FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n01 tsrexp_lib#7.3410000000d071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n01 tsrexp_lib#7.3410000000d071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14352504
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC183330.12773380867895945TruSeq Adapter, Index 5 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCCC3652.2573658E-916.9407141
CGGGGCG3502.4663677E-816.3077641
CGCGTAT13600.014.6889791
GGCGCGC9400.012.8884738-39
CGGGGCT17900.012.4889551
CGCGACT14500.012.4651311
CGCCAAT48250.012.1252031
CGCGGAT23600.012.0926231
CGGGGAC59050.011.4203372
CCGGGTT65850.011.3403151
CGGGCGT8156.366463E-1111.08861351
CGGGCCT9900.011.0502961
CGGGCAT24100.010.6575651
CTGGGGT24650.010.6127281
CCCGACT20700.010.5698481
CCCGTGT18100.010.5114511
CCCGGGT48000.010.4046941
CGGGGTT30200.010.3948511
CTCGGTT60250.010.3417851
CTCGGAT67000.010.2227781
Produced by FastQC (version 0.11.2)