##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l02n02 tsrexp_lib#1.3420000000d055.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9549549 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49930651175254 33.0 33.0 33.0 33.0 33.0 2 32.42183196295448 33.0 33.0 33.0 33.0 33.0 3 32.39911905787383 33.0 33.0 33.0 33.0 33.0 4 36.25686312515911 37.0 37.0 37.0 37.0 37.0 5 36.24710433969185 37.0 37.0 37.0 37.0 37.0 6 36.29930460590338 37.0 37.0 37.0 37.0 37.0 7 36.27525436017973 37.0 37.0 37.0 37.0 37.0 8 36.32183938738887 37.0 37.0 37.0 37.0 37.0 9 36.2506821002751 37.0 37.0 37.0 37.0 37.0 10-11 36.23976053738245 37.0 37.0 37.0 37.0 37.0 12-13 36.24112620397047 37.0 37.0 37.0 37.0 37.0 14-15 38.57614553315554 40.0 40.0 40.0 37.0 40.0 16-17 38.548915084890396 40.0 40.0 40.0 37.0 40.0 18-19 38.557794090590036 40.0 40.0 40.0 37.0 40.0 20-21 38.511217178947405 40.0 40.0 40.0 37.0 40.0 22-23 38.57634302939333 40.0 40.0 40.0 37.0 40.0 24-25 38.51280887715221 40.0 40.0 40.0 37.0 40.0 26-27 38.50236377655112 40.0 40.0 40.0 37.0 40.0 28-29 38.4325947748946 40.0 40.0 40.0 37.0 40.0 30-31 38.40357717416812 40.0 40.0 40.0 37.0 40.0 32-33 38.325875860734364 40.0 40.0 40.0 37.0 40.0 34-35 38.315633754012886 40.0 40.0 40.0 37.0 40.0 36-37 38.27953786089793 40.0 40.0 40.0 37.0 40.0 38-39 38.26518660724187 40.0 38.5 40.0 37.0 40.0 40-41 38.22641854604861 40.0 37.0 40.0 37.0 40.0 42-43 38.15030934968762 40.0 37.0 40.0 37.0 40.0 44-45 38.11507700520726 40.0 37.0 40.0 37.0 40.0 46-47 37.99729714984446 40.0 37.0 40.0 37.0 40.0 48-49 37.90608676912386 40.0 37.0 40.0 37.0 40.0 50-51 36.51811404915562 38.5 37.0 38.5 33.0 40.0 52-53 36.84538620619675 38.5 37.0 40.0 33.0 40.0 54-55 37.49958872403293 40.0 37.0 40.0 33.0 40.0 56-57 37.47629573920192 40.0 37.0 40.0 33.0 40.0 58-59 37.269933114118786 38.5 37.0 40.0 33.0 40.0 60-61 37.12496265530446 37.0 37.0 40.0 33.0 40.0 62-63 36.90528139077563 37.0 37.0 40.0 33.0 40.0 64-65 36.62600003413773 37.0 37.0 40.0 33.0 40.0 66-67 36.37224820774259 37.0 37.0 40.0 33.0 40.0 68-69 36.08287124344825 37.0 37.0 37.0 33.0 40.0 70-71 35.822332394964405 37.0 37.0 37.0 33.0 40.0 72-73 35.53833976871578 37.0 37.0 37.0 33.0 38.5 74-75 35.38868139217884 37.0 37.0 37.0 33.0 37.0 76-77 35.181426944874566 37.0 37.0 37.0 33.0 37.0 78-79 35.0852722992468 37.0 37.0 37.0 33.0 37.0 80-81 34.9911027211861 37.0 37.0 37.0 33.0 37.0 82-83 34.91459690923624 37.0 37.0 37.0 33.0 37.0 84-85 34.863581882243864 37.0 35.0 37.0 33.0 37.0 86-87 34.80820853424596 37.0 33.0 37.0 33.0 37.0 88-89 34.730854567058614 37.0 33.0 37.0 33.0 37.0 90-91 34.674702857695166 37.0 33.0 37.0 33.0 37.0 92-93 34.64860005430623 37.0 33.0 37.0 33.0 37.0 94-95 34.59145882177263 37.0 33.0 37.0 33.0 37.0 96-97 34.52153122623906 37.0 33.0 37.0 33.0 37.0 98-99 34.46468660457159 37.0 33.0 37.0 33.0 37.0 100-101 32.68218410105022 35.0 30.0 37.0 27.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16008845804807237 2306 2 0.19285810176111085 2306 3 0.16813060086951737 2306 4 0.22518317406125732 2306 5 0.2199781440328934 2306 6 0.2996390624404057 2306 7 0.23130558595446615 2306 8 0.2566532546736795 2306 9 0.20427752448078706 2306 10-11 0.2516552428031389 2306 12-13 0.23730707550161867 2306 14-15 0.4322124149638853 2306 16-17 0.4849535019777207 2306 18-19 0.4557678013011852 2306 20-21 0.5191051051082596 2306 22-23 0.5728180083854255 2306 24-25 0.5198302402938921 2306 26-27 0.5040811261978462 2306 28-29 0.5139430547713175 2306 30-31 0.5212469786051699 2306 32-33 0.6079832083191334 2306 34-35 0.5758601014990816 2306 36-37 0.5725343905575571 2306 38-39 0.5653799515624698 2306 40-41 0.6129854824900676 2306 42-43 0.5528447483491874 2306 