FastQCFastQC Report
Fri 20 Mar 2015
4_TGACCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4_TGACCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14438186
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG639020.4425902256696236No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG439360.3043041556605518No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG405740.2810186819867814No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA275310.19068184881397152No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC231500.1603387018286092No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC213970.14819728738776464No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT202470.14023229788007993No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG158310.10964673817057072No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT154050.10669622901381103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT81750.024.2184811
GCACACG16550.023.1082311
ACTCTAG87150.023.0100335
CTGACTC88350.022.4573172
GACTCTA91200.022.2349874
CGTATGC16650.021.74640744
CACACGT18800.020.22295612
CTAGCAG100000.019.8919738
ACGTCTG19250.019.75068915
CTCTAGC102300.019.4444086
GTATGCC19100.019.0743645
ACACGTC20550.018.93869613
TGACTCT108200.018.657723
TAGCAGA112850.017.605399
CTCGTAT22100.016.89773842
AGCACAC23800.016.54231810
TCGTATG22650.016.38673243
CACTGAC25250.015.14471831
ACTGACC24700.015.02659732
CACGTCT25800.014.99764314
Produced by FastQC (version 0.11.2)