##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l02n02 tsrexp_lib#18.3420000000d0a5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22420129 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52826975259598 33.0 33.0 33.0 33.0 33.0 2 32.43966125261813 33.0 33.0 33.0 33.0 33.0 3 32.41507303548521 33.0 33.0 33.0 33.0 33.0 4 36.27967881005502 37.0 37.0 37.0 37.0 37.0 5 36.27243402569182 37.0 37.0 37.0 37.0 37.0 6 36.345436683259045 37.0 37.0 37.0 37.0 37.0 7 36.29458719885153 37.0 37.0 37.0 37.0 37.0 8 36.359990078558425 37.0 37.0 37.0 37.0 37.0 9 36.264830501198276 37.0 37.0 37.0 37.0 37.0 10-11 36.257047562036774 37.0 37.0 37.0 37.0 37.0 12-13 36.25916385226864 37.0 37.0 37.0 37.0 37.0 14-15 38.59750693227501 40.0 40.0 40.0 37.0 40.0 16-17 38.56818598144551 40.0 40.0 40.0 37.0 40.0 18-19 38.578616563713794 40.0 40.0 40.0 37.0 40.0 20-21 38.52975275030755 40.0 40.0 40.0 37.0 40.0 22-23 38.624734117274706 40.0 40.0 40.0 37.0 40.0 24-25 38.545285399562154 40.0 40.0 40.0 37.0 40.0 26-27 38.538609300597685 40.0 40.0 40.0 37.0 40.0 28-29 38.45564334174884 40.0 40.0 40.0 37.0 40.0 30-31 38.42708246683148 40.0 40.0 40.0 37.0 40.0 32-33 38.347606942850334 40.0 40.0 40.0 37.0 40.0 34-35 38.34185976360796 40.0 40.0 40.0 37.0 40.0 36-37 38.312185536488215 40.0 40.0 40.0 37.0 40.0 38-39 38.30510720968644 40.0 40.0 40.0 37.0 40.0 40-41 38.27595539258494 40.0 37.0 40.0 37.0 40.0 42-43 38.193261176151125 40.0 37.0 40.0 37.0 40.0 44-45 38.171800594902905 40.0 37.0 40.0 37.0 40.0 46-47 38.0524060543987 40.0 37.0 40.0 37.0 40.0 48-49 37.9797544652843 40.0 37.0 40.0 37.0 40.0 50-51 36.59820666063072 38.5 37.0 38.5 35.0 40.0 52-53 36.93290109972159 38.5 37.0 40.0 33.0 40.0 54-55 37.60019750555405 40.0 37.0 40.0 33.0 40.0 56-57 37.59377517408575 40.0 37.0 40.0 33.0 40.0 58-59 37.38534818421428 40.0 37.0 40.0 33.0 40.0 60-61 37.26042278793311 37.0 37.0 40.0 33.0 40.0 62-63 37.03927858755853 37.0 37.0 40.0 33.0 40.0 64-65 36.75045458034608 37.0 37.0 40.0 33.0 40.0 66-67 36.472762311046466 37.0 37.0 40.0 33.0 40.0 68-69 36.15662777854668 37.0 37.0 37.0 33.0 40.0 70-71 35.876210614131615 37.0 37.0 37.0 33.0 40.0 72-73 35.5757501662903 37.0 37.0 37.0 33.0 40.0 74-75 35.41593676824964 37.0 37.0 37.0 33.0 37.0 76-77 35.20188409709864 37.0 37.0 37.0 33.0 37.0 78-79 35.10047234786205 37.0 37.0 37.0 33.0 37.0 80-81 34.999232698438085 37.0 37.0 37.0 33.0 37.0 82-83 34.92484996406577 37.0 37.0 37.0 33.0 37.0 84-85 34.87634551522875 37.0 35.0 37.0 33.0 37.0 86-87 34.8162464631671 37.0 33.0 37.0 33.0 37.0 88-89 34.73418257316896 37.0 33.0 37.0 33.0 37.0 90-91 34.677316531051176 37.0 33.0 37.0 33.0 37.0 92-93 34.652077626315176 37.0 33.0 37.0 33.0 37.0 94-95 34.59521858683328 37.0 33.0 37.0 33.0 37.0 96-97 34.52579119861443 37.0 33.0 37.0 33.0 37.0 98-99 34.46750968738851 37.0 33.0 37.0 33.0 37.0 100-101 32.67321894089012 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13563241249602953 2306 2 0.16412090620813302 2306 3 0.17711571930745862 2306 4 0.23875215178160403 2306 5 0.254129938679462 2306 6 0.32263240473293564 2306 7 0.1999147258575249 2306 8 0.2767377772037136 2306 9 0.21046887540266823 2306 10-11 0.23433795750089814 2306 12-13 0.2421660397408658 2306 14-15 0.443656601921532 2306 16-17 0.4791542210821831 2306 18-19 0.4630987879583799 2306 20-21 0.5289790576467155 2306 22-23 0.5614839052663143 2306 24-25 0.4667799069879308 2306 26-27 0.5481637079205512 2306 28-29 