##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l02n02 tsrexp_lib#24.3420000000d0ce.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8652119 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.59179837910227 33.0 33.0 33.0 33.0 33.0 2 32.540637963948484 33.0 33.0 33.0 33.0 33.0 3 32.530059861636204 33.0 33.0 33.0 33.0 33.0 4 36.4161629076068 37.0 37.0 37.0 37.0 37.0 5 36.40246741867512 37.0 37.0 37.0 37.0 37.0 6 36.42903940641593 37.0 37.0 37.0 37.0 37.0 7 36.44255031628668 37.0 37.0 37.0 37.0 37.0 8 36.4579741679466 37.0 37.0 37.0 37.0 37.0 9 36.41926630921281 37.0 37.0 37.0 37.0 37.0 10-11 36.402539193 37.0 37.0 37.0 37.0 37.0 12-13 36.40415001226867 37.0 37.0 37.0 37.0 37.0 14-15 38.75978809352946 40.0 40.0 40.0 37.0 40.0 16-17 38.73550132632249 40.0 40.0 40.0 37.0 40.0 18-19 38.73952785439035 40.0 40.0 40.0 37.0 40.0 20-21 38.68496312868559 40.0 40.0 40.0 37.0 40.0 22-23 38.71303307316971 40.0 40.0 40.0 37.0 40.0 24-25 38.68114978538783 40.0 40.0 40.0 37.0 40.0 26-27 38.66220893401952 40.0 40.0 40.0 37.0 40.0 28-29 38.61291684730642 40.0 40.0 40.0 37.0 40.0 30-31 38.59491420541026 40.0 40.0 40.0 37.0 40.0 32-33 38.514234547629314 40.0 40.0 40.0 37.0 40.0 34-35 38.50099698120194 40.0 40.0 40.0 37.0 40.0 36-37 38.46837219876426 40.0 40.0 40.0 37.0 40.0 38-39 38.44724448427027 40.0 40.0 40.0 37.0 40.0 40-41 38.40235449836046 40.0 38.5 40.0 37.0 40.0 42-43 38.34562174884557 40.0 37.0 40.0 37.0 40.0 44-45 38.30192580568991 40.0 37.0 40.0 37.0 40.0 46-47 38.2113471855854 40.0 37.0 40.0 37.0 40.0 48-49 38.11579435049379 40.0 37.0 40.0 37.0 40.0 50-51 36.74507793986652 38.5 37.0 38.5 35.0 40.0 52-53 37.06758003444011 38.5 37.0 40.0 33.0 40.0 54-55 37.741961824611984 40.0 37.0 40.0 33.0 40.0 56-57 37.70100214756639 40.0 37.0 40.0 33.0 40.0 58-59 37.5017929711785 40.0 37.0 40.0 33.0 40.0 60-61 37.31502161493618 37.0 37.0 40.0 33.0 40.0 62-63 37.08690397115435 37.0 37.0 40.0 33.0 40.0 64-65 36.80616800346829 37.0 37.0 40.0 33.0 40.0 66-67 36.55045203377346 37.0 37.0 40.0 33.0 40.0 68-69 36.270961772486025 37.0 37.0 37.0 33.0 40.0 70-71 36.01078261868567 37.0 37.0 37.0 33.0 40.0 72-73 35.71757641105029 37.0 37.0 37.0 33.0 38.5 74-75 35.56048448940658 37.0 37.0 37.0 33.0 37.0 76-77 35.3463236000337 37.0 37.0 37.0 33.0 37.0 78-79 35.24717025967858 37.0 37.0 37.0 33.0 37.0 80-81 35.14626827254688 37.0 37.0 37.0 33.0 37.0 82-83 35.07136506097524 37.0 37.0 37.0 33.0 37.0 84-85 35.022404858278065 37.0 35.0 37.0 33.0 37.0 86-87 34.96098972979914 37.0 33.0 37.0 33.0 37.0 88-89 34.877807216937256 37.0 33.0 37.0 33.0 37.0 90-91 34.81841194047377 37.0 33.0 37.0 33.0 37.0 92-93 34.79577505811004 37.0 33.0 37.0 33.0 37.0 94-95 34.73741773547035 37.0 33.0 37.0 33.0 37.0 96-97 34.66758778976572 37.0 33.0 37.0 33.0 37.0 98-99 34.612734348660716 37.0 33.0 37.0 33.0 37.0 100-101 32.793116171888066 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1621928177247156 2306 2 0.22275656723862625 2306 3 0.25024810597887637 2306 4 0.2815267888885131 2306 5 0.2647991804670653 2306 6 0.32407104417956845 2306 7 0.2660819992507939 2306 8 0.3092010314325009 2306 9 0.2877293152900009 2306 10-11 0.2781200655435043 2306 12-13 0.26106726817563697 2306 14-15 0.5395070910603792 2306 16-17 0.535206319710845 2306 18-19 0.5749355279582531 2306 20-21 0.5824481163532127 2306 22-23 0.5911025260507543 2306 24-25 0.5939527305610497 