##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l02n02 tsrexp_lib#10.3420000000d08b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9509734 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56798833700291 33.0 33.0 33.0 33.0 33.0 2 32.52429573739917 33.0 33.0 33.0 33.0 33.0 3 32.511314301745976 33.0 33.0 33.0 33.0 33.0 4 36.3892603094892 37.0 37.0 37.0 37.0 37.0 5 36.37577328661349 37.0 37.0 37.0 37.0 37.0 6 36.38516482164486 37.0 37.0 37.0 37.0 37.0 7 36.41950563496308 37.0 37.0 37.0 37.0 37.0 8 36.420729854273524 37.0 37.0 37.0 37.0 37.0 9 36.39858443990126 37.0 37.0 37.0 37.0 37.0 10-11 36.37869608129944 37.0 37.0 37.0 37.0 37.0 12-13 36.38196804453206 37.0 37.0 37.0 37.0 37.0 14-15 38.72936708850111 40.0 40.0 40.0 37.0 40.0 16-17 38.70249504349964 40.0 40.0 40.0 37.0 40.0 18-19 38.70457223093727 40.0 40.0 40.0 37.0 40.0 20-21 38.652182647800664 40.0 40.0 40.0 37.0 40.0 22-23 38.66095760407178 40.0 40.0 40.0 37.0 40.0 24-25 38.63999129733807 40.0 40.0 40.0 37.0 40.0 26-27 38.616487485349225 40.0 40.0 40.0 37.0 40.0 28-29 38.57681518747002 40.0 40.0 40.0 37.0 40.0 30-31 38.55787264922447 40.0 40.0 40.0 37.0 40.0 32-33 38.47879204612873 40.0 40.0 40.0 37.0 40.0 34-35 38.46470258789573 40.0 40.0 40.0 37.0 40.0 36-37 38.426864673607064 40.0 40.0 40.0 37.0 40.0 38-39 38.39963967446408 40.0 40.0 40.0 37.0 40.0 40-41 38.34742012762923 40.0 37.0 40.0 37.0 40.0 42-43 38.29473579387184 40.0 37.0 40.0 37.0 40.0 44-45 38.23780323403368 40.0 37.0 40.0 37.0 40.0 46-47 38.15043691022272 40.0 37.0 40.0 37.0 40.0 48-49 38.03913521661069 40.0 37.0 40.0 37.0 40.0 50-51 36.663719773865395 38.5 37.0 38.5 35.0 40.0 52-53 36.97857942188499 38.5 37.0 40.0 33.0 40.0 54-55 37.63062804911263 40.0 37.0 40.0 33.0 40.0 56-57 37.57593440573627 40.0 37.0 40.0 33.0 40.0 58-59 37.375467915296056 38.5 37.0 40.0 33.0 40.0 60-61 37.17536273885263 37.0 37.0 40.0 33.0 40.0 62-63 36.946834948275104 37.0 37.0 40.0 33.0 40.0 64-65 36.67196947885188 37.0 37.0 40.0 33.0 40.0 66-67 36.43744662048381 37.0 37.0 40.0 33.0 40.0 68-69 36.17464247685582 37.0 37.0 37.0 33.0 40.0 70-71 35.9321174493419 37.0 37.0 37.0 33.0 40.0 72-73 35.6515778990243 37.0 37.0 37.0 33.0 38.5 74-75 35.50382003324172 37.0 37.0 37.0 33.0 37.0 76-77 35.29615050221173 37.0 37.0 37.0 33.0 37.0 78-79 35.19944495818705 37.0 37.0 37.0 33.0 37.0 80-81 35.10217146978033 37.0 37.0 37.0 33.0 37.0 82-83 35.024780188383815 37.0 37.0 37.0 33.0 37.0 84-85 34.97323111245804 37.0 33.0 37.0 33.0 37.0 86-87 34.91011515148583 37.0 33.0 37.0 33.0 37.0 88-89 34.830434005830234 37.0 33.0 37.0 33.0 37.0 90-91 34.76998599540218 37.0 33.0 37.0 33.0 37.0 92-93 34.74217070635204 37.0 33.0 37.0 33.0 37.0 94-95 34.68028637814685 37.0 33.0 37.0 33.0 37.0 96-97 34.605854275208955 37.0 33.0 37.0 33.0 37.0 98-99 34.543289591485944 37.0 33.0 37.0 33.0 37.0 100-101 32.707284136443775 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11568842566920523 2306 2 0.1599866866934221 2306 3 0.18273317601511252 2306 4 0.24342082810932197 2306 5 0.22983325407562205 2306 6 0.28455265757431647 2306 7 0.20610514170211758 2306 8 0.24168447865573484 2306 9 0.24557128877194856 2306 10-11 0.25241221198323416 2306 12-13 0.2346881452796552 2306 14-15 0.48796259564841193 2306 16-17 0.5336308251346011 2306 18-19 0.4993164079953871 2306 20-21 0.5541175671245355 2306 22-23 0.5408055864317944 2306 24-25 0.5455172996863524 2306 26-27 