FastQCFastQC Report
Fri 20 Mar 2015
11_GGCTAC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename11_GGCTAC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12706124
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG403160.31729581735547363No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG278360.219075463138877No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG242390.19076627931539156No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA167740.1320150818613135No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC145800.11474781766650476No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC136410.10735768043818869No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT129690.10206889213421812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18100.027.840611
CGTATGC19500.024.33533144
GTATGCC20450.023.09459345
GCTGACT52450.022.5482671
CTCGTAT21800.021.56821842
CGGCTAC22750.021.5552733
AGTCACG22400.021.49410428
AGCACAC23750.021.40842610
ACGGCTA23050.020.68885432
ACGTCTG24350.020.14088815
ACACGTC25100.019.5385313
CACGGCT24500.019.46441331
TCGTATG24300.019.07002443
ACTCTAG63800.018.5926025
CACGTCT25550.018.5777814
GACTCTA64700.018.5774974
CACACGT26800.018.29914712
CTGACTC65200.018.1020722
CTAGCAG66950.017.7467568
GGCTACA26700.017.60827434
Produced by FastQC (version 0.11.2)