FastQCFastQC Report
Fri 20 Mar 2015
lane8_Undetermined_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane8_Undetermined_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165260567
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26212481.5861303440886778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAACT1577800.01337.91821
GAAACTC1008550.0702.183532
GAAACTG1292850.0647.191162
GAAACTA748500.0484.060642
AAACTCG423350.0482.554083
AAACTGC611800.0457.431583
AAACTCC562100.0371.680423
AACTCGC221150.0353.99224
AAACTGG757000.0351.890143
AAACTGT662150.0341.94883
AACTGCC388700.0325.04624
AAACTCT763750.0319.00553
AAACTAG445450.0268.382233
AACTCCC335150.0261.70274
AACTGGC496250.0260.275024
AACTCCG212300.0258.920044
TGAAATT1079700.0253.404041
AACTCGG267100.0250.078674
AACTGTC343050.0240.134084
AACTGCG274200.0235.387284
Produced by FastQC (version 0.11.2)