FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n01 tsrexp_lib#18.3410000000d0a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n01 tsrexp_lib#18.3410000000d0a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22420129
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1912530.8530414789317224TruSeq Adapter, Index 12 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT21050.023.7246841
TGAAAAA458750.021.44577862-63
GCCGTCT467900.020.925148-49
CTGCTTG476750.020.90185756-57
CGCGGAT45700.020.8150311
TGCCGTC471600.020.61990548-49
ATGCCGT477100.020.45530346-47
GAAAAAA481100.020.43961762-63
GCGGGTT55800.020.2864481
TATGCCG493000.019.73777846-47
TTGAAAA502900.019.650260-61
GCTTGAA516050.019.12176758-59
CTTGAAA530000.018.56028460-61
TGCTTGA538350.018.42676758-59
GTATGCC540700.018.18010944-45
GCGGGGT14250.018.0236241
CGTATGC543850.017.92196744-45
CCGTCTT564800.017.6084450-51
GCCGGGT30950.016.9042261
CCCGGGT28400.016.5798321
Produced by FastQC (version 0.11.2)