44-45 0.6127416926681164 2306 46-47 0.5292088586082713 2306 48-49 0.528678362816656 2306 50-51 0.47124544828432136 2306 52-53 0.5748295864410764 2306 54-55 0.591475512360816 2306 56-57 0.6072692934933954 2306 58-59 0.619037154422557 2306 60-61 0.6048247674420395 2306 62-63 0.5711711142190339 2306 64-65 0.5698331026581585 2306 66-67 0.5580615284866752 2306 68-69 0.5995360153949179 2306 70-71 0.6163914428817776 2306 72-73 0.6811955202793598 2306 74-75 0.6482125393921478 2306 76-77 0.7309982563011417 2306 78-79 0.6921721649514083 2306 80-81 0.696204725096429 2306 82-83 0.7047320469798919 2306 84-85 0.6833154912323209 2306 86-87 0.6734950311648191 2306 88-89 0.7132081218526096 2306 90-91 0.6686243444436073 2306 92-93 0.7123621339295241 2306 94-95 0.7532806926725115 2306 96-97 0.7424386835391843 2306 98-99 0.6816794953290142 2306 100-101 0.7625383918124413 2307 1 0.12346575225288348 2307 2 0.19416387022103265 2307 3 0.22786297906865371 2307 4 0.2570234775663849 2307 5 0.24193651330016053 2307 6 0.27123515687681987 2307 7 0.2126549103964237 2307 8 0.2788552155532571 2307 9 0.2636044565119917 2307 10-11 0.2826850841674542 2307 12-13 0.2771465267144606 2307 14-15 0.505084948681052 2307 16-17 0.48142390354706066 2307 18-19 0.4891446855554378 2307 20-21 0.5477304092814492 2307 22-23 0.5448007975473459 2307 24-25 0.5205894586143316 2307 26-27 0.5034845216007398 2307 28-29 0.5858933009441003 2307 30-31 0.5258848353633852 2307 32-33 0.6461094963736755 2307 34-35 0.6024244171751931 2307 36-37 0.6263222831484825 2307 38-39 0.5709751188020533 2307 40-41 0.6324062324667921 2307 42-43 0.564819707961469 2307 44-45 0.5484763746881853 2307 46-47 0.6070534347092718 2307 48-49 0.5855352161778455 2307 50-51 0.5721009796814727 2307 52-53 0.6578226355015246 2307 54-55 0.6345947452167877 2307 56-57 0.6123090283312962 2307 58-59 0.6427343720222964 2307 60-61 0.60857012133566 2307 62-63 0.6069003049260075 2307 64-65 0.6424420027354998 2307 66-67 0.6257042064659473 2307 68-69 0.669552534884275 2307 70-71 0.632145412822787 2307 72-73 0.7236042733818238 2307 74-75 0.67812726461392 2307 76-77 0.7342838329244401 2307 78-79 0.739755860660793 2307 80-81 0.7346208380767862 2307 82-83 0.7604120891693711 2307 84-85 0.7898972475212673 2307 86-87 0.8115668868946173 2307 88-89 0.8305242058833642 2307 90-91 0.8289925255992401 2307 92-93 0.7521083340420844 2307 94-95 0.7031967287238743 2307 96-97 0.78180494551885 2307 98-99 0.8143370934843475 2307 100-101 0.8445192425661432 2201 1 0.12892436137102692 2201 2 0.171121470558667 2201 3 0.19760123655473194 2201 4 0.19465081894937697 2201 5 0.18233851576378157 2201 6 0.23835620367531618 2201 7 0.17417409438220233 2201 8 0.19024650058179304 2201 9 0.2216195436136701 2201 10-11 0.2069470917436007 2201 12-13 0.23126129608458967 2201 14-15 0.3876443474959075 2201 16-17 0.4107058808598012 2201 18-19 0.4551503908268302 2201 20-21 0.5281491518399761 2201 22-23 0.47767518292894806 2201 24-25 0.48807425828218953 2201 26-27 0.4927185346599856 2201 28-29 0.5456262528966391 2201 30-31 0.5323691237785368 2201 32-33 0.5869188068344542 2201 34-35 0.5613363195842496 2201 36-37 0.5816019538193942 2201 38-39 0.5560221780696253 2201 40-41 0.537601687979226 2201 42-43 0.5320331834161891 2201 44-45 0.5508286947117966 2201 46-47 0.5483348390072251 2201 48-49 0.5251514249473743 2201 50-51 0.4506496396160742 2201 52-53 0.560025547068534 2201 54-55 0.5650403371204646 2201 56-57 0.6121465812150717 2201 58-59 0.6064319557527895 2201 60-61 0.6279225588787654 2201 62-63 0.5774284521324731 2201 64-65 0.5507505669045756 2201 66-67 0.5307970683144845 2201 68-69 0.6087246143102902 2201 70-71 0.5846030071557706 2201 72-73 