80-81 0.44429995523585575 1102 82-83 0.4265300005449717 1102 84-85 0.40887290955484445 1102 86-87 0.3548945511875061 1102 88-89 0.4272806506465159 1102 90-91 0.40556450740585603 1102 92-93 0.3688786182044552 1102 94-95 0.4590212076951872 1102 96-97 0.39558607274315705 1102 98-99 0.40803545157387333 1102 100-101 0.4971605962521366 2305 1 0.08915230217051828 2305 2 0.08345095401485736 2305 3 0.10825977153030664 2305 4 0.10019842335395168 2305 5 0.14286551356482136 2305 6 0.18002934984627217 2305 7 0.11684515163621256 2305 8 0.13286669308832444 2305 9 0.11160621015720551 2305 10-11 0.10162108709047146 2305 12-13 0.14402711504389742 2305 14-15 0.2656555434238399 2305 16-17 0.26510036604803844 2305 18-19 0.2627544409245317 2305 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-1.0308407540873716 1213 62-63 -0.9750381888563453 1213 64-65 -0.9269653384594605 1213 66-67 -1.0689877438489361 1213 68-69 -1.1612488573047486 1213 70-71 -1.0881339423488328 1213 72-73 -0.9434621167094122 1213 74-75 -1.1174397577883752 1213 76-77 -1.0012791528993645 1213 78-79 -1.103585687846497 1213 80-81 -1.0853436782603936 1213 82-83 -1.1036082411008508 1213 84-85 -1.061850195128038 1213 86-87 -1.106938926591532 1213 88-89 -1.1909564486942443 1213 90-91 -1.3282208699526379 1213 92-93 -1.1422442932526948 1213 94-95 -1.1461180995337372 1213 96-97 -1.2138155668340218 1213 98-99 -1.3125970785466095 1213 100-101 -1.3573375851527274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8042.0 3 633.0 4 415.0 5 340.0 6 321.0 7 770.0 8 1482.0 9 3058.0 10 7711.0 11 13397.0 12 11379.0 13 8525.0 14 7465.0 15 6825.0 16 6454.0 17 6931.0 18 7582.0 19 8546.0 20 9528.0 21 10493.0 22 12112.0 23 13625.0 24 15637.0 25 17522.0 26 20178.0 27 24424.0 28 29336.0 29 36551.0 30 46988.0 31 60654.0 32 81210.0 33 112016.0 34 158586.0 35 240476.0 36 487499.0 37 2200350.0 38 2231165.0 39 788.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.635679900568594 14.352087134027286 19.888671945852465 38.12356101955166 2 25.77758395499937 24.543347922019063 30.261189564891964 19.4178785580896 3 23.45137618348797 27.406867645628047 29.680059501108698 19.46169666977529 4 25.174006311971237 31.39364196266002 21.279539379653325 22.152812345715418 5 26.82948496102746 34.11216244555313 21.409609433766185 17.648743159653215 6 19.131746113266853 37.68216241063815 22.142840314054038 21.04325116204096 7 18.150724287873707 16.47722284051836 42.584265777766625 22.78778709384131 8 21.859636165068167 22.111618099244748 27.603817556809062 28.424928178878023 9 24.378345692026045 23.26410532823785 26.282117244864523 26.075431734871586 10-11 24.008999649518376 31.799936682816842 21.60875132955916 22.582312338105623 12-13 22.80768899343841 25.54533831874463 27.668981862571197 23.977990825245765 14-15 22.805293233888438 26.915962235175318 27.523471606141104 22.755272924795143 16-17 23.880063994041826 27.307284252637924 26.505349271418726 22.30730248190152 18-19 23.73493497899379 27.508177748555152 26.649821456940526 22.107065815510534 20-21 23.76719710608045 27.850778647837 26.39252324401957 21.989501002062976 22-23 24.134068132262453 27.570289821402348 