0.525635660732064 2306 28-29 0.5454500645628428 2306 30-31 0.5788939714550949 2306 32-33 0.6180584611064717 2306 34-35 0.6372776595837806 2306 36-37 0.6374934817860591 2306 38-39 0.5976181741311422 2306 40-41 0.6234351180913436 2306 42-43 0.577062634067012 2306 44-45 0.6439418931952687 2306 46-47 0.6299143322443257 2306 48-49 0.5743956943854016 2306 50-51 0.5447828472872303 2306 52-53 0.6094850285637392 2306 54-55 0.630708157581239 2306 56-57 0.626747231691418 2306 58-59 0.6937095038129755 2306 60-61 0.6867944605632132 2306 62-63 0.6801890144175573 2306 64-65 0.7086302251437999 2306 66-67 0.7286374823286437 2306 68-69 0.7402663525271862 2306 70-71 0.7314812433436586 2306 72-73 0.7842858752608848 2306 74-75 0.7758951587617204 2306 76-77 0.8391754729372138 2306 78-79 0.7883084260778546 2306 80-81 0.7999556198844004 2306 82-83 0.803963867836643 2306 84-85 0.7916442520693181 2306 86-87 0.7863423855009373 2306 88-89 0.8294180710859607 2306 90-91 0.8496308047993324 2306 92-93 0.8350348047497747 2306 94-95 0.8275353495917912 2306 96-97 0.8150251316752488 2306 98-99 0.7284582192209399 2306 100-101 0.8688072082025045 2307 1 0.14998514297011667 2307 2 0.1897661776414381 2307 3 0.19591635015193276 2307 4 0.27874935919145827 2307 5 0.2498255405162837 2307 6 0.30261369200698596 2307 7 0.2926093465937143 2307 8 0.27724198366991004 2307 9 0.2997701340277885 2307 10-11 0.3026834387611572 2307 12-13 0.28432396939619764 2307 14-15 0.5163031285423116 2307 16-17 0.5196777261513219 2307 18-19 0.49617087860407594 2307 20-21 0.5507133687443826 2307 22-23 0.5453002731632566 2307 24-25 0.5430128973047417 2307 26-27 0.5550137317554871 2307 28-29 0.5795585939133616 2307 30-31 0.5805486658858072 2307 32-33 0.6716269125056868 2307 34-35 0.5669536829362656 2307 36-37 0.6298733008823447 2307 38-39 0.6030945865961996 2307 40-41 0.5962029621371983 2307 42-43 0.6005936410728978 2307 44-45 0.6164897076033071 2307 46-47 0.6374733341755743 2307 48-49 0.5884901132173681 2307 50-51 0.544370336437801 2307 52-53 0.6162766100509103 2307 54-55 0.618128544418397 2307 56-57 0.5662337147162262 2307 58-59 0.6245354056303825 2307 60-61 0.6044114286764497 2307 62-63 0.6230607339187628 2307 64-65 0.676862954248314 2307 66-67 0.6336165204807074 2307 68-69 0.64181650604705 2307 70-71 0.6402358469718976 2307 72-73 0.7171242432778087 2307 74-75 0.6510331692215416 2307 76-77 0.7100853366333268 2307 78-79 0.7443350557461557 2307 80-81 0.7312022613019238 2307 82-83 0.7698166991350917 2307 84-85 0.7919644578023934 2307 86-87 0.8141763135702078 2307 88-89 0.8618973078013639 2307 90-91 0.8642139398674828 2307 92-93 0.784675566116718 2307 94-95 0.7851948496128998 2307 96-97 0.8025678764573243 2307 98-99 0.8430360592669075 2307 100-101 0.959703061556155 2201 1 0.18748397142197604 2201 2 0.19490855861941725 2201 3 0.23546800301755155 2201 4 0.2577112629320908 2201 5 0.26772787500948425 2201 6 0.2791867633526053 2201 7 0.23534867054171826 2201 8 0.24991266216773056 2201 9 0.2676238850411323 2201 10-11 0.2301948181407667 2201 12-13 0.2682419322243206 2201 14-15 0.4721024583651712 2201 16-17 0.48392836268075 2201 18-19 0.5098287141197559 2201 20-21 0.5528392771222315 2201 22-23 0.517699584053716 2201 24-25 0.5203130086756929 2201 26-27 0.5455884569189706 2201 28-29 0.5862501626038963 2201 30-31 0.5535749837121031 2201 32-33 0.6251417586357917 2201 34-35 0.6161949184661708 2201 36-37 0.6222309370260888 