0.6916628812990382 2201 74-75 0.626765260812121 2201 76-77 0.6932841966344228 2201 78-79 0.6615825983195265 2201 80-81 0.6742979940019964 2201 82-83 0.6610912575087653 2201 84-85 0.6721691524694364 2201 86-87 0.736808616781623 2201 88-89 0.7330877822161384 2201 90-91 0.7080983523360445 2201 92-93 0.702716068540667 2201 94-95 0.6965059662710971 2201 96-97 0.7608183864897455 2201 98-99 0.7470583603960037 2201 100-101 0.7694784538644868 1101 1 0.14226100395884345 1101 2 0.14713048925017347 1101 3 0.15268492987265603 1101 4 0.17024068260018765 1101 5 0.2262631363674501 1101 6 0.21396073318046405 1101 7 0.19536315894442424 1101 8 0.21524298974507872 1101 9 0.23840034075595895 1101 10-11 0.22579245079027288 1101 12-13 0.23076701810658307 1101 14-15 0.4522400853313471 1101 16-17 0.4426932346026362 1101 18-19 0.42239652638301095 1101 20-21 0.44782006484005876 1101 22-23 0.41062495434736235 1101 24-25 0.44185673510442314 1101 26-27 0.4393724566875079 1101 28-29 0.4832045609093356 1101 30-31 0.4739374937581573 1101 32-33 0.5590240177742842 1101 34-35 0.5191286062803968 1101 36-37 0.5029718902177649 1101 38-39 0.5449360055540851 1101 40-41 0.4885319726624502 1101 42-43 0.4669348850878734 1101 44-45 0.4900937869925528 1101 46-47 0.479958704649988 1101 48-49 0.4908838297996496 1101 50-51 0.5258458886180861 1101 52-53 0.5369782800751111 1101 54-55 0.5658307457783422 1101 56-57 0.5565846579511131 1101 58-59 0.5890436862627624 1101 60-61 0.549053480538376 1101 62-63 0.5215338390625561 1101 64-65 0.5195767987932527 1101 66-67 0.5205352785942097 1101 68-69 0.5604066031605157 1101 70-71 0.5932965176348048 1101 72-73 0.6848505743752753 1101 74-75 0.6301682841516154 1101 76-77 0.6372797127516847 1101 78-79 0.5923707388696187 1101 80-81 0.6454508036578801 1101 82-83 0.6019991364945767 1101 84-85 0.637218997206574 1101 86-87 0.6528515069143666 1101 88-89 0.6664713921145378 1101 90-91 0.7005121666142031 1101 92-93 0.6391195959002047 1101 94-95 0.6164101085590019 1101 96-97 0.6419088529001371 1101 98-99 0.6126102134583462 1101 100-101 0.6963086074096552 2304 1 0.1476675392537672 2304 2 0.19149982460758963 2304 3 0.1810950801511737 2304 4 0.2520554846586478 2304 5 0.257586632344605 2304 6 0.2410990489036564 2304 7 0.22073381032496542 2304 8 0.2939747121568601 2304 9 0.24792075892935372 2304 10-11 0.27163224133281005 2304 12-13 0.2829970866532676 2304 14-15 0.47389491565103725 2304 16-17 0.46915311598579734 2304 18-19 0.4547467531928433 2304 20-21 0.5097876803321597 2304 22-23 0.4965906707642702 2304 24-25 0.4554274884955234 2304 26-27 0.5042558643568142 2304 28-29 0.5160504047692385 2304 30-31 0.46593962100926944 2304 32-33 0.592296460636625 2304 34-35 0.5377396069791729 2304 36-37 0.5794982717510564 2304 38-39 0.5928075433652324 2304 40-41 0.6296643372733399 2304 42-43 0.6032703586290324 2304 44-45 0.5681099979977731 2304 46-47 0.5622555693555 2304 48-49 0.5976559195333309 2304 50-51 0.6014363173496307 2304 52-53 0.5929794205899839 2304 54-55 0.5566527130406911 2304 56-57 0.5448058817331827 2304 58-59 0.5738194628548356 2304 60-61 0.5778349181299092 2304 62-63 0.577930458156807 2304 64-65 0.6028329110312498 2304 66-67 0.5624420737173139 2304 68-69 0.5392833728800497 2304 70-71 0.5711358588762394 2304 72-73 0.6477978510601758 2304 74-75 0.664556290851138 2304 76-77 0.7190058945429172 2304 78-79 0.6564123907599111 2304 80-81 0.7097817630357852 2304 82-83 0.7179290489255337 2304 84-85 0.6856013627823785 2304 86-87 0.7022072214643487 2304 88-89 0.7543895959725759 2304 90-91 0.7459273943538562 2304 92-93 0.6835006166117878 2304 94-95 0.6524274244000239 2304 96-97 0.7242063558174507 2304 98-99 0.7242549743153717 2304 100-101 