FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n01 tsrexp_lib#32.3410000000d0eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n01 tsrexp_lib#32.3410000000d0eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9726134
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC390540.40153672569183196TruSeq Adapter, Index 9 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT17400.028.4306141
CGCGGCT17350.015.627071
CGGATGG29700.014.5504533
GCGGATG35950.014.2664682
CGCGTAT8000.013.67541
CGGGGGT7400.013.4986321
GCGGGGT6451.2732926E-1113.2744031
CGCCCCC4052.2661243E-612.9193261
TGAAAAA156550.012.684010562-63
CGGGGGA12150.012.5278311
CGCGCCT11700.012.1965651
CGCGGTT17300.012.0978661
GCGGACT8900.011.7580381
ATGGACG36400.011.7413856
GAAAAAA174750.011.60764462-63
TTGAAAA175350.011.59600260-61
CCGGGTA16000.011.594361
CCCGACT19900.011.4733521
CGGGAAT29100.011.4421391
CTGCTTG179600.011.28177456-57
Produced by FastQC (version 0.11.2)