##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l02n02 tsrexp_lib#21.3420000000d0b1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12486195 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56538897558463 33.0 33.0 33.0 33.0 33.0 2 32.49402584214006 33.0 33.0 33.0 33.0 33.0 3 32.47530236393072 33.0 33.0 33.0 33.0 33.0 4 36.35151861716079 37.0 37.0 37.0 37.0 37.0 5 36.340883031219676 37.0 37.0 37.0 37.0 37.0 6 36.390848933562225 37.0 37.0 37.0 37.0 37.0 7 36.373067055255824 37.0 37.0 37.0 37.0 37.0 8 36.41467925176565 37.0 37.0 37.0 37.0 37.0 9 36.345382320234464 37.0 37.0 37.0 37.0 37.0 10-11 36.3331720752399 37.0 37.0 37.0 37.0 37.0 12-13 36.33505359318832 37.0 37.0 37.0 37.0 37.0 14-15 38.6772586044027 40.0 40.0 40.0 37.0 40.0 16-17 38.650631117005624 40.0 40.0 40.0 37.0 40.0 18-19 38.65959789992067 40.0 40.0 40.0 37.0 40.0 20-21 38.60805253321769 40.0 40.0 40.0 37.0 40.0 22-23 38.671537526043764 40.0 40.0 40.0 37.0 40.0 24-25 38.61444042800869 40.0 40.0 40.0 37.0 40.0 26-27 38.60125798932341 40.0 40.0 40.0 37.0 40.0 28-29 38.53556163426889 40.0 40.0 40.0 37.0 40.0 30-31 38.51069905603749 40.0 40.0 40.0 37.0 40.0 32-33 38.43215327007147 40.0 40.0 40.0 37.0 40.0 34-35 38.4209424488405 40.0 40.0 40.0 37.0 40.0 36-37 38.390208626407 40.0 40.0 40.0 37.0 40.0 38-39 38.375875877318904 40.0 40.0 40.0 37.0 40.0 40-41 38.33853772105914 40.0 37.0 40.0 37.0 40.0 42-43 38.267998617673356 40.0 37.0 40.0 37.0 40.0 44-45 38.23541575315779 40.0 37.0 40.0 37.0 40.0 46-47 38.128540640283134 40.0 37.0 40.0 37.0 40.0 48-49 38.042302318680754 40.0 37.0 40.0 37.0 40.0 50-51 36.66340454397837 38.5 37.0 38.5 35.0 40.0 52-53 36.99124997647402 38.5 37.0 40.0 33.0 40.0 54-55 37.66079690410089 40.0 37.0 40.0 33.0 40.0 56-57 37.63768598039675 40.0 37.0 40.0 33.0 40.0 58-59 37.43119264916173 40.0 37.0 40.0 33.0 40.0 60-61 37.273312246044526 37.0 37.0 40.0 33.0 40.0 62-63 37.046642992520944 37.0 37.0 40.0 33.0 40.0 64-65 36.75857028502278 37.0 37.0 40.0 33.0 40.0 66-67 36.48833952216828 37.0 37.0 40.0 33.0 40.0 68-69 36.18718428632582 37.0 37.0 37.0 33.0 40.0 70-71 35.91640936249995 37.0 37.0 37.0 33.0 40.0 72-73 35.6178527966286 37.0 37.0 37.0 33.0 38.5 74-75 35.45896868501573 37.0 37.0 37.0 33.0 37.0 76-77 35.24437785089853 37.0 37.0 37.0 33.0 37.0 78-79 35.143632027210856 37.0 37.0 37.0 33.0 37.0 80-81 35.04095815418548 37.0 37.0 37.0 33.0 37.0 82-83 34.96365746330247 37.0 35.0 37.0 33.0 37.0 84-85 34.91280942673088 37.0 33.0 37.0 33.0 37.0 86-87 34.848622178333756 37.0 33.0 37.0 33.0 37.0 88-89 34.76587146844976 37.0 33.0 37.0 33.0 37.0 90-91 34.704482110042335 37.0 33.0 37.0 33.0 37.0 92-93 34.67924804153708 37.0 33.0 37.0 33.0 37.0 94-95 34.6193466063921 37.0 33.0 37.0 33.0 37.0 96-97 34.54640833336337 37.0 33.0 37.0 33.0 37.0 98-99 34.48681195512324 37.0 33.0 37.0 33.0 37.0 100-101 32.634861861439774 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.19951594267506323 2306 2 0.23548905857511215 2306 3 0.2571890155655794 2306 4 0.3234439762208865 2306 5 0.323322736305947 2306 6 0.35911533025128506 2306 7 0.31296102718940944 2306 8 0.34958996013781274 2306 9 0.3275726755893089 2306 10-11 0.3197548925343199 2306 12-13 0.34016693724861113 2306 14-15 0.5882280231521406 2306 16-17 0.6212708386094477 2306 18-19 0.5844783961533224 2306 20-21 0.6277643135526532 2306 22-23 0.6383831994796552 2306 24-25 0.6148990140568884 2306 26-27 0.5993141768501786 2306 28-29 0.6742823921431125 2306 30-31 0.635500886331748 2306 32-33 0.7160618346116294 2306 34-35 0.7091368417718016 2306 36-37 0.718218086686214 2306 38-39 0.6102802791229891 2306 40-41 0.7101615865407496 2306 42-43 0.6339612148114995 