FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n02 tsrexp_lib#24.3420000000d0ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n02 tsrexp_lib#24.3420000000d0ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8652119
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC4300.032.04517444-45
GGGCGCC15600.025.73691242-43
GGGGGCG16250.023.82672940-41
TCGGGGG25050.023.04218538-39
GGGGCGC16100.022.8717942-43
GGGGGGC18750.020.14308740-41
GGGGCCC5800.019.25187344-45
CTCGGGG20500.018.6551936-37
CGGGTAG11550.017.48300714-15
GCGCCCG6650.017.14836344-45
CGGGGGG23450.016.61216238-39
GGGCCCG4200.016.4040844-45
GTAGGGG15500.016.40009718-19
AGGGTAG21650.016.12478828-29
GAGCGGC19150.015.3793459
GGGTAGA24600.015.0599728-29
TAGGGGA18100.014.438045518-19
GATCGGG28850.013.826271
CGGGGGT9650.013.78433538-39
GGGTAGG15850.013.63986916-17
Produced by FastQC (version 0.11.2)