FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n01 tsrexp_lib#21.3410000000d0b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n01 tsrexp_lib#21.3410000000d0b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12486195
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC707170.5663614896291465TruSeq Adapter, Index 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT27000.025.892021
CCGGGGT9150.022.3490831
CGGGGGT11000.018.158041
CGCGCCT20300.018.0387761
CCCGTGT18000.017.7016871
TGAAAAA215550.017.27763762-63
CCCGGGT14000.016.3051781
GAAAAAA236000.015.91131962-63
GCGGATG43000.015.793732
GCGGGGT8450.015.7584561
GCCGGGT16950.015.7119711
CGCGCGA4601.8553692E-1015.5076421
TTGAAAA244450.015.47941960-61
CTGCTTG244450.015.43055456-57
CCGGGCT20000.014.9803821
CGCGCGT15350.014.8711731
GCTTGAA253650.014.79617358-59
CGGGTGT15600.014.63285351
CTTGAAA257200.014.53659560-61
CGGGGGA16000.014.2670311
Produced by FastQC (version 0.11.2)