0.8796220462628952 2202 1 0.14500146479220888 2202 2 0.15844318107171063 2202 3 0.1736032685504867 2202 4 0.2340227658148777 2202 5 0.23209206632022017 2202 6 0.23609813112879863 2202 7 0.21722132604843836 2202 8 0.23042208759554939 2202 9 0.22989337872461846 2202 10-11 0.2629080022942958 2202 12-13 0.24229033040144543 2202 14-15 0.3816961354044395 2202 16-17 0.447185362202994 2202 18-19 0.44817520258042975 2202 20-21 0.48815704590242603 2202 22-23 0.43752989595921576 2202 24-25 0.44809994472431214 2202 26-27 0.4844997726425504 2202 28-29 0.48198793808666807 2202 30-31 0.4743995974156192 2202 32-33 0.5435019933718266 2202 34-35 0.5578308422266716 2202 36-37 0.5364248758799306 2202 38-39 0.5849197434482178 2202 40-41 0.5588915043721698 2202 42-43 0.5166507702098357 2202 44-45 0.5519852145332322 2202 46-47 0.5877609958465513 2202 48-49 0.5785368923254808 2202 50-51 0.5237238817803203 2202 52-53 0.6101132460865202 2202 54-55 0.6038287090750245 2202 56-57 0.5627270125532391 2202 58-59 0.5366607818064324 2202 60-61 0.5563608052690938 2202 62-63 0.6062918864269875 2202 64-65 0.6117029289361042 2202 66-67 0.5776810487342061 2202 68-69 0.5943377869584054 2202 70-71 0.5762404221407564 2202 72-73 0.6469121996313305 2202 74-75 0.5917123800426296 2202 76-77 0.6096177041463235 2202 78-79 0.6069157616132372 2202 80-81 0.6557853939384088 2202 82-83 0.662168037184351 2202 84-85 0.6336511186888032 2202 86-87 0.6527505405161236 2202 88-89 0.661398498885923 2202 90-91 0.6908190443193902 2202 92-93 0.719675522569311 2202 94-95 0.67526584740164 2202 96-97 0.7147986008789857 2202 98-99 0.6816570706104912 2202 100-101 0.8199909137695514 1102 1 0.06537739821771282 1102 2 0.13892829983252142 1102 3 0.14529744191972327 1102 4 0.188641675451791 1102 5 0.1408379474019128 1102 6 0.15775888437505614 1102 7 0.15097457333541087 1102 8 0.18490523416922144 1102 9 0.1896525574598371 1102 10-11 0.1628102204228199 1102 12-13 0.19379138130811668 1102 14-15 0.24157584592544623 1102 16-17 0.23185378081630859 1102 18-19 0.2617549178287817 1102 20-21 0.28157840100071496 1102 22-23 0.21718640471920025 1102 24-25 0.25215702333282763 1102 26-27 0.3036834656496836 1102 28-29 0.265549505665831 1102 30-31 0.33501187022233125 1102 32-33 0.3631148108936699 1102 34-35 0.28454886262145607 1102 36-37 0.3155698283090089 1102 38-39 0.2450520587868894 1102 40-41 0.32330271988195847 1102 42-43 0.29910441775435714 1102 44-45 0.3432620556510173 1102 46-47 0.3408506250451211 1102 48-49 0.35455181129642455 1102 50-51 0.34545929877247517 1102 52-53 0.4008267635344751 1102 54-55 0.4074540500564865 1102 56-57 0.4061466691285176 1102 58-59 0.4441914072849542 1102 60-61 0.37308005620223383 1102 62-63 0.38255604088441686 1102 64-65 0.4200429382435331 1102 66-67 0.40104291039145323 1102 68-69 0.4245754796150578 1102 70-71 0.3633987210806495 1102 72-73 0.48888183621630077 1102 74-75 0.410262868421448 1102 76-77 0.49511888206608745 1102 78-79 0.45124771113202655 1102 80-81 0.4511139053140383 1102 82-83 0.43025182370130466 1102 84-85 0.42423469318733 1102 86-87 0.41964991407391494 1102 88-89 0.49248515610474186 1102 90-91 0.49878630899021204 1102 92-93 0.4655166751133848 1102 94-95 0.48594629472529505 1102 96-97 0.4709278142157771 1102 98-99 0.4780578686708381 1102 100-101 0.5492735455442528 2305 1 0.08925581718926878 2305 2 0.10553348053517908 2305 3 0.11607653597413758 2305 4 0.1564625200306793 2305 5 0.1493973273586775 2305 6 0.15530789328195738 2305 7 0.08237438094981542 2305 8 0.16954842775309942 2305 9 0.1591169651555404 2305 10-11 0.13611810543226 2305 12-13 0.14528741028171055 2305 14-15 0.2805153661839199 2305 16-17 0.2714089786529854 2305 18-19 0.29177782709783173 