2201 38-39 0.6187809477493786 2201 40-41 0.5614529444760237 2201 42-43 0.5665130124795112 2201 44-45 0.6335328364300707 2201 46-47 0.6102491822802634 2201 48-49 0.5844752798933612 2201 50-51 0.5301541450655165 2201 52-53 0.6053455147193034 2201 54-55 0.6812353512374045 2201 56-57 0.6909526487073308 2201 58-59 0.7151774168260161 2201 60-61 0.7093704148752096 2201 62-63 0.6791021086365703 2201 64-65 0.6399547080223371 2201 66-67 0.6633881739036056 2201 68-69 0.6761773914719384 2201 70-71 0.7002542464931238 2201 72-73 0.7609993661437713 2201 74-75 0.7172805694166584 2201 76-77 0.7989402854168475 2201 78-79 0.7961783712325499 2201 80-81 0.7763271762444788 2201 82-83 0.7385145277157648 2201 84-85 0.7195278825338391 2201 86-87 0.7369768028856427 2201 88-89 0.7999168524610596 2201 90-91 0.8146589311438461 2201 92-93 0.7848939971043976 2201 94-95 0.8126443984577989 2201 96-97 0.7463328151620203 2201 98-99 0.7757067068048968 2201 100-101 0.7506516595120729 1101 1 0.11454874011520388 1101 2 0.14620830680462404 1101 3 0.1530323377750662 1101 4 0.20383478349100415 1101 5 0.26272149223078856 1101 6 0.2691384127356571 1101 7 0.23301233267944355 1101 8 0.26595911543281403 1101 9 0.26718342280830143 1101 10-11 0.268852935069539 1101 12-13 0.2692215702667653 1101 14-15 0.4832898028262136 1101 16-17 0.48738011345790966 1101 18-19 0.45174383520607364 1101 20-21 0.474085927425989 1101 22-23 0.4954753935166991 1101 24-25 0.5008000034241746 1101 26-27 0.501290574230012 1101 28-29 0.5617838025281543 1101 30-31 0.5126016235578987 1101 32-33 0.5680787458865524 1101 34-35 0.5269981307559206 1101 36-37 0.5813551249991136 1101 38-39 0.5852618029096419 1101 40-41 0.5987032760464146 1101 42-43 0.5398432534867013 1101 44-45 0.5789532412761673 1101 46-47 0.5654075008318173 1101 48-49 0.5398781097602594 1101 50-51 0.4962223145405247 1101 52-53 0.518647800476657 1101 54-55 0.5351022739921802 1101 56-57 0.573548640959423 1101 58-59 0.5948650297289433 1101 60-61 0.6080075770210982 1101 62-63 0.5841919764512866 1101 64-65 0.6294031162901987 1101 66-67 0.546356488438235 1101 68-69 0.5625601235498507 1101 70-71 0.5587114865376606 1101 72-73 0.6755112719878795 1101 74-75 0.6318055282957076 1101 76-77 0.6661452732301285 1101 78-79 0.6444251077795684 1101 80-81 0.6783693546406155 1101 82-83 0.6813737794816177 1101 84-85 0.6952043677244717 1101 86-87 0.7211790565542842 1101 88-89 0.7484309042862733 1101 90-91 0.7657233362168157 1101 92-93 0.692296505307219 1101 94-95 0.7176603767711143 1101 96-97 0.7378144667128126 1101 98-99 0.7673459659830115 1101 100-101 0.7557766073582073 2304 1 0.1469127573042215 2304 2 0.1649452412856931 2304 3 0.16556631150474743 2304 4 0.24077805378532702 2304 5 0.2298531764353342 2304 6 0.21510300974298957 2304 7 0.20964787197268464 2304 8 0.24079293149735292 2304 9 0.24211337945715172 2304 10-11 0.280293360359785 2304 12-13 0.253424326837731 2304 14-15 0.45422164307328927 2304 16-17 0.4560805388563338 2304 18-19 0.4542589729534896 2304 20-21 0.46145013392136036 2304 22-23 0.43589939918507525 2304 24-25 0.4628203822532342 2304 26-27 0.47115016300252677 2304 28-29 0.5083997148089949 2304 30-31 0.47745679743578506 2304 32-33 0.5753115670109707 2304 34-35 0.5420969689557822 2304 36-37 0.579496711278189 2304 38-39 0.5676884940238693 2304 40-41 0.5579115765891842 2304 42-43 0.5354476275452456 2304 44-45 0.5669670052003184 2304 46-47 0.5917742251017017 