FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n01 tsrexp_lib#10.3410000000d08e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n01 tsrexp_lib#10.3410000000d08e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9509734
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC128070.13467253658199063TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCCG1350.00934088417.6135981
GGCGCGC6600.015.4771738-39
CCCCGAT7050.014.8403931
CGGGCCT7850.014.5396331
CGCGTAT8250.014.4111271
CCCGTCT15750.013.5876321
CGGGGCG2500.001487100313.3158811
GCGCGCA9250.012.07044138-39
GGGGTAT15800.011.7222663
GGGTATA17100.011.664264
CTGGGGT16550.011.494071
GCCGTAC10800.011.43240455
CGCCTAT8451.0913936E-1111.2560271
CTGGAAT59050.011.1140161
CGGGGCT11650.011.02172851
CGCGCAC9650.010.83088140-41
CGGCAAT27150.010.6849351
CCGGACT13900.010.6061741
CGGGCAT16600.010.5999911
CCGGGTT42800.010.5558131
Produced by FastQC (version 0.11.2)