2305 20-21 0.31912437636954394 2305 22-23 0.2796669239784535 2305 24-25 0.3223523755034208 2305 26-27 0.33805227684710815 2305 28-29 0.30212639676299347 2305 30-31 0.3334344780218288 2305 32-33 0.3470249377406063 2305 34-35 0.3409668932942367 2305 36-37 0.3992355289990286 2305 38-39 0.41835953181816166 2305 40-41 0.375553098942909 2305 42-43 0.36572001608831073 2305 44-45 0.31934940638155496 2305 46-47 0.3388809754606257 2305 48-49 0.2760477867620921 2305 50-51 0.2144222469657464 2305 52-53 0.30369455507771903 2305 54-55 0.33448002821823053 2305 56-57 0.3697104497015147 2305 58-59 0.38551115687182147 2305 60-61 0.33397339754840516 2305 62-63 0.34393784718646003 2305 64-65 0.3583546398778452 2305 66-67 0.3747327369171245 2305 68-69 0.37424910427024827 2305 70-71 0.3829135197797413 2305 72-73 0.45811593005915086 2305 74-75 0.4232442677875383 2305 76-77 0.48225572535934447 2305 78-79 0.4204991293956084 2305 80-81 0.48403544211802085 2305 82-83 0.36989597107210415 2305 84-85 0.4247003352090246 2305 86-87 0.43156714591937373 2305 88-89 0.4425595239094591 2305 90-91 0.4742727706817149 2305 92-93 0.4725826506108888 2305 94-95 0.49085383287209794 2305 96-97 0.43107141273645055 2305 98-99 0.47614556776093053 2305 100-101 0.5277645919454983 2203 1 0.13785433371836575 2203 2 0.10980723142972693 2203 3 0.1302975393322754 2203 4 0.14354952392859843 2203 5 0.17877965482201574 2203 6 0.20235737322690994 2203 7 0.13994232192583667 2203 8 0.1464828826240705 2203 9 0.1373381483908318 2203 10-11 0.15818812299606577 2203 12-13 0.13966207059483793 2203 14-15 0.23184289678521708 2203 16-17 0.2703747304814996 2203 18-19 0.2708707168919062 2203 20-21 0.3010417091643589 2203 22-23 0.2839985131150584 2203 24-25 0.2692603273099934 2203 26-27 0.3119036053408024 2203 28-29 0.29135082210968477 2203 30-31 0.28436337444588133 2203 32-33 0.33127370451659743 2203 34-35 0.33573307459842994 2203 36-37 0.3902295224517047 2203 38-39 0.41022238048845594 2203 40-41 0.31730839669307187 2203 42-43 0.27616790642573363 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-1.1336618714579672 1213 72-73 -1.0315021343812276 1213 74-75 -1.1487281606119595 1213 76-77 -1.0751502121574674 1213 78-79 -1.177355564457045 1213 80-81 -1.1223004779152816 1213 82-83 -1.147502737350358 1213 84-85 -1.1301357907132754 1213 86-87 -1.1616682602496127 1213 88-89 -1.2471169114403509 1213 90-91 -1.3823448898215318 1213 92-93 -1.264821608693886 1213 94-95 -1.1984096013816057 1213 96-97 -1.234760175424391 1213 98-99 -1.3244652747341306 1213 100-101 -1.4059705066336434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12815.0 3 1020.0 4 698.0 5 511.0 6 772.0 7 3160.0 8 5382.0 9 8047.0 10 15006.0 11 23245.0 12 19396.0 13 14773.0 14 12556.0 15 11737.0 16 11389.0 17 12049.0 18 13084.0 19 14642.0 20 15950.0 21 17680.0 22 20102.0 23 22372.0 24 25593.0 25 28610.0 26 32741.0 27 38645.0 28 46466.0 29 57326.0 30 72089.0 31 93709.0 32 125768.0 33 174210.0 34 245802.0 35 372762.0 36 769939.0 37 3546468.0 38 3661197.0 39 1838.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.48583986771933 14.732052762903008 21.204692674424997 40.577414694952665 2 25.405610961244125 25.332743513926726 29.511515426860818 19.75013009796833 3 23.370427068448922 26.365838299814655 30.790750807796126 19.4729838239403 4 25.13487947732433 29.314187435438967 22.693656894463352 22.85727619277336 5 28.66251205305105 32.52884550023624 21.875123257919768 16.93351918879295 6 20.08502797767827 37.30475616335373 22.030376484151017 20.579839374816984 7 18.93137498491038 16.71532227387099 42.588131499876084 21.765171241342546 8 22.3921853412228 22.340024696083493 28.5475294740678 26.720260488625907 