2306 26-27 0.6069587386864654 2306 28-29 0.6477736430463494 2306 30-31 0.617747431548068 2306 32-33 0.702624685531724 2306 34-35 0.7049725590145357 2306 36-37 0.6764424823122326 2306 38-39 0.6315512336084126 2306 40-41 0.664754260993 2306 42-43 0.6452573807709499 2306 44-45 0.69123743574211 2306 46-47 0.7169281002262196 2306 48-49 0.6995816829018437 2306 50-51 0.6156611940969796 2306 52-53 0.7142908117308551 2306 54-55 0.7150683451177784 2306 56-57 0.6865528606436726 2306 58-59 0.7211264503275103 2306 60-61 0.7248839512813205 2306 62-63 0.7002378468426471 2306 64-65 0.7543735968885841 2306 66-67 0.685341752260463 2306 68-69 0.7240492873431634 2306 70-71 0.733160532826723 2306 72-73 0.7934027369511512 2306 74-75 0.7531736306419035 2306 76-77 0.8152895912462839 2306 78-79 0.8280184049573123 2306 80-81 0.8025802329066494 2306 82-83 0.826651889239578 2306 84-85 0.8255303486622481 2306 86-87 0.7913552409573796 2306 88-89 0.8794526411851677 2306 90-91 0.8649741230891763 2306 92-93 0.8752055031281429 2306 94-95 0.8941503910085089 2306 96-97 0.9342459943952477 2306 98-99 0.8394857587696833 2306 100-101 0.906075170385428 2307 1 0.12969793780168004 2307 2 0.18268138823379587 2307 3 0.20827193930995946 2307 4 0.22381517467588452 2307 5 0.23527119805699215 2307 6 0.24371436132328483 2307 7 0.23470894956604837 2307 8 0.2509846058356757 2307 9 0.23643088357273712 2307 10-11 0.28157777906450576 2307 12-13 0.2615908724775835 2307 14-15 0.4471873867991789 2307 16-17 0.45857766281530843 2307 18-19 0.44014483797582216 2307 20-21 0.5500586295575474 2307 22-23 0.4931700648025057 2307 24-25 0.46675153749495024 2307 26-27 0.5002465929551221 2307 28-29 0.5241591776455792 2307 30-31 0.5017662520898796 2307 32-33 0.6278928299104649 2307 34-35 0.5878187626037459 2307 36-37 0.5936366697452797 2307 38-39 0.5717706872179278 2307 40-41 0.5618192778268707 2307 42-43 0.5292365070035601 2307 44-45 0.5066582256716998 2307 46-47 0.5411838838765277 2307 48-49 0.553900451245184 2307 50-51 0.47941907971221553 2307 52-53 0.5882928848799764 2307 54-55 0.5468980413136038 2307 56-57 0.5108603171784125 2307 58-59 0.5690661384457414 2307 60-61 0.5560879197099453 2307 62-63 0.5910066250774761 2307 64-65 0.6506383104444851 2307 66-67 0.5932717646234593 2307 68-69 0.6215154642164862 2307 70-71 0.6205470136722866 2307 72-73 0.7095659418605109 2307 74-75 0.7036934671930126 2307 76-77 0.695406299238023 2307 78-79 0.6959399149765133 2307 80-81 0.6685322145877421 2307 82-83 0.7168044431176526 2307 84-85 0.6844345583797207 2307 86-87 0.7196282353756729 2307 88-89 0.7290810028332615 2307 90-91 0.7949231707527176 2307 92-93 0.758514348627557 2307 94-95 0.6607955227230278 2307 96-97 0.6778494199499576 2307 98-99 0.7619330403573201 2307 100-101 0.7710820058127013 2201 1 0.17640089702729256 2201 2 0.17156544124514284 2201 3 0.2072534185664594 2201 4 0.23791744292111616 2201 5 0.24738199853029386 2201 6 0.2660346579501649 2201 7 0.19835396844185027 2201 8 0.2016947686084194 2201 9 0.24730420231695405 2201 10-11 0.23933223990260188 2201 12-13 0.2565742163204874 2201 14-15 0.4683644046375548 2201 16-17 0.4755853589728716 2201 18-19 0.4717023828700775 2201 20-21 0.5504369431809337 2201 22-23 0.5153569288420314 2201 24-25 0.5222998110383372 2201 26-27 0.555059990343004 2201 28-29 0.5818942644759417 2201 30-31 0.574027442715618 2201 32-33 0.6058184500858701 