2306 44-45 0.6717268462436081 2306 46-47 0.6605997376256667 2306 48-49 0.6993024251911777 2306 50-51 0.6688083629914132 2306 52-53 0.7285218610107052 2306 54-55 0.7250855636423807 2306 56-57 0.6705010884459881 2306 58-59 0.6877712870282906 2306 60-61 0.6686691138771153 2306 62-63 0.6963706149819302 2306 64-65 0.6883460291712353 2306 66-67 0.6915300846360068 2306 68-69 0.6934659378131727 2306 70-71 0.7144336291240094 2306 72-73 0.7729881050824403 2306 74-75 0.7471909549167677 2306 76-77 0.7883774079657542 2306 78-79 0.8000377900642874 2306 80-81 0.7866682724318821 2306 82-83 0.8251547226631146 2306 84-85 0.7878817895421975 2306 86-87 0.7920164900908446 2306 88-89 0.8543559946341475 2306 90-91 0.9226654850812537 2306 92-93 0.890706981991805 2306 94-95 0.9053121378123592 2306 96-97 0.9127944742840199 2306 98-99 0.819520168721489 2306 100-101 0.907903069970672 2307 1 0.16075060738651104 2307 2 0.22905448308458887 2307 3 0.23094055959240478 2307 4 0.2658143546383869 2307 5 0.2713865809303755 2307 6 0.30214295217160014 2307 7 0.26144567515129324 2307 8 0.27472491799024823 2307 9 0.29286548275982227 2307 10-11 0.3043823892811375 2307 12-13 0.2872474526445217 2307 14-15 0.5427049312814844 2307 16-17 0.526019039564595 2307 18-19 0.5248090726753389 2307 20-21 0.5849534127232872 2307 22-23 0.5684807025912946 2307 24-25 0.5420996533240938 2307 26-27 0.5596741633420095 2307 28-29 0.5894303775006904 2307 30-31 0.5212040206943982 2307 32-33 0.6617103211586652 2307 34-35 0.5963748319796736 2307 36-37 0.6356086265081942 2307 38-39 0.6150417706222271 2307 40-41 0.6602666517272269 2307 42-43 0.5767399965802369 2307 44-45 0.6208758149893967 2307 46-47 0.6231298249168518 2307 48-49 0.615923649815592 2307 50-51 0.6240313197567815 2307 52-53 0.6578272246933068 2307 54-55 0.6645016047953476 2307 56-57 0.6206609140948061 2307 58-59 0.6220946051832144 2307 60-61 0.606812188073441 2307 62-63 0.6300531148238662 2307 64-65 0.6416536820004026 2307 66-67 0.6065365538606855 2307 68-69 0.6113133456174253 2307 70-71 0.6022215683046781 2307 72-73 0.7067444740456352 2307 74-75 0.6377740810037267 2307 76-77 0.7195829626405796 2307 78-79 0.7069707034553545 2307 80-81 0.7289079570588086 2307 82-83 0.7887325719293017 2307 84-85 0.7826021968350005 2307 86-87 0.8072481259619053 2307 88-89 0.8092617417638408 2307 90-91 0.836624118609258 2307 92-93 0.8357428856284841 2307 94-95 0.7895294412455982 2307 96-97 0.8242045494069927 2307 98-99 0.892744903535764 2307 100-101 0.9570187961374543 2201 1 0.19720361000478448 2201 2 0.22109169312365395 2201 3 0.23979309208904453 2201 4 0.23720674140850662 2201 5 0.22852413624996615 2201 6 0.2826840944057665 2201 7 0.20191519554573745 2201 8 0.23450658288716397 2201 9 0.23290023153158046 2201 10-11 0.23945687647383096 2201 12-13 0.25329401857541 2201 14-15 0.45945308173558175 2201 16-17 0.48525584795488186 2201 18-19 0.48775917977901884 2201 20-21 0.5229506673176374 2201 22-23 0.5315838024657822 2201 24-25 0.517768347210513 2201 26-27 0.5284059240569761 2201 28-29 0.5726902718072182 2201 30-31 0.5568567793631729 2201 32-33 0.6337991465017652 2201 34-35 0.5868105678750268 2201 36-37 0.6326945230790315 2201 38-39 0.615882712771068 2201 40-41 0.5884229069455813 2201 42-43 0.5957886639364247 2201 44-45 0.6134849720542803 2201 46-47 0.5886812086156752 2201 48-49 0.5418937500719849 2201 50-51 0.45469822192370657 2201 52-53 0.5855544884320238 2201 54-55 0.6482449337862093 2201 56-57 0.6782402645974912 2201 58-59 0.6955172653213921 2201 60-61 0.6808086428857578 2201 62-63 0.655010786199739 2201 64-65 0.6213813970646669 2201 66-67 0.6449081270223687 2201 68-69 0.6364782282548092 