0.551560516423649 2306 30-31 0.527069969187643 2306 32-33 0.5985651985393972 2306 34-35 0.591568132516457 2306 36-37 0.6019760082429073 2306 38-39 0.5272392727154696 2306 40-41 0.6129687646465456 2306 42-43 0.5448648598936359 2306 44-45 0.6116968159320066 2306 46-47 0.5586682142957713 2306 48-49 0.5770972129365859 2306 50-51 0.52951452156438 2306 52-53 0.5902564152119467 2306 54-55 0.6045230242753306 2306 56-57 0.6125973489035133 2306 58-59 0.6412255851620614 2306 60-61 0.6487729395480955 2306 62-63 0.6414715419816375 2306 64-65 0.6557773919349543 2306 66-67 0.6460803348118489 2306 68-69 0.6214907480759777 2306 70-71 0.6059722308471933 2306 72-73 0.6876062792059869 2306 74-75 0.6409183068619981 2306 76-77 0.7049091997961483 2306 78-79 0.7033797835029887 2306 80-81 0.7145855772238789 2306 82-83 0.7393748327762637 2306 84-85 0.7240672620511646 2306 86-87 0.725300189586882 2306 88-89 0.7764778509464563 2306 90-91 0.7805291421327851 2306 92-93 0.7699595194144493 2306 94-95 0.7650943041059506 2306 96-97 0.7779583856661816 2306 98-99 0.7229105608124371 2306 100-101 0.7938818160892538 2307 1 0.15400714759245915 2307 2 0.22664282455938434 2307 3 0.23744769887682082 2307 4 0.25069906248253915 2307 5 0.24139300617317616 2307 6 0.28867554680229546 2307 7 0.23120075313266142 2307 8 0.29026151032950764 2307 9 0.2665421875142755 2307 10-11 0.2962074298550732 2307 12-13 0.2901058056437833 2307 14-15 0.5039964006597657 2307 16-17 0.43934059785598123 2307 18-19 0.4918881624092819 2307 20-21 0.5495607466599282 2307 22-23 0.5441197392962636 2307 24-25 0.49771464316501834 2307 26-27 0.5407833217211433 2307 28-29 0.5861048251337877 2307 30-31 0.5294098229977564 2307 32-33 0.6317921721261897 2307 34-35 0.6118738905939693 2307 36-37 0.6114686610816875 2307 38-39 0.5601300739958432 2307 40-41 0.5918332135094388 2307 42-43 0.5430842974641976 2307 44-45 0.5915654435042157 2307 46-47 0.582043320867669 2307 48-49 0.5826825153929462 2307 50-51 0.558963641635124 2307 52-53 0.6482638416039492 2307 54-55 0.619824488304296 2307 56-57 0.6358836650462791 2307 58-59 0.5850903951095034 2307 60-61 0.5900732179728578 2307 62-63 0.6331842361035527 2307 64-65 0.6231307253687319 2307 66-67 0.5975722634033573 2307 68-69 0.5895671597996355 2307 70-71 0.565343803466483 2307 72-73 0.637509279948425 2307 74-75 0.5628329953135847 2307 76-77 0.6305697377237962 2307 78-79 0.6154001580229931 2307 80-81 0.6476332294729659 2307 82-83 0.6733282876799223 2307 84-85 0.6768478727983975 2307 86-87 0.6886383408444132 2307 88-89 0.7525856401375037 2307 90-91 0.7417579621864334 2307 92-93 0.7459557360797291 2307 94-95 0.7001542883013556 2307 96-97 0.7024616606250476 2307 98-99 0.7581738433982323 2307 100-101 0.7924608315182127 2201 1 0.18692533002998601 2201 2 0.19034082035309297 2201 3 0.21899257943501027 2201 4 0.24538665617580335 2201 5 0.25334013916045706 2201 6 0.27967013372309424 2201 7 0.21439943325996325 2201 8 0.2439661590351676 2201 9 0.2715329848643151 2201 10-11 0.2550796212221229 2201 12-13 0.2916415334775664 2201 14-15 0.4957508236444639 2201 16-17 0.48362353827517524 2201 18-19 0.48699752986659917 2201 20-21 0.5377686347390238 2201 22-23 0.5355413178646486 2201 24-25 0.5225686241627372 2201 26-27 0.5504399098756778 2201 28-29 0.5895606722516575 2201 30-31 0.5679849908735477 2201 32-33 0.6333877885385064 2201 34-35 0.6077958782426549 2201 36-37 0.6160409452925961 2201 38-39 0.6125306052744648 2201 40-41 