FastQCFastQC Report
Fri 20 Mar 2015
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3690718
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG39670.10748586047484526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG3750.026.95122511
CGTCTGA3550.026.9289716
ACGTCTG3850.024.686115
CACGTCT3950.024.44528214
CACACGT4150.023.96728512
ACACGTC4100.023.38557613
GCTGACT7550.023.0410351
ACTCTAG7400.022.0731265
AGCACAC4750.020.0347410
CTGACTC8500.019.7883552
CAGTCAC4150.019.28107327
AGAGCAC4850.019.1631588
CTAGCAG7950.018.3711518
CTCTAGC9200.017.2969046
TAGCAGA9500.016.7706539
TCGGAAG6200.016.4609093
CCAGTCA4950.016.17418926
CTGAACT6500.015.16311819
ATCGGAA6850.014.9880522
GACTCTA12200.014.6168274
Produced by FastQC (version 0.11.2)