2201 70-71 0.6286983686332306 2201 72-73 0.7528692289575858 2201 74-75 0.7217507080528165 2201 76-77 0.7826233498357595 2201 78-79 0.7578447384835698 2201 80-81 0.7441629155476406 2201 82-83 0.7542928193463894 2201 84-85 0.7206299251424184 2201 86-87 0.7587032388941637 2201 88-89 0.8106338453503881 2201 90-91 0.7852580723129847 2201 92-93 0.7654771823156565 2201 94-95 0.7609242249648815 2201 96-97 0.7167031272171585 2201 98-99 0.7521014145685214 2201 100-101 0.7926306169119712 1101 1 0.12897740532743995 1101 2 0.16772739277522675 1101 3 0.17417860247310557 1101 4 0.22328505532307474 1101 5 0.23560268368413517 1101 6 0.26729769503731404 1101 7 0.223753261737329 1101 8 0.26814819001783263 1101 9 0.2800321952392011 1101 10-11 0.25494526098886894 1101 12-13 0.2581261822162162 1101 14-15 0.4766416909896094 1101 16-17 0.47317875519256347 1101 18-19 0.44832161703038054 1101 20-21 0.5145077695127327 1101 22-23 0.448850942233463 1101 24-25 0.48799118963275845 1101 26-27 0.5143031740372024 1101 28-29 0.5171199484437636 1101 30-31 0.5124930968601262 1101 32-33 0.6085529919567989 1101 34-35 0.5760175864901456 1101 36-37 0.5902735585387759 1101 38-39 0.5744405099395351 1101 40-41 0.5637592987860529 1101 42-43 0.5492828210592151 1101 44-45 0.5876501415424471 1101 46-47 0.57120709639468 1101 48-49 0.5747499904623012 1101 50-51 0.5429818545161424 1101 52-53 0.5670196506016509 1101 54-55 0.6595202526538131 1101 56-57 0.64540368609741 1101 58-59 0.6741391538862942 1101 60-61 0.6707810583318263 1101 62-63 0.6282765705188069 1101 64-65 0.6416216651579774 1101 66-67 0.5804412654904567 1101 68-69 0.6391500378559201 1101 70-71 0.6208256964470209 1101 72-73 0.7063095135278701 1101 74-75 0.6779552182419692 1101 76-77 0.7221989729359422 1101 78-79 0.6619979724343068 1101 80-81 0.6956494858708453 1101 82-83 0.6937682746130918 1101 84-85 0.6877480062207866 1101 86-87 0.6980144686077097 1101 88-89 0.766877689587318 1101 90-91 0.7582984276701765 1101 92-93 0.7017805124464189 1101 94-95 0.7174571862603472 1101 96-97 0.7393895911382131 1101 98-99 0.7934645796814905 1101 100-101 0.7900697044787037 2304 1 0.15844027984640974 2304 2 0.1850148217655132 2304 3 0.20346660125907334 2304 4 0.2272239468843722 2304 5 0.25446506800702196 2304 6 0.23730546888283044 2304 7 0.22381546681796038 2304 8 0.2685166960651131 2304 9 0.2648247352108015 2304 10-11 0.2899936721465508 2304 12-13 0.2866099888521916 2304 14-15 0.4399991046326406 2304 16-17 0.43508140030434816 2304 18-19 0.4415641696004755 2304 20-21 0.4816331002232843 2304 22-23 0.44075572765814997 2304 24-25 0.4377080813544012 2304 26-27 0.5019218933999454 2304 28-29 0.5225464648447229 2304 30-31 0.4655027060776433 2304 32-33 0.5630204119563444 2304 34-35 0.5703819064003568 2304 36-37 0.5597821169270887 2304 38-39 0.5412860609520038 2304 40-41 0.5476862289331095 2304 42-43 0.5379890913886101 2304 44-45 0.5638024387639078 2304 46-47 0.5892140355897553 2304 48-49 0.5429641606221907 2304 50-51 0.5400253594181024 2304 52-53 0.5900322018546262 2304 54-55 0.56792810323563 2304 56-57 0.5906809675707407 2304 58-59 0.5921143105085278 2304 60-61 0.6166161096420666 2304 62-63 0.5543195614593373 2304 64-65 0.5894446662918469 2304 66-67 0.5756761344088162 2304 68-69 0.5346094964641352 2304 70-71 0.535583798139804 2304 72-73 0.640425958002858 2304 74-75 0.6365747021044399 2304 76-77 0.687721610145033 2304 78-79 0.6427651135489327 2304 80-81 0.6525539992825173 2304 82-83 0.656866345325021 2304 84-85 0.6846841375658386 2304 86-87 0.7008626953692101 2304 88-89 0.6906095720891514 2304 90-91 0.7283753858775341 2304 92-93 0.6658328477496909 2304 94-95 0.6474878163570068 