FastQCFastQC Report
Fri 20 Mar 2015
lane4_Undetermined_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane4_Undetermined_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216190337
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAT11314950.044.034181
TTACGCT11160700.043.95971
TACGCTG4563700.043.2756772
GTCTATG4804550.043.2738152
TACGCTC4345100.043.2538762
CACAGTG4151700.043.1781122
GAACGTT11143700.042.9853741
TCACAGT10594350.042.8150061
TGAAACT10888950.042.7854161
CTCAGAT6782100.042.671811
CGAAATG4009950.042.663272
ACAAGTT10341800.042.376921
GTCTATC4056300.042.0981372
GATCAGT7857000.042.071261
GCTCCCG623600.041.8282785
ACGCTCC1461050.041.6209263
TCGAAAT10196500.041.5999831
GTGGCCT7512150.041.5528261
GACTTAT6288550.041.485131
ACGCTGC1712650.041.348813
Produced by FastQC (version 0.11.2)