2304 48-49 0.5194720341080341 2304 50-51 0.5868538472999916 2304 52-53 0.6064640096980369 2304 54-55 0.5869725193602591 2304 56-57 0.5410856773954862 2304 58-59 0.5798027006117294 2304 60-61 0.5853560575671679 2304 62-63 0.5829231168057163 2304 64-65 0.580605346859933 2304 66-67 0.5325316481992743 2304 68-69 0.5632998043252542 2304 70-71 0.5322596082129678 2304 72-73 0.6240426603336431 2304 74-75 0.5856440188750156 2304 76-77 0.6733162655470011 2304 78-79 0.712067107828247 2304 80-81 0.6994008511614354 2304 82-83 0.6758663505593887 2304 84-85 0.7349076570575974 2304 86-87 0.6921166097966918 2304 88-89 0.7045359666086242 2304 90-91 0.7201889379000193 2304 92-93 0.6504560824509085 2304 94-95 0.6555673516285268 2304 96-97 0.7513369914646191 2304 98-99 0.7320776850740174 2304 100-101 0.8100494208022084 2202 1 0.10958155244711065 2202 2 0.15017867580888122 2202 3 0.17143183367928572 2202 4 0.22136809530805834 2202 5 0.25473157414635494 2202 6 0.2537856225347852 2202 7 0.21410720411965656 2202 8 0.22891760437498476 2202 9 0.24799059080437047 2202 10-11 0.27392232508291414 2202 12-13 0.22986183338016986 2202 14-15 0.38084094242078237 2202 16-17 0.4112721017161718 2202 18-19 0.41140280211710234 2202 20-21 0.4430559122109017 2202 22-23 0.4091941756950632 2202 24-25 0.4366447480720055 2202 26-27 0.40120773653963226 2202 28-29 0.49384782883949896 2202 30-31 0.4773010871876906 2202 32-33 0.5065719913104942 2202 34-35 0.5060328180782179 2202 36-37 0.5075618042681711 2202 38-39 0.5265673802376227 2202 40-41 0.5405117196507021 2202 42-43 0.5029853992748912 2202 44-45 0.542021002199526 2202 46-47 0.5310020418433865 2202 48-49 0.5210867101260348 2202 50-51 0.540415991827011 2202 52-53 0.5904513260733495 2202 54-55 0.5829363527110374 2202 56-57 0.5781837094273072 2202 58-59 0.5671282353477665 2202 60-61 0.5466357602783631 2202 62-63 0.570751983828302 2202 64-65 0.5956065681167786 2202 66-67 0.540113385175438 2202 68-69 0.5311377435415494 2202 70-71 0.5635732444180022 2202 72-73 0.658121658205161 2202 74-75 0.6052744142133051 2202 76-77 0.6420690133749076 2202 78-79 0.628533488371879 2202 80-81 0.635773107695158 2202 82-83 0.6307401249409139 2202 84-85 0.6010434259260222 2202 86-87 0.6026065082579137 2202 88-89 0.6225088547066022 2202 90-91 0.6909834527121177 2202 92-93 0.6211827302753292 2202 94-95 0.6025110691343727 2202 96-97 0.6847974227427471 2202 98-99 0.610172437158873 2202 100-101 0.6471336700781691 1102 1 0.033071019058894535 1102 2 0.08966438773001784 1102 3 0.10163988796984569 1102 4 0.12394981536581895 1102 5 0.07706928287034742 1102 6 0.11380147760895198 1102 7 0.10233372260235996 1102 8 0.11106400681945416 1102 9 0.10437245416760277 1102 10-11 0.11357296897751468 1102 12-13 0.1095937780877847 1102 14-15 0.15323640891497092 1102 16-17 0.17856902935997 1102 18-19 0.1944873980849522 1102 20-21 0.2341803592978522 1102 22-23 0.19105290001496655 1102 24-25 0.21993055974072462 1102 26-27 0.2176966434324541 1102 28-29 0.20735062416747496 1102 30-31 0.26677684612958785 1102 32-33 0.3034328172988836 1102 34-35 0.17767216305457367 1102 36-37 0.2130685459415389 1102 38-39 0.24513162486748286 1102 40-41 0.2810778416258799 1102 42-43 0.25654181437293033 1102 44-45 0.3061936308532083 1102 46-47 0.2524458554243054 1102 48-49 0.2876764971061547 1102 50-51 0.2611805853556177 1102 52-53 0.28256009449561503 1102 54-55 0.32890530899851456 1102 56-57 