9 24.80803951490851 22.876329614824467 27.160887642066495 25.154743228200527 10-11 24.58918996973252 31.115453613195587 22.047373855872348 22.24798256119955 12-13 23.287999228266422 25.170337119651208 27.636471541799963 23.905192110282407 14-15 23.321892732114406 26.680992511062808 27.7977809647183 22.199333792104483 16-17 24.351434458696474 26.596213165865272 26.936505075182883 22.11584730025537 18-19 24.12762443884778 26.82475349115194 26.900513598238955 22.147108471761328 20-21 24.077741133228205 27.341270832419433 26.613627686572194 21.967360347780165 22-23 24.459806719691088 26.88418396256112 26.66941931963652 21.986589998111267 24-25 23.630232097222457 27.611399332854127 26.784344514439073 21.974024055484346 26-27 24.036498670890666 27.776929511178665 26.353684440873742 21.832887377056927 28-29 24.259909419349924 27.03492088606848 26.691032121133873 22.014137573447723 30-31 23.978302473506904 27.052019102472798 26.885578486662965 22.084099937357337 32-33 23.870540141428197 27.364554502430316 26.79719694654054 21.967708409600938 34-35 24.25687267875154 26.986322565485366 26.75410462028427 22.002700135478825 36-37 23.55776200817704 27.02100781889749 27.220141397691656 22.201088775233817 38-39 23.95826908011207 27.020881824846033 26.99643937496145 22.02440972008045 40-41 24.567701648064418 26.57080377462302 26.748813222251716 22.112681355060847 42-43 24.167948365711087 26.711942378287468 27.073554896896525 22.046554359104924 44-45 24.540097285206247 26.815416496060347 26.762154802170627 21.88233141656278 46-47 24.333754764177602 26.697691194739132 26.769466627177852 22.199087413905414 48-49 23.875262129702516 26.665863417102365 26.878563621852287 22.580310831342835 50-51 24.41536297146233 26.913898535931725 26.55443830863664 22.116300183969305 52-53 24.24917494561918 26.89035594360391 26.970510451999996 21.889958658776916 54-55 23.79428910757716 26.979485996503644 26.968337338058003 22.257887557861196 56-57 24.130076099709626 26.930901988031174 27.266904819648275 21.67211709261092 58-59 24.431636799692463 27.23272796976862 26.74282900238389 21.59280622815503 60-61 23.87110771306046 27.475144937063313 26.638495873471406 22.015251476404817 62-63 24.523867029374717 27.52730404436815 26.320499943945187 21.628328982311952 64-65 24.5224731211332 27.428785177074765 26.493404703315232 21.5553369984768 66-67 24.334378754290757 27.56353626948101 26.594024814739146 21.508060161489084 68-69 24.805921864325125 27.54439174093092 26.346177733984856 21.3035086607591 70-71 24.85544806863662 27.517258462424888 26.268280056094756 21.359013412843737 72-73 24.78033403399085 27.528203541227114 26.218932963728843 21.47252946105319 74-75 25.125029532831146 27.56993083218127 26.054623105983392 21.25041652900419 76-77 25.38285508494773 27.473574986390005 26.11985476443779 21.023715164224477 78-79 25.259091901545787 27.44633889978957 26.208858100309136 21.08571109835551 80-81 25.702258346211483 27.600873192647867 25.642705315003116 21.054163146137537 82-83 25.96622568928425 27.52009970437238 25.683725072661336 20.829949533682033 84-85 25.869286755560367 27.518863765175116 25.798967560859122 20.8128819184054 86-87 26.18890261759017 27.650540803611513 25.52136258176036 20.639193997037957 88-89 26.510859682563126 27.53031065245785 25.43773545640414 20.521094208574883 90-91 26.531998371677297 27.528866090179726 25.478136211470463 20.460999326672514 92-93 27.047255064424895 27.70669977088673 24.958698828358354 20.287346336330017 94-95 27.279908799190565 27.56592081368792 25.05479406007479 20.099376327046713 96-97 27.205607865805774 27.76714545675958 25.09855700069112 19.928689676743527 98-99 27.771287481461226 