2201 34-35 0.6423794330262353 2201 36-37 0.6095391774182346 2201 38-39 0.5851411607122543 2201 40-41 0.6096075647192123 2201 42-43 0.5953283889991283 2201 44-45 0.6179906426281008 2201 46-47 0.6455402856577095 2201 48-49 0.6402619421602651 2201 50-51 0.5722585634946071 2201 52-53 0.6774088925645358 2201 54-55 0.6974276337768757 2201 56-57 0.6878132648034381 2201 58-59 0.674684776769432 2201 60-61 0.718326124611032 2201 62-63 0.6946312838960793 2201 64-65 0.6756265855705124 2201 66-67 0.6845790652840194 2201 68-69 0.6512812888604316 2201 70-71 0.6792593459610075 2201 72-73 0.777468731676187 2201 74-75 0.6824686228479706 2201 76-77 0.7626458620286556 2201 78-79 0.7750897021058236 2201 80-81 0.779301925633817 2201 82-83 0.781084951198288 2201 84-85 0.7538971085936694 2201 86-87 0.8240534379301181 2201 88-89 0.8447699821146273 2201 90-91 0.7756314287513177 2201 92-93 0.7950909319412887 2201 94-95 0.7940088124740825 2201 96-97 0.8301533058963528 2201 98-99 0.8456466984268545 2201 100-101 0.8250891886445046 1101 1 0.14855849209059357 1101 2 0.16219121834777894 1101 3 0.17523763459828956 1101 4 0.20782566372098188 1101 5 0.21824335260798478 1101 6 0.28499714484078 1101 7 0.21797614789714714 1101 8 0.23884770781331355 1101 9 0.2618726806013285 1101 10-11 0.28609371534345485 1101 12-13 0.26610614037255687 1101 14-15 0.4914076939846268 1101 16-17 0.4747217614336492 1101 18-19 0.47477844229747745 1101 20-21 0.5241426618454526 1101 22-23 0.5096554450490132 1101 24-25 0.5507996279124114 1101 26-27 0.5048754622537857 1101 28-29 0.5386428433917203 1101 30-31 0.5237081042124174 1101 32-33 0.5671649425824299 1101 34-35 0.5500070163856989 1101 36-37 0.5732071795751992 1101 38-39 0.5617908313558857 1101 40-41 0.5442407317300493 1101 42-43 0.5753820225959103 1101 44-45 0.5823445223299117 1101 46-47 0.5268180924172938 1101 48-49 0.6180338503729601 1101 50-51 0.6025744354443034 1101 52-53 0.6319008329965712 1101 54-55 0.6695919017867382 1101 56-57 0.6454001854227442 1101 58-59 0.7057834106905645 1101 60-61 0.7253272280967806 1101 62-63 0.6457717158029652 1101 64-65 0.696713096690516 1101 66-67 0.6031696240951518 1101 68-69 0.66025032030673 1101 70-71 0.6356048451176903 1101 72-73 0.7324407863896241 1101 74-75 0.6813451182637422 1101 76-77 0.7200393516654913 1101 78-79 0.6516663757821846 1101 80-81 0.6792350948070336 1101 82-83 0.6992858606110914 1101 84-85 0.7104419514702727 1101 86-87 0.7321954294482182 1101 88-89 0.7988239576365643 1101 90-91 0.8336026941987669 1101 92-93 0.7456819016031915 1101 94-95 0.7127004790956946 1101 96-97 0.7451783150864273 1101 98-99 0.7117577423392305 1101 100-101 0.820893679081415 2304 1 0.12338131483298298 2304 2 0.17037341156392927 2304 3 0.15931460826545418 2304 4 0.20682598018431975 2304 5 0.2271129146861881 2304 6 0.1999311722778927 2304 7 0.16434172507010203 2304 8 0.21262215475213253 2304 9 0.21495320523757755 2304 10-11 0.2205993410834779 2304 12-13 0.23378539269224774 2304 14-15 0.40067838863476624 2304 16-17 0.42479595709416884 2304 18-19 0.43324845120910993 2304 20-21 0.4692289934530365 2304 22-23 0.4511714835102296 2304 24-25 0.38491657436430415 2304 26-27 0.43300702279071146 2304 28-29 0.4132553431988484 2304 30-31 0.44157938893421544 2304 32-33 0.5110683941872693 2304 34-35 0.5185882401817636 2304 36-37 0.5202960807370332 2304 38-39 0.49234739779556946 2304 