FastQCFastQC Report
Fri 20 Mar 2015
7_CAGATC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename7_CAGATC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16462111
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG566300.3440020541715458No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG398720.24220466014352593No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG354540.2153672758007767No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA250190.15197929354260822No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC203070.12335599000638496No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC191370.1162487605629679No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT177200.10764111601482945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG25800.028.33964711
GTATGCC27150.026.67168845
CGTATGC27600.026.23738344
GCTGACT77100.024.1034761
AGCACAC32200.022.77803210
CTCGTAT32100.022.77641342
CACACGT32700.022.4285212
ACGTCTG34050.021.73749715
ACACGTC34000.021.43862213
ACTCTAG90400.021.0624835
CACGTCT35100.020.89443814
TCGTATG36050.020.7161743
GACTCTA94050.020.556164
CTGACTC93950.020.0654092
CTAGCAG95100.019.8993388
CGTCTGA39050.019.12605516
GAGCACA39800.018.7690039
CAGTCAC41550.017.97860127
CTCTAGC107150.017.9548826
AGAGCAC42250.017.8419068
Produced by FastQC (version 0.11.2)