2304 96-97 0.6913516639346184 2304 98-99 0.6612975021320224 2304 100-101 0.7665762270643697 2202 1 0.14287094998031336 2202 2 0.17233548173682323 2202 3 0.1771385509840826 2202 4 0.23232803882603292 2202 5 0.236105506194491 2202 6 0.25057046619273393 2202 7 0.18788637338180791 2202 8 0.23882022906367695 2202 9 0.2074547940941116 2202 10-11 0.2316763112845237 2202 12-13 0.23498070337275578 2202 14-15 0.3863319746709024 2202 16-17 0.4212668005315052 2202 18-19 0.4126536229122806 2202 20-21 0.45079959875867814 2202 22-23 0.44232654081572065 2202 24-25 0.44306455702393777 2202 26-27 0.47323430746617845 2202 28-29 0.5074998364372618 2202 30-31 0.4705683146381432 2202 32-33 0.5497641014290835 2202 34-35 0.5183041774429142 2202 36-37 0.5346463042178513 2202 38-39 0.5225777201461597 2202 40-41 0.5491729764522404 2202 42-43 0.4812162965237192 2202 44-45 0.48801474373162534 2202 46-47 0.4836936319252416 2202 48-49 0.49630520931300737 2202 50-51 0.436573437490793 2202 52-53 0.5719953586874738 2202 54-55 0.5038000320376455 2202 56-57 0.5011858097515187 2202 58-59 0.4634866913968523 2202 60-61 0.5474431660744017 2202 62-63 0.5297836971898562 2202 64-65 0.540706304712451 2202 66-67 0.5622714947328191 2202 68-69 0.49785020814514525 2202 70-71 0.5123233030118683 2202 72-73 0.5828062485337 2202 74-75 0.512767967139105 2202 76-77 0.6073582224118894 2202 78-79 0.5610429448796381 2202 80-81 0.6118926642454454 2202 82-83 0.6362330882111991 2202 84-85 0.5913686635103659 2202 86-87 0.6445147121972852 2202 88-89 0.6596133569110023 2202 90-91 0.7057724708548179 2202 92-93 0.6264225028190467 2202 94-95 0.6473195916738348 2202 96-97 0.7135109926051157 2202 98-99 0.6794987403254638 2202 100-101 0.6887213579825868 1102 1 0.08926983465630656 1102 2 0.12219965029227353 1102 3 0.09774526970117847 1102 4 0.1503269150262767 1102 5 0.12230700098942293 1102 6 0.13330578228718082 1102 7 0.1317742351752429 1102 8 0.13850674820562148 1102 9 0.14780008333390526 1102 10-11 0.1499152262059411 1102 12-13 0.15846238657738354 1102 14-15 0.1819049824974499 1102 16-17 0.2022986685202639 1102 18-19 0.2439874011267662 1102 20-21 0.2609853082427023 1102 22-23 0.19940878670289663 1102 24-25 0.22052184172844846 1102 26-27 0.2630609298088942 1102 28-29 0.25850886048055344 1102 30-31 0.3179192459272997 1102 32-33 0.3600372170302677 1102 34-35 0.24165127718004697 1102 36-37 0.23310507881129894 1102 38-39 0.25869038426886704 1102 40-41 0.32447503104242514 1102 42-43 0.29010323891838397 1102 44-45 0.34712165460855005 1102 46-47 0.32293826029079753 1102 48-49 0.34297936956881614 1102 50-51 0.31629906484503323 1102 52-53 0.3613837633151462 1102 54-55 0.38832798483844044 1102 56-57 0.37846006895782835 1102 58-59 0.37768387873816067 1102 60-61 0.34910860456939474 1102 62-63 0.3457318989360516 1102 64-65 0.38366705318561856 1102 66-67 0.33488377102816 1102 68-69 0.37351520333387356 1102 70-71 0.3346357045010251 1102 72-73 0.4382787483930386 1102 74-75 0.3974767751988608 1102 76-77 0.43772101244187667 1102 78-79 0.3912510859755898 1102 80-81 0.3922760971998045 1102 82-83 0.40243818352729477 1102 84-85 0.3743077768816079 1102 86-87 0.34759556545661496 1102 88-89 0.404314704072398 1102 90-91 0.41501336149332246 1102 92-93 0.3615870503825036 1102 94-95 0.4577110026137845 1102 96-97 0.4353802715669346 1102 98-99 0.45452178972183077 1102 100-101 0.48744726767075974 2305 1 0.11425075424027398 2305 2 0.13790736671025883 2305 3 0.15446851604960443 2305 4 0.16095222187200164 2305 5 0.18428232534662214 2305 6 0.18881172352162423 2305 7 0.1425750665416956 2305 8 0.17206459933082385 2305 9 0.171251073760196 2305 10-11 0.1686072594280148 