40-41 0.5142276626964986 2304 42-43 0.47562241246011894 2304 44-45 0.5025216297152113 2304 46-47 0.5032803306545048 2304 48-49 0.4483753629396432 2304 50-51 0.473961236331796 2304 52-53 0.5103150779811259 2304 54-55 0.531463949863543 2304 56-57 0.5226173575913151 2304 58-59 0.5301137937626024 2304 60-61 0.5788234784283048 2304 62-63 0.5312284347098597 2304 64-65 0.5461032205886838 2304 66-67 0.5507782733556539 2304 68-69 0.47533252071391274 2304 70-71 0.46602139763531625 2304 72-73 0.552875315015001 2304 74-75 0.5580212531138073 2304 76-77 0.5990804095089857 2304 78-79 0.577895953779624 2304 80-81 0.6457988647908763 2304 82-83 0.631128477888133 2304 84-85 0.6101272203782315 2304 86-87 0.5806412217812991 2304 88-89 0.6204291750020445 2304 90-91 0.6329727225290824 2304 92-93 0.5875229409305334 2304 94-95 0.5945189977037089 2304 96-97 0.6583318359085268 2304 98-99 0.6627158955208898 2304 100-101 0.7415508674720073 2202 1 0.15114926106310378 2202 2 0.19815426499570066 2202 3 0.20408171869099334 2202 4 0.21776439068774778 2202 5 0.25115763940531366 2202 6 0.2524364031744426 2202 7 0.2111896788673775 2202 8 0.2684294780705727 2202 9 0.2622056479571242 2202 10-11 0.28575492664336366 2202 12-13 0.25404005051537126 2202 14-15 0.4146679920103793 2202 16-17 0.4181351134313829 2202 18-19 0.4606266189306041 2202 20-21 0.4898904812105016 2202 22-23 0.46744186534194654 2202 24-25 0.4764862419342677 2202 26-27 0.4897195585306875 2202 28-29 0.511801606342523 2202 30-31 0.4841629678101924 2202 32-33 0.5064670717052664 2202 34-35 0.5531474624089725 2202 36-37 0.5457970415010394 2202 38-39 0.5738112642743616 2202 40-41 0.5676982447163894 2202 42-43 0.562782467454916 2202 44-45 0.5674622217888015 2202 46-47 0.5818011969808126 2202 48-49 0.5686565311426222 2202 50-51 0.5510923956452629 2202 52-53 0.5986807225534889 2202 54-55 0.5987860788178736 2202 56-57 0.6168525837489085 2202 58-59 0.6258577072894056 2202 60-61 0.627646898419151 2202 62-63 0.5988539145382461 2202 64-65 0.6457724419111273 2202 66-67 0.5940558357673567 2202 68-69 0.5891037456263319 2202 70-71 0.6185259009200124 2202 72-73 0.7079772977256766 2202 74-75 0.6132968187054857 2202 76-77 0.6835969240836874 2202 78-79 0.622540667236585 2202 80-81 0.684526290632995 2202 82-83 0.7155873845848788 2202 84-85 0.6761798880880221 2202 86-87 0.6885031944445998 2202 88-89 0.712185364289958 2202 90-91 0.6987214699294526 2202 92-93 0.6884841478298114 2202 94-95 0.692086591263525 2202 96-97 0.7740821018032094 2202 98-99 0.7902241159531087 2202 100-101 0.7523223385070494 1102 1 0.06332128598266706 1102 2 0.10440975194008217 1102 3 0.12312847966045126 1102 4 0.08167471601512943 1102 5 0.10246256986577151 1102 6 0.11587226794359395 1102 7 0.1148799411454462 1102 8 0.13219902191379163 1102 9 0.10293355890026845 1102 10-11 0.11482162821599928 1102 12-13 0.1344158596081968 1102 14-15 0.1458952655876331 1102 16-17 0.17198095904191035 1102 18-19 0.24006665735954869 1102 20-21 0.2622516540802948 1102 22-23 0.1802598045506727 1102 24-25 0.18534307782609005 1102 26-27 0.25542859377803495 1102 28-29 0.22812583506179607 1102 30-31 0.25897957181691567 1102 32-33 0.3123722441923391 1102 34-35 0.16397587049507933 1102 36-37 0.1918100379852703 1102 38-39 0.24045485592403537 1102 40-41 0.25339523934619024 1102 42-43 0.24005583828626698 1102 44-45 0.24592938172088452 1102 46-47 