FastQCFastQC Report
Fri 20 Mar 2015
lane5_Undetermined_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane5_Undetermined_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161407008
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26401981.6357393849962203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAACT880050.01720.84991
GAAACTC549600.0953.40312
GAAACTG748700.0869.16682
AAACTGC420750.0546.592163
GAAACTA520850.0543.053342
AAACTCG268450.0516.22953
AAACTCC413750.0387.557923
AAACTGG554350.0370.919043
AAACTGT505850.0364.77223
GAACCTC257250.0325.380832
AAACTCT597750.0320.242773
AACTGCC361300.0315.800574
AACTCGC189650.0312.41094
GAACCTG353600.0285.528352
TGACACT326100.0280.0811
TTACACT302750.0275.897831
AAACTAG359150.0255.549153
TGAAGCT384700.0247.055981
AACTCCC289200.0246.53314
AACTGGC462950.0240.01574
Produced by FastQC (version 0.11.2)