2305 12-13 0.1787238158046094 2305 14-15 0.33431980498725267 2305 16-17 0.34053707148610357 2305 18-19 0.3437206283461407 2305 20-21 0.38703932316366263 2305 22-23 0.3272845770829349 2305 24-25 0.36444485821817096 2305 26-27 0.3612754067699697 2305 28-29 0.3485463823772932 2305 30-31 0.3611976487627828 2305 32-33 0.39311751090694713 2305 34-35 0.4018384615298203 2305 36-37 0.4430879396427372 2305 38-39 0.4077962327935012 2305 40-41 0.4253027979274222 2305 42-43 0.4269582678998276 2305 44-45 0.37023293289841064 2305 46-47 0.3598991530542932 2305 48-49 0.3755379238685208 2305 50-51 0.29570182424573943 2305 52-53 0.3790895154266991 2305 54-55 0.41271725722293695 2305 56-57 0.4678380061654934 2305 58-59 0.44134578497455124 2305 60-61 0.45861213058880423 2305 62-63 0.39223257507677545 2305 64-65 0.44127051441777354 2305 66-67 0.46397758248526344 2305 68-69 0.46442926648310134 2305 70-71 0.46914143642736406 2305 72-73 0.5384877157960233 2305 74-75 0.5118611153383981 2305 76-77 0.5727095750551427 2305 78-79 0.4929830401632387 2305 80-81 0.5300697389637747 2305 82-83 0.4729724267822206 2305 84-85 0.49611502470916946 2305 86-87 0.5368502878873542 2305 88-89 0.5810441560925312 2305 90-91 0.5691571706272427 2305 92-93 0.5775776265308323 2305 94-95 0.5820255284529168 2305 96-97 0.5173754197073421 2305 98-99 0.5401016020516138 2305 100-101 0.6052528052847208 2203 1 0.08495963459309763 2203 2 0.06920202699904365 2203 3 0.10202682157323295 2203 4 0.12718721993321225 2203 5 0.1573284627658822 2203 6 0.159131083613552 2203 7 0.10347557017639275 2203 8 0.11560064745717114 2203 9 0.1468156331075008 2203 10-11 0.1442358430065056 2203 12-13 0.11717094251680749 2203 14-15 0.24886480047167225 2203 16-17 0.29616396581857174 2203 18-19 0.25236385439052356 2203 20-21 0.28780456516509645 2203 22-23 0.2326569892110868 2203 24-25 0.28147846540658605 2203 26-27 0.30991288757117275 2203 28-29 0.32638223237857034 2203 30-31 0.29872122311705596 2203 32-33 0.37834710947839767 2203 34-35 0.36181478046850657 2203 36-37 0.36275444453624317 2203 38-39 0.38620710394987157 2203 40-41 0.28807669347555986 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-1.0901452882499854 1213 80-81 -1.074817184790973 1213 82-83 -1.0906950553861634 1213 84-85 -1.065629433220039 1213 86-87 -1.1510765151919742 1213 88-89 -1.2557218981537872 1213 90-91 -1.3361181343272008 1213 92-93 -1.2368563269902282 1213 94-95 -1.1609323450004112 1213 96-97 -1.1572285433202225 1213 98-99 -1.3190392003250864 1213 100-101 -1.3734379242569332 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16608.0 3 1279.0 4 863.0 5 722.0 6 598.0 7 863.0 8 1376.0 9 3531.0 10 12444.0 11 24097.0 12 20608.0 13 16518.0 14 14466.0 15 13670.0 16 13536.0 17 14123.0 18 15831.0 19 17651.0 20 19490.0 21 21961.0 22 25131.0 23 28678.0 24 32336.0 25 36459.0 26 42679.0 27 50319.0 28 61342.0 29 76193.0 30 96457.0 31 126090.0 32 168998.0 33 233280.0 34 331125.0 35 501884.0 36 1026562.0 37 4592066.0 38 4824685.0 39 1676.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.515606989370617 15.518386449761582 18.740131443576225 38.22587511729157 2 25.063855702995042 26.839246771802962 29.42259044530194 18.674307079900057 3 22.57495085787953 28.106702049955956 29.92782810035725 19.39051899180727 4 25.084006626850808 30.92144859057509 22.193092688648374 21.80145209392573 5 27.1585740422287 33.33042914235309 22.09785278399197 17.413144031426235 6 19.877238186457458 37.59465837559735 22.487667309774253 20.040436128170942 7 18.64237926135452 17.490275070169652 42.052653227803994 21.81469244067183 8 22.286986197162246 22.727351106462248 28.16229316014415 26.823369536231358 