0.5635154812502279 2201 42-43 0.5745550231052263 2201 44-45 0.5881460729709502 2201 46-47 0.5959089649175269 2201 48-49 0.6203343167026603 2201 50-51 0.5349194487683633 2201 52-53 0.6233069211749509 2201 54-55 0.6163913683607447 2201 56-57 0.6568349386737751 2201 58-59 0.7030505269447858 2201 60-61 0.6669127604992227 2201 62-63 0.6322351070659522 2201 64-65 0.6361588306301869 2201 66-67 0.6346268931859029 2201 68-69 0.6183911239613948 2201 70-71 0.6453752636370709 2201 72-73 0.7328130831537081 2201 74-75 0.6770774152930343 2201 76-77 0.7085934791380311 2201 78-79 0.674527738500565 2201 80-81 0.6659429550865141 2201 82-83 0.6594904826513357 2201 84-85 0.6966528158527723 2201 86-87 0.7373887527302614 2201 88-89 0.7596516176017261 2201 90-91 0.7177553444966591 2201 92-93 0.7013398761349592 2201 94-95 0.7558596750216466 2201 96-97 0.7148340489108733 2201 98-99 0.7294558451285695 2201 100-101 0.7957976923352135 1101 1 0.16559988918647406 1101 2 0.17079828892183713 1101 3 0.21539525081734467 1101 4 0.2469025890973242 1101 5 0.2807946685215157 1101 6 0.30555875900035545 1101 7 0.24161861780429916 1101 8 0.2872680638770859 1101 9 0.30276714796701043 1101 10-11 0.2788926616536074 1101 12-13 0.28708545398237817 1101 14-15 0.48416515664710147 1101 16-17 0.4921066162544321 1101 18-19 0.49772414096447903 1101 20-21 0.528828395904398 1101 22-23 0.5149628885413051 1101 24-25 0.5089609963236299 1101 26-27 0.5163565696202213 1101 28-29 0.5648464194738878 1101 30-31 0.5137575725012269 1101 32-33 0.6052820245233832 1101 34-35 0.5712770675700796 1101 36-37 0.5859748383918486 1101 38-39 0.6091945856864598 1101 40-41 0.5827651257487858 1101 42-43 0.571979475705561 1101 44-45 0.6121610341792021 1101 46-47 0.5930753921025982 1101 48-49 0.584350057661787 1101 50-51 0.5585990583101932 1101 52-53 0.6190767870097673 1101 54-55 0.6376857725957379 1101 56-57 0.6369768182028892 1101 58-59 0.6488693301051924 1101 60-61 0.6681714745701512 1101 62-63 0.642494107815061 1101 64-65 0.6582051574680037 1101 66-67 0.6202586210913381 1101 68-69 0.6144955133895635 1101 70-71 0.6160493393422755 1101 72-73 0.6819760253198126 1101 74-75 0.6385999429740821 1101 76-77 0.6564958503420897 1101 78-79 0.6370745459577165 1101 80-81 0.6848634817673727 1101 82-83 0.6561079941840973 1101 84-85 0.673971830161058 1101 86-87 0.7527792541343175 1101 88-89 0.7404718359425857 1101 90-91 0.7682715293961522 1101 92-93 0.7072261635934964 1101 94-95 0.7218811559108005 1101 96-97 0.737152790277662 1101 98-99 0.7288993872004141 1101 100-101 0.7028356365643234 2304 1 0.17410467158761378 2304 2 0.21083072937960878 2304 3 0.2142679501120952 2304 4 0.2640168033343997 2304 5 0.28295493804713345 2304 6 0.25730574562551567 2304 7 0.24006552308554774 2304 8 0.29059941866849215 2304 9 0.2823685714767663 2304 10-11 0.2973059334561938 2304 12-13 0.28633300980682463 2304 14-15 0.46031002753293393 2304 16-17 0.4792531599705825 2304 18-19 0.4573159336049599 2304 20-21 0.511989590888227 2304 22-23 0.48439458922207024 2304 24-25 0.46182613658248783 2304 26-27 0.5100660721530517 2304 28-29 0.49474837847316167 2304 30-31 0.46166591034740634 2304 32-33 0.5592022859891657 2304 34-35 0.5521363217967661 2304 36-37 0.5743407170924897 2304 38-39 0.5712580817960529 2304 40-41 0.5666667133162591 2304 42-43 0.5725298606368767 2304 44-45 0.5630840907472958 2304 46-47 0.5697088726734378 2304 48-49 0.5897660343964972 2304 50-51 0.5586634779705548 