27.818278765026488 24.772487408423107 19.637946345089176 100-101 28.050656562806598 27.694113286216577 24.634478373609692 19.620751777367133 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10981.0 1 6012.5 2 844.5 3 566.0 4 465.0 5 399.5 6 374.0 7 399.5 8 378.5 9 354.5 10 375.0 11 398.0 12 435.0 13 479.0 14 513.5 15 571.0 16 634.0 17 671.5 18 735.0 19 861.0 20 1051.5 21 1307.5 22 1618.5 23 2108.0 24 2896.5 25 4046.0 26 5967.5 27 8798.0 28 12948.0 29 18800.5 30 26249.0 31 35617.5 32 46803.0 33 59473.0 34 74406.0 35 93054.0 36 118650.5 37 150653.5 38 188168.0 39 234705.0 40 292040.5 41 357367.5 42 425061.5 43 493185.0 44 555059.5 45 601894.0 46 631646.5 47 639649.5 48 627156.0 49 597374.0 50 548964.5 51 489462.5 52 422453.0 53 356032.5 54 293720.0 55 239422.5 56 195206.5 57 161051.0 58 134759.0 59 109314.5 60 86990.5 61 62885.0 62 39975.5 63 25562.0 64 16536.5 65 10634.0 66 7493.5 67 4843.0 68 3317.0 69 2692.0 70 2490.0 71 2009.0 72 1190.0 73 904.0 74 875.0 75 723.5 76 508.0 77 237.0 78 110.5 79 78.0 80 67.5 81 57.5 82 49.0 83 35.0 84 32.0 85 27.5 86 19.0 87 19.0 88 20.5 89 17.0 90 11.0 91 8.0 92 7.0 93 5.0 94 3.5 95 3.0 96 3.0 97 2.5 98 3.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16630104730600365 2 0.15107519737319533 3 0.15670897128230873 4 0.14659331032282258 5 0.17480406666325288 6 0.15503349948777684 7 0.1554837825325573 8 0.14266642330438853 9 0.17107614192041948 10-11 0.14212189497116565 12-13 0.13172349814635226 14-15 0.1915273695124241 16-17 0.19953821903002958 18-19 0.1831238312929752 20-21 0.21750765402638386 22-23 0.2026168984524819 24-25 0.19372642624274716 26-27 0.17841156687085433 28-29 0.19587836032884903 30-31 0.1436245837368864 32-33 0.16996614185654213 34-35 0.17481453836196872 36-37 0.17805029326515837 38-39 0.17752670832936718 40-41 0.2123608141075563 42-43 0.1979727000720139 44-45 0.18189340669386586 46-47 0.1857260484238575 48-49 0.22602638093170685 50-51 0.20342845510295826 52-53 0.16845298139210552 54-55 0.15466175418336509 56-57 0.15038930110730886 58-59 0.1648926038287253 60-61 0.16047354697064753 62-63 0.1491169897133362 64-65 0.17085623624738716 66-67 0.17494543459591652 68-69 0.18661614281470254 70-71 0.16793986815503015 72-73 0.17630675542897367 74-75 0.20839204029425895 76-77 0.23606874000018221 78-79 0.21818307859355454 80-81 0.1942290677811067 82-83 0.16508109440561014 84-85 0.173725481695523 86-87 0.16324331128098302 88-89 0.1668874624340898 90-91 0.17844298196700178 92-93 0.16355222639309983 94-95 0.1569131694072673 96-97 0.1646779340050509 98-99 0.15500208439162938 100-101 0.16234274519142214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9549549.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.91425654138469 #Duplication Level Percentage of deduplicated Percentage of total 1 72.40941268831854 34.694431755590905 2 14.063450997127156 13.476795978670856 3 5.3033958479401635 7.62324807596158 4 2.536927837886572 4.862200450059104 5 1.3965490811106909 3.345730547248635 6 0.872141015943099 2.507279302689691 7 0.6152410940224945 2.0635173729657184 8 0.4321421919045466 1.656461747621659 9 0.3269332780980789 1.4098288462836497 >10 1.824838068062398 15.99917958867851 >50 0.14382995731555753 4.696938695556251 >100 0.071517445475061 6.1191818380371386 >500 0.002811344364421339 0.8926060169996815 >1k 7.872058721519216E-4 0.5666878992176353 >5k 2.1946558890098538E-5 0.08591188441872638 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.560377563380218E-4 1.0471698715824172E-5 0.0 2 6.073585255178019E-4 1.0471698715824172E-5 0.0 3 7.539623075393403E-4 1.0471698715824172E-5 0.0 4 0.0016440566983843949 1.0471698715824172E-5 0.0 5 0.0017487736855426367 