0.2687767118823601 1102 48-49 0.297431700659601 1102 50-51 0.267006641094639 1102 52-53 0.29191140869140497 1102 54-55 0.3421613106507593 1102 56-57 0.3059258569050485 1102 58-59 0.32772031165215054 1102 60-61 0.3079044748648201 1102 62-63 0.3308041351574289 1102 64-65 0.3498830086822764 1102 66-67 0.2802540411373542 1102 68-69 0.33068953235489573 1102 70-71 0.3072475117885247 1102 72-73 0.4377130088421666 1102 74-75 0.3685696280622963 1102 76-77 0.426344601262052 1102 78-79 0.34161381410953595 1102 80-81 0.35823166318179744 1102 82-83 0.3572554132482466 1102 84-85 0.3015489811002112 1102 86-87 0.2939820882717612 1102 88-89 0.3784066479149857 1102 90-91 0.404532792141282 1102 92-93 0.33200464177198796 1102 94-95 0.4202403745746679 1102 96-97 0.3559282116370497 1102 98-99 0.3744145884198531 1102 100-101 0.44326051098027364 2305 1 0.07520813203005616 2305 2 0.05558534475507315 2305 3 0.09010942038100467 2305 4 0.10096156240058463 2305 5 0.11781497726198609 2305 6 0.1662020699682003 2305 7 0.07027975909189621 2305 8 0.13478496228944437 2305 9 0.1277119341773698 2305 10-11 0.07975237373486976 2305 12-13 0.11933256234134149 2305 14-15 0.2488561953888393 2305 16-17 0.25733888487098966 2305 18-19 0.25548589204402816 2305 20-21 0.27511864375021844 2305 22-23 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-1.0800323002282965 1213 80-81 -1.1025899203460767 1213 82-83 -1.1089547190808986 1213 84-85 -1.0738755936901967 1213 86-87 -1.156509694082338 1213 88-89 -1.2101592352318562 1213 90-91 -1.3521037528559816 1213 92-93 -1.230978008516189 1213 94-95 -1.1558262501588459 1213 96-97 -1.2206487772396528 1213 98-99 -1.2999232678201196 1213 100-101 -1.4094778391994396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11294.0 3 950.0 4 600.0 5 466.0 6 429.0 7 581.0 8 988.0 9 2097.0 10 5853.0 11 10372.0 12 9533.0 13 8548.0 14 7996.0 15 7874.0 16 8508.0 17 9211.0 18 10401.0 19 11507.0 20 13041.0 21 14954.0 22 16666.0 23 19244.0 24 21832.0 25 25221.0 26 29559.0 27 34575.0 28 42548.0 29 52681.0 30 66772.0 31 86373.0 32 116189.0 33 160360.0 34 227928.0 35 344617.0 36 702365.0 37 3163353.0 38 3405402.0 39 1231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.967401538323156 15.656786839987047 18.706969015317824 38.668842606371975 2 24.96339452495527 26.90865287453391 29.356884919794368 18.771067680716456 3 22.414431955049753 28.3233773802985 29.72391691751887 19.538273747132877 4 24.87341935512247 31.17632236769841 22.15866456305617 21.791593714122946 5 26.91880792464683 33.57223990950062 22.015137181795417 17.49381498405714 6 19.457082817361293 37.897420012468984 22.4283872479261 20.21710992224362 7 18.437060997552468 17.336296363520187 42.222867623022545 22.003775015904793 8 22.125558519149518 22.508334746329155 28.229387959655256 27.136718774866075 9 24.19049665468843 23.430436957626362 27.405019744464067 24.97404664322114 10-11 24.103525124780013 31.766249844981637 22.120748409842914 22.009476620395436 12-13 22.990411124172226 26.025602932067198 27.651565891804797 23.332420051955776 14-15 23.014658252408747 27.358659017593933 27.559531708519103 22.06715102147822 16-17 23.799075813966738 27.596230390114346 26.73171969429736 21.872974101621555 18-19 23.666282384611318 27.84102938573788 26.7784923009178 21.714195928732998 20-21 23.764146856092363 27.96627254104781 26.667326499526638 21.602254103333184 22-23 