2304 52-53 0.580614218127117 2304 54-55 0.5752142217223266 2304 56-57 0.5831103207093591 2304 58-59 0.5986318790637029 2304 60-61 0.6075357973149096 2304 62-63 0.5711985807590665 2304 64-65 0.6152237618312384 2304 66-67 0.5844466654613072 2304 68-69 0.5554424095670996 2304 70-71 0.5534916932834832 2304 72-73 0.6039059863229426 2304 74-75 0.5812701734438761 2304 76-77 0.6399033659922537 2304 78-79 0.6431050238267417 2304 80-81 0.6581100748516988 2304 82-83 0.6884571546389893 2304 84-85 0.6646025844710621 2304 86-87 0.677275033736187 2304 88-89 0.6870918672836623 2304 90-91 0.7023964875840605 2304 92-93 0.6283568179359733 2304 94-95 0.6146493424132586 2304 96-97 0.6451737402795175 2304 98-99 0.630011133548912 2304 100-101 0.7053051724809336 2202 1 0.14270116194177973 2202 2 0.1838544768659176 2202 3 0.19386327742999043 2202 4 0.2289065408342168 2202 5 0.2426176051100839 2202 6 0.25944749631629094 2202 7 0.21599935187891361 2202 8 0.23817703090224285 2202 9 0.24604716962655715 2202 10-11 0.2635870543948826 2202 12-13 0.2710304779377637 2202 14-15 0.41176894736156555 2202 16-17 0.4160563185795567 2202 18-19 0.422702060639466 2202 20-21 0.475484078796903 2202 22-23 0.4343791050787189 2202 24-25 0.4558781956007749 2202 26-27 0.47918750459388804 2202 28-29 0.495837559032168 2202 30-31 0.4795329139276774 2202 32-33 0.5403553796614275 2202 34-35 0.5055238818633399 2202 36-37 0.47332458809121647 2202 38-39 0.5412430254526228 2202 40-41 0.5458910202437508 2202 42-43 0.5002271399693541 2202 44-45 0.5280750726011902 2202 46-47 0.5342146402075443 2202 48-49 0.5537784239250598 2202 50-51 0.47751181029804 2202 52-53 0.5699104052089012 2202 54-55 0.5777312415013611 2202 56-57 0.5700206685937204 2202 58-59 0.5445007740796157 2202 60-61 0.5625189469140324 2202 62-63 0.5575863469444542 2202 64-65 0.557651056924918 2202 66-67 0.5604902604222914 2202 68-69 0.556665676426384 2202 70-71 0.5432633194242484 2202 72-73 0.6053765160033819 2202 74-75 0.5294109741844011 2202 76-77 0.5673944044140597 2202 78-79 0.6220866828980078 2202 80-81 0.6468617611859244 2202 82-83 0.6463647350745774 2202 84-85 0.5998967960548498 2202 86-87 0.6127839103729826 2202 88-89 0.6281848189282968 2202 90-91 0.6821310452195704 2202 92-93 0.6300450974519833 2202 94-95 0.6013911016404947 2202 96-97 0.665472928048942 2202 98-99 0.6252326181689654 2202 100-101 0.7261344544936392 1102 1 0.08205088523200033 1102 2 0.13245254523916117 1102 3 0.12239630431160009 1102 4 0.16297554909976952 1102 5 0.138778990297304 1102 6 0.1632626282384635 1102 7 0.12543091854668376 1102 8 0.14231211720890258 1102 9 0.13169378477885374 1102 10-11 0.14723430321830477 1102 12-13 0.14934276259002388 1102 14-15 0.16790021696466084 1102 16-17 0.21363854095506696 1102 18-19 0.2565411261525128 1102 20-21 0.27924117617953925 1102 22-23 0.213393626005427 1102 24-25 0.2327190203125511 1102 26-27 0.28195464525414593 1102 28-29 0.26079289781537085 1102 30-31 0.30022709508141787 1102 32-33 0.3626710482960718 1102 34-35 0.2538845712353961 1102 36-37 0.28902470448440454 1102 38-39 0.2947687521517679 1102 40-41 0.30604063438745044 1102 42-43 0.30673423060982685 1102 44-45 0.3349628612595765 1102 46-47 0.3406735848069431 1102 48-49 0.3456042620884361 1102 50-51 0.351874755862184 1102 52-53 0.38199818645550465 1102 54-55 0.380019141617872 1102 56-57 0.34072419092334627 1102 58-59 0.3744316322551242 1102 60-61 0.3838125349657844 1102 62-63 