1.0471698715824172E-5 0.0 6 0.002188585031607252 1.0471698715824172E-5 0.0 7 0.002754056762261757 1.0471698715824172E-5 0.0 8 0.00429339647348791 1.0471698715824172E-5 0.0 9 0.004701792723405053 1.0471698715824172E-5 0.0 10-11 0.005413868236081096 1.5707548073736257E-5 1.0471698715824172E-5 12-13 0.006052641857746371 2.0943397431648344E-5 1.0471698715824172E-5 14-15 0.0069165570018018645 2.0943397431648344E-5 1.0471698715824172E-5 16-17 0.008141745751553294 2.0943397431648344E-5 1.5707548073736257E-5 18-19 0.010356510029950104 4.188679486329669E-5 2.0943397431648344E-5 20-21 0.012408962978251644 4.188679486329669E-5 2.0943397431648344E-5 22-23 0.01473891594252252 4.188679486329669E-5 2.0943397431648344E-5 24-25 0.01782283121433274 6.283019229494503E-5 2.0943397431648344E-5 26-27 0.022482737142874496 7.33018910107692E-5 2.0943397431648344E-5 28-29 0.03212193581079065 7.33018910107692E-5 2.0943397431648344E-5 30-31 0.05006519156035537 7.33018910107692E-5 2.0943397431648344E-5 32-33 0.07415009860675095 7.33018910107692E-5 2.0943397431648344E-5 34-35 0.10511491170944304 7.33018910107692E-5 2.0943397431648344E-5 36-37 0.1491169897133362 8.900943908450546E-5 2.0943397431648344E-5 38-39 0.2101617573772332 1.1518868587406588E-4 2.0943397431648344E-5 40-41 0.29040638463659385 1.1518868587406588E-4 2.6179246789560428E-5 42-43 0.39426469250013796 1.1518868587406588E-4 3.1415096147472515E-5 44-45 0.5185061619140339 1.1518868587406588E-4 3.1415096147472515E-5 46-47 0.6620417362118358 1.1518868587406588E-4 3.1415096147472515E-5 48-49 0.8317513214498402 1.1518868587406588E-4 3.1415096147472515E-5 50-51 1.0298025592622229 1.1518868587406588E-4 3.1415096147472515E-5 52-53 1.256342053431005 1.1518868587406588E-4 3.1415096147472515E-5 54-55 1.5119771624817047 1.1518868587406588E-4 3.1415096147472515E-5 56-57 1.8007761413654195 1.1518868587406588E-4 3.1415096147472515E-5 58-59 2.1231316787839924 1.1518868587406588E-4 3.1415096147472515E-5 60-61 2.4882379262099183 1.1518868587406588E-4 3.1415096147472515E-5 62-63 2.903723516157674 1.1518868587406588E-4 3.1415096147472515E-5 64-65 3.365991420118374 1.2042453523197797E-4 3.1415096147472515E-5 66-67 3.879439751552665 1.2566038458989006E-4 3.1415096147472515E-5 68-69 4.450430067430409 1.2566038458989006E-4 3.1415096147472515E-5 70-71 5.080051424418054 1.2566038458989006E-4 3.1415096147472515E-5 72-73 5.787624106646293 1.2566038458989006E-4 3.1415096147472515E-5 74-75 6.566179198619746 1.2566038458989006E-4 3.1415096147472515E-5 76-77 7.415502030514739 1.3613208330571423E-4 3.1415096147472515E-5 78-79 8.34620566897976 1.4136793266362633E-4 3.1415096147472515E-5 80-81 9.371720067617854 1.466037820215384E-4 3.1415096147472515E-5 82-83 10.479720037040494 1.466037820215384E-4 3.1415096147472515E-5 84-85 11.679425907966962 1.5707548073736258E-4 3.66509455053846E-5 86-87 12.957439141890365 1.5707548073736258E-4 4.188679486329669E-5 88-89 14.297397709567226 1.5707548073736258E-4 4.188679486329669E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGGGG 4240 0.0 34.56646 38-39 GGGGCGC 2590 0.0 33.938293 42-43 GGCGCCC 900 0.0 32.99599 44-45 GGGCGCC 3380 0.0 30.012295 42-43 AAGGGGC 1840 0.0 29.430593 9 GGGCCCG 670 0.0 29.430454 44-45 GGGGGCG 3315 0.0 28.66259 40-41 GTAGGGG 2175 0.0 27.961956 18-19 CTCGGGG 3605 0.0 27.345102 36-37 AGGGTAG 3355 0.0 27.25275 28-29 GATCGGG 3685 0.0 25.6443 1 GAGCGGC 3245 0.0 25.324648 9 CGGGTAG 1820 0.0 24.669622 14-15 GGGTAGA 3895 0.0 24.389038 28-29 GAGGGGC 1570 0.0 23.599726 9 GGGTAGG 1980 0.0 23.397158 16-17 GGGGGGC 3885 0.0 22.989832 40-41 GGGCGGG 1925 0.0 22.949507 12-13 AGAGCGG 3610 0.0 22.8879 8 CGGGGGG 4200 0.0 22.73591 38-39 >>END_MODULE