FastQCFastQC Report
Fri 20 Mar 2015
5_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5_ACAGTG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14027497
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG589210.4200392985291674No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG406250.2896097571790605No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG372070.2652433288704321No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA260010.18535737345016007No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC218970.15610055022645877No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC185960.13256819801850608No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT184490.13152025625099045No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG144030.10267690664984637No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT143570.10234897929402514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT75250.024.9939211
CTGACTC83100.022.954462
ACTCTAG83200.022.7217375
GACTCTA87600.022.0172084
CTAGCAG94650.020.0645038
CTCTAGC97900.019.4447336
TGACTCT102850.019.1456763
GCACACG14000.018.64036411
TAGCAGA106500.018.0846049
CGTATGC14400.017.33617644
CACACGT16150.016.4374312
GTATGCC16400.015.3588745
CTATCGA123950.015.0999317
TCTATCG123450.015.08862916
ACGTCTG17450.014.6974915
ACACGTC17650.014.40317313
TATCGAT131600.014.31033418
GCAGATC133650.014.2405211
AGATCTA134300.014.03758713
GATCTAT134950.013.91981314
Produced by FastQC (version 0.11.2)