FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n02 tsrexp_lib#29.3420000000d0db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n02 tsrexp_lib#29.3420000000d0db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5909014
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC4100.034.768744-45
TCGGGGG22000.033.36212538-39
GGGCGCC15650.031.88002242-43
GGGGCGC14150.030.72616242-43
GGGGGCG15650.029.444740-41
CTCGGGG16450.028.73483736-37
CGGGTAG10350.027.31469514-15
GATCGGG22200.025.2397731
GAGCGGC15600.024.9691649
GAGGGGC10700.024.8610239
GTAGGGG15550.024.74995218-19
AGGGTAG21300.023.07946828-29
GGGCCCG3950.022.85638644-45
GGGTAGA22200.022.78566428-29
GGGCGGG12350.022.69495212-13
GGGGGGC20350.022.64420340-41
CGGGGGT8350.021.90393438-39
GGGGGTC8500.021.51748740-41
AAGGGGC13800.020.9973989
ATCGGGA26750.020.7691382
Produced by FastQC (version 0.11.2)