FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n02 tsrexp_lib#21.3420000000d0b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n02 tsrexp_lib#21.3420000000d0b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12486195
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC31800.034.51383642-43
GGCGCCC10550.034.0019444-45
GGGGCGC28700.032.28204742-43
TCGGGGG51650.030.21538238-39
GGGGGCG34100.027.93335240-41
CTCGGGG37350.026.01178236-37
GAGCGGC34150.025.5952669
GCGCCCG11600.025.39469144-45
GGGCCCG7300.025.0580444-45
CGGGTAG21350.024.92526614-15
GGGCGGG25800.022.83168812-13
GGGGGGC41900.022.67665740-41
GATCGGG49850.022.671881
AGGGTAG46750.022.35158228-29
GTAGGGG30350.022.30985818-19
CGGGGGT18900.021.86857638-39
GAGGGGC25500.021.7960729
GGGTAGA51800.021.59376328-29
GGGTAGG26850.021.23623816-17
AAGGGGC27900.021.1130059
Produced by FastQC (version 0.11.2)