FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n01 tsrexp_lib#29.3410000000d0de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n01 tsrexp_lib#29.3410000000d0de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5909014
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC362320.6131649036539768TruSeq Adapter, Index 8 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT6700.024.1330641
CGCGGAT9700.023.533031
CCGGGGT4450.023.5109961
CCCGGGT6850.019.4390851
CGCGCCT7650.019.2711931
GCGGCCC7400.018.610928
GCGGGTT15050.018.011371
TGAAAAA108850.016.47460762-63
CGGGGAT17350.016.4459931
GAAAAAA117400.015.90197262-63
CCGGGTT25150.015.6945351
CCCGCTA4551.5643309E-1015.6779111
GCGGGGT3704.893809E-815.4236761
CGGGGGT5651.8189894E-1215.150691
CGGGCAT9300.014.8293931
TTGAAAA127050.014.60203360-61
CCCGTGT8500.014.5466421
CCGGGAT18650.014.5346421
CGGGGCG2307.920902E-414.4736671
GCCCGCT3951.0906115E-714.4474931
Produced by FastQC (version 0.11.2)