FastQCFastQC Report
Fri 20 Mar 2015
9_GATCAG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename9_GATCAG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13519276
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG405040.299601842583878No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG284660.21055861275411494No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG258020.19085341552313895No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA181840.1345042441621874No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC148900.11013903407253467No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC143810.10637403955655612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16700.023.70934911
GCTGACT56500.023.5623631
GTATGCC18850.021.23680145
CGTATGC19500.020.52913544
ACGTCTG20700.019.67138315
GACTCTA69750.019.6512874
ACTCTAG70800.019.232695
CTAGCAG71400.018.7200288
CTCGTAT21450.018.66906542
CTGACTC72050.018.599932
AGCACAC22400.017.77771810
ACACGTC23500.016.9444413
AGTCACG22400.016.87326628
GTCACGA23200.016.87176729
CACGTCT24600.016.5523214
TCGTATG24550.016.30991743
TGACTCT84700.016.1821333
CTCTAGC84800.016.1600576
TAGCAGA83600.016.1214879
CGTCTGA25550.015.84858916
Produced by FastQC (version 0.11.2)