0.33313977039964726 1102 58-59 0.3440204448002717 1102 60-61 0.32333470334600634 1102 62-63 0.30278925196417106 1102 64-65 0.38853740471150644 1102 66-67 0.36324456631313495 1102 68-69 0.3834920913818394 1102 70-71 0.3376787721327972 1102 72-73 0.46321821517126693 1102 74-75 0.4002067796901869 1102 76-77 0.4460086944004331 1102 78-79 0.3714471413766134 1102 80-81 0.3837260390326733 1102 82-83 0.3752773331846768 1102 84-85 0.3459053147270623 1102 86-87 0.33361040886750715 1102 88-89 0.41994218390193794 1102 90-91 0.4217177952819071 1102 92-93 0.3887263698424519 1102 94-95 0.4397178754912119 1102 96-97 0.37597797239983066 1102 98-99 0.4013866352906774 1102 100-101 0.47216419327384074 2305 1 0.13431558389982712 2305 2 0.1642949221321075 2305 3 0.1384497567631371 2305 4 0.13890837776303044 2305 5 0.16961223703025752 2305 6 0.19338542307210815 2305 7 0.16111866883035475 2305 8 0.1992730565379901 2305 9 0.18830155927476966 2305 10-11 0.1922179735892513 2305 12-13 0.20046520798997847 2305 14-15 0.322497031853878 2305 16-17 0.3385580253184415 2305 18-19 0.3520417417137409 2305 20-21 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-1.126309725911419 1213 76-77 -1.0346339922533758 1213 78-79 -1.1554307656474734 1213 80-81 -1.1539255140016067 1213 82-83 -1.1245371188351783 1213 84-85 -1.132063346389927 1213 86-87 -1.1830331636796458 1213 88-89 -1.2365828172329643 1213 90-91 -1.3678233467359746 1213 92-93 -1.2407656904471978 1213 94-95 -1.2085579779129816 1213 96-97 -1.2916643332561506 1213 98-99 -1.3896509320301789 1213 100-101 -1.3843279575589733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12639.0 3 925.0 4 664.0 5 547.0 6 542.0 7 1582.0 8 2713.0 9 4615.0 10 7881.0 11 10543.0 12 10260.0 13 9108.0 14 8515.0 15 8509.0 16 9138.0 17 10393.0 18 11785.0 19 13214.0 20 14598.0 21 16611.0 22 18771.0 23 21585.0 24 25064.0 25 28946.0 26 33298.0 27 40432.0 28 48139.0 29 60199.0 30 75649.0 31 98177.0 32 132127.0 33 182511.0 34 256929.0 35 388481.0 36 797261.0 37 3597539.0 38 3548157.0 39 1687.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.85973603130178 13.524403854328915 22.49985148758879 41.11600862678051 2 26.082289776844657 23.776722282876726 30.735423637298243 19.405564302980373 3 23.89989845265094 26.119016610500722 30.769036336510347 19.212048600337987 4 25.380761496666192 29.764377381618708 22.10240098231817 22.75246013939693 5 28.318354847591916 33.09934255335868 21.602463776390195 16.97983882265921 6 19.16622644386759 37.500631898754655 22.157413983835188 21.175727673542568 7 18.335599229121506 15.623159255876242 43.64677680931752 22.394464705684726 8 22.40342229519086 21.35363636239186 28.411941176774114 27.831000165643164 9 24.576008655891954 22.636712185167156 26.856238312581386 25.931040846359497 10-11 24.326454015792468 30.905604024108808 21.996941250195405 22.771000709903323 12-13 22.833173393510297 25.009560589816793 28.11829482037147 24.038971196301443 14-15 23.28095789232419 26.53815906550513 27.89332138456085 22.287561657609828 16-17 24.07596440094601 26.512762761971736 26.952472148332063 22.4588006887502 18-19 23.83457655316181 26.780363080624518 26.86778080190747 22.5172795643062 20-21 23.873792414671136 27.13862912541267 26.658998906216524 22.32857955369967 22-23 23.981310438812297 26.96407058461435 26.803753022022935 22.25086595455042 24-25 23.552045774283215 27.343466109420877 26.852418541627443 22.252069574668468 