FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n01 tsrexp_lib#38.3410000000d106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n01 tsrexp_lib#38.3410000000d106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6584223
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT13200.027.7447911
CCGGGGT4650.018.411291
CGGCCCT5700.015.8541661
CGCGGCT12200.015.5942621
CCGGCCT11650.015.51394751
CGCGCGT6000.015.0614581
CGGATGG21000.014.9260793
CGGGGTA6700.014.9076491
CGCGTAT6100.014.81454851
GCGGATG23100.014.8027212
CCGGGCT9450.014.0925921
CCCGTGT8150.014.0061351
CGCGCCT7500.013.9516661
CGGGGGT5652.8012437E-1013.4690271
CCCGACT11300.013.4690271
CCCGCCT11100.013.283221
CCCGTTT15100.013.2293041
GCCGAAT12750.013.0563731
CCGCGGT10550.012.6232221
ATGGACG25700.012.5654216
Produced by FastQC (version 0.11.2)