FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n02 tsrexp_lib#10.3420000000d08b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n02 tsrexp_lib#10.3420000000d08b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9509734
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCGC9050.018.63805242-43
GGCGCCC3600.018.47831244-45
TCGGGGG15700.016.18899538-39
GGGGGGC12400.014.55906340-41
GGGGGCG13400.012.76348340-41
GGGCCCG3151.5952537E-912.06746944-45
GTAGGGG11750.011.52440618-19
GGGGCCC5050.011.2908544-45
CGGGAAT27350.010.9381891
GGGCGCC17650.010.90262442-43
CGAAAAT51000.010.80065251
GGGCGGG13200.010.79645312-13
GCGCCCG5100.010.71431544-45
CGGGGGG17300.010.57256638-39
CGAAAAC56350.010.1965591
CGGAAAT45100.010.1078051
GAGCGGC24250.09.9901939
CAAAAAT76700.09.9676481
CCGAAAT30650.09.9154331
GAGGGGC12650.09.3878249
Produced by FastQC (version 0.11.2)