FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l02n01 tsrexp_lib#1.3410000000d058.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l02n01 tsrexp_lib#1.3410000000d058.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9549549
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC591990.619914092278075TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA149700.021.6259962-63
CGGGCCC2551.17524905E-820.519251
GCCGTCT157850.020.23766148-49
TATGCCG160100.019.84799646-47
TGCCGTC161050.019.79130248-49
ATGCCGT163650.019.44646646-47
CTGCTTG170050.019.22083356-57
TTGAAAA177450.018.19238960-61
GAAAAAA181150.017.96321962-63
GTATGCC179050.017.77318844-45
CCGTCTT182300.017.67973550-51
TGCTTGA187050.017.5760558-59
CGTATGC189300.016.86100844-45
CTTGAAA201650.016.33896660-61
TCGTATG210300.015.22419642-43
TTCTGCT220300.014.89992754-55
GCTTGAA221150.014.82296458-59
CTCGTAT220600.014.47029942-43
CTTCTGC226950.014.42147354-55
CGTCTTC233700.013.98435250-51
Produced by FastQC (version 0.11.2)