FastQCFastQC Report
Fri 20 Mar 2015
6_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6_GCCAAT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14058468
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG538530.38306449891979694No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG378300.26909048695775384No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG324510.23082884991451413No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA243350.17309851969645626No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC228620.1626208488720108No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC180630.1284848391730877No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT161050.11455729031072234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT70600.023.1003881
GCACACG17300.022.23633811
CGTATGC16700.020.33491744
ACTCTAG80800.020.2512195
GACTCTA84300.019.7575114
CTGACTC82800.019.6943912
ACACGTC19850.018.8131213
ACGTCTG20750.018.53960815
CACACGT21300.017.84926612
GTATGCC19350.017.78184945
CTAGCAG90700.017.7628128
AGTCACG20350.017.13662528
TGACTCT98000.017.1098383
TCGTATG19900.016.95612743
CTCTAGC97550.016.839036
CACGCCA20750.016.47812731
CTCGTAT21700.016.06926542
AGCACAC25050.015.98632710
GTCACGC21700.015.75796729
TAGCAGA104000.015.7280969
Produced by FastQC (version 0.11.2)