26-27 23.951261273816872 27.461330848951054 26.430444933441326 22.156962943790745 28-29 24.020301698658407 26.775957435633575 26.860793687811967 22.342947177896047 30-31 23.609838605145434 27.067774002659682 26.918314919124082 22.4040724730708 32-33 23.784040598524562 27.164528104614817 26.81557890485799 22.23585239200263 34-35 24.079238066184917 26.75879548507308 26.883310068595467 22.278656380146536 36-37 23.645505503641214 26.989273556439763 27.062469688152984 22.30275125176604 38-39 24.081129942026866 27.072560064670686 26.785422718043932 22.060887275258512 40-41 24.17634243669231 26.612877731228984 26.834560560709924 22.376219271368782 42-43 23.84818348841344 26.764790559539563 27.055922347998447 22.331103604048547 44-45 24.27958310370214 26.866056860326594 26.73943686416433 22.11492317180694 46-47 24.141498400051074 26.77721066756228 26.824971044580074 22.256319887806573 48-49 23.980839939466367 26.70679266492901 26.981310309277372 22.331057086327245 50-51 24.240864137964678 26.96834884914054 26.493286025320877 22.2975009875739 52-53 24.351819057743388 26.765047821709985 26.746435787560536 22.136697332986092 54-55 23.954361231058378 26.913295322440074 26.917486887273917 22.214856559227627 56-57 24.330458446607047 26.982223015336388 26.803851312297073 21.883467225759496 58-59 24.626723557720158 26.9332811609651 26.657569856027436 21.782425425287308 60-61 24.10614845665998 27.068832784157575 26.589443450116057 22.235575309066384 62-63 24.80898027295799 27.105692685367462 26.314222567218774 21.771104474455775 64-65 24.79839733279234 27.052635260068968 26.461636453916576 21.687330953222116 66-67 24.674545177507717 27.20136108745844 26.50651251991999 21.61758121511385 68-69 25.147828584212057 27.23629433323366 26.217421862748935 21.398455219805356 70-71 25.25859297257827 27.27092230927407 26.05683207512938 21.413652643018278 72-73 25.223114836651416 27.347214957480652 25.969316084193135 21.460354121674794 74-75 25.575727202471487 27.410385123368837 25.785490155003192 21.228397519156484 76-77 25.901970000939674 27.298632634332705 25.831602472370918 20.967794892356707 78-79 25.887751567878837 27.29821943047932 25.840001614498536 20.974027387143312 80-81 26.33276715463253 27.465723343379437 25.337914810009494 20.86359469197854 82-83 26.62705303013985 27.38022155317678 25.35365600553891 20.639069411144455 84-85 26.588211599573995 27.36263064308692 25.454594675476038 20.59456308186304 86-87 27.003063230131858 27.494748345852045 25.133480146353804 20.368708277662297 88-89 27.346561955580643 27.379635266682534 25.069933200337896 20.20386957739892 90-91 27.431633311283626 27.373131974685833 25.09112161235941 20.104113101671132 92-93 27.94042889648363 27.541732207249183 24.615960580278838 19.90187831598835 94-95 28.166724451270937 27.47527338507127 24.721291092015278 19.63671107164252 96-97 28.102545354427725 27.692914373574734 24.769247832141406 19.435292439856138 98-99 28.736404923005242 27.66951963490825 24.44450950616713 19.149565935919373 100-101 28.95679346389341 27.588291933230973 24.304224240730136 19.150690362145482 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10827.0 1 5928.5 2 814.0 3 501.5 4 393.5 5 329.5 6 295.0 7 328.0 8 320.5 9 311.0 10 326.0 11 325.0 12 352.5 13 389.0 14 426.0 15 495.5 16 536.5 17 592.0 18 661.0 19 772.0 20 922.0 21 1080.0 22 1345.0 23 1819.5 24 2553.5 25 3602.5 26 5250.5 27 7739.5 28 11517.5 