0.3530807874618418 1102 64-65 0.39547076593154884 1102 66-67 0.37373561975363145 1102 68-69 0.38556328129995876 1102 70-71 0.36062472802098 1102 72-73 0.4735908396982751 1102 74-75 0.45224934505642267 1102 76-77 0.494351845527504 1102 78-79 0.4392511532111385 1102 80-81 0.4291380678482639 1102 82-83 0.4174149663841007 1102 84-85 0.39992131855258606 1102 86-87 0.39651584437064713 1102 88-89 0.4452907287829859 1102 90-91 0.44553653156888373 1102 92-93 0.408255592334946 1102 94-95 0.4690210988561816 1102 96-97 0.4557869450536103 1102 98-99 0.4654671633234173 1102 100-101 0.5122406493841467 2305 1 0.09461724658780923 2305 2 0.12809324257202093 2305 3 0.12685283973451789 2305 4 0.12033826924240287 2305 5 0.17355331307116018 2305 6 0.16463780645586468 2305 7 0.13245010001064372 2305 8 0.15436242850724113 2305 9 0.17626507532363433 2305 10-11 0.17066678325743823 2305 12-13 0.17631932540187023 2305 14-15 0.2960523749689088 2305 16-17 0.3056156104007428 2305 18-19 0.31306375528528463 2305 20-21 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1213 68-69 -1.1833259419711197 1213 70-71 -1.1015582875637264 1213 72-73 -0.9566983308286936 1213 74-75 -1.0877694957316564 1213 76-77 -0.9835893476425994 1213 78-79 -1.091820866196933 1213 80-81 -1.0478840572828787 1213 82-83 -1.094013759710009 1213 84-85 -1.0578201956920523 1213 86-87 -1.1148223046824839 1213 88-89 -1.2283167674845359 1213 90-91 -1.3247331755998673 1213 92-93 -1.1919651780703546 1213 94-95 -1.1607074586848114 1213 96-97 -1.2268867010421545 1213 98-99 -1.3063008125419628 1213 100-101 -1.3527099888227738 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30525.0 3 2359.0 4 1557.0 5 1282.0 6 1060.0 7 1853.0 8 3323.0 9 8523.0 10 31572.0 11 59449.0 12 49530.0 13 37321.0 14 31815.0 15 28585.0 16 27466.0 17 28430.0 18 30902.0 19 34018.0 20 37307.0 21 40899.0 22 46619.0 23 52678.0 24 59294.0 25 65494.0 26 75504.0 27 89178.0 28 107493.0 29 133553.0 30 168800.0 31 219354.0 32 292727.0 33 405591.0 34 575121.0 35 867226.0 36 1759186.0 37 8064861.0 38 8946423.0 39 3251.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.053294719494552 15.882405161247892 19.65256659298906 37.41173352626849 2 24.794945638308914 27.53061046079332 28.82603789806375 18.848406002834018 3 22.360875453729033 28.479403037268934 30.176198730735855 18.983522778266178 4 24.726685287733904 31.091699896769665 22.069203187767094 22.11241162772934 5 27.08218148698787 33.60106956277662 21.79404393082014 17.522705019415362 6 19.999403172113414 38.028483610887335 22.057038787513772 19.915074429485475 7 18.74808686837172 18.361078097584592 41.26911054322485 21.621724490818846 8 21.966900207806695 23.3951753631123 27.936012708444878 26.701911720636122 9 24.443709804980923 23.71272169519362 27.10642687355441 24.737141626271054 10-11 24.113686127649636 32.21575212953339 22.06304311747479 21.607518625342177 12-13 23.217797147028367 26.253454200887106 27.32548916200926 23.20325949007527 14-15 22.97704207162232 27.552416635447337 27.45041064500025 22.020130647930092 16-17 24.00908851130856 27.73910599545476 26.62834505442195 21.62346043881473 18-19 23.849347760893966 27.998226233873215 26.73750231976203 21.41492368547079 20-21 23.890329866701904 28.336786226501086 26.459195662595192 21.313688244201813 22-23 24.33245314459255 27.88922730727298 26.445216748404864 21.33310279972961 24-25 23.322637887744165 28.736173194088305 26.529709636461362 21.411479281706168 