9 24.49353487004919 23.38369344495446 27.37184461096906 24.75092707402729 10-11 24.306747881722956 31.707408131001465 22.20163588219518 21.7842081050804 12-13 23.261928295682125 25.846608379998635 27.64510850652312 23.24635481779612 14-15 23.14714365221455 27.196018162665432 27.65285310605715 22.003985079062865 16-17 24.06736884847787 27.417213839780008 26.809749869547133 21.705667442194986 18-19 23.902023682786467 27.65716256277711 26.896521871308327 21.544291883128096 20-21 23.9895734619219 27.902535072764483 26.672440995061546 21.435450470252068 22-23 24.290610677895234 27.569610795862637 26.674446897417315 21.465331628824813 24-25 23.4989301435988 28.25237369060397 26.738096882116153 21.510599283681078 26-27 23.803639827569338 28.406125966777424 26.484543575792873 21.305690629860365 28-29 23.96737003084116 27.782284396350548 26.779537467667495 21.470808105140797 30-31 23.846159984739046 27.657613087399486 26.858766914381814 21.637460013479654 32-33 23.76672413777197 28.010086076695146 26.74422825737241 21.47896152816047 34-35 23.94423181435058 27.890631380607655 26.652252961725136 21.512883843316626 36-37 23.371411324378062 27.781101507728952 27.11773189916147 21.729755268731523 38-39 23.678918544485548 27.720328533494904 26.87279183269833 21.72796108932122 40-41 24.34806776580773 27.49053035281105 26.653953391354207 21.50744849002702 42-43 24.091736299825815 27.471418269557734 26.933657516599595 21.50318791401686 44-45 24.38955428894195 27.603817652571312 26.623220429279314 21.383407629207422 46-47 24.26810777154762 27.47257759843089 26.59893618152273 21.66037844849876 48-49 23.757989246251498 27.50711596723876 26.768265834339005 21.966628952170737 50-51 24.443826245113573 27.65344441019069 26.54416449056356 21.358564854132176 52-53 24.29239308907878 27.6196743089891 26.768530427463183 21.319402174468934 54-55 24.06639523360779 27.726971587761472 26.565865937939797 21.640767240690945 56-57 24.33731861884431 27.635365625749447 26.819005047511418 21.208310707894828 58-59 24.60769583120416 27.998377148192922 26.34370204344558 21.050224977157338 60-61 24.427110819873278 28.206824158098186 26.253494513100044 21.112570508928492 62-63 24.757609077419037 28.282966011652604 25.9258128937702 21.033612017158156 64-65 25.038951103599228 28.197999725150137 25.911948403957417 20.851100767293218 66-67 24.823675543344077 28.27419749181532 26.03349848781659 20.868628477024018 68-69 25.27457092932733 28.27255438091282 25.72219934362382 20.73067534613603 70-71 25.539777296715364 28.154473178497554 25.62321983175116 20.68252969303592 72-73 25.486464025688605 28.26016852289923 25.683906982946052 20.569460468466108 74-75 25.907205304264615 28.276740388594558 25.395408390005596 20.42064591713523 76-77 26.210378238922416 28.093490985699777 25.351645431249498 20.344485344128312 78-79 26.175388228419894 28.18704872559627 25.398518038134192 20.239045007849647 80-81 26.708190637563323 28.21376525131882 24.986513128305067 20.091530982812795 82-83 26.97979178186454 28.04884681525211 25.012676613238916 19.95868478964443 84-85 26.98397525355487 28.10340419260567 25.042221371639613 19.87039918219985 86-87 27.387028949789848 28.160596155250744 24.704736225038083 19.74763866992133 88-89 27.752158187172917 28.00236651026975 24.71306163255115 19.53241367000619 90-91 27.74150485256764 28.056851246400505 24.70596385779719 19.495680043234668 92-93 28.127026884477385 28.128065708208048 24.45653375003936 19.28837365727521 94-95 28.47300906519305 27.981096597706035 24.424909281692933 19.12098505540798 96-97 28.442840858315666 28.106930075141527 24.387452560242775 19.062776506300033 98-99 