FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n01 tsrexp_lib#4.3410000000d064.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n01 tsrexp_lib#4.3410000000d064.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12330407
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC245030.19872012335034844TruSeq Adapter, Index 4 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCCC2701.6602359E-414.0926931
GGCGCGC7950.013.74560738-39
CGGGGGT7700.013.5893841
CGCGTAT9900.013.4521161
CGGGGTT24750.012.491251
CGCGGGT14400.011.890711
CGGGGAC45200.011.3472442
CGGGAGT36600.011.1759691
CGCGGAT21300.011.1649871
CGGGGCA10650.011.1649871
TATGCCG120300.010.97655846-47
GCCGTCT124350.010.81116648-49
ATGCCGT124550.010.77362246-47
TGCCGTC124700.010.76177548-49
CGCGAAT22100.010.7608221
CCGCGGT13550.010.5305191
TGAAAAA130750.010.372807562-63
CGCGCAA7854.438334E-910.3002331
CGCGCGT6051.6693139E-610.2201151
CGCGCAC10700.010.21213240-41
Produced by FastQC (version 0.11.2)