FastQCFastQC Report
Fri 20 Mar 2015
1_ATCACG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1_ATCACG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15651387
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG624420.3989550574655141No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG487040.3111800890234201No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG415110.2652225007278908No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA244090.15595422948777637No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT225530.1440958555302479No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC219320.14012815605415674No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC205760.13146438714984174No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG181480.115951385011437No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT170670.10904464888638944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT93850.026.1822781
CTGACTC101950.024.1651042
ACTCTAG102100.024.00455
GACTCTA103100.023.9244754
CTAGCAG111250.021.7431568
CTCTAGC116700.021.1323056
TGACTCT118950.021.0386073
TAGCAGA124300.019.7310459
CTATCGA143600.016.7307117
TCTATCG144500.016.64260516
GCAGATC153750.015.80309911
AGATCTA154700.015.6915113
TATCGAT156200.015.55726618
GATCTAT155200.015.55383414
AGCAGAT158600.015.53355710
ATCTATC156750.015.51534715
CAGATCT183500.013.38813912
ATTCTAG188800.012.98083223
TAGCATG188700.012.961205527
CTAGCAT188450.012.95534926
Produced by FastQC (version 0.11.2)