FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n02 tsrexp_lib#18.3420000000d0a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n02 tsrexp_lib#18.3420000000d0a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22420129
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG249500.11128392704609327Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC76950.038.1896142-43
GGCGCCC25600.037.9553744-45
GGGGCGC68350.036.9817342-43
TCGGGGG114100.035.74464838-39
GAGCGGC65050.032.4967889
AAGGGGC55850.031.8109139
GATCGGG98450.031.400231
GGGGGCG82350.031.2095940-41
GCGCCCG26100.029.76440444-45
CGGGTAG45200.029.53796214-15
CTCGGGG86100.029.27136236-37
GTAGGGG63550.029.0097118-19
GGGCGGG56250.028.37606812-13
GGGCCCG17250.027.95740744-45
GAGGGGC49350.027.9150589
AGGGTAG99300.027.31199328-29
GGGTAGA111950.025.5410628-29
AGAGCGG88450.024.9112478
GGGGGTC44050.024.80483240-41
GGGGGGC102500.024.74980740-41
Produced by FastQC (version 0.11.2)