FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n02 tsrexp_lib#15.3420000000d098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n02 tsrexp_lib#15.3420000000d098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21077441
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC15900.035.5612444-45
GGGCGCC48200.033.61445642-43
GGGGCGC46000.031.24541942-43
TCGGGGG75650.030.23788538-39
GGGGGCG51350.028.54146240-41
CTCGGGG59000.024.31785236-37
GGGGGGC61650.024.27386540-41
GCGCCCG19350.023.94146544-45
GGGCCCG10500.023.53103844-45
GAGCGGC53750.023.4198979
CGGGTAG31900.023.08587814-15
GTAGGGG45950.023.00701118-19
AAGGGGC42750.022.8901569
GGGCGGG37750.022.46246712-13
AGGGTAG67950.021.70386528-29
GGGTAGA73000.021.47118628-29
GATCGGG81450.021.2213021
CGGGGGT27250.021.00799638-39
CGGGGGG71950.020.70002438-39
GGGGCCC18500.019.64792844-45
Produced by FastQC (version 0.11.2)