FastQCFastQC Report
Fri 20 Mar 2015
2_CGATGT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2_CGATGT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20808978
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG749980.3604117415088814No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG531150.25525040201397686No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG495960.23833943214318357No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA326050.15668717608332328No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC288450.13861805226570956No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC243520.11702641042726845No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT240140.11540211153089786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT104000.023.6284561
ACTCTAG114900.021.6448695
CTGACTC117000.021.0769312
GACTCTA122050.020.5981454
CTAGCAG131300.018.852358
CTCTAGC136750.018.2813636
TGACTCT142350.017.881473
TAGCAGA147700.016.6811589
GCACACG18800.014.71885811
CTATCGA178450.013.63971117
TCTATCG178000.013.62400216
CGTATGC19650.013.505074544
GTATGCC20400.013.00825245
AGATCTA188950.012.97796113
GATCTAT188350.012.899549514
GCAGATC189650.012.83515811
TATCGAT195000.012.61149818
AGCAGAT194150.012.58459110
ATCTATC192950.012.58069115
ACACGTC23800.012.28834113
Produced by FastQC (version 0.11.2)