FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n01 tsrexp_lib#35.3410000000d0f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n01 tsrexp_lib#35.3410000000d0f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8299067
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC195380.23542405429429597TruSeq Adapter, Index 10 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT17350.032.9007381
GCGGATG28800.018.96322
CGGATGG28000.017.8089183
CGGCCCT4700.017.2058031
CCGGGGT5850.016.26291
ATGGACG31950.015.3088966
CGCGCGT8550.015.0217841
CGCGGCT15350.014.8749911
CGCGTGT9400.014.6755371
CGCGCCT10000.014.2706941
CGCGCAT7950.013.7620951
CCGGGTT32150.013.6122741
CCCGACT17450.013.357481
CGCGTAT7550.013.2311081
CCGCGGT13450.013.0858911
GCCGATT19650.013.07239151
CCGGAAC34900.012.5396751
CCCGGGT8800.012.4328021
CGGGAAT23800.012.3919191
CGCGCGA2700.002646979212.33271
Produced by FastQC (version 0.11.2)