FastQCFastQC Report
Fri 20 Mar 2015
8_ACTTGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8_ACTTGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15707713
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG438300.2790348919667682No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG314830.20043019629910475No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG308850.19662314940437223No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA185550.11812668082234505No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC169500.10790877067845586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG20000.023.284111
GCTGACT69250.023.0624081
GTATGCC20450.020.56344645
ACTCTAG83150.019.8135175
GACTCTA85800.019.6474974
CGTATGC21900.019.20236644
ACGTCTG24250.018.92543215
CTAGCAG85800.018.8568258
CTGACTC87700.018.7216952
AGCACAC26300.017.53629910
CACACGT26200.017.25892812
CTCGTAT25200.016.9610542
CTCTAGC98650.016.856776
TGACTCT101350.016.4769823
ACACGTC27350.016.20421413
TAGCAGA102000.015.9481799
CACGTCT29400.015.53329714
TCGTATG28950.014.99599443
CTATCGA110900.014.40216917
GAGCACA33150.014.0494669
Produced by FastQC (version 0.11.2)