FastQCFastQC Report
Fri 20 Mar 2015
lane3_Undetermined_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane3_Undetermined_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230989923
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCAT13724150.043.3846321
GCTTCAT3954500.038.563211
TCTGCAT3537350.036.7052231
CTTCATG2366150.034.2988172
GATGCAT2094050.033.664981
ATGCATC1491950.031.4291822
GCAAGAT1644450.030.5076227
GCCTCAT888600.030.0568731
AAGATCG1920800.030.0495629
TATGCAT1265700.030.018341
CTGCAAG1153750.029.7873481
ATGCATG1652950.029.40512
CTTCATC2668350.029.181582
AGATCGG1964750.028.85476710
GCCGCAT1153050.028.7529961
GCTGCTT1948700.028.634041
GCAGCAT904700.027.8130111
CTGCCTG1224400.027.2789272
GCATCAT1794350.026.9015941
CTGCAAC1208850.026.8030051
Produced by FastQC (version 0.11.2)