28.827877745081366 28.20529253575937 24.171759659072475 18.795070060086797 100-101 29.061522494050212 28.22532919323067 24.036609722941556 18.676538589777564 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14214.0 1 7795.5 2 1094.5 3 666.0 4 490.0 5 453.5 6 445.0 7 417.0 8 402.5 9 411.5 10 403.5 11 397.0 12 399.5 13 451.5 14 510.5 15 548.0 16 630.5 17 709.0 18 773.5 19 898.0 20 1094.5 21 1313.5 22 1670.0 23 2241.0 24 3146.0 25 4654.5 26 7048.5 27 10323.0 28 14990.5 29 21983.0 30 30193.0 31 40401.5 32 53434.5 33 68190.0 34 87552.0 35 114666.0 36 152006.0 37 198265.5 38 254687.5 39 322658.0 40 402682.0 41 491193.5 42 584697.5 43 677448.0 44 760461.5 45 825562.5 46 866443.0 47 880788.0 48 866810.0 49 824440.0 50 753656.0 51 663643.5 52 562227.5 53 465310.5 54 371312.0 55 283261.5 56 213693.0 57 159216.0 58 118022.0 59 85852.5 60 60510.0 61 41730.0 62 29990.0 63 21508.5 64 14966.0 65 11187.5 66 8449.5 67 6123.0 68 4878.0 69 4004.5 70 3323.0 71 2419.5 72 1332.5 73 881.0 74 847.0 75 664.0 76 463.0 77 227.5 78 95.5 79 66.5 80 55.0 81 37.5 82 28.5 83 25.0 84 22.5 85 21.5 86 16.5 87 14.0 88 14.5 89 10.0 90 7.0 91 5.5 92 6.5 93 8.5 94 6.0 95 2.0 96 1.0 97 1.0 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16390902112292816 2 0.14922079945091357 3 0.15451464597501482 4 0.14520836812175367 5 0.17178972457181713 6 0.15317716886529484 7 0.15430641600583686 8 0.1415323082812658 9 0.16955525682563824 10-11 0.14072341493945914 12-13 0.13035596512788725 14-15 0.18986568766545775 16-17 0.19859933310347946 18-19 0.18223726283307284 20-21 0.21644704411552118 22-23 0.20122222983062496 24-25 0.19228035442342523 26-27 0.17756009737153713 28-29 0.19443072929743607 30-31 0.1423532148905251 32-33 0.1689105448056834 34-35 0.173543661619893 36-37 0.17581016474594544 38-39 0.17584220012581894 40-41 0.21026021137744524 42-43 0.19637287420226898 44-45 0.1803191444631451 46-47 0.18478407553301868 48-49 0.22521272493341646 50-51 0.20241154330842984 52-53 0.16700443970320822 54-55 0.15341342979186212 56-57 0.14948509133487023 58-59 0.16406118917732743 60-61 0.1591037141418983 62-63 0.14753894200755313 64-65 0.1698515840894684 66-67 0.17301107342949554 68-69 0.18474403130817674 70-71 0.16602335619458128 72-73 0.17487713430712878 74-75 0.20728092104920676 76-77 0.23460710008132982 78-79 0.21651912372023663 80-81 0.19282095145879108 82-83 0.16278778282735454 84-85 0.17247848523909806 86-87 0.1616224958844548 88-89 0.16484205156174478 90-91 0.17718368165802312 92-93 0.16155842512470772 94-95 0.15572798598772483 96-97 0.16326030468048913 98-99 0.15334135018714667 100-101 0.16016488610020907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2486195E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.66555488141048 #Duplication Level Percentage of deduplicated Percentage of total 1 69.73932229442761 32.54424171922985 2 14.755264518720065 13.7712521237612 3 5.797631652155679 8.1164909413762 4 2.8797986582548396 5.375496093368139 5 1.5700963747379904 3.6634709272219657 6 1.1846072173586475 3.316821186873895 7 0.7456112355188271 2.435605342191007 8 0.6026748025168154 2.249932325799333 9 0.37304609737284117 1.5667562817223486 >10 2.1809577297137763 18.182153496226377 >50 0.11963009075762791 3.7661585229500814 >100 0.04902833596623442 4.120809964021134 >500 0.0020588324062360886 0.6262047703813584 >1k 2.5497229507262065E-4 0.1886815905374364 >5k 1.7187797654202224E-5 0.07592471433965572 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.723007289250248E-4 8.008844968383082E-6 0.0 2 3.123449537669402E-4 8.008844968383082E-6 0.0 3 4.084510933875372E-4 8.008844968383082E-6 0.0 4 6.487164424390297E-4 