26.37857045227849 21.91707159405672 24-25 23.304524320639732 28.365313202632997 26.338304965188403 21.991857511538864 26-27 23.573878605489753 28.48378973349081 26.144053525799617 21.798278135219814 28-29 23.782468344524574 27.750965226413825 26.450408583829844 22.01615784523176 30-31 23.637531813451023 27.652996493635275 26.534396829510072 22.17507486340363 32-33 23.522709501385663 28.02093986104795 26.406415883915322 22.049934753651062 34-35 23.713690603542368 27.89663954244964 26.33845787653966 22.05121197746833 36-37 23.177064900437248 27.814578462457103 26.761413818794377 22.246942818311275 38-39 23.525237151277107 27.70748326134344 26.473800722992983 22.293478864386472 40-41 24.241118862617437 27.539365488281298 26.250448370982365 21.969067278118903 42-43 24.001601774805824 27.546389109355722 26.48134282516889 21.97066629066956 44-45 24.225006190557615 27.67405173415724 26.23209474990698 21.868847325378166 46-47 24.140624271458645 27.53423879455706 26.22211408607957 22.103022847904725 48-49 23.656385352564293 27.54949306673445 26.313901803184187 22.480219777517075 50-51 24.379363175340664 27.766467156751812 26.063198140910938 21.790971526996582 52-53 24.223490346737638 27.798454376124415 26.276004263497722 21.702051013640226 54-55 24.052868782402182 27.838088442841862 26.057910837808855 22.0511319369471 56-57 24.327581990073583 27.709506741764805 26.353458882209242 21.60945238595237 58-59 24.54421522158169 28.12045747232083 25.8624138450692 21.47291346102828 60-61 24.420273610599647 28.42841505786352 25.684367633043294 21.466943698493544 62-63 24.779048067950765 28.46330199231521 25.384275559607467 21.37337438012656 64-65 25.012275886141822 28.383289740834496 25.433016891093796 21.17141748192989 66-67 24.904636083700364 28.462995850617485 25.425709896528637 21.20665816915352 68-69 25.367248579298472 28.39483722833558 25.162268093518712 21.07564609884724 70-71 25.672480686188905 28.27563473710959 25.10992085630538 20.941963720396124 72-73 25.678821021479838 28.382890319544313 25.125651398618825 20.81263726035702 74-75 26.07447759932225 28.435654297261713 24.891243636216863 20.598624467199176 76-77 26.400399593146457 28.21375112044181 24.84699735165215 20.538851934759585 78-79 26.389856587614258 28.32008763901788 24.802196538536947 20.487859234830914 80-81 26.91048375083606 28.346033647049268 24.416590863828485 20.326891738286182 82-83 27.17630282875548 28.218570918575086 24.499093267191054 20.106032985478375 84-85 27.16981637470691 28.299267853617515 24.518295861121985 20.012619910553585 86-87 27.589638065959228 28.321788534521797 24.174348484569816 19.91422491494916 88-89 27.940929795711973 28.258870900959703 24.199614564959077 19.600584738369246 90-91 27.93947472709587 28.315697789005 24.232937531391126 19.511889952508003 92-93 28.377776724919222 28.32222760611522 23.94950166405802 19.350494004907546 94-95 28.704596878778275 28.25069067340466 23.92128416521786 19.1234282825992 96-97 28.645593761105875 28.395372819625216 23.87679216447895 19.08224125478996 98-99 29.01983417213402 28.44677164493497 23.72834298833391 18.805051194597098 100-101 29.204478498447635 28.492269028583983 