FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n02 tsrexp_lib#1.3420000000d055.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n02 tsrexp_lib#1.3420000000d055.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9549549
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG42400.034.5664638-39
GGGGCGC25900.033.93829342-43
GGCGCCC9000.032.9959944-45
GGGCGCC33800.030.01229542-43
AAGGGGC18400.029.4305939
GGGCCCG6700.029.43045444-45
GGGGGCG33150.028.6625940-41
GTAGGGG21750.027.96195618-19
CTCGGGG36050.027.34510236-37
AGGGTAG33550.027.2527528-29
GATCGGG36850.025.64431
GAGCGGC32450.025.3246489
CGGGTAG18200.024.66962214-15
GGGTAGA38950.024.38903828-29
GAGGGGC15700.023.5997269
GGGTAGG19800.023.39715816-17
GGGGGGC38850.022.98983240-41
GGGCGGG19250.022.94950712-13
AGAGCGG36100.022.88798
CGGGGGG42000.022.7359138-39
Produced by FastQC (version 0.11.2)