FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n01 tsrexp_lib#15.3410000000d09b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n01 tsrexp_lib#15.3410000000d09b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21077441
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1045800.496170289362926TruSeq Adapter, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT18450.026.5519981
CGCGGAT37700.022.9607681
CCCGGGT23000.019.2314261
CGCGCGT22950.017.6154041
GCGGGTT50300.016.3581621
TGAAAAA332950.015.82288262-63
CGGGGGT18600.015.3424481
CGGGGGA26200.015.0672221
GCGGGGT12950.015.058111
GCCGGGT28150.015.0372351
GAAAAAA360900.014.715939562-63
CGGGGTG11900.014.3668772
CTGCTTG389350.014.01349656-57
TTGAAAA387800.013.91063360-61
GCCGTCT383800.013.73235648-49
CGGGCAT36350.013.6077181
CCGGCCT44100.013.58902551
CCGGGTT79850.013.461391
CGGGGAT55650.013.4180941
TGCCGTC394200.013.37608748-49
Produced by FastQC (version 0.11.2)