26-27 23.662812632940906 28.856453905139556 26.28789345732516 21.192840004594373 28-29 23.871878149698652 28.136815228069885 26.594580873116435 21.396725749115024 30-31 23.842127002310356 27.912702075819173 26.703475531895077 21.541695389975395 32-33 23.594490102650877 28.35357864390681 26.64905144214188 21.40287981130043 34-35 23.836008126824794 28.223444385089568 26.467735758938364 21.472811729147274 36-37 23.136334849232174 28.006284151485506 27.086715709572186 21.770665289710138 38-39 23.446683974202326 27.909945050115848 26.850969982055734 21.792400993626096 40-41 24.325412624367726 27.6378929763599 26.536632334699377 21.50006206457299 42-43 24.05164858493201 27.6316465737813 26.846978246053116 21.469726595233574 44-45 24.296597878408964 27.77003035600123 26.540735860785208 21.392635904804603 46-47 24.117182417887076 27.597529534948706 26.526046269673113 21.759241777491106 48-49 23.445060620645325 27.60429021124172 26.709813703759107 22.240835464353847 50-51 24.219538603836842 27.73520696316799 26.582826107200415 21.46242832579475 52-53 23.919978387873027 27.782551853277194 26.894567853185336 21.402901905664443 54-55 23.641624819715005 27.887846186226977 26.628905143740283 21.841623850317735 56-57 23.881086593945078 27.70780029393856 27.088323019098056 21.322790093018305 58-59 24.05699744282912 28.283837479093087 26.465863046512844 21.19330203156495 60-61 23.84813326568916 28.566046170184894 26.32411410468537 21.26170645944057 62-63 24.13838443642228 28.603337091879066 26.050894122025785 21.207384349672868 64-65 24.34442127449035 28.490143965012837 26.083304199926577 21.082130560570235 66-67 24.108947263618727 28.557149546326745 26.229467067495136 21.104436122559395 68-69 24.52411864540348 28.504927769108896 25.916709750419002 21.054243835068625 70-71 24.721247071018652 28.362808804510507 25.87538149555528 21.040562628915556 72-73 24.64271293000174 28.41961968646003 25.97387913099456 20.963788252543676 74-75 25.03692103611595 28.45526523855117 25.67568926605213 20.83212445928075 76-77 25.299751456291407 28.249528306600297 25.636214643170298 20.814505593938 78-79 25.20798847021967 28.34073985796799 25.71387362989203 20.737398041920304 80-81 25.70599523858194 28.3859565026213 25.30139626164641 20.60665199715035 82-83 25.94433642528256 28.256011519528784 25.318422221288966 20.48122983389969 84-85 25.87293425495023 28.30461122113167 25.3983058413007 20.4241486826174 86-87 26.27187616186709 28.348328390181294 25.056701124425985 20.32309432352563 88-89 26.628170105618025 28.196398763860252 25.07348789056547 20.101943239956253 90-91 26.594437002300992 28.28623521110904 25.043413827414902 20.07591395917506 92-93 26.997156010838054 28.345273664848 24.78089734598614 19.876672978327804 94-95 27.296623782630842 28.20725014315092 24.756050394931243 19.740075679286992 96-97 27.28877286335726 28.329412840110844 24.713055503792127 19.66875879273977 98-99 27.712173836439685 28.437214542109462 24.466088764091523 19.384522857359325 100-101 27.942636970924983 28.397115899330693 24.34128399494879 19.318963134795535 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 26167.0 1 14327.0 2 1978.0 3 1251.5 4 957.5 5 852.5 6 792.5 7 766.5 8 791.0 9 790.0 10 795.5 11 808.5 12 817.5 13 917.0 14 1030.5 15 1147.5 16 1299.5 17 1469.0 18 1683.0 19 1931.5 20 2335.5 21 2884.0 22 3631.5 23 4766.5 24 6707.0 25 9781.5 26 14572.0 27 20751.0 28 29663.0 29 42995.0 30 57449.5 31 75331.0 32 