8.008844968383082E-6 0.0 5 7.688491169647759E-4 8.008844968383082E-6 0.0 6 0.0010251321559530346 2.4026534905149247E-5 0.0 7 0.0011372559855103977 2.4026534905149247E-5 0.0 8 0.0013134505748148256 2.4026534905149247E-5 0.0 9 0.0015376982339295518 3.203537987353233E-5 0.0 10-11 0.0019341360598645143 3.6039802357723866E-5 0.0 12-13 0.0023626092656730095 4.004422484191541E-5 0.0 14-15 0.0029112151460072505 4.004422484191541E-5 0.0 16-17 0.0034838475612466407 4.8053069810298495E-5 4.004422484191541E-6 18-19 0.004112541891264712 5.205749229449004E-5 8.008844968383082E-6 20-21 0.004921435233071404 5.6061914778681574E-5 8.008844968383082E-6 22-23 0.007059796839629687 5.6061914778681574E-5 8.008844968383082E-6 24-25 0.010175237532330707 5.6061914778681574E-5 8.008844968383082E-6 26-27 0.016606340041942322 5.6061914778681574E-5 8.008844968383082E-6 28-29 0.03183916317180695 6.80751822312562E-5 8.008844968383082E-6 30-31 0.060098372642746645 7.608402719963928E-5 8.008844968383082E-6 32-33 0.10883619869784189 8.008844968383082E-5 8.008844968383082E-6 34-35 0.17504131562898065 1.0411498458898008E-4 8.008844968383082E-6 36-37 0.2722406625877619 1.0411498458898008E-4 8.008844968383082E-6 38-39 0.4026166498280701 1.1212382955736315E-4 8.008844968383082E-6 40-41 0.5744304009347924 1.2013267452574623E-4 8.008844968383082E-6 42-43 0.7950380400113886 1.361503644625124E-4 8.008844968383082E-6 44-45 1.0525344190123573 1.361503644625124E-4 8.008844968383082E-6 46-47 1.346555135491637 1.4015478694670395E-4 8.008844968383082E-6 48-49 1.6881163557032386 1.441592094308955E-4 8.008844968383082E-6 50-51 2.082315709469538 1.561724768834701E-4 8.008844968383082E-6 52-53 2.539004076101647 1.6017689936766164E-4 8.008844968383082E-6 54-55 3.050464933472527 1.7219016682023627E-4 8.008844968383082E-6 56-57 3.616397949895865 1.761945893044278E-4 8.008844968383082E-6 58-59 4.242793741408011 2.0822996917796015E-4 8.008844968383082E-6 60-61 4.933428478411558 2.1623881414634322E-4 8.008844968383082E-6 62-63 5.698209102132395 2.1623881414634322E-4 8.008844968383082E-6 64-65 6.52942309486597 2.242476591147263E-4 8.008844968383082E-6 66-67 7.422549463627631 2.242476591147263E-4 8.008844968383082E-6 68-69 8.386149663688578 2.3225650408310937E-4 8.008844968383082E-6 70-71 9.419042390415974 2.4026534905149247E-4 8.008844968383082E-6 72-73 10.532660270002191 2.4827419401987557E-4 8.008844968383082E-6 74-75 11.724656710871486 2.522786165040671E-4 8.008844968383082E-6 76-77 12.981436698690032 2.642918839566417E-4 8.008844968383082E-6 78-79 14.303264525341788 2.642918839566417E-4 8.008844968383082E-6 80-81 15.695197776424283 2.642918839566417E-4 8.008844968383082E-6 82-83 17.153828688403472 2.642918839566417E-4 8.008844968383082E-6 84-85 18.678280292755318 2.642918839566417E-4 8.008844968383082E-6 86-87 20.259782904239444 2.723007289250248E-4 1.6017689936766165E-5 88-89 21.89245803064905 2.8030957389340786E-4 1.6017689936766165E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGCC 3180 0.0 34.513836 42-43 GGCGCCC 1055 0.0 34.00194 44-45 GGGGCGC 2870 0.0 32.282047 42-43 TCGGGGG 5165 0.0 30.215382 38-39 GGGGGCG 3410 0.0 27.933352 40-41 CTCGGGG 3735 0.0 26.011782 36-37 GAGCGGC 3415 0.0 25.595266 9 GCGCCCG 1160 0.0 25.394691 44-45 GGGCCCG 730 0.0 25.05804 44-45 CGGGTAG 2135 0.0 24.925266 14-15 GGGCGGG 2580 0.0 22.831688 12-13 GGGGGGC 4190 0.0 22.676657 40-41 GATCGGG 4985 0.0 22.67188 1 AGGGTAG 4675 0.0 22.351582 28-29 GTAGGGG 3035 0.0 22.309858 18-19 CGGGGGT 1890 0.0 21.868576 38-39 GAGGGGC 2550 0.0 21.796072 9 GGGTAGA 5180 0.0 21.593763 28-29 GGGTAGG 2685 0.0 21.236238 16-17 AAGGGGC 2790 0.0 21.113005 9 >>END_MODULE