23.5538923698526 18.74936010311578 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6902.0 1 3763.5 2 517.0 3 353.0 4 251.0 5 208.0 6 215.0 7 220.5 8 224.0 9 211.5 10 203.0 11 223.0 12 242.0 13 241.5 14 245.0 15 282.0 16 338.5 17 394.0 18 416.5 19 462.0 20 586.0 21 730.0 22 888.5 23 1150.5 24 1605.0 25 2260.0 26 3366.0 27 4920.5 28 7001.5 29 9994.5 30 13245.0 31 17278.5 32 22384.0 33 28148.5 34 35997.0 35 46694.0 36 61723.0 37 81455.0 38 105721.0 39 135811.0 40 172312.5 41 213825.0 42 258915.0 43 305027.5 44 347952.0 45 384448.5 46 409722.5 47 421830.5 48 419918.0 49 404122.5 50 374747.5 51 332671.5 52 284567.5 53 238190.0 54 190724.5 55 146584.0 56 111671.0 57 83326.5 58 61655.0 59 44822.0 60 31408.5 61 21048.0 62 14734.5 63 10777.0 64 7649.0 65 5689.0 66 4251.0 67 3092.0 68 2520.5 69 2035.0 70 1593.0 71 1146.5 72 664.5 73 417.0 74 404.5 75 355.5 76 283.5 77 142.0 78 62.0 79 43.0 80 36.0 81 32.5 82 26.5 83 30.0 84 25.0 85 14.5 86 19.0 87 15.5 88 9.0 89 6.0 90 4.5 91 4.5 92 3.0 93 5.0 94 5.5 95 2.5 96 1.5 97 1.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1670667898231414 2 0.15244506105417927 3 0.1572512774550881 4 0.14887424534787022 5 0.17554536171347707 6 0.15679434843105805 7 0.15718358426634294 8 0.14538804612749268 9 0.1725668614086885 10-11 0.14489727050909001 12-13 0.13419328503875605 14-15 0.19308635924707573 16-17 0.20142954475992103 18-19 0.1861054991577275 20-21 0.21877592437587728 22-23 0.20429804363299867 24-25 0.1955317756904959 26-27 0.18107081824480362 28-29 0.19788411399939143 30-31 0.14555727909935567 32-33 0.17289686570382132 34-35 0.17722076813492066 36-37 0.18020773008830238 38-39 0.1796661845783408 40-41 0.214401252053219 42-43 0.19978798493285005 44-45 0.18477702032860305 46-47 0.18783167547073 48-49 0.22703449340279105 50-51 0.20557575257056424 52-53 0.17095068652739695 54-55 0.15632895775843483 56-57 0.15232659797387518 58-59 0.16759141203591665 60-61 0.1619813390186586 62-63 0.15093042595600553 64-65 0.17353995099690067 66-67 0.17643383481575775 68-69 0.18844937581802987 70-71 0.16918220197142875 72-73 0.17857463190982453 74-75 0.21090659118424832 76-77 0.23723077995753605 78-79 0.22017209639374694 80-81 0.1960987061462369 82-83 0.16605139199196345 84-85 0.1759769057917277 86-87 0.1649767626206335 88-89 0.16864911811006034 90-91 0.18025849997986126 92-93 0.16467214327128013 94-95 0.15801282582847156 96-97 0.16631370309835108 98-99 0.15605818500345406 100-101 0.1629036587153119 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5909014.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.91128836916665 #Duplication Level Percentage of deduplicated Percentage of total 1 75.62232185738445 42.28141444514155 2 12.479122859230687 13.954476735534124 3 4.681023528233694 7.8516616904978385 4 2.401407947896617 5.370632490674258 5 1.2282262804590938 3.433585687466867 6 0.7978542624997982 2.6765435848317 7 0.585670200415871 2.2921902825275566 8 0.38739594786658443 1.7327845243372202 9 0.25534174624324 1.284883740620313 >10 1.46536426137108 14.178077322198067 >50 0.07201463222457646 2.6627333225272807 >100 0.023981031416870528 2.082574238939522 >500 2.1315306169541253E-4 0.07434309109367045 >1k 6.229169573350052E-5 0.12409884360998895 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.2000286342188392E-4 0.0 0.0 2 2.369261606081827E-4 0.0 0.0 3 2.5384945779448147E-4 0.0 0.0 4 2.5384945779448147E-4 0.0 0.0 5 2.5384945779448147E-4 0.0 0.0 6 2.8769605216707897E-4 0.0 0.0 7 3.046193493533777E-4 0.0 0.0 8 3.384659437259753E-4 0.0 0.0 9 3.55389240912274E-4 0.0 0.0 10-11 3.892358352848716E-4 8.461648593149382E-6 0.0 12-13 4.992372669958135E-4 2.5384945779448146E-5 0.0 14-15 5.584688071478592E-4 3.384659437259753E-5 0.0 16-17 6.600085902656518E-4 3.384659437259753E-5 0.0 18-19 9.646279396190295E-4 3.384659437259753E-5 0.0 20-21 0.0024877246863859183 3.384659437259753E-5 0.0 22-23 0.011203222737329781 6.769318874519506E-5 0.0 24-25 0.0225841400951157 6.769318874519506E-5 0.0 26-27 0.04378903146954805 8.461648593149382E-5 0.0 28-29 0.09074271951293397 8.461648593149382E-5 0.0 30-31 0.16361443719713642 1.0153978311779258E-4 0.0 32-33 0.2634195823533334 1.0153978311779258E-4 0.0 34-35 0.37115329224131133 1.0153978311779258E-4 0.0 36-37 0.5020888425717048 1.1846308030409135E-4 0.0 38-39 0.6691894789892188 1.353863774903901E-4 0.0 40-41 0.8841407382009926 1.353863774903901E-4 0.0 42-43 1.159973559040476 1.5230967467668886E-4 0.0 44-45 1.4726314745573457 1.5230967467668886E-4 0.0 46-47 1.8264722337770736 1.5230967467668886E-4 0.0 48-49 2.2315313519311344 1.6923297186298764E-4 0.0 50-51 2.707414468809856 1.77694620456137E-4 0.0 52-53 3.2649524946124684 1.946179176424358E-4 0.0 54-55 3.8946768445632385 2.0307956623558517E-4 0.0 56-57 4.57612725236393 2.0307956623558517E-4 0.0 58-59 5.325338542098564 2.0307956623558517E-4 0.0 60-61 6.148462332294356 2.0307956623558517E-4 0.0 62-63 7.052335635014573 2.1154121482873454E-4 0.0 64-65 8.024198284180745 2.2000286342188392E-4 0.0 66-67 9.056045560223753 2.284645120150333E-4 0.0 68-69 10.150153646615156 2.369261606081827E-4 0.0 70-71 11.314603417761408 2.369261606081827E-4 0.0 72-73 12.56374582967649 2.5384945779448147E-4 0.0 74-75 13.891395417238815 2.5384945779448147E-4 0.0 76-77 15.274528034626421 2.5384945779448147E-4 0.0 78-79 16.713659842403487 2.6231110638763085E-4 0.0 80-81 18.224851049599813 2.707727549807802E-4 0.0 82-83 19.806096245498825 2.707727549807802E-4 0.0 84-85 21.44781684389308 2.707727549807802E-4 0.0 86-87 23.139638863607363 2.707727549807802E-4 0.0 88-89 24.880682293188002 2.707727549807802E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCCC 410 0.0 34.7687 44-45 TCGGGGG 2200 0.0 33.362125 38-39 GGGCGCC 1565 0.0 31.880022 42-43 GGGGCGC 1415 0.0 30.726162 42-43 GGGGGCG 1565 0.0 29.4447 40-41 CTCGGGG 1645 0.0 28.734837 36-37 CGGGTAG 1035 0.0 27.314695 14-15 GATCGGG 2220 0.0 25.239773 1 GAGCGGC 1560 0.0 24.969164 9 GAGGGGC 1070 0.0 24.861023 9 GTAGGGG 1555 0.0 24.749952 18-19 AGGGTAG 2130 0.0 23.079468 28-29 GGGCCCG 395 0.0 22.856386 44-45 GGGTAGA 2220 0.0 22.785664 28-29 GGGCGGG 1235 0.0 22.694952 12-13 GGGGGGC 2035 0.0 22.644203 40-41 CGGGGGT 835 0.0 21.903934 38-39 GGGGGTC 850 0.0 21.517487 40-41 AAGGGGC 1380 0.0 20.997398 9 ATCGGGA 2675 0.0 20.769138 2 >>END_MODULE