29 16756.5 30 23609.5 31 32041.0 32 42233.0 33 54026.0 34 68177.0 35 85948.5 36 109403.0 37 139402.0 38 175142.0 39 219232.0 40 274683.5 41 338468.5 42 405256.0 43 474061.5 44 539400.5 45 592560.5 46 627588.0 47 640805.5 48 633239.5 49 608918.5 50 565424.5 51 507779.0 52 441857.5 53 374583.0 54 310210.0 55 253519.5 56 207952.0 57 171214.0 58 141123.5 59 113554.0 60 88650.5 61 62807.0 62 39851.0 63 25612.5 64 16621.0 65 10168.0 66 6695.5 67 4192.5 68 2749.5 69 2133.0 70 1866.0 71 1418.5 72 837.5 73 618.5 74 570.5 75 461.0 76 349.5 77 182.5 78 92.0 79 67.5 80 57.0 81 44.5 82 34.0 83 24.0 84 26.0 85 22.5 86 17.0 87 11.5 88 8.5 89 9.0 90 7.0 91 3.5 92 1.5 93 3.0 94 2.0 95 0.0 96 0.0 97 0.5 98 0.5 99 1.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1638111013410049 2 0.14975182271133977 3 0.1541157723233899 4 0.14541941972299122 5 0.171823943761203 6 0.15291700062273034 7 0.153453293225657 8 0.14108701673464263 9 0.1691424807465698 10-11 0.14059804406726834 12-13 0.1305188977946176 14-15 0.1903681007271076 16-17 0.19895404014455084 18-19 0.18275484887379606 20-21 0.21717747310282284 22-23 0.20185107175447808 24-25 0.19233976470845557 26-27 0.17698707450702617 28-29 0.1946268949268192 30-31 0.14184939347409717 32-33 0.16917928514088829 34-35 0.17317519080975347 36-37 0.17609325350214844 38-39 0.17577778726513277 40-41 0.21083660173880783 42-43 0.1963409281479377 44-45 0.18042039871988005 46-47 0.1846108419015716 48-49 0.22561093717237518 50-51 0.2030025235195853 52-53 0.16694999039931085 54-55 0.15219668604821124 56-57 0.14841109120402315 58-59 0.16366388376373092 60-61 0.158264153340146 62-63 0.14708087523794042 64-65 0.16887959221572338 66-67 0.1729017867376732 68-69 0.18480537941439792 70-71 0.16521492609572466 72-73 0.17439499359288071 74-75 0.2075347217913771 76-77 0.23558492803268735 78-79 0.21787675659487427 80-81 0.1927025508810236 82-83 0.16240727658628518 84-85 0.17202899681526318 86-87 0.16135572246289961 88-89 0.16482059329945506 90-91 0.1765401640045873 92-93 0.16087200756614223 94-95 0.15459948722014727 96-97 0.16253872085170837 98-99 0.15223349044252973 100-101 0.15933148077538237 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9509734.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.774642115271504 #Duplication Level Percentage of deduplicated Percentage of total 1 71.699918269699 32.82038098489688 2 13.710422686616546 12.551793834579433 3 5.425431528431464 7.45041559604582 4 2.6082852857580376 4.775733019604115 5 1.5858676208272435 3.629626139278208 6 0.9938391280379865 2.7295578243655396 7 0.6825519781913026 2.1870500768744234 8 0.5401021523981948 1.9778386185372157 9 0.39924586467701617 1.644780291443328 >10 2.122405775965969 17.664469591629427 >50 0.14807515794685683 4.620999018779078 >100 0.07927919292594177 6.217244373156064 >500 0.003608548541603316 1.0881162042444272 >1k 9.668099830561671E-4 0.6419944265660695 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.4701286071723984E-4 1.0515541233855753E-5 0.0 2 5.257770616927877E-4 1.0515541233855753E-5 0.0 3 6.729946389667682E-4 1.0515541233855753E-5 0.0 4 0.0017560953860539107 1.0515541233855753E-5 0.0 5 0.001871766339626324 1.0515541233855753E-5 0.0 6 0.0023134190714482654 3.154662370156726E-5 0.0 7 0.0028391961331410533 3.154662370156726E-5 0.0 8 0.004490136106856406 3.154662370156726E-5 0.0 9 0.004931788838678348 3.154662370156726E-5 0.0 10-11 