99907.5 33 128813.0 34 165491.5 35 217080.5 36 289804.0 37 381083.0 38 490930.0 39 624147.0 40 781121.5 41 948866.5 42 1120660.5 43 1285360.5 44 1419947.5 45 1518939.5 46 1575296.0 47 1576746.5 48 1527631.5 49 1431565.0 50 1292060.5 51 1126198.0 52 944896.5 53 775744.0 54 615951.5 55 468914.0 56 353494.0 57 263522.0 58 195096.5 59 141872.0 60 99505.0 61 67347.0 62 47813.0 63 34164.0 64 23415.0 65 17164.0 66 12850.0 67 9431.5 68 7585.5 69 5956.5 70 4637.0 71 3330.5 72 1948.0 73 1308.0 74 1229.0 75 980.0 76 721.0 77 402.0 78 200.5 79 133.0 80 98.5 81 75.0 82 60.0 83 50.5 84 40.0 85 31.0 86 31.5 87 28.5 88 20.5 89 19.5 90 15.0 91 6.5 92 7.0 93 7.0 94 4.0 95 1.0 96 2.0 97 1.5 98 1.0 99 2.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16717120583918138 2 0.1527020651843707 3 0.15766189391684587 4 0.14852278503839117 5 0.1757126375142623 6 0.1566271095050345 7 0.15773771863667688 8 0.14494118209578544 9 0.17296956676743475 10-11 0.1445999708565459 12-13 0.1338127893911761 14-15 0.1940309977699058 16-17 0.2019948234909799 18-19 0.18624335301549783 20-21 0.22085956775717033 22-23 0.20495198756438912 24-25 0.19597567881968922 26-27 0.1810404391517997 28-29 0.19837753832727725 30-31 0.14575295262574092 32-33 0.17275547344085307 34-35 0.17697712622438524 36-37 0.17991644918724597 38-39 0.17966890377838593 40-41 0.21528868098841 42-43 0.20017280007621724 44-45 0.18446816251592488 46-47 0.1887500290475581 48-49 0.22902187583309624 50-51 0.2062097858580564 52-53 0.1706078497585808 54-55 0.1564910710371024 56-57 0.15235639366749407 58-59 0.1676216938805303 60-61 0.1620552673894071 62-63 0.15080644718859557 64-65 0.17347134800161051 66-67 0.1768477781729088 68-69 0.1882393272581081 70-71 0.16943479674001877 72-73 0.17848246992691255 74-75 0.2113368750019235 76-77 0.2392515225938263 78-79 0.2209532335875498 80-81 0.1963503421412071 82-83 0.16623008725774951 84-85 0.17601593639358631 86-87 0.16491876563243682 88-89 0.1689107141176574 90-91 0.1804204605602403 92-93 0.16483625049615014 94-95 0.1585026562514426 96-97 0.16643972030669404 98-99 0.15629258868225065 100-101 0.1631703367986866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.2420129E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.55336428446638 #Duplication Level Percentage of deduplicated Percentage of total 1 62.75584277415287 24.19448867450613 2 16.641336707365774 12.8315903251907 3 7.1256189548418645 8.24149749954951 4 4.218180562050154 6.50500207305499 5 2.125572397659634 4.097398347998925 6 1.363617366443963 3.1543222243883275 7 1.0493610757986944 2.831947987484462 8 0.7612688206604877 2.3479579329063913 9 0.5836996025502119 2.025322506883491 >10 3.089278134421747 21.11070537599539 >50 0.1927738183267103 5.1014398896783355 >100 0.08881188425191806 5.99190397739678 >500 0.003950034461768856 1.023249471387626 >1k 6.645615977521717E-4 0.37708182036434895 >5k 0.0 0.0 >10k+ 2.330541662394418E-5 0.16609189321442158 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 24950 0.11128392704609327 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.3193443713013427E-4 4.460277637117967E-6 0.0 2 2.497755476786061E-4 4.460277637117967E-6 0.0 3 3.032988793240217E-4 4.460277637117967E-6 0.0 4 5.084716506314482E-4 4.460277637117967E-6 0.0 5 5.44153871728392E-4 4.460277637117967E-6 0.0 6 6.467402573821051E-4 8.920555274235933E-6 0.0 7 7.225649772131106E-4 8.920555274235933E-6 0.0 8 8.519130286895316E-4 