FastQCFastQC Report
Fri 20 Mar 2015
12_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename12_CTTGTA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14257738
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG587790.4122603459258404No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG463850.3253321108860326No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG250080.17539949184085163No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA239930.16828055053333144No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC218990.15359378886047703No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT146970.10308086738583638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG24100.029.5912511
CGTATGC23800.029.20026644
GTATGCC26000.026.81532145
CTCGTAT27900.025.07604242
AGCACAC29850.023.74161110
ACACGTC29900.023.7006613
CACACGT30800.023.44635812
ACGTCTG31550.023.10291115
TCGTATG30500.022.71570843
GCTGACT55600.021.6333711
CACGTCT33950.021.27055214
CGTCTGA35500.020.27782616
AGAGCAC37300.019.6059828
TCCAGTC37100.019.34912525
GAGCACA38300.018.9166959
CAGTCAC37700.018.20398527
GACTCTA69650.017.7725034
ACTCTAG69400.017.7392355
CTGAACT41900.017.66952519
CTGACTC69050.017.5481192
Produced by FastQC (version 0.11.2)