23.95559822766745 27.76522494261761 26.665597740686547 21.61357908902839 24-25 23.33974939159 28.251623739060435 26.694411428751412 21.714215440598156 26-27 23.682433729112994 28.386698334597 26.48602623725117 21.44484169903884 28-29 23.770344108175813 27.796741653913 26.777133352108713 21.655780885802482 30-31 23.514145881096947 27.825934027574228 26.84361790527846 21.81630218605036 32-33 23.67620609991948 28.007636331851643 26.659249530109737 21.656908038119145 34-35 23.77335131898996 27.861063002322613 26.649844574426613 21.71574110426081 36-37 23.321676176097366 27.86317997832591 26.989512610801846 21.825631234774875 38-39 23.658485994768206 27.85153116453743 26.717735298158058 21.772247542536306 40-41 23.997495944083806 27.580803434795666 26.727519163353453 21.694181457767076 42-43 23.763148398799714 27.570631243578664 26.945879643397454 21.72034071422417 44-45 24.042024113371134 27.717779343069004 26.630374089614843 21.609822453945018 46-47 23.987591425295548 27.56279145919901 26.698073685841344 21.751543429664093 48-49 23.596929312006196 27.597884507421906 26.884916733451767 21.920269447120127 50-51 24.09151275615601 27.72392723474124 26.654492992126873 21.530067016975877 52-53 24.082209520660566 27.55712895311216 26.77560897281166 21.585052553415615 54-55 23.853441879470264 27.664720460514662 26.704300135547626 21.777537524467448 56-57 24.087456608525855 27.701059220753365 26.70888039289902 21.502603777821765 58-59 24.27405753708412 27.847089263916125 26.503396551914754 21.375456647085002 60-61 24.100534228593627 27.95264773331529 26.473102109207115 21.47371592888397 62-63 24.379575553250646 28.03958988968033 26.191803974499074 21.389030582569955 64-65 24.58656757119877 27.929795053519808 26.209594300914613 21.274043074366812 66-67 24.355290426406402 28.008694846769245 26.345889340418882 21.290125386405474 68-69 24.792947420313567 27.992331182231688 26.033760514157006 21.180960883297743 70-71 24.981563771868203 27.91596298352698 25.95499661722709 21.147476627377724 72-73 24.874327816778536 28.03235301813537 26.02499040771995 21.068328757366142 74-75 25.271617821814402 28.07288951306404 25.73755446066246 20.917938204459098 76-77 25.55653878954634 27.85718468681249 25.723326218869197 20.862950304771964 78-79 25.429354387954707 28.022298838113297 25.7960983345889 20.752248439343102 80-81 25.952624035798866 28.06307253310041 25.352264928540137 20.632038502560597 82-83 26.17752440894492 27.962513611815364 25.36671661741533 20.493245361824382 84-85 26.104474512758948 28.060761590544487 25.426614689965298 20.40814920673127 86-87 26.52316284826725 28.122352085341955 25.067717446411663 20.28676761997913 88-89 26.853184093151434 27.997005085970645 25.09365184153351 20.056158979344413 90-91 26.816563024168044 28.05529437005241 25.077820157947855 20.050322447831693 92-93 27.24207895931641 28.15215154054225 24.80281471332048 19.802954786820866 94-95 27.579156155776385 28.014641982461225 24.76672985982232 19.63947200194007 96-97 27.561482123247405 28.118802963857526 24.702785371307595 19.616929541587474 98-99 27.990631349639074 28.206784226621885 24.47881123615288 19.32377318758617 100-101 28.234940431291434 28.12821383705461 24.380286552136567 19.256559179517385 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9631.0 1 5314.0 2 767.5 3 454.0 4 349.0 5 322.0 6 308.0 7 294.5 8 285.5 9 287.0 10 