FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n01 tsrexp_lib#24.3410000000d0c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n01 tsrexp_lib#24.3410000000d0c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8652119
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC258680.2989787819608121TruSeq Adapter, Index 3 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT17500.021.197631
CCGGGGT6800.020.981821
CGCGCCT12800.019.3207571
CGCGCGT11400.018.7732051
CGGGGGA11400.018.7732051
GCCCCCT6350.017.9749741
GCGGGTT18850.017.4087871
CGGGGGT6800.016.0860611
CCCGGGT10100.016.0098881
GCGGGGT6300.015.8529281
CCCGTGT13850.015.4523121
GCGGCCC11300.014.2888018
CCCGTAT12400.014.19092751
GCGCCTG16100.014.1588592
CGGGCGT7400.014.1390981
CGCCCGT8600.013.8252281
GTCGGCA22150.013.74159051
CCCCCCT5301.5552359E-913.4600331
GCGGGAT15550.013.4571481
CGGGTGT9900.013.450971
Produced by FastQC (version 0.11.2)