0.005709938889983674 3.154662370156726E-5 0.0 12-13 0.006382933528950442 5.7835476786206644E-5 5.257770616927876E-6 14-15 0.007481807587888368 6.309324740313452E-5 1.0515541233855753E-5 16-17 0.008664805976697141 6.309324740313452E-5 1.0515541233855753E-5 18-19 0.011446166633051987 7.886655925391815E-5 1.0515541233855753E-5 20-21 0.013933092134858872 9.463987110470177E-5 1.0515541233855753E-5 22-23 0.016730226103064502 9.463987110470177E-5 1.0515541233855753E-5 24-25 0.020810256101800532 9.989764172162965E-5 1.0515541233855753E-5 26-27 0.026851434540650664 1.1567095357241327E-4 1.0515541233855753E-5 28-29 0.03998008777111957 1.2092872418934116E-4 1.0515541233855753E-5 30-31 0.06270417237748185 1.2618649480626904E-4 1.0515541233855753E-5 32-33 0.09536018567922089 1.2618649480626904E-4 1.0515541233855753E-5 34-35 0.13703853335960817 1.2618649480626904E-4 1.0515541233855753E-5 36-37 0.19455854390879912 1.367020360401248E-4 1.0515541233855753E-5 38-39 0.2718267408951712 1.4721757727398054E-4 1.0515541233855753E-5 40-41 0.37128798765559584 1.6824865974169204E-4 1.0515541233855753E-5 42-43 0.5015229658368994 1.6824865974169204E-4 1.0515541233855753E-5 44-45 0.6589721647314215 1.787642009755478E-4 1.0515541233855753E-5 46-47 0.842063510924701 1.9453751282633142E-4 2.1031082467711505E-5 48-49 1.0604502712694173 2.1556859529404292E-4 2.1031082467711505E-5 50-51 1.3096160208056293 2.208263659109708E-4 2.1031082467711505E-5 52-53 1.6022319867201333 2.208263659109708E-4 2.1031082467711505E-5 54-55 1.9425832520657254 2.208263659109708E-4 2.1031082467711505E-5 56-57 2.330443732705878 2.208263659109708E-4 3.154662370156726E-5 58-59 2.7788053798350196 2.418574483786823E-4 4.206216493542301E-5 60-61 3.2921635873306236 2.418574483786823E-4 4.206216493542301E-5 62-63 3.8831107158202323 2.523729896125381E-4 4.206216493542301E-5 64-65 4.5557635996969 2.523729896125381E-4 4.206216493542301E-5 66-67 5.30802964625509 2.523729896125381E-4 4.206216493542301E-5 68-69 6.146233953546966 2.523729896125381E-4 4.206216493542301E-5 70-71 7.077931937949053 2.523729896125381E-4 4.206216493542301E-5 72-73 8.106141559795468 2.523729896125381E-4 4.206216493542301E-5 74-75 9.216330341101024 2.523729896125381E-4 4.206216493542301E-5 76-77 10.398256144703943 2.523729896125381E-4 4.206216493542301E-5 78-79 11.661940281400089 2.523729896125381E-4 4.206216493542301E-5 80-81 12.99777154650172 2.523729896125381E-4 4.206216493542301E-5 82-83 14.405329318359485 2.523729896125381E-4 4.206216493542301E-5 84-85 15.890044874020662 2.523729896125381E-4 4.206216493542301E-5 86-87 17.428026903801936 2.57630760229466E-4 4.206216493542301E-5 88-89 19.020132424313864 2.6288853084639383E-4 4.206216493542301E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGCGC 905 0.0 18.638052 42-43 GGCGCCC 360 0.0 18.478312 44-45 TCGGGGG 1570 0.0 16.188995 38-39 GGGGGGC 1240 0.0 14.559063 40-41 GGGGGCG 1340 0.0 12.763483 40-41 GGGCCCG 315 1.5952537E-9 12.067469 44-45 GTAGGGG 1175 0.0 11.524406 18-19 GGGGCCC 505 0.0 11.29085 44-45 CGGGAAT 2735 0.0 10.938189 1 GGGCGCC 1765 0.0 10.902624 42-43 CGAAAAT 5100 0.0 10.8006525 1 GGGCGGG 1320 0.0 10.796453 12-13 GCGCCCG 510 0.0 10.714315 44-45 CGGGGGG 1730 0.0 10.572566 38-39 CGAAAAC 5635 0.0 10.196559 1 CGGAAAT 4510 0.0 10.107805 1 GAGCGGC 2425 0.0 9.990193 9 CAAAAAT 7670 0.0 9.967648 1 CCGAAAT 3065 0.0 9.915433 1 GAGGGGC 1265 0.0 9.387824 9 >>END_MODULE