280.0 11 267.5 12 290.0 13 325.5 14 368.5 15 411.0 16 439.5 17 484.0 18 565.5 19 675.0 20 783.0 21 928.0 22 1181.0 23 1620.5 24 2296.0 25 3285.0 26 5046.5 27 7468.5 28 10916.5 29 15936.5 30 21698.0 31 28851.0 32 38203.0 33 49173.0 34 62773.5 35 81973.5 36 109368.5 37 142863.5 38 183685.0 39 232783.0 40 290839.5 41 353424.5 42 418660.0 43 482191.5 44 535924.0 45 576409.5 46 599584.5 47 603959.0 48 588655.5 49 556308.0 50 507054.5 51 444777.5 52 376248.0 53 311841.0 54 248557.0 55 190277.5 56 144944.0 57 109153.5 58 81796.0 59 60010.0 60 42579.5 61 29434.0 62 21265.5 63 15388.5 64 10632.5 65 7974.5 66 6300.0 67 4623.5 68 3662.0 69 3063.0 70 2605.0 71 1891.0 72 1053.5 73 719.0 74 674.0 75 565.0 76 405.0 77 174.0 78 73.0 79 58.5 80 45.5 81 36.0 82 30.0 83 21.0 84 14.0 85 10.5 86 13.0 87 14.0 88 9.0 89 6.0 90 8.5 91 7.5 92 3.0 93 1.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16194876653915646 2 0.14679640906464647 3 0.1529567496702253 4 0.14297075664354594 5 0.17003927014873468 6 0.15123462818761507 7 0.15273715028653675 8 0.13966520802591828 9 0.1676814662396576 10-11 0.13885037873381076 12-13 0.1284829762512513 14-15 0.18840471334247716 16-17 0.19718868869001918 18-19 0.18108280757580889 20-21 0.21520161708363006 22-23 0.19976031305163508 24-25 0.19064693862856025 26-27 0.17568528588199028 28-29 0.19295851108843973 30-31 0.14020264862284026 32-33 0.1671729202984841 34-35 0.17181918094284185 36-37 0.1742810056126135 38-39 0.17469130972424213 40-41 0.2095035909700271 42-43 0.19471530615794813 44-45 0.1786672143552348 46-47 0.18299563378635914 48-49 0.22341925717850158 50-51 0.20071961562248508 52-53 0.16569351392416123 54-55 0.15128085963681268 56-57 0.14735118645501755 58-59 0.16204700836870137 60-61 0.1570540118553617 62-63 0.14551348634941336 64-65 0.16784905524299887 66-67 0.17127596141477017 68-69 0.18315166492740104 70-71 0.1645261698319221 72-73 0.1729634093104822 74-75 0.2056201492374296 76-77 0.2331625350968936 78-79 0.21468729221130683 80-81 0.19113814777628463 82-83 0.16115705297164776 84-85 0.17091188875233917 86-87 0.15990880384331285 88-89 0.16344550970692845 90-91 0.17588754847222976 92-93 0.15946382614478602 94-95 0.15327459088345874 96-97 0.1608507696207137 98-99 0.15134442787945937 100-101 0.15823869274104993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8652119.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.95534941768164 #Duplication Level Percentage of deduplicated Percentage of total 1 72.58430459934776 37.71142907698549 2 13.75180584481118 14.289597555825631 3 5.570224223670592 8.6820883762692 4 2.5532124957599187 5.306121894191903 5 1.5075159526026223 3.916175903509921 6 0.8618389663855315 2.6866286784200324 7 0.581670090929398 2.1154610974036068 8 0.44929265536060414 1.8674525520046563 9 0.33030061828954105 1.544479563249845 >10 1.6879249673976555 15.223297366685376 >50 0.08547766284199446 3.011767982324774 >100 0.03543887905541508 3.2347019039326232 >500 8.076179791458965E-4 0.26401687194958945 >1k 1.8542556863661614E-4 0.14678117724741943 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.0804152138915335E-4 0.0 0.0 2 2.1959938368855075E-4 0.0 0.0 3 3.1206228208373E-4 0.0 0.0 4 6.472402887662548E-4 0.0 0.0 5 7.281453248620367E-4 0.0 0.0 6 8.78397534754203E-4 0.0 0.0 7 9.015132593529978E-4 0.0 0.0 8 9.593025708499849E-4 0.0 0.0 9 0.001051765469245164 