8.920555274235933E-6 0.0 9 9.500391367061268E-4 8.920555274235933E-6 0.0 10-11 0.0012600284324858256 1.5610971729912883E-5 0.0 12-13 0.0016436123092779707 2.230138818558983E-5 0.0 14-15 0.0019625221603319053 2.230138818558983E-5 0.0 16-17 0.0023617170088539633 2.230138818558983E-5 2.2301388185589833E-6 18-19 0.002966084628683448 2.67616658227078E-5 4.460277637117967E-6 20-21 0.0037823154362760358 3.1221943459825765E-5 6.69041645567695E-6 22-23 0.006079358419391789 4.01424987340617E-5 8.920555274235933E-6 24-25 0.009288528179298166 4.01424987340617E-5 8.920555274235933E-6 26-27 0.015229617991939295 4.01424987340617E-5 1.1150694092794918E-5 28-29 0.028358445216796032 4.906305400829763E-5 1.33808329113539E-5 30-31 0.05138685865723609 5.35233316454156E-5 1.33808329113539E-5 32-33 0.08920332260354077 5.575347046397459E-5 1.5610971729912883E-5 34-35 0.13680117540804515 6.69041645567695E-5 1.7841110548471867E-5 36-37 0.20217323459646463 7.136444219388747E-5 1.7841110548471867E-5 38-39 0.28557819627175207 7.582471983100543E-5 1.7841110548471867E-5 40-41 0.3922055934646942 8.697541392380035E-5 1.7841110548471867E-5 42-43 0.5262034843778107 8.920555274235933E-5 1.7841110548471867E-5 44-45 0.6801298957735702 8.920555274235933E-5 1.7841110548471867E-5 46-47 0.8568706272831883 9.143569156091832E-5 1.7841110548471867E-5 48-49 1.0576210333134122 9.36658303794773E-5 1.7841110548471867E-5 50-51 1.2936745368414249 9.589596919803628E-5 1.7841110548471867E-5 52-53 1.5773192027574863 1.0035624683515424E-4 1.7841110548471867E-5 54-55 1.8988762285890504 1.070466632908312E-4 2.230138818558983E-5 56-57 2.2613451510470792 1.070466632908312E-4 2.230138818558983E-5 58-59 2.6690725106889435 1.1596721856506714E-4 2.67616658227078E-5 60-61 3.1270025252753895 1.1596721856506714E-4 2.67616658227078E-5 62-63 3.6458911543283268 1.1819735738362612E-4 2.67616658227078E-5 64-65 4.2206670621743525 1.204274962021851E-4 2.67616658227078E-5 66-67 4.851450676309668 1.2488777383930306E-4 2.67616658227078E-5 68-69 5.542109057445655 1.2934805147642105E-4 2.67616658227078E-5 70-71 6.30051013533419 1.33808329113539E-4 2.67616658227078E-5 72-73 7.131836752589604 1.33808329113539E-4 2.67616658227078E-5 74-75 8.0412204586334 1.4272888438777493E-4 2.67616658227078E-5 76-77 9.010588208479977 1.471891620248929E-4 2.67616658227078E-5 78-79 10.044634444342403 1.471891620248929E-4 2.67616658227078E-5 80-81 11.1499469962907 1.471891620248929E-4 2.8991804641266784E-5 82-83 12.327322023883092 1.5387957848056986E-4 3.5682221096943733E-5 84-85 13.577535615428438 1.5610971729912885E-4 3.7912359915502714E-5 86-87 14.88478946753607 1.605699949362468E-4 4.01424987340617E-5 88-89 16.243363274136378 1.6503027257336477E-4 4.01424987340617E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGCC 7695 0.0 38.18961 42-43 GGCGCCC 2560 0.0 37.95537 44-45 GGGGCGC 6835 0.0 36.98173 42-43 TCGGGGG 11410 0.0 35.744648 38-39 GAGCGGC 6505 0.0 32.496788 9 AAGGGGC 5585 0.0 31.810913 9 GATCGGG 9845 0.0 31.40023 1 GGGGGCG 8235 0.0 31.20959 40-41 GCGCCCG 2610 0.0 29.764404 44-45 CGGGTAG 4520 0.0 29.537962 14-15 CTCGGGG 8610 0.0 29.271362 36-37 GTAGGGG 6355 0.0 29.00971 18-19 GGGCGGG 5625 0.0 28.376068 12-13 GGGCCCG 1725 0.0 27.957407 44-45 GAGGGGC 4935 0.0 27.915058 9 AGGGTAG 9930 0.0 27.311993 28-29 GGGTAGA 11195 0.0 25.54106 28-29 AGAGCGG 8845 0.0 24.911247 8 GGGGGTC 4405 0.0 24.804832 40-41 GGGGGGC 10250 0.0 24.749807 40-41 >>END_MODULE