0.0 0.0 10-11 0.0013985013382270864 0.0 0.0 12-13 0.0017914686564065984 1.1557862299397408E-5 0.0 14-15 0.00231735139102918 1.1557862299397408E-5 0.0 16-17 0.002773886951855378 1.1557862299397408E-5 0.0 18-19 0.003236201443831274 2.3115724598794816E-5 0.0 20-21 0.004074146460537586 2.3115724598794816E-5 5.778931149698704E-6 22-23 0.006056319844884242 2.3115724598794816E-5 1.1557862299397408E-5 24-25 0.00857015489500318 3.467358689819222E-5 1.1557862299397408E-5 26-27 0.013002595086822083 5.201038034728833E-5 1.1557862299397408E-5 28-29 0.023381555431680957 5.778931149698704E-5 1.1557862299397408E-5 30-31 0.04259072257327945 5.778931149698704E-5 1.1557862299397408E-5 32-33 0.07193613495144946 5.778931149698704E-5 1.1557862299397408E-5 34-35 0.11000773336566452 8.090503609578185E-5 1.1557862299397408E-5 36-37 0.16270002758861732 9.246289839517926E-5 1.1557862299397408E-5 38-39 0.233711533556115 9.246289839517926E-5 1.1557862299397408E-5 40-41 0.32483949885571384 9.246289839517926E-5 1.1557862299397408E-5 42-43 0.44396060664445325 1.0402076069457668E-4 1.1557862299397408E-5 44-45 0.5841805920607426 1.1557862299397407E-4 1.1557862299397408E-5 46-47 0.748163542364593 1.1557862299397407E-4 1.1557862299397408E-5 48-49 0.9451557473955223 1.1557862299397407E-4 1.1557862299397408E-5 50-51 1.1793816058239606 1.1557862299397407E-4 1.1557862299397408E-5 52-53 1.4608848999880837 1.1557862299397407E-4 1.1557862299397408E-5 54-55 1.789191757533617 1.3291541644307018E-4 1.1557862299397408E-5 56-57 2.16334287588971 1.3869434759276888E-4 1.1557862299397408E-5 58-59 2.5950983799459992 1.3869434759276888E-4 2.3115724598794816E-5 60-61 3.088151006707143 1.3869434759276888E-4 2.3115724598794816E-5 62-63 3.6495568311069233 1.4447327874246758E-4 2.3115724598794816E-5 64-65 4.27297636567412 1.502522098921663E-4 2.3115724598794816E-5 66-67 4.95464174729913 1.502522098921663E-4 2.3115724598794816E-5 68-69 5.697893198186479 1.502522098921663E-4 2.3115724598794816E-5 70-71 6.510058403033986 1.502522098921663E-4 2.3115724598794816E-5 72-73 7.395743169967958 1.502522098921663E-4 2.3115724598794816E-5 74-75 8.357316860759774 1.502522098921663E-4 2.3115724598794816E-5 76-77 9.378257511252446 1.502522098921663E-4 2.3115724598794816E-5 78-79 10.452416338702692 1.618100721915637E-4 2.3115724598794816E-5 80-81 11.597638682500785 1.618100721915637E-4 2.3115724598794816E-5 82-83 12.814173036686157 1.618100721915637E-4 3.467358689819222E-5 84-85 14.104348310512142 1.618100721915637E-4 3.467358689819222E-5 86-87 15.465876047243455 1.733679344909611E-4 3.467358689819222E-5 88-89 16.877183496898276 1.733679344909611E-4 3.467358689819222E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCCC 430 0.0 32.045174 44-45 GGGCGCC 1560 0.0 25.736912 42-43 GGGGGCG 1625 0.0 23.826729 40-41 TCGGGGG 2505 0.0 23.042185 38-39 GGGGCGC 1610 0.0 22.87179 42-43 GGGGGGC 1875 0.0 20.143087 40-41 GGGGCCC 580 0.0 19.251873 44-45 CTCGGGG 2050 0.0 18.65519 36-37 CGGGTAG 1155 0.0 17.483007 14-15 GCGCCCG 665 0.0 17.148363 44-45 CGGGGGG 2345 0.0 16.612162 38-39 GGGCCCG 420 0.0 16.40408 44-45 GTAGGGG 1550 0.0 16.400097 18-19 AGGGTAG 2165 0.0 16.124788 28-29 GAGCGGC 1915 0.0 15.379345 9 GGGTAGA 2460 0.0 15.05997 28-29 TAGGGGA 1810 0.0 14.4380455 18-19 GATCGGG 2885 0.0 13.82627 1 CGGGGGT 965 0.0 13.784335 38-39 GGGTAGG 1585 0.0 13.639869 16-17 >>END_MODULE